Daniel Cifuentes, PhD
Assistant Professor
Boston University School of Medicine
Dept of Biochemistry

PhD, Universidad de Barcelona




The beginning of a new life is one of the most enigmatic and dynamic events in Biology. The long term goal of my laboratory is to understand how post-transcriptional regulation of RNA and proteins orchestrates development of a single cell oocyte into a whole embryo and how these mechanisms can be extrapolated to other biological transitions such as cellular reprogramming or malignant transformation.

To address these questions, my laboratory focuses on the mechanisms of post-transcriptional regulation during vertebrate embryogenesis using zebrafish as a model system. This approach allows us to dissect the process of reprogramming in vivo within the context of a whole organism. The combination of fish embryo microinjection together with powerful genetic, genomic, and proteomic approaches provides a unique platform to address these central questions in biology.

Member
Boston University
Genome Science Institute



2016-2019 Boston University: Peter Paul Career Development Professorship


Analysis of non-canonical functions of microRNAs
03/01/2019 - 02/29/2024 (PI)
NIH/National Institute of General Medical Sciences
1R01GM130935-01

Deep characterization of the role biogenesis and function of Ebola virus microRNAs
07/01/2019 - 06/30/2021 (Multi-PI)
PI: Daniel Cifuentes, PhD
NIH/National Institute of Allergy & Infectious Diseases
1R21AI147285-01

Boston University RNA Club
11/01/2019 - 10/31/2020 (PI)
RNA Society


Boston University School of Medicine RNA Club
11/01/2018 - 10/31/2019 (PI)
RNA Society


Analysis of the Molecular Machinery of Micro RNA Processing Pathways
04/08/2016 - 03/31/2019 (PI)
NIH/National Institute of Child Health & Human Development
5R00HD071968-05

Boston University School of Medicine RNA Club
11/01/2017 - 10/31/2018 (PI)
RNA Society


Student Intern Agreement with Unviersidad Francisco de Vitoria-Pablo Perez Larrubia
02/01/2018 - 07/31/2018 (PI)
Universidad Francisco de Vitoria


Student Intern Agreement with Universidad Francisco de Vitoria - Covadonga Lopez Ramos
02/01/2018 - 07/31/2018 (Co-PI)
PI: Mikel Garcia-Marcos, PhD
Universidad Francisco de Vitoria


RNA Salon at Boston University
11/01/2016 - 10/31/2017 (PI)
RNA Society




Title


Yr Title Project-Sub Proj Pubs
2020 Developing a high-throughput method to validate microRNA biogenesis in vivo. 1R21GM138951-01
2020 Deep characterization of the biogenesis and function of Ebola virus microRNAs 5R21AI147285-02
2020 Analysis of non-canonical functions of microRNAs 5R01GM130935-02
2020 Analysis of non-canonical functions of microRNAs 5R01GM130935-02
2020 Analysis of non-canonical functions of microRNAs 3R01GM130935-02S1
2019 Deep characterization of the biogenesis and function of Ebola virus microRNAs 1R21AI147285-01
2019 Analysis of non-canonical functions of microRNAs 1R01GM130935-01
2018 Analysis of the Molecular Machinery of microRNA-processing pathways 5R00HD071968-05 3
2017 Analysis of the Molecular Machinery of microRNA-processing pathways 5R00HD071968-04 3
2016 Analysis of the Molecular Machinery of microRNA-processing pathways 4R00HD071968-03 3
Showing 10 of 12 results. Show All Results

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis       Copy PMIDs To Clipboard

  1. Kretov DA, Walawalkar IA, Mora-Martin A, Shafik AM, Moxon S, Cifuentes D. Ago2-Dependent Processing Allows miR-451 to Evade the Global MicroRNA Turnover Elicited during Erythropoiesis. Mol Cell. 2020 04 16; 78(2):317-328.e6.View Related Profiles. PMID: 32191872
     
  2. Vejnar CE, Abdel Messih M, Takacs CM, Yartseva V, Oikonomou P, Christiano R, Stoeckius M, Lau S, Lee MT, Beaudoin JD, Musaev D, Darwich-Codore H, Walther TC, Tavazoie S, Cifuentes D, Giraldez AJ. Genome wide analysis of 3' UTR sequence elements and proteins regulating mRNA stability during maternal-to-zygotic transition in zebrafish. Genome Res. 2019 07; 29(7):1100-1114. PMID: 31227602
     
  3. Marivin A, Morozova V, Walawalkar I, Leyme A, Kretov DA, Cifuentes D, Dominguez I, Garcia-Marcos M. GPCR-independent activation of G proteins promotes apical cell constriction in vivo. J Cell Biol. 2019 05 06; 218(5):1743-1763.View Related Profiles. PMID: 30948426
     
  4. Kumaradevan S, Lee SY, Richards S, Lyle C, Zhao Q, Tapan U, Jiangliu Y, Ghumman S, Walker J, Belghasem M, Arinze N, Kuhnen A, Weinberg J, Francis J, Hartshorn K, Kolachalama VB, Cifuentes D, Rahimi N, Chitalia VC. c-Cbl Expression Correlates with Human Colorectal Cancer Survival and Its Wnt/ß-Catenin Suppressor Function Is Regulated by Tyr371 Phosphorylation. Am J Pathol. 2018 08; 188(8):1921-1933.View Related Profiles. PMID: 30029779; DOI: 10.1016/j.ajpath.2018.05.007;
     
  5. Kretov DA, Shafik AM, Cifuentes D. Assessing miR-451 Activity and Its Role in Erythropoiesis. Methods Mol Biol. 2018; 1680:179-190.View Related Profiles. PMID: 29030849
     
  6. Vejnar CE, Moreno-Mateos MA, Cifuentes D, Bazzini AA, Giraldez AJ. Optimized CRISPR-Cas9 System for Genome Editing in Zebrafish. Cold Spring Harb Protoc. 2016 Oct 03; 2016(10):pdb.prot086850. PMID: 27698232; DOI: 10.1101/pdb.prot086850;
     
  7. Vejnar CE, Moreno-Mateos MA, Cifuentes D, Bazzini AA, Giraldez AJ. Optimization Strategies for the CRISPR-Cas9 Genome-Editing System. Cold Spring Harb Protoc. 2016 Oct 03; 2016(10):pdb.top090894. PMID: 27698246; DOI: 10.1101/pdb.top090894;
     
  8. Hoffman EJ, Turner KJ, Fernandez JM, Cifuentes D, Ghosh M, Ijaz S, Jain RA, Kubo F, Bill BR, Baier H, Granato M, Barresi MJ, Wilson SW, Rihel J, State MW, Giraldez AJ. Estrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2. Neuron. 2016 Feb 17; 89(4):725-33. PMID: 26833134; PMCID: PMC4766582; DOI: 10.1016/j.neuron.2015.12.039;
     
  9. Yoda M, Cifuentes D, Izumi N, Sakaguchi Y, Suzuki T, Giraldez AJ, Tomari Y. Poly(A)-specific ribonuclease mediates 3'-end trimming of Argonaute2-cleaved precursor microRNAs. Cell Rep. 2013 Nov 14; 5(3):715-26. PMID: 24209750; PMCID: PMC3856240; DOI: 10.1016/j.celrep.2013.09.029;
     
  10. Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, Wolfe SA. Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct; 138(20):4555-64. PMID: 21937602; PMCID: PMC3177320; DOI: 10.1242/dev.066779;
     
Showing 10 of 19 results. Show More

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 19 publications over 12 distinct years, with a maximum of 3 publications in 2010 and 2016

YearPublications
20031
20051
20061
20072
20081
20103
20111
20131
20163
20182
20192
20201

In addition to these self-described keywords below, a list of MeSH based concepts is available here.

CRISPR
iCLIP
microinjection
microRNA
zebrafish
Contact for Mentoring:

72 E. Concord St Silvio Conte (K)
Boston MA 02118
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