Keywords
Last Name

Daniel Cifuentes, PhD

TitleAssistant Professor
InstitutionBoston University School of Medicine
DepartmentBiochemistry
Address72 E. Concord Street, K6
Boston MA 02118
ORCID ORCID Icon0000-0001-5442-4843
 Awards and Honors

Start-EndDescription
2016-2019Boston University: Peter Paul Career Development Professorship
 Research Expertise & Professional Interests
The beginning of a new life is one of the most enigmatic and dynamic events in Biology. The long term goal of my laboratory is to understand how post-transcriptional regulation of RNA and proteins orchestrates development of a single cell oocyte into a whole embryo and how these mechanisms can be extrapolated to other biological transitions such as cellular reprogramming or malignant transformation.

To address these questions, my laboratory focuses on the mechanisms of post-transcriptional regulation during vertebrate embryogenesis using zebrafish as a model system. This approach allows us to dissect the process of reprogramming in vivo within the context of a whole organism. The combination of fish embryo microinjection together with powerful genetic, genomic, and proteomic approaches provides a unique platform to address these central questions in biology.

 Self-Described Keywords
  • zebrafish
  • CRISPR
  • microRNA
  • iCLIP
  • microinjection
 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Vejnar CE, Moreno-Mateos MA, Cifuentes D, Bazzini AA, Giraldez AJ. Optimized CRISPR-Cas9 System for Genome Editing in Zebrafish. Cold Spring Harb Protoc. 2016 Oct 03; 2016(10):pdb.prot086850. PMID: 27698232.
    View in: PubMed
  2. Vejnar CE, Moreno-Mateos MA, Cifuentes D, Bazzini AA, Giraldez AJ. Optimization Strategies for the CRISPR-Cas9 Genome-Editing System. Cold Spring Harb Protoc. 2016 Oct 03; 2016(10):pdb.top090894. PMID: 27698246.
    View in: PubMed
  3. Hoffman EJ, Turner KJ, Fernandez JM, Cifuentes D, Ghosh M, Ijaz S, Jain RA, Kubo F, Bill BR, Baier H, Granato M, Barresi MJ, Wilson SW, Rihel J, State MW, Giraldez AJ. Estrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2. Neuron. 2016 Feb 17; 89(4):725-33. PMID: 26833134.
    View in: PubMed
  4. Yoda M, Cifuentes D, Izumi N, Sakaguchi Y, Suzuki T, Giraldez AJ, Tomari Y. Poly(A)-specific ribonuclease mediates 3'-end trimming of Argonaute2-cleaved precursor microRNAs. Cell Rep. 2013 Nov 14; 5(3):715-26. PMID: 24209750.
    View in: PubMed
  5. Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, Wolfe SA. Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct; 138(20):4555-64. PMID: 21937602.
    View in: PubMed
  6. Sander JD, Dahlborg EJ, Goodwin MJ, Cade L, Zhang F, Cifuentes D, Curtin SJ, Blackburn JS, Thibodeau-Beganny S, Qi Y, Pierick CJ, Hoffman E, Maeder ML, Khayter C, Reyon D, Dobbs D, Langenau DM, Stupar RM, Giraldez AJ, Voytas DF, Peterson RT, Yeh JR, Joung JK. Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA). Nat Methods. 2011 Jan; 8(1):67-9. PMID: 21151135.
    View in: PubMed
  7. Cifuentes D, Xue H, Taylor DW, Patnode H, Mishima Y, Cheloufi S, Ma E, Mane S, Hannon GJ, Lawson ND, Wolfe SA, Giraldez AJ. A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity. Science. 2010 Jun 25; 328(5986):1694-8. PMID: 20448148.
    View in: PubMed
  8. Pescador N, Villar D, Cifuentes D, Garcia-Rocha M, Ortiz-Barahona A, Vazquez S, Ordoñez A, Cuevas Y, Saez-Morales D, Garcia-Bermejo ML, Landazuri MO, Guinovart J, del Peso L. Hypoxia promotes glycogen accumulation through hypoxia inducible factor (HIF)-mediated induction of glycogen synthase 1. PLoS One. 2010; 5(3):e9644. PMID: 20300197.
    View in: PubMed
  9. Flores E, Cifuentes D, Fernández-Novell JM, Medrano A, Bonet S, Briz MD, Pinart E, Peña A, Rigau T, Rodríguez-Gil JE. Freeze-thawing induces alterations in the protamine-1/DNA overall structure in boar sperm. Theriogenology. 2008 Jun; 69(9):1083-94. PMID: 18359506.
    View in: PubMed
  10. Cifuentes D, Martínez-Pons C, García-Rocha M, Galina A, Ribas de Pouplana L, Guinovart JJ. Hepatic glycogen synthesis in the absence of glucokinase: the case of embryonic liver. J Biol Chem. 2008 Feb 29; 283(9):5642-9. PMID: 18165236.
    View in: PubMed
  11. Vilchez D, Ros S, Cifuentes D, Pujadas L, Vallès J, García-Fojeda B, Criado-García O, Fernández-Sánchez E, Medraño-Fernández I, Domínguez J, García-Rocha M, Soriano E, Rodríguez de Córdoba S, Guinovart JJ. Mechanism suppressing glycogen synthesis in neurons and its demise in progressive myoclonus epilepsy. Nat Neurosci. 2007 Nov; 10(11):1407-13. PMID: 17952067.
    View in: PubMed
  12. Rezende GL, Logullo C, Meyer L, Machado LB, Oliveira-Carvalho AL, Zingali RB, Cifuentes D, Galina A. Partial purification of tightly bound mitochondrial hexokinase from maize (Zea mays L.) root membranes. Braz J Med Biol Res. 2006 Sep; 39(9):1159-69. PMID: 16981044.
    View in: PubMed
  13. Cid E, Cifuentes D, Baqué S, Ferrer JC, Guinovart JJ. Determinants of the nucleocytoplasmic shuttling of muscle glycogen synthase. FEBS J. 2005 Jun; 272(12):3197-213. PMID: 15955076.
    View in: PubMed
  14. Guil S, de La Iglesia N, Fernández-Larrea J, Cifuentes D, Ferrer JC, Guinovart JJ, Bach-Elias M. Alternative splicing of the human proto-oncogene c-H-ras renders a new Ras family protein that trafficks to cytoplasm and nucleus. Cancer Res. 2003 Sep 1; 63(17):5178-87. PMID: 14500341.
    View in: PubMed
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