Joseph Zaia, PhD
Professor
Boston University School of Medicine
Dept of Biochemistry

PhD, Massachusetts Institute of Technology



The manner in which a cell responds to many growth factor stimuli depends on interactions between glycosaminoglycans (GAGs), growth factors, and growth factor receptors. Extracellular matrix GAGs binds growth factors, creating morphogens gradients essential to tissue patterning. Because these events depend on the fine structure of the GAG chains present, regulation of GAG biosynthesis is a key factor for understanding normal and disease related cellular growth
The key to exploiting an understanding of GAG structure-function relationships for human disease therapy is to winnow oligosaccharide-protein binding patterns from heterogeneous biological preparations. Toward this end, we have developed mass spectral methods for GAGs that enable comparison of structures as a function of biological variables.

The long term research aims are (1) to develop a fundamental understanding of the manner in which glycosaminoglycan expression is varied according to the cellular growth environment related to human disease and (2) to identify HS chain structures useful as therapeutic targets.

New bioinformatics methods are essential to realizing these goals. The data produced using our methods are information rich and not amenable to manual interpretation. Further, the methods needed are distinct from those used in genomics and proteomics. We are developing bioinformatics methods appropriate for interpretation of structural data on glycosaminoglycans and other carbohydrates to identify targets for disease therapy.

Graduate Faculty (Primary Mentor of Grad Students)
Boston University School of Medicine, Division of Graduate Medical Sciences


Member
Boston University
Bioinformatics Graduate Program


Center Faculty Member
Boston University School of Medicine
Mass Spectrometry




Glycomics and proteomics of brain specimens
03/15/2018 - 12/31/2022 (PI)
The Scripps Research Institute NIH NIDA
1R01DA046170-01

An open-source software suite for processing glycomics and glycoproteomics mass spectral data
08/14/2017 - 07/31/2020 (PI)
NIH/National Cancer Institute
1U01CA221234-01

Proteomic and glycomic analysis of human brain and lung cancers
07/01/2016 - 06/30/2018 (PI)
The Regents of the University of California, San Francisco NIH NCI
4U01CA168878-05

Software for Automated Interpretation of Heparan Sulfate Tandem Mass Spectra
09/15/2015 - 06/30/2018 (PI)
NIH/National Heart, Lung, and Blood Institute
3R21HL131554-02S1

Thalamic Axonal Pathways and Extracellular Matrix Abnormalities in Schizophrenia
09/01/2015 - 06/30/2018 (PI)
McLean Hospital Corporation NIH NIMH
5R01MH105608-03

Quantitative Profiling of Glycosaminoglycans from Breast Tumor Tissue Arrays
07/09/2014 - 06/30/2018 (PI)
NIH/National Cancer Institute
5R21CA177479-03

Viral Vector Transduction Deficiencies in the Aged Nigrostriatal System
09/30/2016 - 06/30/2017 (PI)
Michigan State University NIH NIA
1R56AG052328-01A1

Quantitative glycomics and proteomics combined with laser capture microdissection
06/01/2013 - 05/21/2017 (PI)
Agilent Technologies, Inc.


A Thermo-Fisher Scientific Q-Exactive HF Mass Spectrometry System
05/01/2016 - 04/30/2017 (PI)
NIH/Office of the Director
1S10OD021651-01

Extracellular Sulfatases As Novel Biomarkers for Early Detection of Cancer
07/01/2015 - 06/30/2016 (PI)
The Regents of the University of California, San Francisco NIH NCI
5U01CA168878-04

Showing 10 of 19 results. Show All Results



Yr Title Project-Sub Proj Pubs
2017 An open-source software suite for processing glycomics and glycoproteomics mass spectral data 1U01CA221234-01
2016 A Thermo-Fisher Scientific Q-Exactive HF Mass Spectrometry System 1S10OD021651-01 1
2016 Software for automated interpretation of heparan sulfate tandem mass spectra 5R21HL131554-02 2
2016 Software for automated interpretation of heparan sulfate tandem mass spectra 3R21HL131554-02S1 2
2016 Quantitative profiling of glycosaminoglycans from breast tumor tissue arrays 5R21CA177479-03 1
2015 Software for automated interpretation of heparan sulfate tandem mass spectra 1R21HL131554-01 2
2014 Quantitative profiling of glycosaminoglycans from breast tumor tissue arrays 1R21CA177479-01A1 1
2014 Quantitative profiling of glycosaminoglycans from breast tumor tissue arrays 1R21CA177479-01A1 1
2013 Post-biosynthetic Remodeling of Heparan Sulfate 5R01HL098950-04 27
2012 Post-biosynthetic Remodeling of Heparan Sulfate 5R01HL098950-03 27
Showing 10 of 130 results. Show All Results
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

  1. Raghunathan R, Polinski NK, Klein JA, Hogan JD, Shao C, Khatri K, Leon DR, McComb ME, Manfredsson FP, Sortwell CE, Zaia J. Glycomic and Proteomic changes in aging brain nigrostriatal pathway. Mol Cell Proteomics. 2018 Jun 18.View Related Profiles. PMID: 29915149; DOI: 10.1074/mcp.RA118.000680;.
     
  2. Zaia J, Bierbaum VM. Focus on Mass Spectrometry in Glycobiology and Related Fields, Honoring Catherine E. Costello, Recipient of the 2017 ASMS Award for a Distinguished Contribution in Mass Spectrometry. J Am Soc Mass Spectrom. 2018 Jun; 29(6):1061-1064. PMID: 29855889.
     
  3. Klein J, Carvalho L, Zaia J. Application of Network Smoothing to Glycan LC-MS Profiling. Bioinformatics. 2018 May 22. PMID: 29790907.
     
  4. Klein JA, Meng L, Zaia J. Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides. Mol Cell Proteomics. 2018 Aug; 17(8):1578-1590. PMID: 29773674.
     
  5. van Eijk M, Rynkiewicz MJ, Khatri K, Leymarie N, Zaia J, White MR, Hartshorn KL, Cafarella TR, van Die I, Hessing M, Seaton BA, Haagsman HP. Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus. J Biol Chem. 2018 Jul 06; 293(27):10646-10662.View Related Profiles. PMID: 29769321.
     
  6. Khatri K, Pu Y, Klein JA, Wei J, Costello CE, Lin C, Zaia J. Comparison of Collisional and Electron-Based Dissociation Modes for Middle-Down Analysis of Multiply Glycosylated Peptides. J Am Soc Mass Spectrom. 2018 Jun; 29(6):1075-1085.View Related Profiles. PMID: 29663256.
     
  7. Hogan JD, Klein JA, Wu J, Chopra P, Boons GJ, Carvalho L, Lin C, Zaia J. Software for Peak Finding and Elemental Composition Assignment for Glycosaminoglycan Tandem Mass Spectra. Mol Cell Proteomics. 2018 Jul; 17(7):1448-1456. PMID: 29615495.
     
  8. Wu J, Wei J, Hogan JD, Chopra P, Joshi A, Lu W, Klein J, Boons GJ, Lin C, Zaia J. Negative Electron Transfer Dissociation Sequencing of 3-O-Sulfation-Containing Heparan Sulfate Oligosaccharides. J Am Soc Mass Spectrom. 2018 Jun; 29(6):1262-1272. PMID: 29564812.
     
  9. Turiák L, Tóth G, Ozohanics O, Révész Á, Ács A, Vékey K, Zaia J, Drahos L. Sensitive method for glycosaminoglycan analysis of tissue sections. J Chromatogr A. 2018 Apr 06; 1544:41-48.View Related Profiles. PMID: 29506752.
     
  10. Wu ZL, Person AD, Anderson M, Burroughs B, Tatge T, Khatri K, Zou Y, Wang L, Geders T, Zaia J, Sackstein R. Imaging specific cellular glycan structures using glycosyltransferases via click chemistry. Glycobiology. 2018 02 01; 28(2):69-79.View Related Profiles. PMID: 29186441.
     
Showing 10 of 167 results. Show More

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 167 publications over 29 distinct years, with a maximum of 22 publications in 2011

YearPublications
19851
19872
19911
19921
19943
19951
19964
19971
19983
19993
20003
20014
20022
20034
20043
20053
20068
20072
20087
20098
201011
201122
201211
201316
201410
20158
201610
20175
201810
In addition to these self-described keywords below, a list of MeSH based concepts is available here.

glycomics
glycosaminoglycan
heparan sulfate
heparin
mass spectrometry
proteoglycan
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670 Albany St Biosquare III
Boston MA 02118
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