Joseph Zaia, PhD
Professor
Boston University School of Medicine
Dept of Biochemistry

PhD, Massachusetts Institute of Technology



The manner in which a cell responds to many growth factor stimuli depends on interactions between glycosaminoglycans (GAGs), growth factors, and growth factor receptors. Extracellular matrix GAGs binds growth factors, creating morphogens gradients essential to tissue patterning. Because these events depend on the fine structure of the GAG chains present, regulation of GAG biosynthesis is a key factor for understanding normal and disease related cellular growth
The key to exploiting an understanding of GAG structure-function relationships for human disease therapy is to winnow oligosaccharide-protein binding patterns from heterogeneous biological preparations. Toward this end, we have developed mass spectral methods for GAGs that enable comparison of structures as a function of biological variables.

The long term research aims are (1) to develop a fundamental understanding of the manner in which glycosaminoglycan expression is varied according to the cellular growth environment related to human disease and (2) to identify HS chain structures useful as therapeutic targets.

New bioinformatics methods are essential to realizing these goals. The data produced using our methods are information rich and not amenable to manual interpretation. Further, the methods needed are distinct from those used in genomics and proteomics. We are developing bioinformatics methods appropriate for interpretation of structural data on glycosaminoglycans and other carbohydrates to identify targets for disease therapy.

Graduate Faculty (Primary Mentor of Grad Students)
Boston University School of Medicine, Division of Graduate Medical Sciences


Member
Boston University
Department of Bioinformatics Graduate Program


Center Faculty Member
Boston University School of Medicine
Department of Mass Spectrometry




Software for Automated Interpretation of Heparan Sulfate Tandem Mass Spectra
09/15/2015 - 06/30/2018 (PI)
NIH/National Heart, Lung, and Blood Institute
3R21HL131554-02S1

Quantitative Profiling of Glycosaminoglycans from Breast Tumor Tissue Arrays
07/09/2014 - 06/30/2018 (PI)
NIH/National Cancer Institute
5R21CA177479-03

Viral Vector Transduction Deficiencies in the Aged Nigrostriatal System
09/30/2016 - 06/30/2017 (PI)
Michigan State University NIH NIA
1R56AG052328-01A1

Proteomic and glycomic analysis of human brain and lung cancers
07/01/2016 - 06/30/2017 (PI)
University of California, San Francisco NIH NCI
4U01CA168878-05

Thalamic Axonal Pathways and Extracellular Matrix Abnormalities in Schizophrenia
09/01/2015 - 06/30/2017 (PI)
McLean Hospital Corporation NIH NIMH
5R01MH105608-02

Quantitative glycomics and proteomics combined with laser capture microdissection
06/01/2013 - 05/21/2017 (PI)
Agilent Technologies, Inc.

A Thermo-Fisher Scientific Q-Exactive HF Mass Spectrometry System
05/01/2016 - 04/30/2017 (PI)
NIH/Office of the Director
1S10OD021651-01

Extracellular Sulfatases As Novel Biomarkers for Early Detection of Cancer
07/01/2015 - 06/30/2016 (PI)
University of California, San Francisco NIH NCI
5U01CA168878-04

Post-Biosynthetic Remodeling of Heparan Sulfate
01/18/2010 - 12/31/2014 (PI)
NIH/National Heart, Lung, and Blood Institute
5R01HL098950-04

Oligosaccharide Moieties of Human Milk Glycans That Inhibit Pathogenesis
07/01/2010 - 06/30/2013 (PI)
Trustees of Boston College NIH NICHD

Showing 10 of 17 results. Show All Results



Yr Title Project-Sub Proj Pubs
2016 A Thermo-Fisher Scientific Q-Exactive HF Mass Spectrometry System 1S10OD021651-01 1
2016 Software for automated interpretation of heparan sulfate tandem mass spectra 5R21HL131554-02 2
2016 Software for automated interpretation of heparan sulfate tandem mass spectra 3R21HL131554-02S1 2
2016 Quantitative profiling of glycosaminoglycans from breast tumor tissue arrays 5R21CA177479-03 1
2015 Software for automated interpretation of heparan sulfate tandem mass spectra 1R21HL131554-01 2
2014 Quantitative profiling of glycosaminoglycans from breast tumor tissue arrays 1R21CA177479-01A1 1
2014 Quantitative profiling of glycosaminoglycans from breast tumor tissue arrays 1R21CA177479-01A1 1
2013 Post-biosynthetic Remodeling of Heparan Sulfate 5R01HL098950-04 27
2012 Post-biosynthetic Remodeling of Heparan Sulfate 5R01HL098950-03 27
2011 ANALYSIS OF NITROUS ACID DEPOLYMERIZATION PRODUCTS OF GLYCOSAMINOGLYCANS 5P41RR010888-15-5081 236
Showing 10 of 129 results. Show All Results
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

  1. Glaskin RS, Khatri K, Wang Q, Zaia J, Costello CE. Construction of a Database of Collision Cross Section Values for Glycopeptides, Glycans, and Peptides Determined by IM-MS. Anal Chem. 2017 Apr 18; 89(8):4452-4460.View Related Profiles. PMID: 28323417; DOI: 10.1021/acs.analchem.6b04146;.
  2. Wu ZL, Huang X, Ethen C, Tatge T, Pasek M, Zaia J. Non-reducing end labeling of heparan sulfate via click chemistry and a high throughput ELISA assay for heparanase. Glycobiology. 2016 Dec 26. PMID: 28025251; DOI: 10.1093/glycob/cww130;.
  3. Liu Y, McBride R, Stoll M, Palma AS, Silva L, Agravat S, Aoki-Kinoshita KF, Campbell MP, Costello CE, Dell A, Haslam SM, Karlsson NG, Khoo KH, Kolarich D, Novotny MV, Packer NH, Ranzinger R, Rapp E, Rudd PM, Struwe WB, Tiemeyer M, Wells L, York WS, Zaia J, Kettner C, Paulson JC, Feizi T, Smith DF. The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data. Glycobiology. 2016 Nov 22.View Related Profiles. PMID: 27993942.
  4. Zaia J, Khatri K, Klein J, Shao C, Sheng Y, Viner R. Complete Molecular Weight Profiling of Low-Molecular Weight Heparins Using Size Exclusion Chromatography-Ion Suppressor-High-Resolution Mass Spectrometry. Anal Chem. 2016 Nov 01; 88(21):10654-10660.View Related Profiles. PMID: 27709905.
  5. Khatri K, Klein JA, Zaia J. Use of an informed search space maximizes confidence of site-specific assignment of glycoprotein glycosylation. Anal Bioanal Chem. 2017 Jan; 409(2):607-618.View Related Profiles. PMID: 27734143; DOI: 10.1007/s00216-016-9970-5;.
  6. Dierker T, Shao C, Haitina T, Zaia J, Hinas A, Kjellén L. Nematodes join the family of chondroitin sulfate-synthesizing organisms: Identification of an active chondroitin sulfotransferase in Caenorhabditis elegans. Sci Rep. 2016 Oct 05; 6:34662. PMID: 27703236; DOI: 10.1038/srep34662;.
  7. Struwe WB, Agravat S, Aoki-Kinoshita KF, Campbell MP, Costello CE, Dell A, Ten Feizi, Haslam SM, Karlsson NG, Khoo KH, Kolarich D, Liu Y, McBride R, Novotny MV, Packer NH, Paulson JC, Rapp E, Ranzinger R, Rudd PM, Smith DF, Tiemeyer M, Wells L, York WS, Zaia J, Kettner C. The minimum information required for a glycomics experiment (MIRAGE) project: sample preparation guidelines for reliable reporting of glycomics datasets. Glycobiology. 2016 Sep; 26(9):907-910.View Related Profiles. PMID: 27654115.
  8. Sethi MK, Zaia J. Extracellular matrix proteomics in schizophrenia and Alzheimer''s disease. Anal Bioanal Chem. 2017 Jan; 409(2):379-394. PMID: 27601046; DOI: 10.1007/s00216-016-9900-6;.
  9. He H, Liu D, Lin H, Jiang S, Ying Y, Chun S, Deng H, Zaia J, Wen R, Luo Z. Phosphatidylethanolamine binding protein 4 (PEBP4) is a secreted protein and has multiple functions. Biochim Biophys Acta. 2016 Jul; 1863(7 Pt A):1682-9.View Related Profiles. PMID: 27033522; DOI: 10.1016/j.bbamcr.2016.03.022;.
  10. Khatri K, Klein JA, White MR, Grant OC, Leymarie N, Woods RJ, Hartshorn KL, Zaia J. Integrated Omics and Computational Glycobiology Reveal Structural Basis for Influenza A Virus Glycan Microheterogeneity and Host Interactions. Mol Cell Proteomics. 2016 Jun; 15(6):1895-912.View Related Profiles. PMID: 26984886; DOI: 10.1074/mcp.M116.058016;.
Showing 10 of 112 results. Show More

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 112 publications over 28 distinct years, with a maximum of 14 publications in 2013

YearPublications
19851
19872
19911
19921
19943
19951
19963
19971
19983
19992
20002
20014
20021
20032
20042
20051
20066
20072
20084
20093
20109
201112
20128
201314
20149
20154
201610
20171
In addition to these self-described keywords below, a list of MeSH based concepts is available here.

glycomics
glycosaminoglycan
heparan sulfate
heparin
mass spectrometry
proteoglycan
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670 Albany St Biosquare III
Boston MA 02118
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