Mikel Garcia-Marcos | Profiles RNS

Mikel Garcia-Marcos, PhD

Professor

Boston University Chobanian & Avedisian School of Medicine

Biochemistry & Cell Biology

PhD, University of the Basque Country
BS, University of the Basque Country

Websites

Biochemistry Faculty

ORCID : 0000-0001-9513-4826

We investigate signal transduction mechanisms with the ultimate goal of elucidating the molecular basis of human diseases and developing novel therapeutic approaches.

Our main interest is in heterotrimeric G proteins, which are molecular switches that relay extracellular signals. Heterotrimeric G proteins are primarily activated by G protein-coupled receptors (GPCRs) located at the plasma membrane. However, our lab focuses on a group of cytoplasmic factors that mimic the action of GPCRs by directly activating heterotrimeric G proteins. These non-receptor activators “rewire” signal transduction in different pathological settings, including cancer, fibrosis, insulin resistance and kidney failure, among many others.

Our current efforts are focused on:
(i) identifying the members of this group of non-receptor activators
(ii) defining their role in normal and pathological scenarios
(iii) characterizing the molecular mechanisms by which they are regulated
(iv) understanding the structural basis for their action on G proteins
(v) developing tools to specifically target them.

We are currently exploring this unconventional aspect of G protein signaling in the context of cancer, embryonic development and neurotransmission.Our multidisciplinary approach includes in vitro biochemistry to characterize protein complexes, cultured cells and model organisms (yeast, frog embryos, fish) for functional studies, and synthetic biology tools to manipulate and monitor signaling with exquisite spatiotemporal resolution..

Member
Boston University
BU-BMC Cancer Center

Member
Boston University
Evans Center for Interdisciplinary Biomedical Research

Member
Boston University
Genome Science Institute

BU Research
NIH RePORTER Research

Versatile and high-fidelity optical biosensor platforms for GPCR signaling
04/01/2023 - 01/31/2027 (PI)
NIH/National Institute of General Medical Sciences
5R01GM147931-02

Molecular evolution of synthetic probes for orphan GPCRs
01/01/2024 - 12/31/2026 (Key Person / Mentor)
NIH/National Heart, Lung, and Blood Institute
1F31HL172691-01

Alternative Mechanisms of Signaling via Trimeric G Proteins
06/01/2020 - 03/31/2025 (PI)
NIH/National Institute of Neurological Disorders & Stroke
5R01NS117101-10

Direct chemogenetic control of heterotrimeric G protein signaling
09/01/2022 - 08/31/2024 (PI)
NIH/National Institute of Neurological Disorders & Stroke
1R21NS127065-01A1

Non-canonical activation of heterotrimeric G protein signaling in vivo
09/20/2019 - 07/31/2024 (PI)
NIH/National Institute of General Medical Sciences
5R01GM136132-04

Student Intern Agreement with Universidad Francisco de Vitoria - Covadonga Lopez Ramos
02/01/2018 - 07/31/2024 (PI)
Universidad Francisco de Vitoria

Optical biosensor platforms for the direct interrogation of GPCR signaling in cardiovascular cells
01/01/2022 - 12/31/2023 (Key Person / Mentor)
American Heart Association

Targeting of non-canonical G protein signaling with small molecules
07/01/2018 - 06/30/2023 (PI)
NIH/National Institute of General Medical Sciences
5R01GM130120-04

Exploring the cancer mutational Landscape of G proteins with new biosensors
04/01/2019 - 11/01/2021 (Key Person / Mentor)
PI: Marcin Maziarz, PhD
American Cancer Society, Inc.

Next generation G protein activity biosensors
09/22/2018 - 08/31/2021 (PI)
NIH/National Institute of Mental Health
5R21MH118745-02

Showing 10 of 17 results. Show All Results

Title

Yr	Title	Project-Sub Proj
2024	Versatile and high-fidelity optical biosensor platforms for GPCR signaling	5R01GM147931-02
2024	Versatile and high-fidelity optical biosensor platforms for GPCR signaling	3R01GM147931-02S1
2024	Alternative Mechanisms of Signaling Via Trimeric G Proteins	5R01NS117101-10
2023	Versatile and high-fidelity optical biosensor platforms for GPCR signaling	1R01GM147931-01A1
2023	Alternative Mechanisms of Signaling Via Trimeric G Proteins	5R01NS117101-09
2022	Non-canonical activation of heterotrimeric G protein signaling in vivo	3R01GM136132-03S1
2022	Non-canonical activation of heterotrimeric G protein signaling in vivo	5R01GM136132-04
2022	Alternative mechanisms of signaling via trimeric G proteins	3R01NS117101-08S1
2022	Alternative mechanisms of signaling via trimeric G proteins	5R01NS117101-08
2021	Non-canonical activation of heterotrimeric G protein signaling in vivo	5R01GM136132-03

Showing 10 of 29 results. Show All Results

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis Copy PMIDs To Clipboard

Janicot R, Garcia-Marcos M. Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors. STAR Protoc. 2024 Dec 20; 5(4):103383. PMID: 39395175

Janicot R, Garcia-Marcos M. Protocol for detecting endogenous GPCR activity in primary cell cultures using ONE-GO biosensors. STAR Protoc. 2024 Dec 20; 5(4):103355. PMID: 39356642; PMCID: PMC11480228; DOI: 10.1016/j.xpro.2024.103355;

Luebbers A, Janicot R, Zhao J, Philibert CE, Garcia-Marcos M. Direct detection of endogenous Gai activity in cells with a sensitive conformational biosensor. bioRxiv. 2024 Aug 22.View Related Profiles.

Luebbers, Alex
Philibert, Clementine
Garcia-Marcos, Mikel

PMID: 39229046; PMCID: PMC11370452; DOI: 10.1101/2024.08.21.609006;

Janicot R, Garcia-Marcos M. Get Ready to Sharpen Your Tools: A Short Guide to Heterotrimeric G Protein Activity Biosensors. Mol Pharmacol. 2024 Aug 16; 106(3):129-144. PMID: 38991745; PMCID: PMC11331509; DOI: 10.1124/molpharm.124.000949;

Philibert CE, Garcia-Marcos M. Smooth operator(s): dialing up and down neurotransmitter responses by G-protein regulators. Trends Cell Biol. 2024 Jul 24.View Related Profiles.

Philibert, Clementine
Garcia-Marcos, Mikel

PMID: 39054106

Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M. Direct interrogation of context-dependent GPCR activity with a universal biosensor platform. Cell. 2024 Mar 14; 187(6):1527-1546.e25.View Related Profiles.

Luebbers, Alex
Philibert, Clementine
Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 38412860; PMCID: PMC10947893; DOI: 10.1016/j.cell.2024.01.028;

Garcia-Marcos M. Heterotrimeric G protein signaling without GPCRs: The Ga-binding-and-activating (GBA) motif. J Biol Chem. 2024 Mar; 300(3):105756. PMID: 38364891; PMCID: PMC10943482; DOI: 10.1016/j.jbc.2024.105756;

Cebul ER, Marivin A, Wexler LR, Perrat PN, Bénard CY, Garcia-Marcos M, Heiman MG. SAX-7/L1CAM acts with the adherens junction proteins MAGI-1, HMR-1/Cadherin, and AFD-1/Afadin to promote glial-mediated dendrite extension. bioRxiv. 2024 Jan 11. PMID: 38260503; PMCID: PMC10802611; DOI: 10.1101/2024.01.11.575259;

Janicot R, Maziarz M, Park JC, Luebbers A, Green E, Zhao J, Philibert C, Zhang H, Layne MD, Wu JC, Garcia-Marcos M. Direct interrogation of context-dependent GPCR activity with a universal biosensor platform. bioRxiv. 2024 Jan 02.View Related Profiles.

Luebbers, Alex
Philibert, Clementine
Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 38260348; PMCID: PMC10802303; DOI: 10.1101/2024.01.02.573921;

Lao-Peregrin C, Xiang G, Kim J, Srivastava I, Fall AB, Gerhard DM, Kohtala P, Kim D, Song M, Garcia-Marcos M, Levitz J, Lee FS. Synaptic plasticity via receptor tyrosine kinase/G-protein-coupled receptor crosstalk. Cell Rep. 2024 Jan 23; 43(1):113595. PMID: 38117654; PMCID: PMC10844890; DOI: 10.1016/j.celrep.2023.113595;

Showing 10 of 80 results. Show More

Luebbers A, Gonzalez-Hernandez AJ, Zhou M, Eyles SJ, Levitz J, Garcia-Marcos M. Dissecting the molecular basis for the modulation of neurotransmitter GPCR signaling by GINIP. Structure. 2024 Jan 04; 32(1):47-59.e7.View Related Profiles.

Luebbers, Alex
Garcia-Marcos, Mikel

PMID: 37989308; PMCID: PMC10872408; DOI: 10.1016/j.str.2023.10.010;

Lao-Peregrin C, Xiang G, Kim J, Srivastava I, Fall AB, Gerhard DM, Kohtala P, Kim D, Song M, Garcia-Marcos M, Levitz J, Lee FS. Synaptic plasticity via receptor tyrosine kinase/G protein-coupled receptor crosstalk. bioRxiv. 2023 Aug 28. PMID: 37693535; PMCID: PMC10491144; DOI: 10.1101/2023.08.28.555210;

Park JC, Luebbers A, Dao M, Semeano A, Nguyen AM, Papakonstantinou MP, Broselid S, Yano H, Martemyanov KA, Garcia-Marcos M. Fine-tuning GPCR-mediated neuromodulation by biasing signaling through different G protein subunits. Mol Cell. 2023 Jul 20; 83(14):2540-2558.e12.View Related Profiles.

Luebbers, Alex
Garcia-Marcos, Mikel

PMID: 37390816; PMCID: PMC10527995; DOI: 10.1016/j.molcel.2023.06.006;

Janicot R, Park JC, Garcia-Marcos M. Detecting GPCR Signals With Optical Biosensors of Ga-GTP in Cell Lines and Primary Cell Cultures. Curr Protoc. 2023 Jun; 3(6):e796. PMID: 37310083; PMCID: PMC10266833; DOI: 10.1002/cpz1.796;

Garcia-Marcos M. Stickier G-protein conformations. Nat Chem Biol. 2023 Jun; 19(6):665-666. PMID: 36646957

Garcia-Marcos M. Tails don't tell the whole story. Structure. 2023 May 04; 31(5):505-506. PMID: 37146571

Zhao J, DiGiacomo V, Ferreras-Gutierrez M, Dastjerdi S, Ibáñez de Opakua A, Park JC, Luebbers A, Chen Q, Beeler A, Blanco FJ, Garcia-Marcos M. Small-molecule targeting of GPCR-independent noncanonical G-protein signaling in cancer. Proc Natl Acad Sci U S A. 2023 May 02; 120(18):e2213140120.View Related Profiles.

Luebbers, Alex
Garcia-Marcos, Mikel

PMID: 37098067; PMCID: PMC10160980; DOI: 10.1073/pnas.2213140120;

Luebbers A, Zhou M, Eyles SJ, Garcia-Marcos M. Dissecting the molecular basis for the modulation of neurotransmitter GPCR signaling by GINIP. bioRxiv. 2023 Apr 21.View Related Profiles.

Luebbers, Alex
Garcia-Marcos, Mikel

PMID: 37131787; PMCID: PMC10153262; DOI: 10.1101/2023.04.20.537566;

Zhao J, DiGiacomo V, Ferreras-Gutierrez M, Dastjerdi S, de Opakua AI, Park JC, Luebbers A, Chen Q, Beeler A, Blanco FJ, Garcia-Marcos M. Small-molecule targeting of GPCR-independent non-canonical G protein signaling inhibits cancer progression. bioRxiv. 2023 Feb 19.View Related Profiles.

Luebbers, Alex
Garcia-Marcos, Mikel

PMID: 36824907; PMCID: PMC9949157; DOI: 10.1101/2023.02.18.529092;

Marivin A, Ho RX, Garcia-Marcos M. DAPLE orchestrates apical actomyosin assembly from junctional polarity complexes. J Cell Biol. 2022 May 02; 221(5).View Related Profiles.

Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 35389423; PMCID: PMC8996326; DOI: 10.1083/jcb.202111002;

Garcia-Marcos M. Complementary biosensors reveal different G-protein signaling modes triggered by GPCRs and non-receptor activators. Elife. 2021 03 31; 10. PMID: 33787494; PMCID: PMC8034979; DOI: 10.7554/eLife.65620;

Maziarz M, Federico A, Zhao J, Dujmusic L, Zhao Z, Monti S, Varelas X, Garcia-Marcos M. Naturally occurring hotspot cancer mutations in Ga13 promote oncogenic signaling. J Biol Chem. 2020 Dec 04; 295(49):16897-16904.View Related Profiles.

Zhao, Jingyi
Maziarz, Marcin
Garcia-Marcos, Mikel
Monti, Stefano
Varelas, Xaralabos

PMID: 33453920

Kalogriopoulos NA, Lopez-Sanchez I, Lin C, Ngo T, Midde KK, Roy S, Aznar N, Murray F, Garcia-Marcos M, Kufareva I, Ghassemian M, Ghosh P. Receptor tyrosine kinases activate heterotrimeric G proteins via phosphorylation within the interdomain cleft of Gai. Proc Natl Acad Sci U S A. 2020 11 17; 117(46):28763-28774. PMID: 33139573; PMCID: PMC7682395; DOI: 10.1073/pnas.2004699117;

Zhao, Jingyi
Maziarz, Marcin
Garcia-Marcos, Mikel
Monti, Stefano
Varelas, Xaralabos

PMID: 33109615; PMCID: PMC7864081; DOI: 10.1074/jbc.AC120.014698;

Garcia-Marcos M, Parag-Sharma K, Marivin A, Maziarz M, Luebbers A, Nguyen LT. Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein. Elife. 2020 09 16; 9.View Related Profiles.

Luebbers, Alex
Marivin, Arthur
Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 32936073; PMCID: PMC7515630; DOI: 10.7554/eLife.60155;

Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, Garcia-Marcos M. Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor Design. Cell. 2020 08 06; 182(3):770-785.e16.View Related Profiles.

Leyme, Anthony
Marivin, Arthur
Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 32634377; PMCID: PMC7415655; DOI: 10.1016/j.cell.2020.06.020;

Marivin A, Maziarz M, Zhao J, DiGiacomo V, Calvo IO, Mann EA, Ear J, Blanco-Canosa JB, Ross EM, Ghosh P, Garcia-Marcos M. Correction: DAPLE protein inhibits nucleotide exchange on Gas and Gaq via the same motif that activates Gai. J Biol Chem. 2020 Jun 26; 295(26):8867.View Related Profiles.

Marivin, Arthur
Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 32591443; PMCID: PMC7324496; DOI: 10.1074/jbc.AAC120.014344;

DiGiacomo V, Maziarz M, Luebbers A, Norris JM, Laksono P, Garcia-Marcos M. Probing the mutational landscape of regulators of G protein signaling proteins in cancer. Sci Signal. 2020 02 04; 13(617).View Related Profiles.

Luebbers, Alex
Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 32019900; PMCID: PMC7079226; DOI: 10.1126/scisignal.aax8620;

Marivin A, Maziarz M, Zhao J, DiGiacomo V, Olmos Calvo I, Mann EA, Ear J, Blanco-Canosa JB, Ross EM, Ghosh P, Garcia-Marcos M. DAPLE protein inhibits nucleotide exchange on Gas and Gaq via the same motif that activates Gai. J Biol Chem. 2020 02 21; 295(8):2270-2284.View Related Profiles.

Marivin, Arthur
Mann, Emily
Zhao, Jingyi
Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 31949046; PMCID: PMC7039542; DOI: 10.1074/jbc.RA119.011648;

Ghosh P, Garcia-Marcos M. Do All Roads Lead to Rome in G-Protein Activation? Trends Biochem Sci. 2020 03; 45(3):182-184. PMID: 31753703; PMCID: PMC7147754; DOI: 10.1016/j.tibs.2019.10.010;

Marivin A, Garcia-Marcos M. DAPLE and MPDZ bind to each other and cooperate to promote apical cell constriction. Mol Biol Cell. 2019 07 22; 30(16):1900-1910.View Related Profiles.

Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 31268831; PMCID: PMC6727773; DOI: 10.1091/mbc.E19-02-0091;

Marivin A, Morozova V, Walawalkar I, Leyme A, Kretov DA, Cifuentes D, Dominguez I, Garcia-Marcos M. GPCR-independent activation of G proteins promotes apical cell constriction in vivo. J Cell Biol. 2019 05 06; 218(5):1743-1763.View Related Profiles.

Leyme, Anthony
Marivin, Arthur
Cifuentes, Daniel
Kretov, Dmitry
Dominguez, Maria
Garcia-Marcos, Mikel

PMID: 30948426; PMCID: PMC6504902; DOI: 10.1083/jcb.201811174;

Maziarz M, Leyme A, Marivin A, Luebbers A, Patel PP, Chen Z, Sprang SR, Garcia-Marcos M. Atypical activation of the G protein Gaq by the oncogenic mutation Q209P. J Biol Chem. 2018 12 21; 293(51):19586-19599.View Related Profiles.

Luebbers, Alex
Leyme, Anthony
Marivin, Arthur
Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 30352874; PMCID: PMC6314142; DOI: 10.1074/jbc.RA118.005291;

Maziarz M, Broselid S, DiGiacomo V, Park JC, Luebbers A, Garcia-Navarrete L, Blanco-Canosa JB, Baillie GS, Garcia-Marcos M. A biochemical and genetic discovery pipeline identifies PLCd4b as a nonreceptor activator of heterotrimeric G-proteins. J Biol Chem. 2018 11 02; 293(44):16964-16983.View Related Profiles.

Luebbers, Alex
Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 30194280; PMCID: PMC6222092; DOI: 10.1074/jbc.RA118.003580;

Martemyanov KA, Garcia-Marcos M. Making useful gadgets with miniaturized G proteins. J Biol Chem. 2018 05 11; 293(19):7474-7475. PMID: 29752421; PMCID: PMC5949992; DOI: 10.1074/jbc.H118.002879;

Leyme A, Marivin A, Maziarz M, DiGiacomo V, Papakonstantinou MP, Patel PP, Blanco-Canosa JB, Walawalkar IA, Rodriguez-Davila G, Dominguez I, Garcia-Marcos M. Specific inhibition of GPCR-independent G protein signaling by a rationally engineered protein. Proc Natl Acad Sci U S A. 2017 11 28; 114(48):E10319-E10328.View Related Profiles.

Leyme, Anthony
Marivin, Arthur
Maziarz, Marcin
Dominguez, Maria
Garcia-Marcos, Mikel

PMID: 29133411; PMCID: PMC5715746; DOI: 10.1073/pnas.1707992114;

DiGiacomo V, Marivin A, Garcia-Marcos M. When Heterotrimeric G Proteins Are Not Activated by G Protein-Coupled Receptors: Structural Insights and Evolutionary Conservation. Biochemistry. 2018 01 23; 57(3):255-257.View Related Profiles.

Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 29035513; PMCID: PMC6082369; DOI: 10.1021/acs.biochem.7b00845;

Maziarz M, Garcia-Marcos M. Rapid kinetic BRET measurements to monitor G protein activation by GPCR and non-GPCR proteins. Methods Cell Biol. 2017; 142:145-157.View Related Profiles.

Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 28964333; PMCID: PMC5654623; DOI: 10.1016/bs.mcb.2017.08.001;

Maziarz M, Garcia-Marcos M. Fluorescence polarization assays to measure interactions between Ga subunits of heterotrimeric G proteins and regulatory motifs. Methods Cell Biol. 2017; 142:133-143.View Related Profiles.

Maziarz, Marcin
Garcia-Marcos, Mikel

PMID: 28964332; PMCID: PMC5654624; DOI: 10.1016/bs.mcb.2017.07.007;

DiGiacomo V, de Opakua AI, Papakonstantinou MP, Nguyen LT, Merino N, Blanco-Canosa JB, Blanco FJ, Garcia-Marcos M. The Gai-GIV binding interface is a druggable protein-protein interaction. Sci Rep. 2017 08 17; 7(1):8575. PMID: 28819150; PMCID: PMC5561080; DOI: 10.1038/s41598-017-08829-7;

de Opakua AI, Parag-Sharma K, DiGiacomo V, Merino N, Leyme A, Marivin A, Villate M, Nguyen LT, de la Cruz-Morcillo MA, Blanco-Canosa JB, Ramachandran S, Baillie GS, Cerione RA, Blanco FJ, Garcia-Marcos M. Molecular mechanism of Gai activation by non-GPCR proteins with a Ga-Binding and Activating motif. Nat Commun. 2017 05 18; 8:15163.View Related Profiles.

Leyme, Anthony
Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 28516903; PMCID: PMC5454376; DOI: 10.1038/ncomms15163;

Parag-Sharma K, Leyme A, DiGiacomo V, Marivin A, Broselid S, Garcia-Marcos M. Membrane Recruitment of the Non-receptor Protein GIV/Girdin (Ga-interacting, Vesicle-associated Protein/Girdin) Is Sufficient for Activating Heterotrimeric G Protein Signaling. J Biol Chem. 2016 Dec 30; 291(53):27098-27111.View Related Profiles.

Leyme, Anthony
Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 27864364; PMCID: PMC5207140; DOI: 10.1074/jbc.M116.764431;

Gupta V, Bhandari D, Leyme A, Aznar N, Midde KK, Lo IC, Ear J, Niesman I, López-Sánchez I, Blanco-Canosa JB, von Zastrow M, Garcia-Marcos M, Farquhar MG, Ghosh P. GIV/Girdin activates Gai and inhibits Gas via the same motif. Proc Natl Acad Sci U S A. 2016 09 27; 113(39):E5721-30.View Related Profiles.

Leyme, Anthony
Garcia-Marcos, Mikel

PMID: 27621449; PMCID: PMC5047194; DOI: 10.1073/pnas.1609502113;

Marivin A, Leyme A, Parag-Sharma K, DiGiacomo V, Cheung AY, Nguyen LT, Dominguez I, Garcia-Marcos M. Dominant-negative Ga subunits are a mechanism of dysregulated heterotrimeric G protein signaling in human disease. Sci Signal. 2016 Apr 12; 9(423):ra37.View Related Profiles.

Leyme, Anthony
Marivin, Arthur
Dominguez, Maria
Garcia-Marcos, Mikel

PMID: 27072656; PMCID: PMC4870087; DOI: 10.1126/scisignal.aad2429;

Garcia-Marcos M, VanHook AM. Science Signaling Podcast for 12 April 2016: G proteins in auriculo-condylar syndrome. Sci Signal. 2016; 9(423):pc9. PMID: 27072654; DOI: 10.1126/scisignal.aaf7740;

Leyme A, Marivin A, Garcia-Marcos M. GIV/Girdin (Ga-interacting, Vesicle-associated Protein/Girdin) Creates a Positive Feedback Loop That Potentiates Outside-in Integrin Signaling in Cancer Cells. J Biol Chem. 2016 Apr 8; 291(15):8269-82.View Related Profiles.

Leyme, Anthony
Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 26887938; PMCID: PMC4825026; DOI: 10.1074/jbc.M115.691550;

Coleman BD, Marivin A, Parag-Sharma K, DiGiacomo V, Kim S, Pepper JS, Casler J, Nguyen LT, Koelle MR, Garcia-Marcos M. Evolutionary Conservation of a GPCR-Independent Mechanism of Trimeric G Protein Activation. Mol Biol Evol. 2016 Mar; 33(3):820-37.View Related Profiles.

Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 26659249; PMCID: PMC4760084; DOI: 10.1093/molbev/msv336;

Leyme A, Marivin A, Perez-Gutierrez L, Nguyen LT, Garcia-Marcos M. Integrins activate trimeric G proteins via the nonreceptor protein GIV/Girdin. J Cell Biol. 2015 Sep 28; 210(7):1165-84.View Related Profiles.

Leyme, Anthony
Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 26391662; PMCID: PMC4586755; DOI: 10.1083/jcb.201506041;

Bhandari D, Lopez-Sanchez I, To A, Lo IC, Aznar N, Leyme A, Gupta V, Niesman I, Maddox AL, Garcia-Marcos M, Farquhar MG, Ghosh P. Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration-proliferation dichotomy. Proc Natl Acad Sci U S A. 2015 Sep 1; 112(35):E4874-83.View Related Profiles.

Leyme, Anthony
Garcia-Marcos, Mikel

PMID: 26286990; PMCID: PMC4568279; DOI: 10.1073/pnas.1514157112;

Aznar N, Midde KK, Dunkel Y, Lopez-Sanchez I, Pavlova Y, Marivin A, Barbazán J, Murray F, Nitsche U, Janssen KP, Willert K, Goel A, Abal M, Garcia-Marcos M, Ghosh P. Daple is a novel non-receptor GEF required for trimeric G protein activation in Wnt signaling. Elife. 2015; 4:e07091.View Related Profiles.

Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 26126266; PMCID: PMC4484057; DOI: 10.7554/eLife.07091;

Garcia-Marcos M, Ghosh P, Farquhar MG. GIV/Girdin transmits signals from multiple receptors by triggering trimeric G protein activation. J Biol Chem. 2015 Mar 13; 290(11):6697-704. PMID: 25605737; PMCID: PMC4358093; DOI: 10.1074/jbc.R114.613414;

Lin C, Ear J, Midde K, Lopez-Sanchez I, Aznar N, Garcia-Marcos M, Kufareva I, Abagyan R, Ghosh P. Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin. Mol Biol Cell. 2014 Nov 5; 25(22):3654-71. PMID: 25187647; PMCID: PMC4230624; DOI: 10.1091/mbc.E14-05-0978;

Colvin TA, Gabai VL, Gong J, Calderwood SK, Li H, Gummuluru S, Matchuk ON, Smirnova SG, Orlova NV, Zamulaeva IA, Garcia-Marcos M, Li X, Young ZT, Rauch JN, Gestwicki JE, Takayama S, Sherman MY. Hsp70-Bag3 interactions regulate cancer-related signaling networks. Cancer Res. 2014 Sep 1; 74(17):4731-40.View Related Profiles.

Gong, Jianlin
Sherman, Michael
Garcia-Marcos, Mikel
Gummuluru, Suryaram
Colvin, Teresa
Gabai, Vladimir

PMID: 24994713; PMCID: PMC4174322; DOI: 10.1158/0008-5472.CAN-14-0747;

Leyme A, Marivin A, Casler J, Nguyen LT, Garcia-Marcos M. Different biochemical properties explain why two equivalent Ga subunit mutants cause unrelated diseases. J Biol Chem. 2014 Aug 8; 289(32):21818-27.View Related Profiles.

Leyme, Anthony
Marivin, Arthur
Garcia-Marcos, Mikel

PMID: 24982418; PMCID: PMC4139202; DOI: 10.1074/jbc.M114.549790;

López-Sánchez I, Garcia-Marcos M, Mittal Y, Aznar N, Farquhar MG, Ghosh P. Protein kinase C-theta (PKC?) phosphorylates and inhibits the guanine exchange factor, GIV/Girdin. Proc Natl Acad Sci U S A. 2013 Apr 2; 110(14):5510-5. PMID: 23509302; PMCID: PMC3619294; DOI: 10.1073/pnas.1303392110;

Beas AO, Taupin V, Teodorof C, Nguyen LT, Garcia-Marcos M, Farquhar MG. Gas promotes EEA1 endosome maturation and shuts down proliferative signaling through interaction with GIV (Girdin). Mol Biol Cell. 2012 Dec; 23(23):4623-34. PMID: 23051738; PMCID: PMC3510023; DOI: 10.1091/mbc.E12-02-0133;

Klionsky DJ, Abdalla FC, Abeliovich H, Abraham RT, Acevedo-Arozena A, Adeli K, Agholme L, Agnello M, Agostinis P, Aguirre-Ghiso JA, Ahn HJ, Ait-Mohamed O, Ait-Si-Ali S, Akematsu T, Akira S, Al-Younes HM, Al-Zeer MA, Albert ML, Albin RL, Alegre-Abarrategui J, Aleo MF, Alirezaei M, Almasan A, Almonte-Becerril M, Amano A, Amaravadi R, Amarnath S, Amer AO, Andrieu-Abadie N, Anantharam V, Ann DK, Anoopkumar-Dukie S, Aoki H, Apostolova N, Arancia G, Aris JP, Asanuma K, Asare NY, Ashida H, Askanas V, Askew DS, Auberger P, Baba M, Backues SK, Baehrecke EH, Bahr BA, Bai XY, Bailly Y, Baiocchi R, Baldini G, Balduini W, Ballabio A, Bamber BA, Bampton ET, Bánhegyi G, Bartholomew CR, Bassham DC, Bast RC, Batoko H, Bay BH, Beau I, Béchet DM, Begley TJ, Behl C, Behrends C, Bekri S, Bellaire B, Bendall LJ, Benetti L, Berliocchi L, Bernardi H, Bernassola F, Besteiro S, Bhatia-Kissova I, Bi X, Biard-Piechaczyk M, Blum JS, Boise LH, Bonaldo P, Boone DL, Bornhauser BC, Bortoluci KR, Bossis I, Bost F, Bourquin JP, Boya P, Boyer-Guittaut M, Bozhkov PV, Brady NR, Brancolini C, Brech A, Brenman JE, Brennand A, Bresnick EH, Brest P, Bridges D, Bristol ML, Brookes PS, Brown EJ, Brumell JH, Brunetti-Pierri N, Brunk UT, Bulman DE, Bultman SJ, Bultynck G, Burbulla LF, Bursch W, Butchar JP, Buzgariu W, Bydlowski SP, Cadwell K, Cahová M, Cai D, Cai J, Cai Q, Calabretta B, Calvo-Garrido J, Camougrand N, Campanella M, Campos-Salinas J, Candi E, Cao L, Caplan AB, Carding SR, Cardoso SM, Carew JS, Carlin CR, Carmignac V, Carneiro LA, Carra S, Caruso RA, Casari G, Casas C, Castino R, Cebollero E, Cecconi F, Celli J, Chaachouay H, Chae HJ, Chai CY, Chan DC, Chan EY, Chang RC, Che CM, Chen CC, Chen GC, Chen GQ, Chen M, Chen Q, Chen SS, Chen W, Chen X, Chen X, Chen X, Chen YG, Chen Y, Chen Y, Chen YJ, Chen Z, Cheng A, Cheng CH, Cheng Y, Cheong H, Cheong JH, Cherry S, Chess-Williams R, Cheung ZH, Chevet E, Chiang HL, Chiarelli R, Chiba T, Chin LS, Chiou SH, Chisari FV, Cho CH, Cho DH, Choi AM, Choi D, Choi KS, Choi ME, Chouaib S, Choubey D, Choubey V, Chu CT, Chuang TH, Chueh SH, Chun T, Chwae YJ, Chye ML, Ciarcia R, Ciriolo MR, Clague MJ, Clark RS, Clarke PG, Clarke R, Codogno P, Coller HA, Colombo MI, Comincini S, Condello M, Condorelli F, Cookson MR, Coombs GH, Coppens I, Corbalan R, Cossart P, Costelli P, Costes S, Coto-Montes A, Couve E, Coxon FP, Cregg JM, Crespo JL, Cronjé MJ, Cuervo AM, Cullen JJ, Czaja MJ, D'Amelio M, Darfeuille-Michaud A, Davids LM, Davies FE, De Felici M, de Groot JF, de Haan CA, De Martino L, De Milito A, De Tata V, Debnath J, Degterev A, Dehay B, Delbridge LM, Demarchi F, Deng YZ, Dengjel J, Dent P, Denton D, Deretic V, Desai SD, Devenish RJ, Di Gioacchino M, Di Paolo G, Di Pietro C, Díaz-Araya G, Díaz-Laviada I, Diaz-Meco MT, Diaz-Nido J, Dikic I, Dinesh-Kumar SP, Ding WX, Distelhorst CW, Diwan A, Djavaheri-Mergny M, Dokudovskaya S, Dong Z, Dorsey FC, Dosenko V, Dowling JJ, Doxsey S, Dreux M, Drew ME, Duan Q, Duchosal MA, Duff K, Dugail I, Durbeej M, Duszenko M, Edelstein CL, Edinger AL, Egea G, Eichinger L, Eissa NT, Ekmekcioglu S, El-Deiry WS, Elazar Z, Elgendy M, Ellerby LM, Eng KE, Engelbrecht AM, Engelender S, Erenpreisa J, Escalante R, Esclatine A, Eskelinen EL, Espert L, Espina V, Fan H, Fan J, Fan QW, Fan Z, Fang S, Fang Y, Fanto M, Fanzani A, Farkas T, Farré JC, Faure M, Fechheimer M, Feng CG, Feng J, Feng Q, Feng Y, Fésüs L, Feuer R, Figueiredo-Pereira ME, Fimia GM, Fingar DC, Finkbeiner S, Finkel T, Finley KD, Fiorito F, Fisher EA, Fisher PB, Flajolet M, Florez-McClure ML, Florio S, Fon EA, Fornai F, Fortunato F, Fotedar R, Fowler DH, Fox HS, Franco R, Frankel LB, Fransen M, Fuentes JM, Fueyo J, Fujii J, Fujisaki K, Fujita E, Fukuda M, Furukawa RH, Gaestel M, Gailly P, Gajewska M, Galliot B, Galy V, Ganesh S, Ganetzky B, Ganley IG, Gao FB, Gao GF, Gao J, Garcia L, Garcia-Manero G, Garcia-Marcos M, Garmyn M, Gartel AL, Gatti E, Gautel M, Gawriluk TR, Gegg ME, Geng J, Germain M, Gestwicki JE, Gewirtz DA, Ghavami S, et al. GuidView Related Profiles.

Garcia-Marcos, Mikel
Ruiz-Opazo, Nelson
Shirihai, Orian
Wang, Zhi

PMID: 22966490; PMCID: PMC3404883; DOI: 10.4161/auto.19496;

Garcia-Marcos M, Kietrsunthorn PS, Pavlova Y, Adia MA, Ghosh P, Farquhar MG. Functional characterization of the guanine nucleotide exchange factor (GEF) motif of GIV protein reveals a threshold effect in signaling. Proc Natl Acad Sci U S A. 2012 Feb 7; 109(6):1961-6. PMID: 22308453; PMCID: PMC3277514; DOI: 10.1073/pnas.1120538109;

Lin C, Ear J, Pavlova Y, Mittal Y, Kufareva I, Ghassemian M, Abagyan R, Garcia-Marcos M, Ghosh P. Tyrosine phosphorylation of the Ga-interacting protein GIV promotes activation of phosphoinositide 3-kinase during cell migration. Sci Signal. 2011 Sep 27; 4(192):ra64. PMID: 21954290; PMCID: PMC4226136; DOI: 10.1126/scisignal.2002049;

Mittal Y, Pavlova Y, Garcia-Marcos M, Ghosh P. Src homology domain 2-containing protein-tyrosine phosphatase-1 (SHP-1) binds and dephosphorylates G(alpha)-interacting, vesicle-associated protein (GIV)/Girdin and attenuates the GIV-phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway. J Biol Chem. 2011 Sep 16; 286(37):32404-15. PMID: 21799016; PMCID: PMC3173146; DOI: 10.1074/jbc.M111.275685;

Garcia-Marcos M, Kietrsunthorn PS, Wang H, Ghosh P, Farquhar MG. G Protein binding sites on Calnuc (nucleobindin 1) and NUCB2 (nucleobindin 2) define a new class of G(alpha)i-regulatory motifs. J Biol Chem. 2011 Aug 12; 286(32):28138-49. PMID: 21653697; PMCID: PMC3151059; DOI: 10.1074/jbc.M110.204099;

Ghosh P, Garcia-Marcos M, Farquhar MG. GIV/Girdin is a rheostat that fine-tunes growth factor signals during tumor progression. Cell Adh Migr. 2011 May-Jun; 5(3):237-48. PMID: 21546796; PMCID: PMC3210208; DOI: 10.4161/cam.5.3.15909;

Garcia-Marcos M, Ghosh P, Farquhar MG. Molecular basis of a novel oncogenic mutation in GNAO1. Oncogene. 2011 Jun 9; 30(23):2691-6. PMID: 21317923; PMCID: PMC3349449; DOI: 10.1038/onc.2010.645;

Garcia-Marcos M, Ear J, Farquhar MG, Ghosh P. A GDI (AGS3) and a GEF (GIV) regulate autophagy by balancing G protein activity and growth factor signals. Mol Biol Cell. 2011 Mar 1; 22(5):673-86. PMID: 21209316; PMCID: PMC3046063; DOI: 10.1091/mbc.E10-08-0738;

Garcia-Marcos M, Jung BH, Ear J, Cabrera B, Carethers JM, Ghosh P. Expression of GIV/Girdin, a metastasis-related protein, predicts patient survival in colon cancer. FASEB J. 2011 Feb; 25(2):590-9. PMID: 20974669; PMCID: PMC3023389; DOI: 10.1096/fj.10-167304;

Fontanils U, Seil M, Pochet S, El Ouaaliti M, Garcia-Marcos M, Dehaye JP, Marino A. Stimulation by P2X7 receptors of calcium-dependent production of reactive oxygen species (ROS) in rat submandibular glands. Biochim Biophys Acta. 2010 Nov; 1800(11):1183-91. PMID: 20655985; DOI: 10.1016/j.bbagen.2010.07.007;

Ghosh P, Beas AO, Bornheimer SJ, Garcia-Marcos M, Forry EP, Johannson C, Ear J, Jung BH, Cabrera B, Carethers JM, Farquhar MG. A G{alpha}i-GIV molecular complex binds epidermal growth factor receptor and determines whether cells migrate or proliferate. Mol Biol Cell. 2010 Jul 1; 21(13):2338-54. PMID: 20462955; PMCID: PMC2893996; DOI: 10.1091/mbc.E10-01-0028;

Garcia-Marcos M, Ghosh P, Ear J, Farquhar MG. A structural determinant that renders G alpha(i) sensitive to activation by GIV/girdin is required to promote cell migration. J Biol Chem. 2010 Apr 23; 285(17):12765-77. PMID: 20157114; PMCID: PMC2857069; DOI: 10.1074/jbc.M109.045161;

Garcia-Marcos M, Ghosh P, Farquhar MG. GIV is a nonreceptor GEF for G alpha i with a unique motif that regulates Akt signaling. Proc Natl Acad Sci U S A. 2009 Mar 3; 106(9):3178-83. PMID: 19211784; PMCID: PMC2651282; DOI: 10.1073/pnas.0900294106;

Garcia-Marcos M, Dehaye JP, Marino A. Membrane compartments and purinergic signalling: the role of plasma membrane microdomains in the modulation of P2XR-mediated signalling. FEBS J. 2009 Jan; 276(2):330-40. PMID: 19076211; DOI: 10.1111/j.1742-4658.2008.06794.x;

Ghosh P, Garcia-Marcos M, Bornheimer SJ, Farquhar MG. Activation of Galphai3 triggers cell migration via regulation of GIV. J Cell Biol. 2008 Jul 28; 182(2):381-93. PMID: 18663145; PMCID: PMC2483528; DOI: 10.1083/jcb.200712066;

Seil M, Fontanils U, Etxebarria IG, Pochet S, Garcia-Marcos M, Marino A, Dehaye JP. Pharmacological evidence for the stimulation of NADPH oxidase by P2X(7) receptors in mouse submandibular glands. Purinergic Signal. 2008 Dec; 4(4):347-55. PMID: 18581262; PMCID: PMC2583204; DOI: 10.1007/s11302-008-9118-y;

Pochet S, Garcia-Marcos M, Seil M, Otto A, Marino A, Dehaye JP. Contribution of two ionotropic purinergic receptors to ATP responses in submandibular gland ductal cells. Cell Signal. 2007 Oct; 19(10):2155-64. PMID: 17651941

Garcia-Marcos M, Pochet S, Marino A, Dehaye JP. P2X7 and phospholipid signalling: the search of the "missing link" in epithelial cells. Cell Signal. 2006 Dec; 18(12):2098-104. PMID: 16815675

García-Marcos M, Pochet S, Tandel S, Fontanils U, Astigarraga E, Fernández-González JA, Kumps A, Marino A, Dehaye JP. Characterization and comparison of raft-like membranes isolated by two different methods from rat submandibular gland cells. Biochim Biophys Acta. 2006 Jun; 1758(6):796-806. PMID: 16842738; DOI: 10.1016/j.bbamem.2006.05.008;

Pochet S, Tandel S, Querriére S, Tre-Hardy M, Garcia-Marcos M, De Lorenzi M, Vandenbranden M, Marino A, Devleeschouwer M, Dehaye JP. Modulation by LL-37 of the responses of salivary glands to purinergic agonists. Mol Pharmacol. 2006 Jun; 69(6):2037-46. PMID: 16514052

Garcia-Marcos M, Pérez-Andrés E, Tandel S, Fontanils U, Kumps A, Kabré E, Gómez-Muñoz A, Marino A, Dehaye JP, Pochet S. Coupling of two pools of P2X7 receptors to distinct intracellular signaling pathways in rat submandibular gland. J Lipid Res. 2006 Apr; 47(4):705-14. PMID: 16415476

Garcia-Marcos M, Fontanils U, Aguirre A, Pochet S, Dehaye JP, Marino A. Role of sodium in mitochondrial membrane depolarization induced by P2X7 receptor activation in submandibular glands. FEBS Lett. 2005 Oct 10; 579(24):5407-13. PMID: 16198349; DOI: 10.1016/j.febslet.2005.08.074;

Garcia-Marcos M, Tandel S, Pochet S, Genin J, De Lorenzi M, Gomez F, Kumps A, Marino A, Dehaye JP. Cholesterol depletion perturbs calcium handling by rat submandibular glands. J Cell Physiol. 2005 May; 203(2):429-38. PMID: 15521067; DOI: 10.1002/jcp.20241;

Pochet S, García-Marcos M, Fernandez M, Marino A, Dehaye JP. Regulation by clozapine of calcium handling by rat submandibular acinar cells. Cell Calcium. 2003 Dec; 34(6):465-75. PMID: 14572805

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 80 publications over 21 distinct years, with a maximum of 10 publications in 2023