Suryaram Gummuluru | Profiles RNS

Suryaram Gummuluru, PhD

Associate Professor

Boston University School of Medicine

Dept of Microbiology

PhD, University of Rochester
MS, University of Rochester

Websites

	Microbiology Faculty
	Immunology Training Program Faculty

ORCID : 0000-0002-8606-8481

Our research is broadly focused on the role of dendritic cells (DCs) in the initiation and propagation of HIV-1 replication. Since dendritic cells are believed to be the first immune competent cells to encounter virus in the genital mucosa, a thorough understanding of the HIV-DC interactions is of paramount importance. DCs can capture virus particles independently of CD4 and co-receptor complexes, and retain them in an infectious state for an extended period of time. These virus-bearing DCs may then facilitate a more efficient spread of virus to replication-permissive CD4+ T cells. Our recent work has identified a novel glycosphingolipid dependent mechanism of virus attachment to DCs. The fate of the virus particle post-attachment in DCs remains unclear. Virion trafficking within the DC bypasses conventional endocytic organelles, i.e., endosomes and lysosomes. Virus localization within this novel vesicular compartment not only has the potential to protect the invading HIV from being degraded, but also creates a latent reservoir of virus, which could present a major challenge for eradication by antiretroviral therapy. Furthermore, the mechanism of subsequent return of infectious virus particles to the cell surface and the method of subsequent transmission to T cells remains unclear. Current studies utilizing biochemical and microscopic approaches are underway to monitor HIV-1 trafficking and localization in the DC and its subsequent transfer to T cells.

Graduate Faculty (Primary Mentor of Grad Students)
Boston University School of Medicine, Graduate Medical Sciences

BU Sponsored Research
NIH RePORTER Sponsored Research

Providence/Boston Center for AIDS Research (CFAR): Core Support (Cores A, B, E, G, K, SWG)
07/01/2018 - 06/30/2023 (PI of Sub-Project / SP)
PI: C. Robert Horsburgh Jr, MD
The Miriam Hospital NIH NIAID
2P30AI042853-20A1

Persistent HIV expression induced type 1 IFN responses and inflammaging
08/01/2018 - 04/30/2023 (PI)
NIH/National Institute on Aging
1R01AG060890-01

Signals that establish and maintain HIV latency
05/08/2018 - 04/30/2023 (PI)
Boston Medical Center Corporation NIH NIAID
1R01AI138960-01A1

GM3 Nanoparticles for Sustained Delivery of Anti-Retrovirals to Lymphatic Tissues
11/08/2017 - 10/31/2022 (Multi-PI)
PI: Suryaram Gummuluru, PhD
NIH/National Institute of Allergy & Infectious Diseases
5R01AI132111-02

Mechanism of cell-associated HIV-1 transmission
12/01/2015 - 11/30/2020 (PI)
NIH/National Institute of Allergy & Infectious Diseases
5R01AI064099-14

Engineering a 3D human neurovascular unit for investigating and countering neuroinflammatory mechanisms of HIV
09/30/2017 - 08/31/2019 (Co-PI)
NIH/National Institute of Neurological Disorders & Stroke
5R21NS105837-02

CD1a vaginal dendritic cells and HIV-1 acquisition in the female genital tract
05/15/2015 - 04/30/2019 (PI)
Boston Medical Center Corporation NIH NIAID
5R01AI122209-04

Lifespan/Tufts/brown Center for AIDS Research (CFAR): Core A, Core B, Core G and SWG1
07/01/2016 - 06/30/2018 (PI of Sub-Project / SP)
PI: C. Robert Horsburgh Jr, MD
The Miriam Hospital NIH NIAID
3P30AI042853-19S1

A novel microfluidic system to investigate mechanism of HIV-induced disruption of BBB function
01/01/2017 - 12/31/2017 (Co-PI)
The Miriam Hospital NIH NIAID
4P30AI042853-19

Identification of Signals Required for the Establishment of HIV infection and Latency
09/02/2015 - 08/31/2016 (PI)
Boston Medical Center Corporation NIH NIAID
1R56AI118682-01

Showing 10 of 12 results. Show All Results

Yr	Title	Project-Sub Proj	Pubs
2019	GM3 Nanoparticles for Sustained Delivery of Anti-Retrovirals to Lymphatic Tissues	5R01AI132111-02	1
2019	Mechanism of cell-associated HIV-1 transmission	5R01AI064099-14	22
2018	Persistent HIV expression induced type I IFN responses and inflammaging	1R01AG060890-01
2018	GM3 Nanoparticles for Sustained Delivery of Anti-Retrovirals to Lymphatic Tissues	1R01AI132111-01A1	1
2018	Engineering a 3D human neurovascular unit for investigating and countering neuroinflammatory mechanisms of HIV	5R21NS105837-02
2018	Mechanism of cell-associated HIV-1 transmission	5R01AI064099-13	22
2017	Engineering a 3D human neurovascular unit for investigating and countering neuroinflammatory mechanisms of HIV	1R21NS105837-01
2017	Mechanism of cell-associated HIV-1 transmission	5R01AI064099-12	22
2016	Mechanism of cell-associated HIV-1 transmission	2R01AI064099-11A1	22
2014	Mechanism of cell-associated HIV-1 transmission	5R01AI064099-10	22

Showing 10 of 23 results. Show All Results

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

Xu F, Bandara A, Akiyama H, Eshaghi B, Stelter D, Keyes T, Straub JE, Gummuluru S, Reinhard BM. Membrane-wrapped nanoparticles probe divergent roles of GM3 and phosphatidylserine in lipid-mediated viral entry pathways. Proc Natl Acad Sci U S A. 2018 09 25; 115(39):E9041-E9050.View Related Profiles.
Akiyama, Hisashi
Straub, John
Gummuluru, Suryaram
Keyes, Tom
PMID: 30190430.
Akiyama H, Miller CM, Ettinger CR, Belkina AC, Snyder-Cappione JE, Gummuluru S. HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction. Nat Commun. 2018 08 27; 9(1):3450.View Related Profiles.
Belkina, Anna
Miller, Caitlin
Akiyama, Hisashi
Snyder-Cappione , Jennifer
Gummuluru, Suryaram
PMID: 30150664.
Pena-Cruz V, Agosto LM, Akiyama H, Olson A, Moreau Y, Larrieux JR, Henderson A, Gummuluru S, Sagar M. HIV-1 replicates and persists in vaginal epithelial dendritic cells. J Clin Invest. 2018 Aug 01; 128(8):3439-3444.View Related Profiles.
Henderson, Andrew
Akiyama, Hisashi
Larrieux, Jean-Robert
Agosto Vazquez, Luis
Sagar, Manish
Gummuluru, Suryaram
PMID: 29723162.
Akiyama H, Ramirez NP, Gibson G, Kline C, Watkins S, Ambrose Z, Gummuluru S. Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon. J Virol. 2017 Nov 01; 91(21).View Related Profiles.
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 28794041.
Feizpour A, Stelter D, Wong C, Akiyama H, Gummuluru S, Keyes T, Reinhard BM. Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers. ACS Sens. 2017 Oct 27; 2(10):1415-1423.View Related Profiles.
Reinhard, Bjorn
Akiyama, Hisashi
Gummuluru, Suryaram
Keyes, Tom
PMID: 28933537.
Nazari M, Xi M, Lerch S, Alizadeh MH, Ettinger C, Akiyama H, Gillespie C, Gummuluru S, Erramilli S, Reinhard BM. Plasmonic Enhancement of Selective Photonic Virus Inactivation. Sci Rep. 2017 Sep 20; 7(1):11951.View Related Profiles.
Reinhard, Bjorn
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 28931903.
Ruedas JB, Ladner JT, Ettinger CR, Gummuluru S, Palacios G, Connor JH. Spontaneous Mutation at Amino Acid 544 of the Ebola Virus Glycoprotein Potentiates Virus Entry and Selection in Tissue Culture. J Virol. 2017 Aug 01; 91(15).View Related Profiles.
Connor, John
Gummuluru, Suryaram
PMID: 28539437.
Kozlowski E, Wasserman GA, Morgan M, O'Carroll D, Ramirez NP, Gummuluru S, Rah JY, Gower AC, Ieong M, Quinton LJ, Mizgerd JP, Jones MR. The RNA uridyltransferase Zcchc6 is expressed in macrophages and impacts innate immune responses. PLoS One. 2017; 12(6):e0179797.View Related Profiles.
Gower, Adam
Kozlowski, Elyse
Wasserman, Gregory
Mizgerd, Joseph
Quinton, Lee
Jones, Matthew
Ieong, Michael
Gummuluru, Suryaram
PMID: 28665939.
Miller CM, Akiyama H, Agosto LM, Emery A, Ettinger CR, Swanstrom RI, Henderson AJ, Gummuluru S. Virion-Associated Vpr Alleviates a Postintegration Block to HIV-1 Infection of Dendritic Cells. J Virol. 2017 Jul 01; 91(13).View Related Profiles.
Henderson, Andrew
Miller, Caitlin
Akiyama, Hisashi
Agosto Vazquez, Luis
Gummuluru, Suryaram
PMID: 28424288; DOI: 10.1128/JVI.00051-17;.
Ahi YS, Zhang S, Thappeta Y, Denman A, Feizpour A, Gummuluru S, Reinhard B, Muriaux D, Fivash MJ, Rein A. Functional Interplay Between Murine Leukemia Virus Glycogag, Serinc5, and Surface Glycoprotein Governs Virus Entry, with Opposite Effects on Gammaretroviral and Ebolavirus Glycoproteins. MBio. 2016 Nov 22; 7(6). PMID: 27879338; DOI: 10.1128/mBio.01985-16;.

Showing 10 of 43 results. Show More

Kijewski SD, Akiyama H, Feizpour A, Miller CM, Ramirez NG, Reinhard BM, Gummuluru S. Access of HIV-2 to CD169-dependent dendritic cell-mediated trans infection pathway is attenuated. Virology. 2016 Oct; 497:328-36.View Related Profiles.
Reinhard, Bjorn
Miller, Caitlin
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 27521724; PMCID: PMC5026622; DOI: 10.1016/j.virol.2016.07.029;.
Xu F, Reiser M, Yu X, Gummuluru S, Wetzler L, Reinhard BM. Lipid-Mediated Targeting with Membrane-Wrapped Nanoparticles in the Presence of Corona Formation. ACS Nano. 2016 Jan 26; 10(1):1189-200.View Related Profiles.
Reinhard, Bjorn
Wetzler, Lee
Reiser, Michael
Gummuluru, Suryaram
PMID: 26720275; PMCID: PMC4842014; DOI: 10.1021/acsnano.5b06501;.
Yu X, Xu F, Ramirez NG, Kijewski SD, Akiyama H, Gummuluru S, Reinhard BM. Dressing up Nanoparticles: A Membrane Wrap to Induce Formation of the Virological Synapse. ACS Nano. 2015; 9(4):4182-92.View Related Profiles.
Reinhard, Bjorn
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 25853367; PMCID: PMC4423798; DOI: 10.1021/acsnano.5b00415;.
Akiyama H, Ramirez NG, Gudheti MV, Gummuluru S. CD169-mediated trafficking of HIV to plasma membrane invaginations in dendritic cells attenuates efficacy of anti-gp120 broadly neutralizing antibodies. PLoS Pathog. 2015 Mar; 11(3):e1004751.View Related Profiles.
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 25760631; PMCID: PMC4356592; DOI: 10.1371/journal.ppat.1004751;.
Kijewski SD, Gummuluru S. A mechanistic overview of dendritic cell-mediated HIV-1 trans infection: the story so far. Future Virol. 2015 Mar; 10(3):257-269. PMID: 26213560.
Gummuluru S, Pina Ramirez NG, Akiyama H. CD169-dependent cell-associated HIV-1 transmission: a driver of virus dissemination. J Infect Dis. 2014 Dec 15; 210 Suppl 3:S641-7.View Related Profiles.
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 25414418; PMCID: PMC4303078; DOI: 10.1093/infdis/jiu442;.
Feizpour A, Yu X, Akiyama H, Miller CM, Edmans E, Gummuluru S, Reinhard BM. Quantifying lipid contents in enveloped virus particles with plasmonic nanoparticles. Small. 2015 Apr; 11(13):1592-602.View Related Profiles.
Reinhard, Bjorn
Miller, Caitlin
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 25382201; PMCID: PMC4447146; DOI: 10.1002/smll.201402184;.
Colvin TA, Gabai VL, Gong J, Calderwood SK, Li H, Gummuluru S, Matchuk ON, Smirnova SG, Orlova NV, Zamulaeva IA, Garcia-Marcos M, Li X, Young ZT, Rauch JN, Gestwicki JE, Takayama S, Sherman MY. Hsp70-Bag3 interactions regulate cancer-related signaling networks. Cancer Res. 2014 Sep 1; 74(17):4731-40.View Related Profiles.
Gong, Jianlin
Sherman, Michael
Garcia-Marcos, Mikel
Gummuluru, Suryaram
Colvin, Teresa
Gabai, Vladimir
PMID: 24994713; PMCID: PMC4174322; DOI: 10.1158/0008-5472.CAN-14-0747;.
Yu X, Feizpour A, Ramirez NG, Wu L, Akiyama H, Xu F, Gummuluru S, Reinhard BM. Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells. Nat Commun. 2014; 5:4136.View Related Profiles.
Reinhard, Bjorn
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 24947940; PMCID: PMC4109413; DOI: 10.1038/ncomms5136;.
Akiyama H, Miller C, Patel HV, Hatch SC, Archer J, Ramirez NG, Gummuluru S. Virus particle release from glycosphingolipid-enriched microdomains is essential for dendritic cell-mediated capture and transfer of HIV-1 and henipavirus. J Virol. 2014 Aug; 88(16):8813-25.View Related Profiles.
Miller, Caitlin
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 24872578; PMCID: PMC4136256; DOI: 10.1128/JVI.00992-14;.
Puryear WB, Akiyama H, Geer SD, Ramirez NP, Yu X, Reinhard BM, Gummuluru S. Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169. PLoS Pathog. 2013; 9(4):e1003291.View Related Profiles.
Reinhard, Bjorn
Akiyama, Hisashi
Gummuluru, Suryaram
Geer, Suzanne
Puryear, Wendy
PMID: 23593001; PMCID: PMC3623718; DOI: 10.1371/journal.ppat.1003291;.
Puryear WB, Gummuluru S. Role of glycosphingolipids in dendritic cell-mediated HIV-1 trans-infection. Adv Exp Med Biol. 2013; 762:131-53.View Related Profiles.
Gummuluru, Suryaram
Puryear, Wendy
PMID: 22975874; PMCID: PMC3686569; DOI: 10.1007/978-1-4614-4433-6_5;.
Schiralli Lester GM, Akiyama H, Evans E, Singh J, Gummuluru S, Henderson AJ. Interleukin 2-inducible T cell kinase (ITK) facilitates efficient egress of HIV-1 by coordinating Gag distribution and actin organization. Virology. 2013 Feb 5; 436(1):235-43.View Related Profiles.
Henderson, Andrew
Schiralli Lester, Gillian
Akiyama, Hisashi
Gummuluru, Suryaram
PMID: 23260110; PMCID: PMC3598624; DOI: 10.1016/j.virol.2012.11.015;.
Waheed AA, Brass AL, Gummuluru S, Tachedjian G. Host-pathogen interactions of retroviruses. Mol Biol Int. 2012; 2012:648512. PMID: 23150826; PMCID: PMC3488407; DOI: 10.1155/2012/648512;.
Puryear WB, Yu X, Ramirez NP, Reinhard BM, Gummuluru S. HIV-1 incorporation of host-cell-derived glycosphingolipid GM3 allows for capture by mature dendritic cells. Proc Natl Acad Sci U S A. 2012 May 8; 109(19):7475-80.View Related Profiles.
Reinhard, Bjorn
Gummuluru, Suryaram
Puryear, Wendy
PMID: 22529395; PMCID: PMC3358844; DOI: 10.1073/pnas.1201104109;.
Sagar M, Akiyama H, Etemad B, Ramirez N, Freitas I, Gummuluru S. Transmembrane domain membrane proximal external region but not surface unit-directed broadly neutralizing HIV-1 antibodies can restrict dendritic cell-mediated HIV-1 trans-infection. J Infect Dis. 2012 Apr 15; 205(8):1248-57.View Related Profiles.
Etemad, Behzad
Akiyama, Hisashi
Sagar, Manish
Gummuluru, Suryaram
PMID: 22396600; PMCID: PMC3308909; DOI: 10.1093/infdis/jis183;.
Hanley TM, Blay Puryear W, Gummuluru S, Viglianti GA. PPARgamma and LXR signaling inhibit dendritic cell-mediated HIV-1 capture and trans-infection. PLoS Pathog. 2010; 6:e1000981.View Related Profiles.
Viglianti, Gregory
Gummuluru, Suryaram
PMID: 20617179; PMCID: PMC2895661; DOI: 10.1371/journal.ppat.1000981;.
Hatch SC, Archer J, Gummuluru S. Glycosphingolipid composition of human immunodeficiency virus type 1 (HIV-1) particles is a crucial determinant for dendritic cell-mediated HIV-1 trans-infection. J Virol. 2009 Apr; 83(8):3496-506. PMID: 19193785; PMCID: PMC2663285; DOI: 10.1128/JVI.02249-08;.
Izquierdo-Useros N, Naranjo-Gómez M, Archer J, Hatch SC, Erkizia I, Blanco J, Borràs FE, Puertas MC, Connor JH, Fernández-Figueras MT, Moore L, Clotet B, Gummuluru S, Martinez-Picado J. Capture and transfer of HIV-1 particles by mature dendritic cells converges with the exosome-dissemination pathway. Blood. 2009 Mar 19; 113(12):2732-41.View Related Profiles.
Connor, John
Gummuluru, Suryaram
PMID: 18945959; PMCID: PMC2661860; DOI: 10.1182/blood-2008-05-158642;.
Nguyen DH, Gummuluru S, Hu J. Deamination-independent inhibition of hepatitis B virus reverse transcription by APOBEC3G. J Virol. 2007 May; 81(9):4465-72. PMID: 17314171; PMCID: PMC1900192.
Wiley RD, Gummuluru S. Immature dendritic cell-derived exosomes can mediate HIV-1 trans infection. Proc Natl Acad Sci U S A. 2006 Jan 17; 103(3):738-43. PMID: 16407131; PMCID: PMC1334656.
Gummuluru S, Rogel M, Stamatatos L, Emerman M. Binding of human immunodeficiency virus type 1 to immature dendritic cells can occur independently of DC-SIGN and mannose binding C-type lectin receptors via a cholesterol-dependent pathway. J Virol. 2003 Dec; 77(23):12865-74. PMID: 14610207; PMCID: PMC262553.
Gummuluru S, KewalRamani VN, Emerman M. Dendritic cell-mediated viral transfer to T cells is required for human immunodeficiency virus type 1 persistence in the face of rapid cell turnover. J Virol. 2002 Nov; 76(21):10692-701. PMID: 12368311; PMCID: PMC136613.
Gummuluru S, Emerman M. Advances in HIV molecular biology. AIDS. 2002; 16 Suppl 4:S17-23. PMID: 12698995.
Gummuluru S, Kinsey CM, Emerman M. An in vitro rapid-turnover assay for human immunodeficiency virus type 1 replication selects for cell-to-cell spread of virus. J Virol. 2000 Dec; 74(23):10882-91. PMID: 11069982; PMCID: PMC113167.
Gummuluru S, Emerman M. Cell cycle- and Vpr-mediated regulation of human immunodeficiency virus type 1 expression in primary and transformed T-cell lines. J Virol. 1999 Jul; 73(7):5422-30. PMID: 10364289; PMCID: PMC112598.
Whetter L, Novembre FJ, Saucier M, Gummuluru S, Dewhurst S. Costimulatory pathways in lymphocyte proliferation induced by the simian immunodeficiency virus SIVsmmPBj14. J Virol. 1998 Jul; 72(7):6155-8. PMID: 9621081; PMCID: PMC110423.
Krajewski S, James HJ, Ross J, Blumberg BM, Epstein LG, Gendelman HE, Gummuluru S, Dewhurst S, Sharer LR, Reed JC, Gelbard HA. Expression of pro- and anti-apoptosis gene products in brains from paediatric patients with HIV-1 encephalitis. Neuropathol Appl Neurobiol. 1997 Jun; 23(3):242-53. PMID: 9223134.
Gummuluru S, Novembre FJ, Lewis M, Gelbard HA, Dewhurst S. Apoptosis correlates with immune activation in intestinal lymphoid tissue from macaques acutely infected by a highly enteropathic simian immunodeficiency virus, SIVsmmPBj14. Virology. 1996 Nov 1; 225(1):21-32. PMID: 8918530.
Gummuluru S, Novembre FJ, Seshi B, Dewhurst S. SIVsmmPBj14 induces expression of a mucosal integrin on macaque lymphocytes. Virology. 1996 Jan 1; 215(1):97-100. PMID: 8553590.
van Loon NM, Gummuluru S, Sherwood DJ, Marentes R, Hall CB, Dewhurst S. Direct sequence analysis of human herpesvirus 6 (HHV-6) sequences from infants and comparison of HHV-6 sequences from mother/infant pairs. Clin Infect Dis. 1995 Oct; 21(4):1017-9. PMID: 8645791.
Talley AK, Dewhurst S, Perry SW, Dollard SC, Gummuluru S, Fine SM, New D, Epstein LG, Gendelman HE, Gelbard HA. Tumor necrosis factor alpha-induced apoptosis in human neuronal cells: protection by the antioxidant N-acetylcysteine and the genes bcl-2 and crmA. Mol Cell Biol. 1995 May; 15(5):2359-66. PMID: 7739519; PMCID: PMC230464.
Dollard SC, Gummuluru S, Tsang S, Fultz PN, Dewhurst S. Enhanced responsiveness to nuclear factor kappa B contributes to the unique phenotype of simian immunodeficiency virus variant SIVsmmPBj14. J Virol. 1994 Dec; 68(12):7800-9. PMID: 7966569; PMCID: PMC237241.

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 43 publications over 21 distinct years, with a maximum of 6 publications in 2017