Maria A. Kukuruzinska, PhD
Professor of Molecular & Cell Biology
Boston University Henry M. Goldman School of Dental Medicine
Dept of Molecular & Cell Biology

PhD, Johns Hopkins University



The long term goal of my work is to elucidate the regulatory mechanisms underlying the interactions between the metabolic pathway of protein N-glycosylation and intercellular adhesion in tissue development and disease.

Cross Talk Between Protein N-glycosylation, E-cadherin-mediated Cell-Cell Adhesion and Canonical Wnt Signaling. Studies in my laboratory have unveiled a critical role for N-glycosylation in the function of E-cadherin, a major epithelial cell-cell adhesion receptor that forms adherens junctions (AJs). They have shown that N-glycosylation affects the maturity of AJs and the assembly of tight junctions (TJs), as well as cytoskeletal dynamics. On a molecular level, the N-glycosylation status of E-cadherin is controlled by the DPAGT1 gene, the first gene in the N-glycosylation pathway and its key regulator. At the same time, E-cadherin junctions regulate DPAGT1 expression, indicating the existence of a bidirectional feedback loop between the metabolic pathway of protein N-glycosylation and cell-cell adhesion. Current studies in my laboratory are aimed at elucidating the molecular mechanism via which AJs regulate N-glycosylation.

Mechanisms Underlying Salivary Gland Development. Another major project in my laboratory focuses on the key mechanisms that drive submandibular gland (SMG) development. We have shown that E-cadherin regulates major events during SMG morphogenesis, including proliferation of acinar and ductal progenitors, formation of new buds and survival of ductal progenitors during tubulogenesis. E-cadherin also plays an important role in the planar cell polarity pathway that drives ductal axis extension during SMG morphogenesis. These developmental functions of E-cadherin are regulated by N-glycosylation. Our ongoing studies focus on the molecular characterization of how N-glycosylation and E-cadherin impact acinar and ductal cell fate specification and drive the formation of mature SMG structures.

Molecular Basis of Oral Cancer. The conceptual framework of our mechanistic studies is being applied to investigation of the development and progression of oral cancer. Our recent work has shown that aberrant activation of cellular N-glycosylation promotes the development and progression of oral squamous cell carcinoma (OSCC). Partial inhibition of cellular N-glycosylation in oral cancer cell lines leads to the stabilization of intercellular adhesion, which then drives the mesenchymal to epithelial transition. Current studies examine the molecular basis of over-expression of DPAGT1 in OSCC and its relationship to the downstream signaling pathways that impact E-cadherin’s tumor suppressive function.

Molecular Basis of Sjogren’s Syndrome. Recently, we have initiated studies on Sjogren’s Syndrome (SS), an autoimmune disease that affects salivary and lacrimal glands. Although Sjogren’s disease has long been thought to be caused by lymphocytic infiltration, our recent work has suggested that defective intercellular adhesion is one of the underlying causes of this disease. To expedite the deciphering of the molecular basis of SS and to promote the development of new diagnostics, I co-founded an international collaboration, the Norwegian-United States Initiative on Sjogren’s Syndrome (NUSSIS), that brings together basic researchers and clinicians from the University of Oslo, the University at Albany - SUNY, University of Florida and from the Boston University School of Dental Medicine.

Associate Dean of Research
Boston University Henry M. Goldman School of Dental Medicine
Dean’s Office


Research Assistant Professor of Biochemistry
Boston University School of Medicine
Biochemistry


Mentor for Graduate Medical Students
Boston University School of Medicine, Division of Graduate Medical Sciences


Program Director of Predoctoral Research Program
Boston University Henry M. Goldman School of Dental Medicine




Protocol # VIG-001, Clinical Evaluation of the OncAlert Rapid in Subjects Presenting for Evaluation and/or Initial Biopsy; Impact on Decision-Making.
12/18/2017 - 12/17/2019 (PI)
Vigilant Biosciences, Inc.

Repair, Regeneration and Fibrosis of the Salivary Gland
07/01/2015 - 06/30/2018 (PI)
NIH/National Institute of Dental & Craniofacial Research
5R21DE024954-02

Head and Neck Cancer Symposium: From Pathways to Therapies
08/01/2015 - 07/31/2016 (PI)
NIH/National Institute of Dental & Craniofacial Research
1R13DE025552-01

Defective Cell Polarity and Hippo Signalling in the Etiology of Sjogren's Syndrome
04/01/2014 - 03/31/2016 (PI)
Biogen Idec


Functional Role of the Hippo Pathway in Sjogren's Syndrome
07/01/2012 - 06/30/2014 (PI)
Sjogren's Syndrome Foundation


Regulation of SMG Development by Adhesion Receptors
05/01/2002 - 04/30/2014 (PI)
NIH/National Institute of Dental & Craniofacial Research
5R01DE014437-08

Developmental Expression of the Salivary ALG7 Gene
04/01/1998 - 03/31/2009 (PI)
NIH/National Institute of Dental & Craniofacial Research
5 R01 DE10183 13

Short Term Training: Students in Health Professional Schools
06/01/1998 - 05/31/2003 (PI)
NIH/National Institute of Dental & Craniofacial Research
5 T35 DE07257 05

Role of Cadherins in Salivary Gland Development
07/01/1998 - 06/30/2000 (PI)
Thomas Jefferson University NIH NIDCR




Yr Title Project-Sub Proj Pubs
2016 Repair, Regeneration and Fibrosis of the Salivary Gland 5R21DE024954-02
2015 Head and Neck Cancer Symposium: From Pathways to Therapies 1R13DE025552-01
2013 2013 Salivary Glands and Exocrine Biology Gordon Research Conference 1R13DE023467-01
2012 Regulation of SMG Development by Adhesion Receptors 5R01DE014437-08 3
2011 Regulation of SMG Development by Adhesion Receptors 5R01DE014437-07 3
2010 ROLE OF N-GLYCOSYLATION IN E-CADHERIN MEDIATED CELL-CELL ADHESION 5P41RR010888-14-6809 236
2010 The Role of E-cadherin N-glycans in Oral Cancer 5R01DE015304-05 9
2010 Regulation of SMG Development by Adhesion Receptors 5R01DE014437-06 3
2009 ROLE OF N-GLYCOSYLATION IN E-CADHERIN MEDIATED CELL-CELL ADHESION 5P41RR010888-13-6157 236
2009 The Role of E-cadherin N-glycans in Oral Cancer 5R01DE015304-04 9
Showing 10 of 57 results. Show All Results
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

  1. Alamoud KA, Kukuruzinska MA. Emerging Insights into Wnt/ß-catenin Signaling in Head and Neck Cancer. J Dent Res. 2018 Jun; 97(6):665-673. PMID: 29771197.
     
  2. Alsaqer SF, Tashkandi MM, Kartha VK, Yang YT, Alkheriji Y, Salama A, Varelas X, Kukuruzinska M, Monti S, Bais MV. Inhibition of LSD1 epigenetically attenuates oral cancer growth and metastasis. Oncotarget. 2017 Sep 26; 8(43):73372-73386.View Related Profiles. PMID: 29088714.
     
  3. Bais MV, Kukuruzinska M, Trackman PC. Corrigendum to "Orthotopic non-metastatic and metastatic oral cancer mouse models" [Oral Oncol. 51(5) (2015) 476-482]. Oral Oncol. 2017 09; 72:201.View Related Profiles. PMID: 28743466.
     
  4. Szymaniak AD, Mi R, McCarthy SE, Gower AC, Reynolds TL, Mingueneau M, Kukuruzinska M, Varelas X. The Hippo pathway effector YAP is an essential regulator of ductal progenitor patterning in the mouse submandibular gland. Elife. 2017 May 11; 6.View Related Profiles. PMID: 28492365.
     
  5. Alamoud, K., Kartha, V., Egloff, AM., Sadykov, K., Bais, M., Monti, S., and Kukuruzinska, M.A. IADR/AADR Meeting. Targeting the Wnt/beta-catenin/CBP Axis for the Treatment of Head and Neck Cancer. 2017.
     
  6. Kartha, V., Alamoud, K., Egloff, AM., Sadykov, K., Bais, M., Monti, S., and Kukuruzinska, M.A. 2017 IADR/AADR Meeting. Computational Analyses of Beta-catenin/CBP Inhibition Signature as a Predictor of Treatment Outcome. 2017.
     
  7. Vargas DA, Sun M, Sadykov K, Kukuruzinska MA, Zaman MH. The Integrated Role of Wnt/ß-Catenin, N-Glycosylation, and E-Cadherin-Mediated Adhesion in Network Dynamics. PLoS Comput Biol. 2016 Jul; 12(7):e1005007.View Related Profiles. PMID: 27427963; PMCID: PMC4948889; DOI: 10.1371/journal.pcbi.1005007;.
     
  8. Kartha VK, Stawski L, Han R, Haines P, Gallagher G, Noonan V, Kukuruzinska M, Monti S, Trojanowska M. PDGFRß Is a Novel Marker of Stromal Activation in Oral Squamous Cell Carcinomas. PLoS One. 2016; 11(4):e0154645.View Related Profiles. PMID: 27128408; PMCID: PMC4851360; DOI: 10.1371/journal.pone.0154645;.
     
  9. Stanford EA, Ramirez-Cardenas A, Wang Z, Novikov O, Alamoud K, Koutrakis P, Mizgerd JP, Genco CA, Kukuruzinska M, Monti S, Bais MV, Sherr DH. Role for the Aryl Hydrocarbon Receptor and Diverse Ligands in Oral Squamous Cell Carcinoma Migration and Tumorigenesis. Mol Cancer Res. 2016 Aug; 14(8):696-706.View Related Profiles. PMID: 27130942; PMCID: PMC4987205; DOI: 10.1158/1541-7786.MCR-16-0069;.
     
  10. Rebustini IT, Vlahos M, Packer T, Kukuruzinska MA, Maas RL. An integrated miRNA functional screening and target validation method for organ morphogenesis. Sci Rep. 2016 Mar 16; 6:23215. PMID: 26980315; PMCID: PMC4793243; DOI: 10.1038/srep23215;.
     
Showing 10 of 140 results. Show More

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 140 publications over 33 distinct years, with a maximum of 10 publications in 1999

YearPublications
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75 E. Newton St Evans Building
Boston MA 02118
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