Mohsan Saeed, PhD
Assistant Professor
Boston University School of Medicine
Dept of Biochemistry

PhD, University of Tokyo
DVM, University of Agriculture Faisalabad
MPhil, Quaid-i-azam Univ

My laboratory investigates the role that viral proteins, particularly viral proteases, play in remodeling host cells and creating a favorable environment for virus replication. To this end, we take a two-pronged approach: employ modern systems biology methods to get a global view of the virus-host interface and then use classical molecular biology and biochemistry techniques to gain deeper mechanistic insights.

The major focus of my laboratory is to identify and characterize host proteins that are cleaved by viral proteases. For this, we use a relatively unbiased approach to label and capture protein N-termini generated by proteolytic cleavage in virus-infected cells. This powerful proteomics (“degradomics”) approach not only identifies the cleaved proteins but also the site of cleavage within a protein. Once the proteins are identified and their cleavage is validated by orthogonal methods, we then ascertain the functional significance of these cleavages in the virus life cycle.

Besides studying host proteins that we have identified from the degradomics analysis of clinically important enteroviruses, we continue to extend this analysis to viruses from other families with the goal to get a global view of cellular pathways commonly targeted or co-opted by diverse viruses. These studies are expected to provide novel insights into cell biology, antiviral defenses, and disease mechanisms. Also, viral proteases are one of the prime targets for antiviral development, and therefore deeper insights into their function will help improve viral therapeutics.

Boston University
National Emerging Infectious Disease Lab

Boston University
Genome Science Institute

Investigating Interferon Antagonists in Delaying Innate Immune responses to SARS-CoV-2
07/09/2021 - 06/30/2026 (Subcontract PI)
Loyola University, Chicago NIH NIAID

Probing Pathomechanisms of Enterovirus D68 Infection
03/01/2020 - 10/31/2022 (PI)
Charles H. Hood Foundation

Mapping Pathogenic Coronavirus Viral-Host Protein Interaction Environments for Target Discovery
08/03/2020 - 01/02/2022 (Multi-PI)
PI: Mohsan Saeed, PhD
Merck, Sharp & Dohme LLC


Yr Title Project-Sub Proj Pubs

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

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  1. Chin CV, Saeed M. Surgical Strikes on Host Defenses: Role of the Viral Protease Activity in Innate Immune Antagonism. Pathogens. 2022 Apr 28; 11(5). PMID: 35631043; PMCID: PMC9145062; DOI: 10.3390/pathogens11050522;
  2. Kenney DJ, O'Connell AK, Turcinovic J, Montanaro P, Hekman RM, Tamura T, Berneshawi AR, Cafiero TR, Al Abdullatif S, Blum B, Goldstein SI, Heller BL, Gertje HP, Bullitt E, Trachtenberg AJ, Chavez E, Nono ET, Morrison C, Tseng AE, Sheikh A, Kurnick S, Grosz K, Bosmann M, Ericsson M, Huber BR, Saeed M, Balazs AB, Francis KP, Klose A, Paragas N, Campbell JD, Connor JH, Emili A, Crossland NA, Ploss A, Douam F. Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infection. Cell Rep. 2022 Apr 19; 39(3):110714.View Related Profiles. PMID: 35421379; PMCID: PMC8977517; DOI: 10.1016/j.celrep.2022.110714;
  3. Serganov AA, Udi Y, Stein ME, Patel V, Fridy PC, Rice CM, Saeed M, Jacobs EY, Chait BT, Rout MP. Proteomic elucidation of the targets and primary functions of the picornavirus 2A protease. J Biol Chem. 2022 Jun; 298(6):101882. PMID: 35367208; PMCID: PMC9168619; DOI: 10.1016/j.jbc.2022.101882;
  4. Carossino M, Kenney D, O'Connell AK, Montanaro P, Tseng AE, Gertje HP, Grosz KA, Ericsson M, Huber BR, Kurnick SA, Subramaniam S, Kirkland TA, Walker JR, Francis KP, Klose AD, Paragas N, Bosmann M, Saeed M, Balasuriya UBR, Douam F, Crossland NA. Fatal Neurodissemination and SARS-CoV-2 Tropism in K18-hACE2 Mice Is Only Partially Dependent on hACE2 Expression. Viruses. 2022 03 05; 14(3).View Related Profiles. PMID: 35336942; PMCID: PMC8955233; DOI: 10.3390/v14030535;
  5. Ren W, Zhu Y, Lan J, Chen H, Wang Y, Shi H, Feng F, Chen DY, Close B, Zhao X, Wu J, Tian B, Yuan Z, Zhou D, Saeed M, Wang X, Zhang R, Ding Q. Susceptibilities of Human ACE2 Genetic Variants in Coronavirus Infection. J Virol. 2022 01 12; 96(1):e0149221. PMID: 34668773; PMCID: PMC8754202; DOI: 10.1128/JVI.01492-21;
  6. Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Fuchs SY, Connor JH, Douam F, Emili A, Saeed M. SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway. J Virol. 2021 09 09; 95(19):e0086221.View Related Profiles. PMID: 34260266; PMCID: PMC8428404; DOI: 10.1128/JVI.00862-21;
  7. Nageswara Rao D, Zephyr J, Henes M, Chan ET, Matthew AN, Hedger AK, Conway HL, Saeed M, Newton A, Petropoulos CJ, Huang W, Kurt Yilmaz N, Schiffer CA, Ali A. Discovery of Quinoxaline-Based P1-P3 Macrocyclic NS3/4A Protease Inhibitors with Potent Activity against Drug-Resistant Hepatitis C Virus Variants. J Med Chem. 2021 08 26; 64(16):11972-11989. PMID: 34405680; PMCID: PMC9228641; DOI: 10.1021/acs.jmedchem.1c00554;
  8. Kim S, Yee E, Miller EA, Hao Y, Tay DMY, Sung KJ, Jia H, Johnson JM, Saeed M, Mace CR, Yüksel Yurt D, Sikes HD. Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins. ACS Appl Mater Interfaces. 2021 Aug 25; 13(33):38990-39002. PMID: 34379400
  9. Bandyopadhyay S, Douglass J, Kapell S, Khan N, Feitosa-Suntheimer F, Klein JA, Temple J, Brown-Culbertson J, Tavares AH, Saeed M, Lau NC. DNA templates with blocked long 3' end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci. G3 (Bethesda). 2021 08 07; 11(8).View Related Profiles. PMID: 33989385; PMCID: PMC8496256; DOI: 10.1093/g3journal/jkab169;
  10. Weingarten-Gabbay S, Klaeger S, Sarkizova S, Pearlman LR, Chen DY, Gallagher KME, Bauer MR, Taylor HB, Dunn WA, Tarr C, Sidney J, Rachimi S, Conway HL, Katsis K, Wang Y, Leistritz-Edwards D, Durkin MR, Tomkins-Tinch CH, Finkel Y, Nachshon A, Gentili M, Rivera KD, Carulli IP, Chea VA, Chandrashekar A, Bozkus CC, Carrington M, Bhardwaj N, Barouch DH, Sette A, Maus MV, Rice CM, Clauser KR, Keskin DB, Pregibon DC, Hacohen N, Carr SA, Abelin JG, Saeed M, Sabeti PC. Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-of-frame ORFs. Cell. 2021 07 22; 184(15):3962-3980.e17. PMID: 34171305; PMCID: PMC8173604; DOI: 10.1016/j.cell.2021.05.046;
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Bar chart showing 50 publications over 15 distinct years, with a maximum of 13 publications in 2020


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