Vassilis I. Zannis, PhD
Professor
Boston University School of Medicine
Dept of Medicine
Cardiovascular Medicine

PhD, University of California, Berkeley



The major focus of our research is on the structure and functions of apoA-I and apoE in relation to the biogenesis of HDL, atherosclerosis and Alzheimer's disease. Using adenovirus-mediated gene transfer in apoA-I-deficient mice, we have established that apoA-I mutations inhibit discrete steps in a pathway that leads to the biogenesis and remodeling of HDL. To this point, five discrete categories of apoA-I mutants have been characterized that may affect the interactions of apoA-I with ABCA1 or LCAT or may influence the plasma PLTP activity or may cause various forms of dyslipidemia. Biogenesis of HDL is not a unique property of apoA-I. Using adenovirus-mediated gene transfer of apoE in apoA-I- or ABCA1-deficient mice, we have established that apoE also participates in a novel pathway of biogenesis of apoE-containing HDL particles. This process requires the functions of the ABCA1 lipid transporter and LCAT and it is promoted by substitution of hydrophobic residues in the 261 to 269 region of apoE by Ala. The apoE-containing HDL particles formed in the circulation may have atheroprotective properties. ApoE-containing HDL may also have important biological functions in the brain that confer protection from Alzheimer’s disease.

Professor
Boston University School of Medicine
Biochemistry


Graduate Faculty (Primary Mentor of Grad Students)
Boston University School of Medicine, Division of Graduate Medical Sciences




Apolipoprotein Variation and Human Disease
05/01/2000 - 04/30/2005 (PI)
NIH/National Heart, Lung, and Blood Institute
5 R01 HL33952 19

Role of ApoE in Cholesterol and Triglyceride Homeostasis
11/01/2001 - 06/30/2004 (PI)
NATO

ApoE Functions Relevant to AD and Gene Transfer to the Brain Using Recombinant Viral Vectors
09/01/2000 - 08/31/2003 (PI)
Alzheimer's Association




Yr Title Project-Sub Proj Pubs
2011 Molecular and Funcitonal Analysis of Human A-I 5R01HL048739-15 47
2010 Functions of apoE in cholesterol and triglyceride homeostasis 5R01HL068216-09 26
2010 Molecular and Funcitonal Analysis of Human A-I 5R01HL048739-14 47
2009 Functions of apoE in cholesterol and triglyceride homeostasis 5R01HL068216-08 26
2009 Molecular and Funcitonal Analysis of Human A-I 5R01HL048739-13 47
2008 INTRACELLULAR MODIFICATIONS OF HUMAN APOLIPOPROTEIN E 5P41RR010888-12-5227 236
2008 Functions of apoE in cholesterol and triglyceride homeostasis 5R01HL068216-07 26
2008 Molecular and Funcitonal Analysis of Human A-I 2R01HL048739-12A2 47
2007 INTRACELLULAR MODIFICATIONS OF HUMAN APOLIPOPROTEIN E 2P41RR010888-11-8048 236
2007 Functions of apoE in cholesterol and triglyceride homeostasis 5R01HL068216-06 26
Showing 10 of 51 results. Show All Results
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

  1. Zannis VI, Su S, Fotakis P. Role of apolipoproteins, ABCA1 and LCAT in the biogenesis of normal and aberrant high density lipoproteins. J Biomed Res. 2017 Nov 04. PMID: 29109329.
  2. Daminelli EN, Fotakis P, Mesquita CH, Maranhão RC, Zannis VI. Tissue Uptake Mechanisms Involved in the Clearance of Non-Protein Nanoparticles that Mimic LDL Composition: A Study with Knockout and Transgenic Mice. Lipids. 2017 Nov 01. PMID: 29094255.
  3. Tamehiro N, Park MH, Hawxhurst V, Nagpal K, Adams ME, Zannis VI, Golenbock DT, Fitzgerald ML. LXR Agonism Upregulates the Macrophage ABCA1/Syntrophin Protein Complex That Can Bind ApoA-I and Stabilized ABCA1 Protein, but Complex Loss Does Not Inhibit Lipid Efflux. Biochemistry. 2015 Nov 24; 54(46):6931-41. PMID: 26506427; PMCID: PMC4874254; DOI: 10.1021/acs.biochem.5b00894;.
  4. Dafnis I, Metso J, Zannis VI, Jauhiainen M, Chroni A. Influence of Isoforms and Carboxyl-Terminal Truncations on the Capacity of Apolipoprotein E To Associate with and Activate Phospholipid Transfer Protein. Biochemistry. 2015 Sep 29; 54(38):5856-66. PMID: 26337529; DOI: 10.1021/acs.biochem.5b00681;.
  5. Tiniakou I, Kanaki Z, Georgopoulos S, Chroni A, Van Eck M, Fotakis P, Zannis VI, Kardassis D. Natural human apoA-I mutations L141RPisa and L159RFIN alter HDL structure and functionality and promote atherosclerosis development in mice. Atherosclerosis. 2015 Nov; 243(1):77-85. PMID: 26363436; DOI: 10.1016/j.atherosclerosis.2015.08.028;.
  6. Fotakis P, Kuivenhoven JA, Dafnis E, Kardassis D, Zannis VI. The Effect of Natural LCAT Mutations on the Biogenesis of HDL. Biochemistry. 2015 Jun 2; 54(21):3348-59. PMID: 25948084; DOI: 10.1021/acs.biochem.5b00180;.
  7. Tiniakou I, Drakos E, Sinatkas V, Van Eck M, Zannis VI, Boumpas D, Verginis P, Kardassis D. High-density lipoprotein attenuates Th1 and th17 autoimmune responses by modulating dendritic cell maturation and function. J Immunol. 2015 May 15; 194(10):4676-87. PMID: 25870241; PMCID: PMC4417411; DOI: 10.4049/jimmunol.1402870;.
  8. Kardassis D, Gafencu A, Zannis VI, Davalos A. Regulation of HDL genes: transcriptional, posttranscriptional, and posttranslational. Handb Exp Pharmacol. 2015; 224:113-79. PMID: 25522987; DOI: 10.1007/978-3-319-09665-0_3;.
  9. Zannis VI, Fotakis P, Koukos G, Kardassis D, Ehnholm C, Jauhiainen M, Chroni A. HDL biogenesis, remodeling, and catabolism. Handb Exp Pharmacol. 2015; 224:53-111. PMID: 25522986; DOI: 10.1007/978-3-319-09665-0_2;.
  10. Fotakis P, Vezeridis A, Dafnis I, Chroni A, Kardassis D, Zannis VI. apoE3[K146N/R147W] acts as a dominant negative apoE form that prevents remnant clearance and inhibits the biogenesis of HDL. J Lipid Res. 2014 Jul; 55(7):1310-23. PMID: 24776540; PMCID: PMC4076092; DOI: 10.1194/jlr.M048348;.
Showing 10 of 184 results. Show More

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 181 publications over 37 distinct years, with a maximum of 9 publications in 1988 and 1991 and 2000 and 2007

YearPublications
19802
19813
19827
19836
19842
19855
19868
19872
19889
19894
19906
19919
19925
19933
19943
19952
19963
19976
19983
19995
20009
20017
20027
20038
20046
20055
20064
20079
20083
20091
20104
20118
20124
20133
20141
20157
20172
In addition to these self-described keywords below, a list of MeSH based concepts is available here.

biochemistry
gene therapy
molecular biology
molecular genetics
Contact for Mentoring:


700 Albany St Ctr for Adv Biomed Res
Boston MA 02118
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