Keywords
Last Name

Joseph Zaia, PhD

TitleProfessor
InstitutionBoston University School of Medicine
DepartmentBiochemistry
Address670 Albany St Biosquare III
Boston MA 02118
Phone(617) 638-6762
ORCID ORCID Icon0000-0001-9497-8701
Other Positions
TitleGraduate Faculty (Primary Mentor of Grad Students)
InstitutionBoston University School of Medicine, Division of Graduate Medical Sciences

TitleMember
InstitutionBoston University
DepartmentBioinformatics Graduate Program

TitleCenter Faculty Member
InstitutionBoston University School of Medicine
DepartmentMass Spectrometry

 Research Expertise & Professional Interests
The manner in which a cell responds to many growth factor stimuli depends on interactions between glycosaminoglycans (GAGs), growth factors, and growth factor receptors. Extracellular matrix GAGs binds growth factors, creating morphogens gradients essential to tissue patterning. Because these events depend on the fine structure of the GAG chains present, regulation of GAG biosynthesis is a key factor for understanding normal and disease related cellular growth
The key to exploiting an understanding of GAG structure-function relationships for human disease therapy is to winnow oligosaccharide-protein binding patterns from heterogeneous biological preparations. Toward this end, we have developed mass spectral methods for GAGs that enable comparison of structures as a function of biological variables.

The long term research aims are (1) to develop a fundamental understanding of the manner in which glycosaminoglycan expression is varied according to the cellular growth environment related to human disease and (2) to identify HS chain structures useful as therapeutic targets.

New bioinformatics methods are essential to realizing these goals. The data produced using our methods are information rich and not amenable to manual interpretation. Further, the methods needed are distinct from those used in genomics and proteomics. We are developing bioinformatics methods appropriate for interpretation of structural data on glycosaminoglycans and other carbohydrates to identify targets for disease therapy.

 Self-Described Keywords
  • glycomics
  • glycosaminoglycan
  • heparan sulfate
  • heparin
  • mass spectrometry
  • proteoglycan
 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Glaskin RS, Khatri K, Wang Q, Zaia J, Costello CE. Construction of a Database of Collision Cross Section Values for Glycopeptides, Glycans, and Peptides Determined by IM-MS. Anal Chem. 2017 Mar 21. PMID: 28323417.
    View in: PubMed
  2. Wu ZL, Huang X, Ethen CM, Tatge T, Pasek M, Zaia J. Non-reducing end labeling of heparan sulfate via click chemistry and a high throughput ELISA assay for heparanase. Glycobiology. 2017 Feb 06; 1-7. PMID: 28173134.
    View in: PubMed
  3. Wu ZL, Huang X, Ethen C, Tatge T, Pasek M, Zaia J. Non-reducing end labeling of heparan sulfate via click chemistry and a high throughput ELISA assay for heparanase. Glycobiology. 2016 Dec 26. PMID: 28025251.
    View in: PubMed
  4. Liu Y, McBride R, Stoll M, Palma AS, Silva L, Agravat S, Aoki-Kinoshita KF, Campbell MP, Costello CE, Dell A, Haslam SM, Karlsson NG, Khoo KH, Kolarich D, Novotny MV, Packer NH, Ranzinger R, Rapp E, Rudd PM, Struwe WB, Tiemeyer M, Wells L, York WS, Zaia J, Kettner C, Paulson JC, Feizi T, Smith DF. The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data. Glycobiology. 2016 Nov 22. PMID: 27993942.
    View in: PubMed
  5. Zaia J, Khatri K, Klein J, Shao C, Sheng Y, Viner R. Complete Molecular Weight Profiling of Low-Molecular Weight Heparins Using Size Exclusion Chromatography-Ion Suppressor-High-Resolution Mass Spectrometry. Anal Chem. 2016 Nov 01; 88(21):10654-10660. PMID: 27709905.
    View in: PubMed
  6. Khatri K, Klein JA, Zaia J. Use of an informed search space maximizes confidence of site-specific assignment of glycoprotein glycosylation. Anal Bioanal Chem. 2017 Jan; 409(2):607-618. PMID: 27734143.
    View in: PubMed
  7. Dierker T, Shao C, Haitina T, Zaia J, Hinas A, Kjellén L. Nematodes join the family of chondroitin sulfate-synthesizing organisms: Identification of an active chondroitin sulfotransferase in Caenorhabditis elegans. Sci Rep. 2016 Oct 05; 6:34662. PMID: 27703236.
    View in: PubMed
  8. Struwe WB, Agravat S, Aoki-Kinoshita KF, Campbell MP, Costello CE, Dell A. The minimum information required for a glycomics experiment (MIRAGE) project: sample preparation guidelines for reliable reporting of glycomics datasets. Glycobiology. 2016 Sep; 26(9):907-910. PMID: 27654115.
    View in: PubMed
  9. Sethi MK, Zaia J. Extracellular matrix proteomics in schizophrenia and Alzheimer's disease. Anal Bioanal Chem. 2017 Jan; 409(2):379-394. PMID: 27601046.
    View in: PubMed
  10. He H, Liu D, Lin H, Jiang S, Ying Y, Chun S, Deng H, Zaia J, Wen R, Luo Z. Phosphatidylethanolamine binding protein 4 (PEBP4) is a secreted protein and has multiple functions. Biochim Biophys Acta. 2016 Jul; 1863(7 Pt A):1682-9. PMID: 27033522.
    View in: PubMed
  11. Khatri K, Klein JA, White MR, Grant OC, Leymarie N, Woods RJ, Hartshorn KL, Zaia J. Integrated Omics and Computational Glycobiology Reveal Structural Basis for Influenza A Virus Glycan Microheterogeneity and Host Interactions. Mol Cell Proteomics. 2016 Jun; 15(6):1895-912. PMID: 26984886.
    View in: PubMed
  12. Hu H, Khatri K, Zaia J. Algorithms and design strategies towards automated glycoproteomics analysis. Mass Spectrom Rev. 2016 Jan 04. PMID: 26728195.
    View in: PubMed
  13. Tykesson E, Mao Y, Maccarana M, Pu Y, Gao J, Lin C, Zaia J, Westergren-Thorsson G, Ellervik U, Malmström L, Malmström A. Deciphering the Mode of Action of the Processive Polysaccharide Modifying Enzyme Dermatan Sulfate Epimerase 1 by Hydrogen-Deuterium Exchange Mass Spectrometry. Chem Sci. 2016 Feb 01; 7(2):1447-1456. PMID: 26900446.
    View in: PubMed
  14. Hu H, Khatri K, Klein J, Leymarie N, Zaia J. A review of methods for interpretation of glycopeptide tandem mass spectral data. Glycoconj J. 2016 Jun; 33(3):285-96. PMID: 26612686.
    View in: PubMed
  15. Aoki-Kinoshita K, Agravat S, Aoki NP, Arpinar S, Cummings RD, Fujita A, Fujita N, Hart GM, Haslam SM, Kawasaki T, Matsubara M, Moreman KW, Okuda S, Pierce M, Ranzinger R, Shikanai T, Shinmachi D, Solovieva E, Suzuki Y, Tsuchiya S, Yamada I, York WS, Zaia J, Narimatsu H. GlyTouCan 1.0--The international glycan structure repository. Nucleic Acids Res. 2016 Jan 4; 44(D1):D1237-42. PMID: 26476458.
    View in: PubMed
  16. Salanti A, Clausen TM, Agerbæk MØ, Al Nakouzi N, Dahlbäck M, Oo HZ, Lee S, Gustavsson T, Rich JR, Hedberg BJ, Mao Y, Barington L, Pereira MA, LoBello J, Endo M, Fazli L, Soden J, Wang CK, Sander AF, Dagil R, Thrane S, Holst PJ, Meng L, Favero F, Weiss GJ, Nielsen MA, Freeth J, Nielsen TO, Zaia J, Tran NL, Trent J, Babcook JS, Theander TG, Sorensen PH, Daugaard M. Targeting Human Cancer by a Glycosaminoglycan Binding Malaria Protein. Cancer Cell. 2015 Oct 12; 28(4):500-14. PMID: 26461094.
    View in: PubMed
  17. Huang Y, Mao Y, Zong C, Lin C, Boons GJ, Zaia J. Discovery of a heparan sulfate 3-O-sulfation specific peeling reaction. Anal Chem. 2015 Jan 6; 87(1):592-600. PMID: 25486437.
    View in: PubMed
  18. Reine TM, Kolseth IB, Meen AJ, Lindahl JP, Jenssen TG, Reinholt FP, Zaia J, Shao C, Hartmann A, Kolset SO. Effects of restoring normoglycemia in type 1 diabetes on inflammatory profile and renal extracellular matrix structure after simultaneous pancreas and kidney transplantation. Diabetes Res Clin Pract. 2015 Jan; 107(1):46-53. PMID: 25467621.
    View in: PubMed
  19. Mao Y, Huang Y, Buczek-Thomas JA, Ethen CM, Nugent MA, Wu ZL, Zaia J. A liquid chromatography-mass spectrometry-based approach to characterize the substrate specificity of mammalian heparanase. J Biol Chem. 2014 Dec 5; 289(49):34141-51. PMID: 25336655.
    View in: PubMed
  20. Turiák L, Shao C, Meng L, Khatri K, Leymarie N, Wang Q, Pantazopoulos H, Leon DR, Zaia J. Workflow for combined proteomics and glycomics profiling from histological tissues. Anal Chem. 2014 Oct 7; 86(19):9670-8. PMID: 25203838.
    View in: PubMed
  21. Khatri K, Staples GO, Leymarie N, Leon DR, Turiák L, Huang Y, Yip S, Hu H, Heckendorf CF, Zaia J. Confident assignment of site-specific glycosylation in complex glycoproteins in a single step. J Proteome Res. 2014 Oct 3; 13(10):4347-55. PMID: 25153361.
    View in: PubMed
  22. Huang Y, Mao Y, Buczek-Thomas JA, Nugent MA, Zaia J. Oligosaccharide substrate preferences of human extracellular sulfatase Sulf2 using liquid chromatography-mass spectrometry based glycomics approaches. PLoS One. 2014; 9(8):e105143. PMID: 25127119.
    View in: PubMed
  23. Hu H, Huang Y, Mao Y, Yu X, Xu Y, Liu J, Zong C, Boons GJ, Lin C, Xia Y, Zaia J. A computational framework for heparan sulfate sequencing using high-resolution tandem mass spectra. Mol Cell Proteomics. 2014 Sep; 13(9):2490-502. PMID: 24925905.
    View in: PubMed
  24. Wu ZL, Robey MT, Tatge T, Lin C, Leymarie N, Zou Y, Zaia J. Detecting O-GlcNAc using in vitro sulfation. Glycobiology. 2014 Aug; 24(8):740-7. PMID: 24799377.
    View in: PubMed
  25. York WS, Agravat S, Aoki-Kinoshita KF, McBride R, Campbell MP, Costello CE, Dell A, Feizi T, Haslam SM, Karlsson N, Khoo KH, Kolarich D, Liu Y, Novotny M, Packer NH, Paulson JC, Rapp E, Ranzinger R, Rudd PM, Smith DF, Struwe WB, Tiemeyer M, Wells L, Zaia J, Kettner C. MIRAGE: the minimum information required for a glycomics experiment. Glycobiology. 2014 May; 24(5):402-6. PMID: 24653214.
    View in: PubMed
  26. Hu H, Dittwald P, Zaia J, Valkenborg D. Comment on "Computation of isotopic peak center-mass distribution by fourier transform". Anal Chem. 2013 Dec 17; 85(24):12189-92. PMID: 24187947.
    View in: PubMed
  27. Huang Y, Yu X, Mao Y, Costello CE, Zaia J, Lin C. De novo sequencing of heparan sulfate oligosaccharides by electron-activated dissociation. Anal Chem. 2013 Dec 17; 85(24):11979-86. PMID: 24224699.
    View in: PubMed
  28. Mathias DK, Pastrana-Mena R, Ranucci E, Tao D, Ferruti P, Ortega C, Staples GO, Zaia J, Takashima E, Tsuboi T, Borg NA, Verotta L, Dinglasan RR. A small molecule glycosaminoglycan mimetic blocks Plasmodium invasion of the mosquito midgut. PLoS Pathog. 2013; 9(11):e1003757. PMID: 24278017.
    View in: PubMed
  29. Ramachandra R, Namburi RB, Ortega-Martinez O, Shi X, Zaia J, Dupont ST, Thorndyke MC, Lindahl U, Spillmann D. Brittlestars contain highly sulfated chondroitin sulfates/dermatan sulfates that promote fibroblast growth factor 2-induced cell signaling. Glycobiology. 2014 Feb; 24(2):195-207. PMID: 24253764.
    View in: PubMed
  30. Lu Z, Rynkiewicz MJ, Yang CY, Madico G, Perkins HM, Wang Q, Costello CE, Zaia J, Seaton BA, Sharon J. The binding sites of monoclonal antibodies to the non-reducing end of Francisella tularensis O-antigen accommodate mainly the terminal saccharide. Immunology. 2013 Nov; 140(3):374-89. PMID: 23844703.
    View in: PubMed
  31. Shao C, Shi X, Phillips JJ, Zaia J. Mass spectral profiling of glycosaminoglycans from histological tissue surfaces. Anal Chem. 2013 Nov 19; 85(22):10984-91. PMID: 24099043.
    View in: PubMed
  32. Nugent MA, Zaia J, Spencer JL. Heparan sulfate-protein binding specificity. Biochemistry (Mosc). 2013 Jul; 78(7):726-35. PMID: 24010836.
    View in: PubMed
  33. Leymarie N, Griffin PJ, Jonscher K, Kolarich D, Orlando R, McComb M, Zaia J, Aguilan J, Alley WR, Altmann F, Ball LE, Basumallick L, Bazemore-Walker CR, Behnken H, Blank MA, Brown KJ, Bunz SC, Cairo CW, Cipollo JF, Daneshfar R, Desaire H, Drake RR, Go EP, Goldman R, Gruber C, Halim A, Hathout Y, Hensbergen PJ, Horn DM, Hurum D, Jabs W, Larson G, Ly M, Mann BF, Marx K, Mechref Y, Meyer B, Möginger U, Neusüß C, Nilsson J, Novotny MV, Nyalwidhe JO, Packer NH, Pompach P, Reiz B, Resemann A, Rohrer JS, Ruthenbeck A, Sanda M, Schulz JM, Schweiger-Hufnagel U, Sihlbom C, Song E, Staples GO, Suckau D, Tang H, Thaysen-Andersen M, Viner RI, An Y, Valmu L, Wada Y, Watson M, Windwarder M, Whittal R, Wuhrer M, Zhu Y, Zou C. Interlaboratory study on differential analysis of protein glycosylation by mass spectrometry: the ABRF glycoprotein research multi-institutional study 2012. Mol Cell Proteomics. 2013 Oct; 12(10):2935-51. PMID: 23764502.
    View in: PubMed
  34. Shi X, Shao C, Mao Y, Huang Y, Wu ZL, Zaia J. LC-MS and LC-MS/MS studies of incorporation of 34SO3 into glycosaminoglycan chains by sulfotransferases. Glycobiology. 2013 Aug; 23(8):969-79. PMID: 23696150.
    View in: PubMed
  35. Shao C, Shi X, White M, Huang Y, Hartshorn K, Zaia J. Comparative glycomics of leukocyte glycosaminoglycans. FEBS J. 2013 May; 280(10):2447-61. PMID: 23480678.
    View in: PubMed
  36. Kolarich D, Rapp E, Struwe WB, Haslam SM, Zaia J, McBride R, Agravat S, Campbell MP, Kato M, Ranzinger R, Kettner C, York WS. The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting mass-spectrometry-based glycoanalytic data. Mol Cell Proteomics. 2013 Apr; 12(4):991-5. PMID: 23378518.
    View in: PubMed
  37. Zaia J. Glycosaminoglycan glycomics using mass spectrometry. Mol Cell Proteomics. 2013 Apr; 12(4):885-92. PMID: 23325770.
    View in: PubMed
  38. Gill VL, Aich U, Rao S, Pohl C, Zaia J. Disaccharide analysis of glycosaminoglycans using hydrophilic interaction chromatography and mass spectrometry. Anal Chem. 2013 Jan 15; 85(2):1138-45. PMID: 23234263.
    View in: PubMed
  39. Zaia J. Capillary electrophoresis-mass spectrometry of carbohydrates. Methods Mol Biol. 2013; 984:13-25. PMID: 23386333.
    View in: PubMed
  40. Maxwell E, Tan Y, Tan Y, Hu H, Benson G, Aizikov K, Conley S, Staples GO, Slysz GW, Smith RD, Zaia J. GlycReSoft: a software package for automated recognition of glycans from LC/MS data. PLoS One. 2012; 7(9):e45474. PMID: 23049804.
    View in: PubMed
  41. Li L, Zhang F, Zaia J, Linhardt RJ. Top-down approach for the direct characterization of low molecular weight heparins using LC-FT-MS. Anal Chem. 2012 Oct 16; 84(20):8822-9. PMID: 22985071.
    View in: PubMed
  42. Gill VL, Wang Q, Shi X, Zaia J. Mass spectrometric method for determining the uronic acid epimerization in heparan sulfate disaccharides generated using nitrous acid. Anal Chem. 2012 Sep 4; 84(17):7539-46. PMID: 22873817.
    View in: PubMed
  43. Tran TH, Shi X, Zaia J, Ai X. Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration. J Biol Chem. 2012 Sep 21; 287(39):32651-64. PMID: 22865881.
    View in: PubMed
  44. Shi X, Huang Y, Mao Y, Naimy H, Zaia J. Tandem mass spectrometry of heparan sulfate negative ions: sulfate loss patterns and chemical modification methods for improvement of product ion profiles. J Am Soc Mass Spectrom. 2012 Sep; 23(9):1498-511. PMID: 22825743.
    View in: PubMed
  45. Lu Z, Madico G, Roche MI, Wang Q, Hui JH, Perkins HM, Zaia J, Costello CE, Sharon J. Protective B-cell epitopes of Francisella tularensis O-polysaccharide in a mouse model of respiratory tularaemia. Immunology. 2012 Jul; 136(3):352-60. PMID: 22486311.
    View in: PubMed
  46. Leymarie N, Zaia J. Effective use of mass spectrometry for glycan and glycopeptide structural analysis. Anal Chem. 2012 Apr 3; 84(7):3040-8. PMID: 22360375.
    View in: PubMed
  47. Thelin MA, Svensson KJ, Shi X, Bagher M, Axelsson J, Isinger-Ekstrand A, van Kuppevelt TH, Johansson J, Nilbert M, Zaia J, Belting M, Maccarana M, Malmström A. Dermatan sulfate is involved in the tumorigenic properties of esophagus squamous cell carcinoma. Cancer Res. 2012 Apr 15; 72(8):1943-52. PMID: 22350411.
    View in: PubMed
  48. Staples GO, Zaia J. Analysis of Glycosaminoglycans Using Mass Spectrometry. Curr Proteomics. 2011; 8:325-336.
    View in: External Website
  49. Wang Q, Shi X, Leymarie N, Madico G, Sharon J, Costello CE, Zaia J. A typical preparation of Francisella tularensis O-antigen yields a mixture of three types of saccharides. Biochemistry. 2011 Dec 20; 50(50):10941-50. PMID: 22091710.
    View in: PubMed
  50. Huang Y, Shi X, Yu X, Leymarie N, Staples GO, Yin H, Killeen K, Zaia J. Improved liquid chromatography-MS/MS of heparan sulfate oligosaccharides via chip-based pulsed makeup flow. Anal Chem. 2011 Nov 1; 83(21):8222-9. PMID: 21923145.
    View in: PubMed
  51. Crouch E, Nikolaidis N, McCormack FX, McDonald B, Allen K, Rynkiewicz MJ, Cafarella TM, White M, Lewnard K, Leymarie N, Zaia J, Seaton BA, Hartshorn KL. Mutagenesis of surfactant protein D informed by evolution and x-ray crystallography enhances defenses against influenza A virus in vivo. J Biol Chem. 2011 Nov 25; 286(47):40681-92. PMID: 21965658.
    View in: PubMed
  52. Bielik AM, Zaia J. Multistage Tandem Mass Spectrometry of Chondroitin Sulfate and Dermatan Sulfate. Int J Mass Spectrom. 2011 Aug 15; 305(2-3):131-137. PMID: 21860601.
    View in: PubMed
  53. Leymarie N, McComb ME, Naimy H, Staples GO, Zaia J. Differential Characterization and Classification of Tissue Specific Glycosaminoglycans by Tandem Mass Spectrometry and Statistical Methods. Int J Mass Spectrom. 2012 Feb 15; 312:144-154. PMID: 22523474.
    View in: PubMed
  54. Schumacher VA, Schlötzer-Schrehardt U, Karumanchi SA, Shi X, Zaia J, Jeruschke S, Zhang D, Pavenstädt H, Pavenstaedt H, Drenckhan A, Amann K, Ng C, Hartwig S, Ng KH, Ho J, Kreidberg JA, Taglienti M, Royer-Pokora B, Ai X. WT1-dependent sulfatase expression maintains the normal glomerular filtration barrier. J Am Soc Nephrol. 2011 Jul; 22(7):1286-96. PMID: 21719793.
    View in: PubMed
  55. Naimy H, Buczek-Thomas JA, Nugent MA, Leymarie N, Zaia J. Highly sulfated nonreducing end-derived heparan sulfate domains bind fibroblast growth factor-2 with high affinity and are enriched in biologically active fractions. J Biol Chem. 2011 Jun 3; 286(22):19311-9. PMID: 21471211.
    View in: PubMed
  56. Staples GO, Shi X, Zaia J. Glycomics analysis of mammalian heparan sulfates modified by the human extracellular sulfatase HSulf2. PLoS One. 2011; 6(2):e16689. PMID: 21347431.
    View in: PubMed
  57. Staples GO, Zaia J. In Carbohydrate Recognition; Wang B, Boons GJ, Eds. John Wiley & Sons, Inc. Hoboken. 2011; 257-300.
  58. Zaia J. At last, functional glycomics. Nat Methods. 2011 Jan; 8(1):55-7. PMID: 21191374.
    View in: PubMed
  59. Staples GO, Zaia J. Analysis of Glycosaminoglycans Using Mass Spectrometry. Curr Proteomics. 2011; 8(4):325-336. PMID: 25705143.
    View in: PubMed
  60. Dreyfuss JM, Jacobs C, Gindin Y, Benson G, Staples GO, Zaia J. Targeted analysis of glycomics liquid chromatography/mass spectrometry data. Anal Bioanal Chem. 2011 Jan; 399(2):727-35. PMID: 20953780.
    View in: PubMed
  61. Langsdorf A, Schumacher V, Shi X, Tran T, Zaia J, Jain S, Taglienti M, Kreidberg JA, Fine A, Ai X. Expression regulation and function of heparan sulfate 6-O-endosulfatases in the spermatogonial stem cell niche. Glycobiology. 2011 Feb; 21(2):152-61. PMID: 20855470.
    View in: PubMed
  62. Zaia J. Mass spectrometry and glycomics. OMICS. 2010 Aug; 14(4):401-18. PMID: 20443730.
    View in: PubMed
  63. Naimy H, Leymarie N, Zaia J. Screening for anticoagulant heparan sulfate octasaccharides and fine structure characterization using tandem mass spectrometry. Biochemistry. 2010 May 4; 49(17):3743-52. PMID: 20345121.
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  64. Staples GO, Shi X, Zaia J. Extended N-sulfated domains reside at the nonreducing end of heparan sulfate chains. J Biol Chem. 2010 Jun 11; 285(24):18336-43. PMID: 20363743.
    View in: PubMed
  65. Bowman MJ, Zaia J. Comparative glycomics using a tetraplex stable-isotope coded tag. Anal Chem. 2010 Apr 1; 82(7):3023-31. PMID: 20230064.
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  66. Staples GO, Naimy H, Yin H, Kileen K, Kraiczek K, Costello CE, Zaia J. Improved hydrophilic interaction chromatography LC/MS of heparinoids using a chip with postcolumn makeup flow. Anal Chem. 2010 Jan 15; 82(2):516-22. PMID: 20000724.
    View in: PubMed
  67. Bielik AM, Zaia J. Historical overview of glycoanalysis. Methods Mol Biol. 2010; 600:9-30. PMID: 19882118.
    View in: PubMed
  68. Bielik AM, Zaia J. Extraction of chondroitin/dermatan sulfate glycosaminoglycans from connective tissue for mass spectrometric analysis. Methods Mol Biol. 2010; 600:215-25. PMID: 19882131.
    View in: PubMed
  69. Zaia J. On-line separations combined with MS for analysis of glycosaminoglycans. Mass Spectrom Rev. 2009 Mar-Apr; 28(2):254-72. PMID: 18956477.
    View in: PubMed
  70. Shi X, Zaia J. Organ-specific heparan sulfate structural phenotypes. J Biol Chem. 2009 May 1; 284(18):11806-14. PMID: 19244235.
    View in: PubMed
  71. Staples GO, Bowman MJ, Costello CE, Hitchcock AM, Lau JM, Leymarie N, Miller C, Naimy H, Shi X, Zaia J. A chip-based amide-HILIC LC/MS platform for glycosaminoglycan glycomics profiling. Proteomics. 2009 Feb; 9(3):686-95. PMID: 19137549.
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  72. Hitchcock AM, Bowman MJ, Staples GO, Zaia J. Improved workup for glycosaminoglycan disaccharide analysis using CE with LIF detection. Electrophoresis. 2008 Nov; 29(22):4538-48. PMID: 19035406.
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  73. Zaia J. Mass spectrometry and the emerging field of glycomics. Chem Biol. 2008 Sep 22; 15(9):881-92. PMID: 18804025.
    View in: PubMed
  74. Hitchcock AM, Yates KE, Costello CE, Zaia J. Comparative glycomics of connective tissue glycosaminoglycans. Proteomics. 2008 Apr; 8(7):1384-97. PMID: 18318007.
    View in: PubMed
  75. Naimy H, Leymarie N, Bowman MJ, Zaia J. Characterization of heparin oligosaccharides binding specifically to antithrombin III using mass spectrometry. Biochemistry. 2008 Mar 11; 47(10):3155-61. PMID: 18260648.
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  76. Bowman MJ, Zaia J. Tags for the stable isotopic labeling of carbohydrates and quantitative analysis by mass spectrometry. Anal Chem. 2007 Aug 1; 79(15):5777-84. PMID: 17605469.
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  77. Zaia J, Miller MJ, Seymour JL, Costello CE. The role of mobile protons in negative ion CID of oligosaccharides. J Am Soc Mass Spectrom. 2007 May; 18(5):952-60. PMID: 17383193.
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  78. Hitchcock AM, Yates KE, Shortkroff S, Costello CE, Zaia J. Optimized extraction of glycosaminoglycans from normal and osteoarthritic cartilage for glycomics profiling. Glycobiology. 2007 Jan; 17(1):25-35. PMID: 16980326.
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  79. Ziegler A, Zaia J. Size-exclusion chromatography of heparin oligosaccharides at high and low pressure. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jun 6; 837(1-2):76-86. PMID: 16704936.
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  80. Seymour JL, Costello CE, Zaia J. The influence of sialylation on glycan negative ion dissociation and energetics. J Am Soc Mass Spectrom. 2006 Jun; 17(6):844-54. PMID: 16603372.
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  81. Hitchcock AM, Costello CE, Zaia J. Glycoform quantification of chondroitin/dermatan sulfate using a liquid chromatography-tandem mass spectrometry platform. Biochemistry. 2006 Feb 21; 45(7):2350-61. PMID: 16475824.
    View in: PubMed
  82. Miller MJ, Costello CE, Malmström A, Zaia J. A tandem mass spectrometric approach to determination of chondroitin/dermatan sulfate oligosaccharide glycoforms. Glycobiology. 2006 Jun; 16(6):502-13. PMID: 16489125.
    View in: PubMed
  83. Zaia J. In Ionization Methods; Gross ML, Caprioli RM, Eds. Elsevier. Amsterdam. 2006; 6:889-903.
  84. Zaia J. Principles of Mass Spectrometry of Glycosaminoglycans. Journal of Biomacromolecular Mass Spectrometry. 2005; 1:3-36.
  85. Naggar EF, Costello CE, Zaia J. Competing fragmentation processes in tandem mass spectra of heparin-like glycosaminoglycans. J Am Soc Mass Spectrom. 2004 Nov; 15(11):1534-44. PMID: 15519220.
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  86. Zaia J. Mass spectrometry of oligosaccharides. Mass Spectrom Rev. 2004 May-Jun; 23(3):161-227. PMID: 14966796.
    View in: PubMed
  87. Zaia J, Li XQ, Chan SY, Costello CE. Tandem mass spectrometric strategies for determination of sulfation positions and uronic acid epimerization in chondroitin sulfate oligosaccharides. J Am Soc Mass Spectrom. 2003 Nov; 14(11):1270-81. PMID: 14597117.
    View in: PubMed
  88. Zaia J, Costello CE. Tandem mass spectrometry of sulfated heparin-like glycosaminoglycan oligosaccharides. Anal Chem. 2003 May 15; 75(10):2445-55. PMID: 12918989.
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  89. McClellan JE, Costello CE, O'Connor PB, Zaia J. Influence of charge state on product ion mass spectra and the determination of 4S/6S sulfation sequence of chondroitin sulfate oligosaccharides. Anal Chem. 2002 Aug 1; 74(15):3760-71. PMID: 12175164.
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  90. Zaia J, McClellan JE, Costello CE. Tandem mass spectrometric determination of the 4S/6S sulfation sequence in chondroitin sulfate oligosaccharides. Anal Chem. 2001 Dec 15; 73(24):6030-9. PMID: 11791576.
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  91. Barry F, Boynton R, Murphy M, Haynesworth S, Zaia J. The SH-3 and SH-4 antibodies recognize distinct epitopes on CD73 from human mesenchymal stem cells. Biochem Biophys Res Commun. 2001 Nov 30; 289(2):519-24. PMID: 11716504.
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  92. Zaia J, Boynton R, Heinegård D, Barry F. Posttranslational modifications to human bone sialoprotein determined by mass spectrometry. Biochemistry. 2001 Oct 30; 40(43):12983-91. PMID: 11669636.
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  93. Zaia J, Costello CE. Compositional analysis of glycosaminoglycans by electrospray mass spectrometry. Anal Chem. 2001 Jan 15; 73(2):233-9. PMID: 11199971.
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  94. Cheng X, Cole RN, Zaia J, Hart GW. Alternative O-glycosylation/O-phosphorylation of the murine estrogen receptor beta. Biochemistry. 2000 Sep 26; 39(38):11609-20. PMID: 10995228.
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  95. Zaia J, Liu B, Boynton R, Barry F. Structural analysis of cartilage proteoglycans and glycoproteins using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Biochem. 2000 Jan 1; 277(1):94-103. PMID: 10610693.
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  96. Barry FP, Boynton RE, Haynesworth S, Murphy JM, Zaia J. The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105). Biochem Biophys Res Commun. 1999 Nov; 265(1):134-9. PMID: 10548503.
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  97. Fenselau C, Antoine MD, Fabris D, Hathout Y, He T, Zaia J. In Metallothionein IV; Klaasen C, Ed. Birkhauser. Basel. 1999; 577-580.
  98. Zaia J, Fabris D, Wei D, Karpel RL, Fenselau C. Monitoring metal ion flux in reactions of metallothionein and drug-modified metallothionein by electrospray mass spectrometry. Protein Sci. 1998 Nov; 7(11):2398-404. PMID: 9828006.
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  99. van den Bos C, Rammes A, Vogl T, Boynton R, Zaia J, Sorg C, Roth J. Copurification of P6, MRP8, and MRP14 from human granulocytes and separation of individual proteins. Protein Expr Purif. 1998 Aug; 13(3):313-8. PMID: 9693055.
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  100. Bruder SP, Ricalton NS, Boynton RE, Connolly TJ, Jaiswal N, Zaia J, Barry FP. Mesenchymal stem cell surface antigen SB-10 corresponds to activated leukocyte cell adhesion molecule and is involved in osteogenic differentiation. J Bone Miner Res. 1998 Apr; 13(4):655-63. PMID: 9556065.
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  101. Zaia J, Boynton RE, McIntosh A, Marshak DR, Olsson H, Heinegârd D, Barry FP. Post-translational modifications in cartilage oligomeric matrix protein. Characterization of the N-linked oligosaccharides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. J Biol Chem. 1997 May 30; 272(22):14120-6. PMID: 9162039.
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  102. Zaia J, Jiang L, Han MS, Tabb JR, Wu Z, Fabris D, Fenselau C. A binding site for chlorambucil on metallothionein. Biochemistry. 1996 Mar 5; 35(9):2830-5. PMID: 8608118.
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  103. Fabris D, Zaia J, Hathout Y, Fenselau C. Retention of thiol protons in two classes of protein zinc ion coordination centers. J Am Chem Soc. 1996; 118:12242-12243.
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  104. Zaia J. Charged derivatives for peptide sequencing using a magnetic sector instrument. Methods Mol Biol. 1996; 61:29-41. PMID: 8930863.
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  105. Zaia J, Biemann K. Comparison of charged derivatives for high energy collision-induced dissociation tandem mass spectrometry. J Am Soc Mass Spectrom. 1995; 6(5):428-436.
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  106. Day BW, Skipper PL, Zaia J, Singh K, Tannenbaum SR. Enantiospecificity of covalent adduct formation by benzo[a]pyrene anti-diol epoxide with human serum albumin. Chem Res Toxicol. 1994 Nov-Dec; 7(6):829-35. PMID: 7696539.
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  107. Zaia J, Biemann K. Characteristics of high energy collision-induced dissociation tandem mass spectra of polycyclic aromatic hydrocarbon diolepoxide adducted peptides. J Am Soc Mass Spectrom. 1994; 5(7):649-654.
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  108. Zaia J, Biemann K. Proteinase K activity inhibited near amino acids carrying large substituents: three PAH diolepoxides covalently modify His-146 human serum albumin residues. J Am Chem Soc. 1994; 116:7407-7408.
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  109. Zaia J, Annan RS, Biemann K. The correct molecular weight of myoglobin, a common calibrant for mass spectrometry. Rapid Commun Mass Spectrom. 1992 Jan; 6(1):32-6. PMID: 1591398.
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  110. Day BW, Skipper PL, Zaia J, Tannenbaum SR. Benzo[a]pyrene anti-diol epoxide covalently modifies human serum albumin carboxylate side chains and imidazole side chains of histidine(146). J Am Chem Soc. 1991; 113:8505-8509.
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  111. Nicholson-Weller A, Zaia J, Raum MG, Coligan JE. Decay accelerating factor (DAF) peptide sequences share homology with a consensus sequence found in the superfamily of structurally related complement proteins and other proteins including haptoglobin, factor XIII, beta 2-glycoprotein I, and the IL-2 receptor. Immunol Lett. 1987 Apr; 14(4):307-11. PMID: 2438213.
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  112. Wenzel TJ, Zaia J. Organic-Soluble Lanthanide Nuclear-Magnetic-Resonance Shift-Reagents for Sulfonium and Isothiouronium Salts. Anal Chem. 1987; 59:562-567.
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  113. Wenzel TJ, Zaia J. Lanthanide Tetrakis (Beta-Diketonates) as Effective NMR Shift-Reagents for Organic Salts. J Org Chem. 1985; 50:1322-1324.
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