Keywords
Last Name

Olga Gursky, PhD

TitleAssociate Professor
InstitutionBoston University School of Medicine
DepartmentPhysiology & Biophysics
Address700 Albany St Ctr for Adv Biomed Res
Boston MA 02118
Phone(617) 638-7894
ORCID ORCID Icon0000-0002-8598-4824
Other Positions
TitleGraduate Faculty (Primary Mentor of Grad Students)
InstitutionBoston University School of Medicine, Graduate Medical Sciences

 Research Expertise & Professional Interests
Our research focus is on protein folding, structure and stability. It includes the analysis of the energetic-structure-function relationship and folding pathways in proteins and peptides by circular dichroism spectroscopy, differential scanning calorimetry, fluorescence, x-ray crystallography, and site-directed mutagenesis.

Our on-going NIH-funded work is aimed at understanding the energetic and structural basis for the conformational plasticity of apolipoproteins. These are protein constituents of lipoproteins that mediate lipid transport and metabolism and are central in the pathogenesis of atherosclerosis, stroke, and certain forms of amyloidosis. Apolipoproteins are distinct in their structural adaptability to various lipoprotein particles and to plasma. We try to understand in molecular detail the energetic and structural basis for this adaptability.

A major focus of our research is on the molecular mechanisms of lipoprotein stabilization and fusion. In 2002 we revealed that lipoprotein stability is determined by kinetic barriers. Similar barriers may modulate in-vivo lipoprotein transformations. Our goal is to obtain key molecular determinants for these energy barriers.

 Self-Described Keywords
  • calorimetry
  • energy landscapes
  • lipoproteins
  • protein spectroscopy
  • protein stability
  • protein structure-function
  • thermodynamics and kinetics
 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Das M, Mei X, Jayaraman S, Atkinson D, Gursky O. Amyloidogenic mutations in human apolipoprotein A-I are not necessarily destabilizing - a common mechanism of apolipoprotein A-I misfolding in familial amyloidosis and atherosclerosis. FEBS J. 2014 Jun; 281(11):2525-42.
    View in: PubMed
  2. Gursky O. Hot spots in apolipoprotein A-II misfolding and amyloidosis in mice and men. FEBS Lett. 2014 Mar 18; 588(6):845-50.
    View in: PubMed
  3. Gursky O, Jones MK, Mei X, Segrest JP, Atkinson D. Structural basis for distinct functions of the naturally occurring Cys mutants of human apolipoprotein A-I. J Lipid Res. 2013 Dec; 54(12):3244-57.
    View in: PubMed
  4. Lu M, Gursky, O. BioMol. Concepts. Aggregation and fusion of low-density lipoproteins in vivo and in vitro. 2013; bmc-2013-.
  5. Meyers NL, Wang L, Gursky O, Small DM. Changes in helical content or net charge of apolipoprotein C-I alter its affinity for lipid/water interfaces. J Lipid Res. 2013 Jul; 54(7):1927-38.
    View in: PubMed
  6. Gursky O. Crystal structure of ?(185-243)ApoA-I suggests a mechanistic framework for the protein adaptation to the changing lipid load in good cholesterol: from flatland to sphereland via double belt, belt buckle, double hairpin and trefoil/tetrafoil. J Mol Biol. 2013 Jan 9; 425(1):1-16.
    View in: PubMed
  7. Lu M, Gantz DL, Herscovitz H, Gursky O. Kinetic analysis of thermal stability of human low density lipoproteins: a model for LDL fusion in atherogenesis. J Lipid Res. 2012 Oct; 53(10):2175-85.
    View in: PubMed
  8. Gao X, Yuan S, Jayaraman S, Gursky O. Role of apolipoprotein A-II in the structure and remodeling of human high-density lipoprotein (HDL): protein conformational ensemble on HDL. Biochemistry. 2012 Jun 12; 51(23):4633-41.
    View in: PubMed
  9. Jayaraman S, Cavigiolio G, Gursky O. Folded functional lipid-poor apolipoprotein A-I obtained by heating of high-density lipoproteins: relevance to high-density lipoprotein biogenesis. Biochem J. 2012 Mar 15; 442(3):703-12.
    View in: PubMed
  10. Gursky O, Mei X, Atkinson D. The crystal structure of the C-terminal truncated apolipoprotein A-I sheds new light on amyloid formation by the N-terminal fragment. Biochemistry. 2012 Jan 10; 51(1):10-8.
    View in: PubMed
  11. Guha M, Gursky O. Human Plasma Very Low-Density Lipoproteins Are Stabilized by Electrostatic Interactions and Destabilized by Acidic pH. J Lipids. 2011; 2011:493720.
    View in: PubMed
  12. Jayaraman S, Jasuja R, Zakharov MN, Gursky O. Pressure perturbation calorimetry of lipoproteins reveals an endothermic transition without detectable volume changes. Implications for adsorption of apolipoprotein to a phospholipid surface. Biochemistry. 2011 May 17; 50(19):3919-27.
    View in: PubMed
  13. Jayaraman S, Gantz DL, Gursky O. Effects of phospholipase A(2) and its products on structural stability of human LDL: relevance to formation of LDL-derived lipid droplets. J Lipid Res. 2011 Mar; 52(3):549-57.
    View in: PubMed
  14. Guha M, Gursky O. Effects of oxidation on structural stability and remodeling of human very low density lipoprotein. Biochemistry. 2010 Nov 9; 49(44):9584-93.
    View in: PubMed
  15. Klimtchuk ES, Gursky O, Patel RS, Laporte KL, Connors LH, Skinner M, Seldin DC. The critical role of the constant region in thermal stability and aggregation of amyloidogenic immunoglobulin light chain. Biochemistry. 2010 Nov 16; 49(45):9848-57.
    View in: PubMed
  16. Benjwal S, Gursky O. Pressure perturbation calorimetry of apolipoproteins in solution and in model lipoproteins. Proteins. 2010 Apr; 78(5):1175-85.
    View in: PubMed
  17. Jayaraman S, Benjwal S, Gantz DL, Gursky O. Effects of cholesterol on thermal stability of discoidal high density lipoproteins. J Lipid Res. 2010 Feb; 51(2):324-33.
    View in: PubMed
  18. Gao X, Yuan S, Jayaraman S, Gursky O. Differential stability of high-density lipoprotein subclasses: effects of particle size and protein composition. J Mol Biol. 2009 Apr 3; 387(3):628-38.
    View in: PubMed
  19. Guha M, Gao X, Jayaraman S, Gursky O. Correlation of structural stability with functional remodeling of high-density lipoproteins: the importance of being disordered. Biochemistry. 2008 Nov 4; 47(44):11393-7.
    View in: PubMed
  20. Guha M, Gantz DL, Gursky O. Effects of acyl chain length, unsaturation, and pH on thermal stability of model discoidal HDLs. J Lipid Res. 2008 Aug; 49(8):1752-61.
    View in: PubMed
  21. Jayaraman S, Gantz DL, Gursky O. Effects of protein oxidation on the structure and stability of model discoidal high-density lipoproteins. Biochemistry. 2008 Mar 25; 47(12):3875-82.
    View in: PubMed
  22. Gao X, Jayaraman S, Gursky O. Mild oxidation promotes and advanced oxidation impairs remodeling of human high-density lipoprotein in vitro. J Mol Biol. 2008 Feb 29; 376(4):997-1007.
    View in: PubMed
  23. Guha M, England C, Herscovitz H, Gursky O. Thermal transitions in human very-low-density lipoprotein: fusion, rupture, and dissociation of HDL-like particles. Biochemistry. 2007 May 22; 46(20):6043-9.
    View in: PubMed
  24. Jayaraman S, Gantz DL, Gursky O. Effects of oxidation on the structure and stability of human low-density lipoprotein. Biochemistry. 2007 May 15; 46(19):5790-7.
    View in: PubMed
  25. Benjwal S, Jayaraman S, Gursky O. Role of secondary structure in protein-phospholipid surface interactions: reconstitution and denaturation of apolipoprotein C-I:DMPC complexes. Biochemistry. 2007 Apr 3; 46(13):4184-94.
    View in: PubMed
  26. Gursky O. Does alpha-helix folding necessarily provide an energy source for the protein-lipid binding? Protein Pept Lett. 2007; 14(2):171-4.
    View in: PubMed
  27. Jayaraman S, Gantz DL, Gursky O. Effects of salt on the thermal stability of human plasma high-density lipoprotein. Biochemistry. 2006 Apr 11; 45(14):4620-8.
    View in: PubMed
  28. Benjwal S, Verma S, Röhm KH, Gursky O. Monitoring protein aggregation during thermal unfolding in circular dichroism experiments. Protein Sci. 2006 Mar; 15(3):635-9.
    View in: PubMed
  29. Benjwal S, Jayaraman S, Gursky O. Electrostatic effects on the stability of discoidal high-density lipoproteins. Biochemistry. 2005 Aug 2; 44(30):10218-26.
    View in: PubMed
  30. Gursky O. Apolipoprotein structure and dynamics. Curr Opin Lipidol. 2005 Jun; 16(3):287-94.
    View in: PubMed
  31. Chung CM, Chiu JD, Connors LH, Gursky O, Lim A, Dykstra AB, Liepnieks J, Benson MD, Costello CE, Skinner M, Walsh MT. Thermodynamic stability of a kappaI immunoglobulin light chain: relevance to multiple myeloma. Biophys J. 2005 Jun; 88(6):4232-42.
    View in: PubMed
  32. Jayaraman S, Gantz D, Gursky O. Structural basis for thermal stability of human low-density lipoprotein. Biochemistry. 2005 Mar 15; 44(10):3965-71.
    View in: PubMed
  33. Jayaraman S, Gantz DL, Gursky O. Kinetic stabilization and fusion of apolipoprotein A-2:DMPC disks: comparison with apoA-1 and apoC-1. Biophys J. 2005 Apr; 88(4):2907-18.
    View in: PubMed
  34. Jayaraman S, Gantz DL, Gursky O. Poly(ethylene glycol)-induced fusion and destabilization of human plasma high-density lipoproteins. Biochemistry. 2004 May 11; 43(18):5520-31.
    View in: PubMed
  35. Fang Y, Gursky O, Atkinson D. Lipid-binding studies of human apolipoprotein A-I and its terminally truncated mutants. Biochemistry. 2003 Nov 18; 42(45):13260-8.
    View in: PubMed
  36. Fang Y, Gursky O, Atkinson D. Structural studies of N- and C-terminally truncated human apolipoprotein A-I. Biochemistry. 2003 Jun 10; 42(22):6881-90.
    View in: PubMed
  37. Mehta R, Gantz DL, Gursky O. Effects of mutations in apolipoprotein C-1 on the reconstitution and kinetic stability of discoidal lipoproteins. Biochemistry. 2003 Apr 29; 42(16):4751-8.
    View in: PubMed
  38. Mehta R, Gantz DL, Gursky O. Human plasma high-density lipoproteins are stabilized by kinetic factors. J Mol Biol. 2003 Apr 18; 328(1):183-92.
    View in: PubMed
  39. Gursky O. Complex of human apolipoprotein C-1 with phospholipid: thermodynamic or kinetic stability? Biochemistry. 2002 Jun 11; 41(23):7373-84.
    View in: PubMed
  40. Gursky O. Solution conformation of human apolipoprotein C-1 inferred from proline mutagenesis: far- and near-UV CD study. Biochemistry. 2001 Oct 9; 40(40):12178-85.
    View in: PubMed
  41. Gorshkova IN, Liadaki K, Gursky O, Atkinson D, Zannis VI. Probing the lipid-free structure and stability of apolipoprotein A-I by mutation. Biochemistry. 2000 Dec 26; 39(51):15910-9.
    View in: PubMed
  42. Gursky O, Aleshkov S. Temperature-dependent beta-sheet formation in beta-amyloid Abeta(1-40) peptide in water: uncoupling beta-structure folding from aggregation. Biochim Biophys Acta. 2000 Jan 3; 1476(1):93-102.
    View in: PubMed
  43. Gursky O. Probing the conformation of a human apolipoprotein C-1 by amino acid substitutions and trimethylamine-N-oxide. Protein Sci. 1999 Oct; 8(10):2055-64.
    View in: PubMed
  44. Gursky O, Atkinson D. Thermodynamic analysis of human plasma apolipoprotein C-1: high-temperature unfolding and low-temperature oligomer dissociation. Biochemistry. 1998 Feb 3; 37(5):1283-91.
    View in: PubMed
  45. Gursky O, Atkinson D. High- and low-temperature unfolding of human high-density apolipoprotein A-2. Protein Sci. 1996 Sep; 5(9):1874-82.
    View in: PubMed
  46. Gursky O, Atkinson D. Thermal unfolding of human high-density apolipoprotein A-1: implications for a lipid-free molten globular state. Proc Natl Acad Sci U S A. 1996 Apr 2; 93(7):2991-5.
    View in: PubMed
  47. Gursky O, Fontano E, Bhyravbhatla B, Caspar DL. Stereospecific dihaloalkane binding in a pH-sensitive cavity in cubic insulin crystals. Proc Natl Acad Sci U S A. 1994 Dec 20; 91(26):12388-92.
    View in: PubMed
  48. Badger J, Kapulsky A, Gursky O, Bhyravbhatla B, Caspar DL. Structure and selectivity of a monovalent cation binding site in cubic insulin crystals. Biophys J. 1994 Feb; 66(2 Pt 1):286-92.
    View in: PubMed
  49. Gursky O, Badger J, Li Y, Caspar DL. Conformational changes in cubic insulin crystals in the pH range 7-11. Biophys J. 1992 Nov; 63(5):1210-20.
    View in: PubMed
  50. Gursky O, Li Y, Badger J, Caspar DL. Monovalent cation binding to cubic insulin crystals. Biophys J. 1992 Mar; 61(3):604-11.
    View in: PubMed
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