Florian Douam | Profiles RNS

Florian Douam, PhD

Assistant Professor

Boston University Chobanian & Avedisian School of Medicine

Virology, Immunology & Microbiology

PhD, L'École Normale Supérieure de Lyon
BS, Universite De Tours

Websites

	Google Scholar
	ResearchGate
	National Emerging Infectious Diseases Laboratories Faculty

ORCID : 0000-0002-4791-6767

Florian Douam, Ph.D. has a Ph.D. in molecular virology from École normale supérieure de Lyon, France. He previously worked as a postdoctoral research associate in the Ploss Lab, in the department of molecular biology at Princeton University. He currently leads the Douam Lab located at the National Emerging Infectious Disease Laboratories (NEIDL), which investigates how the human immune system as a tissue interacts with specific viral pathogens and ultimately regulates different outcome of infection.

Faculty
Boston University
National Emerging Infectious Disease Lab

Graduate Faculty (Primary Mentor of Grad Students)
Boston University Chobanian & Avedisian School of Medicine, Graduate Medical Sciences

BU Research

Reverse genetic tools and novel models of henipavirus infection
10/28/2022 - 09/30/2025 (Co-Investigator)
PI: Elke Muhlberger, PhD
Bill and Melinda Gates Foundation

CFAR Developmental Award: A humanized mouse model to investigate the impact of HIV persistence and antiretroviral treatment on vaccine-induced immune responses
07/01/2023 - 06/30/2024 (Subcontract PI)
The Miriam Hospital NIH NIAID
2P30AI042853-25

Evaluation of mRNA-based vaccine approaches against DENV
03/14/2023 - 05/30/2024 (Multi-PI)
PI: Florian Douam, PhD
Moderna Therapeutics

Characterization of a human-specific positive regulator of flavivirus infection
04/12/2021 - 03/31/2024 (PI)
NIH/National Institute of Allergy & Infectious Diseases
3K22AI144050-02S1

Defining the Impact of Per/Polyfluoroalkyl Substance Exposure on Susceptibility to SARS-CoV-2 Infection and Disease
03/02/2021 - 02/29/2024 (Multi-PI)
PI: Florian Douam, PhD
NIH/National Institute of Environmental Health Sciences
5R21ES032882-02

Evaluation of mRNA-based vaccine approaches against endemic coronaviruses
12/07/2021 - 09/30/2023 (PI)
Moderna Therapeutics

Therapeutic effects of NAPc2 in SARS-CoV-2 infection of K18-hACE2 transgenic mice
03/15/2021 - 03/31/2022 (Multi-PI)
PI: Florian Douam, PhD
ARCA Biopharma, Inc.

Title

Yr	Title	Project-Sub Proj	Pubs

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis Copy PMIDs To Clipboard

Kenney D, O'Connell AK, Tseng AE, Turcinovic J, Sheehan ML, Nitido AD, Montanaro P, Gertje HP, Ericsson M, Connor JH, Vrbanac V, Crossland NA, Harly C, Balazs AB, Douam F. Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytes. bioRxiv. 2024 Mar 08.View Related Profiles.

Douam, Florian
Connor, John
Crossland, Nicholas

PMID: 38496468; PMCID: PMC10942442; DOI: 10.1101/2024.03.08.583965;

Mesev EV, Lin AE, Guare EG, Heller BL, Douam F, Adamson B, Toettcher JE, Ploss A. Membrane-proximal motifs encode differences in signaling strength between type I and III interferon receptors. Sci Signal. 2023 Oct 10; 16(806):eadf5494. PMID: 37816090; PMCID: PMC10939449; DOI: 10.1126/scisignal.adf5494;

McGee JE, Kirsch JR, Kenney D, Chavez E, Shih TY, Douam F, Wong WW, Grinstaff MW. Complete substitution with modified nucleotides suppresses the early interferon response and increases the potency of self-amplifying RNA. bioRxiv. 2023 Sep 17.View Related Profiles.

Douam, Florian
Grinstaff, Mark

PMID: 37745375; PMCID: PMC10516017; DOI: 10.1101/2023.09.15.557994;

Hogan G, Winer BY, Ahodantin J, Sellau J, Huang T, Douam F, Funaki M, Chiriboga L, Su L, Ploss A. Persistent hepatitis B virus and HIV coinfections in dually humanized mice engrafted with human liver and immune system. J Med Virol. 2023 Jul; 95(7):e28930. PMID: 37403703

Chen DY, Turcinovic J, Feng S, Kenney DJ, Chin CV, Choudhary MC, Conway HL, Semaan M, Close BJ, Tavares AH, Seitz S, Khan N, Kapell S, Crossland NA, Li JZ, Douam F, Baker SC, Connor JH, Saeed M. Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus. iScience. 2023 May 19; 26(5):106634.View Related Profiles.

Douam, Florian
Connor, John
Saeed, Mohsan
Khan, Nazimuddin
Crossland, Nicholas
Seitz, Scott
Kapell, Sebastian

PMID: 37095858; PMCID: PMC10083141; DOI: 10.1016/j.isci.2023.106634;

Chen DY, Chin CV, Kenney D, Tavares AH, Khan N, Conway HL, Liu G, Choudhary MC, Gertje HP, O'Connell AK, Adams S, Kotton DN, Herrmann A, Ensser A, Connor JH, Bosmann M, Li JZ, Gack MU, Baker SC, Kirchdoerfer RN, Kataria Y, Crossland NA, Douam F, Saeed M. Spike and nsp6 are key determinants of SARS-CoV-2 Omicron BA.1 attenuation. Nature. 2023 Mar; 615(7950):143-150.View Related Profiles.

Kotton, Darrell
Douam, Florian
Connor, John
Bosmann, Markus
Saeed, Mohsan
Khan, Nazimuddin
Crossland, Nicholas
Kataria, Yachana

PMID: 36630998

Chen DY, Kenney D, Chin CV, Tavares AH, Khan N, Conway HL, Liu G, Choudhary MC, Gertje HP, O'Connell AK, Kotton DN, Herrmann A, Ensser A, Connor JH, Bosmann M, Li JZ, Gack MU, Baker SC, Kirchdoerfer RN, Kataria Y, Crossland NA, Douam F, Saeed M. Role of spike in the pathogenic and antigenic behavior of SARS-CoV-2 BA.1 Omicron. bioRxiv. 2023 Jan 10.View Related Profiles.

Kotton, Darrell
Douam, Florian
Connor, John
Bosmann, Markus
Saeed, Mohsan
Khan, Nazimuddin
Crossland, Nicholas
Kataria, Yachana

PMID: 36263066; PMCID: PMC9580375; DOI: 10.1101/2022.10.13.512134;

Douam F, Ploss A. A humanized "new-trophil" mouse to study early inflammatory processes. Proc Natl Acad Sci U S A. 2022 Dec 06; 119(49):e2216699119. PMID: 36442110; PMCID: PMC9894160; DOI: 10.1073/pnas.2216699119;

Kenney DJ, O'Connell AK, Turcinovic J, Montanaro P, Hekman RM, Tamura T, Berneshawi AR, Cafiero TR, Al Abdullatif S, Blum B, Goldstein SI, Heller BL, Gertje HP, Bullitt E, Trachtenberg AJ, Chavez E, Nono ET, Morrison C, Tseng AE, Sheikh A, Kurnick S, Grosz K, Bosmann M, Ericsson M, Huber BR, Saeed M, Balazs AB, Francis KP, Klose A, Paragas N, Campbell JD, Connor JH, Emili A, Crossland NA, Ploss A, Douam F. Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infection. Cell Rep. 2022 Apr 19; 39(3):110714.View Related Profiles.

Emili, Andrew
Blum, Benjamin
Huber, Bertrand
Bullitt, Esther
Douam, Florian
Connor, John
Campbell, Joshua
Bosmann, Markus
Saeed, Mohsan
Crossland, Nicholas

PMID: 35421379; PMCID: PMC8977517; DOI: 10.1016/j.celrep.2022.110714;

Ren W, Ju X, Gong M, Lan J, Yu Y, Long Q, Kenney DJ, O'Connell AK, Zhang Y, Zhong J, Zhong G, Douam F, Wang X, Huang A, Zhang R, Ding Q. Characterization of SARS-CoV-2 Variants B.1.617.1 (Kappa), B.1.617.2 (Delta), and B.1.618 by Cell Entry and Immune Evasion. mBio. 2022 Apr 26; 13(2):e0009922. PMID: 35266815; PMCID: PMC9040861; DOI: 10.1128/mbio.00099-22;

Showing 10 of 41 results. Show More

Carossino M, Kenney D, O'Connell AK, Montanaro P, Tseng AE, Gertje HP, Grosz KA, Ericsson M, Huber BR, Kurnick SA, Subramaniam S, Kirkland TA, Walker JR, Francis KP, Klose AD, Paragas N, Bosmann M, Saeed M, Balasuriya UBR, Douam F, Crossland NA. Fatal Neurodissemination and SARS-CoV-2 Tropism in K18-hACE2 Mice Is Only Partially Dependent on hACE2 Expression. Viruses. 2022 03 05; 14(3).View Related Profiles.

Huber, Bertrand
Douam, Florian
Bosmann, Markus
Saeed, Mohsan
Crossland, Nicholas
Subramaniam, Saravanan

PMID: 35336942; PMCID: PMC8955233; DOI: 10.3390/v14030535;

Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Fuchs SY, Connor JH, Douam F, Emili A, Saeed M. SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway. J Virol. 2021 09 09; 95(19):e0086221.View Related Profiles.

Emili, Andrew
Blum, Benjamin
Chen, Christopher
Kotton, Darrell
Douam, Florian
Connor, John
Saeed, Mohsan
Khan, Nazimuddin
Crossland, Nicholas
Goel, Raghuveera
Chitalia, Vipul

PMID: 34260266; PMCID: PMC8428404; DOI: 10.1128/JVI.00862-21;

Yen HL, Valkenburg S, Sia SF, Choy KT, Peiris JSM, Wong KHM, Crossland N, Douam F, Nicholls JM. Cellular tropism of SARS-CoV-2 in the respiratory tract of Syrian hamsters and B6.Cg-Tg(K18-ACE2)2Prlmn/J transgenic mice. Vet Pathol. 2022 Jul; 59(4):639-647.View Related Profiles.

Douam, Florian
Crossland, Nicholas

PMID: 34467820; PMCID: PMC8721337; DOI: 10.1177/03009858211043084;

Sanders DW, Jumper CC, Ackerman PJ, Bracha D, Donlic A, Kim H, Kenney D, Castello-Serrano I, Suzuki S, Tamura T, Tavares AH, Saeed M, Holehouse AS, Ploss A, Levental I, Douam F, Padera RF, Levy BD, Brangwynne CP. SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation. Elife. 2021 04 23; 10.View Related Profiles.

Douam, Florian
Saeed, Mohsan

PMID: 33890572; PMCID: PMC8104966; DOI: 10.7554/eLife.65962;

Ren W, Zhu Y, Wang Y, Shi H, Yu Y, Hu G, Feng F, Zhao X, Lan J, Wu J, Kenney DJ, Douam F, Tong Y, Zhong J, Xie Y, Wang X, Yuan Z, Zhou D, Zhang R, Ding Q. Comparative analysis reveals the species-specific genetic determinants of ACE2 required for SARS-CoV-2 entry. PLoS Pathog. 2021 03; 17(3):e1009392. PMID: 33760889; PMCID: PMC7990223; DOI: 10.1371/journal.ppat.1009392;

Loffredo M, Lucero H, Chen DY, O'Connell A, Bergqvist S, Munawar A, Bandara A, De Graef S, Weeks SD, Douam F, Saeed M, Munawar AH. The in-vitro effect of famotidine on sars-cov-2 proteases and virus replication. Sci Rep. 2021 03 08; 11(1):5433.View Related Profiles.

Douam, Florian
Saeed, Mohsan

PMID: 33686143; PMCID: PMC7940615; DOI: 10.1038/s41598-021-84782-w;

Carossino M, Montanaro P, O'Connell A, Kenney D, Gertje H, Grosz KA, Kurnick SA, Bosmann M, Saeed M, Balasuriya UBR, Douam F, Crossland NA. Fatal neuroinvasion of SARS-CoV-2 in K18-hACE2 mice is partially dependent on hACE2 expression. bioRxiv. 2021 Jan 15.View Related Profiles.

Huber, Bertrand
Douam, Florian
Bosmann, Markus
Saeed, Mohsan
Crossland, Nicholas
Subramaniam, Saravanan

PMID: 33469581; PMCID: PMC7814818; DOI: 10.1101/2021.01.13.425144;

Nimgaonkar I, Archer NF, Becher I, Shahrad M, LeDesma RA, Mateus A, Caballero-Gómez J, Berneshawi AR, Ding Q, Douam F, Gaska JM, Savitski MM, Kim H, Ploss A. Isocotoin suppresses hepatitis E virus replication through inhibition of heat shock protein 90. Antiviral Res. 2021 01; 185:104997. PMID: 33326835; PMCID: PMC8649941; DOI: 10.1016/j.antiviral.2020.104997;

Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Kapell S, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Connor JH, Douam F, Emili A, Saeed M. SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway. bioRxiv. 2020 Oct 28.View Related Profiles.

Emili, Andrew
Blum, Benjamin
Chen, Christopher
Kotton, Darrell
Douam, Florian
Connor, John
Saeed, Mohsan
Khan, Nazimuddin
Crossland, Nicholas
Goel, Raghuveera
Kapell, Sebastian
Chitalia, Vipul

PMID: 33140044; PMCID: PMC7605551; DOI: 10.1101/2020.10.27.358259;

Ramanathan HN, Zhang S, Douam F, Mar KB, Chang J, Yang PL, Schoggins JW, Ploss A, Lindenbach BD. A Sensitive Yellow Fever Virus Entry Reporter Identifies Valosin-Containing Protein (VCP/p97) as an Essential Host Factor for Flavivirus Uncoating. mBio. 2020 04 14; 11(2). PMID: 32291299; PMCID: PMC7157815; DOI: 10.1128/mBio.00467-20;

O'Connell AK, Douam F. Humanized Mice for Live-Attenuated Vaccine Research: From Unmet Potential to New Promises. Vaccines (Basel). 2020 Jan 21; 8(1). PMID: 31973073; PMCID: PMC7157703; DOI: 10.3390/vaccines8010036;

Douam F, Ziegler CGK, Hrebikova G, Fant B, Leach R, Parsons L, Wang W, Gaska JM, Winer BY, Heller B, Shalek AK, Ploss A. Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses. Nat Commun. 2018 11 28; 9(1):5031. PMID: 30487575; PMCID: PMC6262001; DOI: 10.1038/s41467-018-07478-2;

Douam F, Ploss A. Yellow Fever Virus: Knowledge Gaps Impeding the Fight Against an Old Foe. Trends Microbiol. 2018 11; 26(11):913-928. PMID: 29933925; PMCID: PMC6340642; DOI: 10.1016/j.tim.2018.05.012;

Ding Q, Gaska JM, Douam F, Wei L, Kim D, Balev M, Heller B, Ploss A. Species-specific disruption of STING-dependent antiviral cellular defenses by the Zika virus NS2B3 protease. Proc Natl Acad Sci U S A. 2018 07 03; 115(27):E6310-E6318. PMID: 29915078; PMCID: PMC6142274; DOI: 10.1073/pnas.1803406115;

Douam F, Ploss A. The use of humanized mice for studies of viral pathogenesis and immunity. Curr Opin Virol. 2018 04; 29:62-71. PMID: 29604551; PMCID: PMC5940492; DOI: 10.1016/j.coviro.2018.03.003;

Douam F, Fusil F, Enguehard M, Dib L, Nadalin F, Schwaller L, Hrebikova G, Mancip J, Mailly L, Montserret R, Ding Q, Maisse C, Carlot E, Xu K, Verhoeyen E, Baumert TF, Ploss A, Carbone A, Cosset FL, Lavillette D. A protein coevolution method uncovers critical features of the Hepatitis C Virus fusion mechanism. PLoS Pathog. 2018 03; 14(3):e1006908. PMID: 29505618; PMCID: PMC5854445; DOI: 10.1371/journal.ppat.1006908;

Douam F, Soto Albrecht YE, Hrebikova G, Sadimin E, Davidson C, Kotenko SV, Ploss A. Type III Interferon-Mediated Signaling Is Critical for Controlling Live Attenuated Yellow Fever Virus Infection In Vivo. MBio. 2017 08 15; 8(4). PMID: 28811340; PMCID: PMC5559630; DOI: 10.1128/mBio.00819-17;

Douam F, Hrebikova G, Albrecht YE, Sellau J, Sharon Y, Ding Q, Ploss A. Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome. Nat Commun. 2017 03 14; 8:14781. PMID: 28290449; PMCID: PMC5424064; DOI: 10.1038/ncomms14781;

Douam F, Ding Q, Ploss A. Recent advances in understanding hepatitis C. F1000Res. 2016; 5. PMID: 26918166; PMCID: PMC4755394; DOI: 10.12688/f1000research.7354.1;

Douam F, Bobay LM, Maurin G, Fresquet J, Calland N, Maisse C, Durand T, Cosset FL, Féray C, Lavillette D. Specialization of Hepatitis C Virus Envelope Glycoproteins for B Lymphocytes in Chronically Infected Patients. J Virol. 2016 01 15; 90(2):992-1008. PMID: 26537674; PMCID: PMC4702674; DOI: 10.1128/JVI.02516-15;

Douam F, Gaska JM, Winer BY, Ding Q, von Schaewen M, Ploss A. Genetic Dissection of the Host Tropism of Human-Tropic Pathogens. Annu Rev Genet. 2015; 49:21-45. PMID: 26407032; PMCID: PMC5075990; DOI: 10.1146/annurev-genet-112414-054823;

Fusil F, Calattini S, Amirache F, Mancip J, Costa C, Robbins JB, Douam F, Lavillette D, Law M, Defrance T, Verhoeyen E, Cosset FL. A Lentiviral Vector Allowing Physiologically Regulated Membrane-anchored and Secreted Antibody Expression Depending on B-cell Maturation Status. Mol Ther. 2015 Nov; 23(11):1734-1747. PMID: 26281898; PMCID: PMC4817946; DOI: 10.1038/mt.2015.148;

Fénéant L, Potel J, François C, Sané F, Douam F, Belouzard S, Calland N, Vausselin T, Rouillé Y, Descamps V, Baumert TF, Duverlie G, Lavillette D, Hober D, Dubuisson J, Wychowski C, Cocquerel L. New Insights into the Understanding of Hepatitis C Virus Entry and Cell-to-Cell Transmission by Using the Ionophore Monensin A. J Virol. 2015 Aug; 89(16):8346-64. PMID: 26041282; PMCID: PMC4524216; DOI: 10.1128/JVI.00192-15;

Douam F, Ploss A. Proteomic approaches to analyzing hepatitis C virus biology. Proteomics. 2015 Jun; 15(12):2051-65. PMID: 25809442; PMCID: PMC4559851; DOI: 10.1002/pmic.201500009;

Douam F, Lavillette D, Cosset FL. The mechanism of HCV entry into host cells. Prog Mol Biol Transl Sci. 2015; 129:63-107. PMID: 25595801

Cartier F, Marcq I, Douam F, Ossart C, Regnier A, Debuysscher V, Lavillette D, Bouhlal H. The expression of the hepatocyte SLAMF3 (CD229) receptor enhances the hepatitis C virus infection. PLoS One. 2014; 9(6):e99601. PMID: 24927415; PMCID: PMC4057114; DOI: 10.1371/journal.pone.0099601;

Bocchetta S, Maillard P, Yamamoto M, Gondeau C, Douam F, Lebreton S, Lagaye S, Pol S, Helle F, Plengpanich W, Guérin M, Bourgine M, Michel ML, Lavillette D, Roingeard P, le Goff W, Budkowska A. Up-regulation of the ATP-binding cassette transporter A1 inhibits hepatitis C virus infection. PLoS One. 2014; 9(3):e92140. PMID: 24646941; PMCID: PMC3960176; DOI: 10.1371/journal.pone.0092140;

Douam F, Dao Thi VL, Maurin G, Fresquet J, Mompelat D, Zeisel MB, Baumert TF, Cosset FL, Lavillette D. Critical interaction between E1 and E2 glycoproteins determines binding and fusion properties of hepatitis C virus during cell entry. Hepatology. 2014 Mar; 59(3):776-88. PMID: 24038151

de Bossoreille de Ribou S, Douam F, Hamant O, Frohlich MW, Negrutiu I. Plant science and agricultural productivity: why are we hitting the yield ceiling? Plant Sci. 2013 Sep; 210:159-76. PMID: 23849123

Vausselin T, Calland N, Belouzard S, Descamps V, Douam F, Helle F, François C, Lavillette D, Duverlie G, Wahid A, Fénéant L, Cocquerel L, Guérardel Y, Wychowski C, Biot C, Dubuisson J. The antimalarial ferroquine is an inhibitor of hepatitis C virus. Hepatology. 2013 Jul; 58(1):86-97. PMID: 23348596; PMCID: PMC7165689; DOI: 10.1002/hep.26273;

Boo I, teWierik K, Douam F, Lavillette D, Poumbourios P, Drummer HE. Distinct roles in folding, CD81 receptor binding and viral entry for conserved histidine residues of hepatitis C virus glycoprotein E1 and E2. Biochem J. 2012 Apr 01; 443(1):85-94. PMID: 22240035

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 41 publications over 12 distinct years, with a maximum of 6 publications in 2021 and 2023

Bar chart showing 41 publications over 12 distinct years, with a maximum of 6 publications in 2021 and 2023

Year	Publications
2012	1
2013	2
2014	4
2015	5
2016	1
2017	2
2018	5
2020	4
2021	6
2022	4
2023	6
2024	1

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In addition to these self-described keywords below, a list of MeSH based concepts is available here.

Virology
Immunology
Human immune response to viral infection
Human/virus interactions
Viral pathogenesis
Viral immunogenicity
Humanized mouse models
Live-attenuated vaccines

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