Keywords
Last Name

John Bernardo, MD

TitleProfessor
InstitutionBoston University School of Medicine
DepartmentMedicine
DivisionPulmonary, Allergy, Sleep & Critical Care Medicine
Address72 E. Concord St Housman (R)
Boston MA 02118
Phone(617) 638-4860
ORCID ORCID Icon0000-0002-3922-0559
Other Positions
TitleResearch Professor
InstitutionBoston University School of Medicine
DepartmentBiochemistry

TitleActive Staff Privileges
InstitutionBoston Medical Center
DepartmentMedicine

 Research Expertise & Professional Interests
Research interests include:

Basic Science Research: Control of Phagocyte Function and novel applications of Flow Cytometry/kinetic flow imaging.

Translational-Clinical Research: Tuberculosis: diagnostics; treatment; community-based prevention; education; policy

Clinical interests include:
-Tuberculosis
-Allergy
-Clinical/Research Activities:

Dr. Bernardo is Professor of Medicine at Boston University School of Medicine. He attends on the pulmonary consult service and critical care unit at Boston Medical Center. He is active in local and national TB programmatic activities, including: National Tuberculosis Controllers’ Association where he has served as Executive Committee President from 2002 to the present. Dr. Bernardo is also an active member of the Advisory Committee on the Elimination of Tuberculosis (ACET; NTCA) and the Association of Public Health Laboratories Task Force on the Future of TB Laboratory Services. Dr. Bernardo is a co-investigator at the Northeast Tuberculosis Regional Training and Medical Consultation Center (CDC), and a TB Control Officer, Massachusetts Department of Public Health, Div of TB Prevention and Control. He is a Principal Investigator at the Boston University site, TB Trials Consortium and co-Principal Investigator, Massachusetts DPH site, TB Epidemiologic Studies Consortium;.

Outside of Boston Medical Center, Dr. Bernardo is a Pulmonary/TB Physician for Boston’s Health Care for the Homeless, Inc., with a weekly, full-service, evening TB Clinic at Pine Street Inn shelter, where Claire Murphy, RN, NP, and Dr Bernardo provide specialty care to persons with TB infection and disease.

Dr. Bernardo’s basic research interests include basic studies of the control of the phagovacuolar environment (human neutrophils and monomuclear phagocytes) using kinetic flow cytometry and novel flow cytometric techniques (NHLBI support), in collaboration with Elizabeth Simons, Ph.D., Professor of Biochemistry. For more details, please see our webpage of TB/HIV Basic Science Research.

 NIH RePORTER Grants

Yr Title Project-Sub Proj Pubs
2000 TUBERCULOSIS TRAINING PROGRAM--BOSTON 5K07HL003035-05 3
1999 TUBERCULOSIS TRAINING PROGRAM--BOSTON 5K07HL003035-04 3
1998 TUBERCULOSIS TRAINING PROGRAM--BOSTON 5K07HL003035-03 3
1997 TUBERCULOSIS TRAINING PROGRAM--BOSTON 5K07HL003035-02 3
1996 TUBERCULOSIS TRAINING PROGRAM--BOSTON 1K07HL003035-01A2 3


 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Chiswick EL, Mella JR, Bernardo J, Remick DG. Acute-Phase Deaths from Murine Polymicrobial Sepsis Are Characterized by Innate Immune Suppression Rather Than Exhaustion. J Immunol. 2015 Oct 15; 195(8):3793-802. PMID: 26371253.
    View in: PubMed
  2. Pollock NR, McAdam AJ, Pai M, Nardell EA, Bernardo J, Banaei N, Mobo J. Interferon ?-release assays for diagnosis of latent tuberculosis in healthcare workers in low-incidence settings: pros and cons. Clin Chem. 2014 May; 60(5):714-8. PMID: 24100806.
    View in: PubMed
  3. Marks SM, Cronin W, Venkatappa T, Maltas G, Chon S, Sharnprapai S, Gaeddert M, Tapia J, Dorman SE, Etkind S, Crosby C, Blumberg HM, Bernardo J. The health-system benefits and cost-effectiveness of using Mycobacterium tuberculosis direct nucleic acid amplification testing to diagnose tuberculosis disease in the United States. Clin Infect Dis. 2013 Aug; 57(4):532-42. PMID: 23697743.
    View in: PubMed
  4. Mancuso JD, Bernardo J, Mazurek GH. The elusive "gold" standard for detecting Mycobacterium tuberculosis infection. Am J Respir Crit Care Med. 2013 Jan 15; 187(2):122-4. PMID: 23322793.
    View in: PubMed
  5. Han LL, Elvin P, Bernardo J. Nonclinical selection criteria for maximizing yield of nucleic acid amplification tests in tuberculosis diagnosis. J Clin Microbiol. 2012 Aug; 50(8):2592-5. PMID: 22622440.
    View in: PubMed
  6. Kellar KL, Gehrke J, Weis SE, Mahmutovic-Mayhew A, Davila B, Zajdowicz MJ, Scarborough R, LoBue PA, Lardizabal AA, Daley CL, Reves RR, Bernardo J, Campbell BH, Whitworth WC, Mazurek GH. Multiple cytokines are released when blood from patients with tuberculosis is stimulated with Mycobacterium tuberculosis antigens. PLoS One. 2011; 6(11):e26545. PMID: 22132075.
    View in: PubMed
  7. Livingston KA, Lobato MN, Sosa LE, Budnick GE, Bernardo J. Mycobacterium tuberculosis testing practices in hospital, commercial and state laboratories in the New England states. Int J Tuberc Lung Dis. 2011 Sep; 15(9):1218-22, i. PMID: 21943849.
    View in: PubMed
  8. Tschampl C, Bernardo J, Garvey T, Garnick D. The need for performance measures on testing for latent tuberculosis infection in primary care. Jt Comm J Qual Patient Saf. 2011 Jul; 37(7):309-16. PMID: 21819029.
    View in: PubMed
  9. Powell RD, Whitworth WC, Bernardo J, Moonan PK, Mazurek GH. Unusual interferon gamma measurements with QuantiFERON-TB Gold and QuantiFERON-TB Gold In-Tube tests. PLoS One. 2011; 6(6):e20061. PMID: 21687702.
    View in: PubMed
  10. Simons ER, Bernardo J. Multiparameter flow cytometric kinetics of phagocyte stimulus responses. Cytometry A. 2011 Jul; 79(7):493-5. PMID: 21656663.
    View in: PubMed
  11. Bernardo J, Long HJ, Simons ER. Initial cytoplasmic and phagosomal consequences of human neutrophil exposure to Staphylococcus epidermidis. Cytometry A. 2010 Mar; 77(3):243-52. PMID: 19937952.
    View in: PubMed
  12. Bernardo J, Yew WW. How are we creating fluoroquinolone-resistant tuberculosis? Am J Respir Crit Care Med. 2009 Aug 15; 180(4):288-9. PMID: 19661251.
    View in: PubMed
  13. Dorman SE, Johnson JL, Goldberg S, Muzanye G, Padayatchi N, Bozeman L, Heilig CM, Bernardo J, Choudhri S, Grosset JH, Guy E, Guyadeen P, Leus MC, Maltas G, Menzies D, Nuermberger EL, Villarino M, Vernon A, Chaisson RE. Substitution of moxifloxacin for isoniazid during intensive phase treatment of pulmonary tuberculosis. Am J Respir Crit Care Med. 2009 Aug 1; 180(3):273-80. PMID: 19406981.
    View in: PubMed
  14. Mazurek GH, Weis SE, Moonan PK, Daley CL, Bernardo J, Lardizabal AA, Reves RR, Toney SR, Daniels LJ, LoBue PA. Prospective comparison of the tuberculin skin test and 2 whole-blood interferon-gamma release assays in persons with suspected tuberculosis. Clin Infect Dis. 2007 Oct 1; 45(7):837-45. PMID: 17806047.
    View in: PubMed
  15. Herrmann JM, Bernardo J, Long HJ, Seetoo K, McMenamin ME, Batista EL, Van Dyke TE, Simons ER. Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils. Infect Immun. 2007 Aug; 75(8):3989-98. PMID: 17526745.
    View in: PubMed
  16. Saukkonen JJ, Cohn DL, Jasmer RM, Schenker S, Jereb JA, Nolan CM, Peloquin CA, Gordin FM, Nunes D, Strader DB, Bernardo J, Venkataramanan R, Sterling TR. An official ATS statement: hepatotoxicity of antituberculosis therapy. Am J Respir Crit Care Med. 2006 Oct 15; 174(8):935-52. PMID: 17021358.
    View in: PubMed
  17. Shieh FK, Snyder G, Horsburgh CR, Bernardo J, Murphy C, Saukkonen JJ. Predicting non-completion of treatment for latent tuberculous infection: a prospective survey. Am J Respir Crit Care Med. 2006 Sep 15; 174(6):717-21. PMID: 16809632.
    View in: PubMed
  18. Herrmann JM, Kantarci A, Long H, Bernardo J, Hasturk H, Wray LV, Simons ER, Van Dyke TE. Simultaneous measurements of cytoplasmic Ca2+ responses and intracellular pH in neutrophils of localized aggressive periodontitis (LAP) patients. J Leukoc Biol. 2005 Sep; 78(3):612-9. PMID: 15937144.
    View in: PubMed
  19. Raffetto JD, Bernardo J, Menzoían JO. Aortobifemoral graft infection with Mycobacterium tuberculosis: treatment with abscess drainage, debridement, and long-term administration of antibiotic agents. J Vasc Surg. 2004 Oct; 40(4):826-9. PMID: 15472616.
    View in: PubMed
  20. Lerman SJ, Bernardo J, Daly JS, Husson R. Tuberculosis prevention in college students. J Am Coll Health. 2004 Sep-Oct; 53(2):53-8. PMID: 15495880.
    View in: PubMed
  21. Jasmer RM, Snyder DC, Saukkonen JJ, Hopewell PC, Bernardo J, King MD, Kawamura LM, Daley CL. Short-course rifampin and pyrazinamide compared with isoniazid for latent tuberculosis infection: a cost-effectiveness analysis based on a multicenter clinical trial. Clin Infect Dis. 2004 Feb 1; 38(3):363-9. PMID: 14727206.
    View in: PubMed
  22. Hsu K, Christiansen D, O'Connor D, Bernardo J, Hacker K. Self-assessment of tuberculosis infection risk by urban adolescents. Arch Pediatr Adolesc Med. 2003 Dec; 157(12):1227-31. PMID: 14662580.
    View in: PubMed
  23. Burman W, Breese P, Weis S, Bock N, Bernardo J, Vernon A. The effects of local review on informed consent documents from a multicenter clinical trials consortium. Control Clin Trials. 2003 Jun; 24(3):245-55. PMID: 12757991.
    View in: PubMed
  24. Mazurek GH, LoBue PA, Daley CL, Bernardo J, Lardizabal AA, Iademarco MF. Detection of Mycobacterium tuberculosis infection by whole-blood interferon-gamma release assay. Clin Infect Dis. 2003 May 1; 36(9):1207-8; author reply 1209-10. PMID: 12715323.
    View in: PubMed
  25. Bernardo J, Hartlaub H, Yu X, Long H, Simons ER. Immune complex stimulation of human neutrophils involves a novel Ca2+/H+ exchanger that participates in the regulation of cytoplasmic pH: flow cytometric analysis of Ca2+/pH responses by subpopulations. J Leukoc Biol. 2002 Dec; 72(6):1172-9. PMID: 12488499.
    View in: PubMed
  26. Jasmer RM, Saukkonen JJ, Blumberg HM, Daley CL, Bernardo J, Vittinghoff E, King MD, Kawamura LM, Hopewell PC. Short-course rifampin and pyrazinamide compared with isoniazid for latent tuberculosis infection: a multicenter clinical trial. Ann Intern Med. 2002 Oct 15; 137(8):640-7. PMID: 12379063.
    View in: PubMed
  27. Panlilio AL, Burwen DR, Curtis AB, Srivastava PU, Bernardo J, Catalano MT, Mendelson MH, Nicholas P, Pagano W, Sulis C, Onorato IM, Chamberland ME. Tuberculin skin testing surveillance of health care personnel. Clin Infect Dis. 2002 Aug 1; 35(3):219-27. PMID: 12115085.
    View in: PubMed
  28. Mazurek GH, LoBue PA, Daley CL, Bernardo J, Lardizabal AA, Bishai WR, Iademarco MF, Rothel JS. Comparison of a whole-blood interferon gamma assay with tuberculin skin testing for detecting latent Mycobacterium tuberculosis infection. JAMA. 2001 Oct 10; 286(14):1740-7. PMID: 11594899.
    View in: PubMed
  29. Bernardo J, Billingslea AM, Blumenthal RL, Seetoo KF, Simons ER, Fenton MJ. Differential responses of human mononuclear phagocytes to mycobacterial lipoarabinomannans: role of CD14 and the mannose receptor. Infect Immun. 1998 Jan; 66(1):28-35. PMID: 9423835.
    View in: PubMed
  30. Bernardo J, Billingslea AM, Ortiz MF, Seetoo KF, Macauley J, Simons ER. Adherence-dependent calcium signaling in monocytes: induction of a CD14-high phenotype, stimulus-responsive subpopulation. J Immunol Methods. 1997 Dec 1; 209(2):165-75. PMID: 9461332.
    View in: PubMed
  31. Crimlisk JT, Bernardo J, Blansfield JS, Loughlin M, McGonagle EG, McEachern G, Roeber J, Farber HW. Endotracheal reintubation: a closer look at a preventable condition. Clin Nurse Spec. 1997 Jul; 11(4):145-50; quiz 151-2. PMID: 9274152.
    View in: PubMed
  32. Polesky A, Farber HW, Gottlieb DJ, Park H, Levinson S, O'Connell JJ, McInnis B, Nieves RL, Bernardo J. Rifampin preventive therapy for tuberculosis in Boston's homeless. Am J Respir Crit Care Med. 1996 Nov; 154(5):1473-7. PMID: 8912767.
    View in: PubMed
  33. Strohmeier GR, Brunkhorst BA, Seetoo KF, Meshulam T, Bernardo J, Simons ER. Role of the Fc gamma R subclasses Fc gamma RII and Fc gamma RIII in the activation of human neutrophils by low and high valency immune complexes. J Leukoc Biol. 1995 Oct; 58(4):415-22. PMID: 7561517.
    View in: PubMed
  34. Strohmeier GR, Brunkhorst BA, Seetoo KF, Bernardo J, Weil GJ, Simons ER. Neutrophil functional responses depend on immune complex valency. J Leukoc Biol. 1995 Oct; 58(4):403-14. PMID: 7561516.
    View in: PubMed
  35. Center DM, Kornfeld H, Wu MJ, Falvo M, Theodore AC, Bernardo J, Berman JS, Cruikshank WW, Djukanovic R, Teran L, et al. Cytokine binding to CD4+ inflammatory cells: implications for asthma. Am J Respir Crit Care Med. 1994 Nov; 150(5 Pt 2):S59-62. PMID: 7952594.
    View in: PubMed
  36. Bernardo J, Brennan L, Brink HF, Ortiz MF, Newburger PE, Simons ER. Chemotactic peptide-induced cytoplasmic pH changes in incubated human monocytes. J Leukoc Biol. 1993 Jun; 53(6):673-8. PMID: 8315350.
    View in: PubMed
  37. Meshulam T, Herscovitz H, Casavant D, Bernardo J, Roman R, Haugland RP, Strohmeier GS, Diamond RD, Simons ER. Flow cytometric kinetic measurements of neutrophil phospholipase A activation. J Biol Chem. 1992 Oct 25; 267(30):21465-70. PMID: 1400459.
    View in: PubMed
  38. Bernardo J. Tuberculosis: a disease of the 1990s. Hosp Pract (Off Ed). 1991 Oct 15; 26(10):195-8, 202, 207-8 passim. PMID: 1918197.
    View in: PubMed
  39. Joyce-Brady M, Rubins JB, Panchenko MP, Bernardo J, Steele MP, Kolm L, Simons ER, Dickey BF. Mechanisms of mastoparan-stimulated surfactant secretion from isolated pulmonary alveolar type 2 cells. J Biol Chem. 1991 Apr 15; 266(11):6859-65. PMID: 1849893.
    View in: PubMed
  40. Davies TA, Bernardo J, Lazzari K, Brennan L., Simons ER. Cytosolic calcium determination: A fluorimetric technique. J Nutr Biochem. 1991; 2:102-106.
  41. Berman JS, Beer DJ, Theodore AC, Kornfeld H, Bernardo J, Center DM. Lymphocyte recruitment to the lung. Am Rev Respir Dis. 1990 Jul; 142(1):238-57. PMID: 2195933.
    View in: PubMed
  42. Bernardo J, Newburger PE, Brennan L, Brink HF, Bresnick SA, Weil G, Simons ER. Simultaneous flow cytometric measurements of cytoplasmic Ca++ and membrane potential changes upon FMLP exposure as HL-60 cells mature into granulocytes: using [Ca++]in as an indicator of granulocyte maturity. J Leukoc Biol. 1990 Mar; 47(3):265-74. PMID: 2307907.
    View in: PubMed
  43. Oh SK, Very DL, Ettinger R, Walker J, Giampaolo C, Bernardo J. Monoclonal antibody to SER immune suppressor detects polymeric forms of haptoglobin. Hybridoma. 1989 Aug; 8(4):449-66. PMID: 2777276.
    View in: PubMed
  44. Berman JS, Cruikshank WW, Beer DJ, Kornfeld H, Bernardo J, Theodore AC, Center DM. Lymphocyte motility and lymphocyte chemoattractant factors. Immunol Invest. 1988 Nov-Dec; 17(8-9):625-77. PMID: 3068125.
    View in: PubMed
  45. Bernardo J, Brink HF, Simons ER. Time dependence of transmembrane potential changes and intracellular calcium flux in stimulated human monocytes. J Cell Physiol. 1988 Jan; 134(1):131-6. PMID: 2826501.
    View in: PubMed
  46. Cruikshank WW, Berman JS, Theodore AC, Bernardo J, Center DM. Lymphokine activation of T4+ T lymphocytes and monocytes. J Immunol. 1987 Jun 1; 138(11):3817-23. PMID: 3108375.
    View in: PubMed
  47. Kaelin RM, Center DM, Grant MM, Bernardo J. Production of lymphocyte chemokinetic activity by stimulated alveolar macrophages. Exp Lung Res. 1986; 10(2):171-86. PMID: 3082619.
    View in: PubMed
  48. Berman JS, Beer DJ, Bernardo J, Kornfeld H, Center DM. A proposed model for the accumulation of helper/inducer lymphocytes in sarcoidosis. The role of lymphocyte subset-specific chemoattractant lymphokines. Ann N Y Acad Sci. 1986; 465:98-109. PMID: 2942081.
    View in: PubMed
  49. Kaelin RM, Semerjian A, Center DM, Bernardo J. Influence of ethanol on human T-lymphocyte migration. J Lab Clin Med. 1984 Nov; 104(5):752-60. PMID: 6092500.
    View in: PubMed
  50. Glassroth JL, Bernardo J, Lucey EC, Center DM, Jung-Legg YJ, Snider GL. Interstitial pulmonary fibrosis induced in hamsters by intratracheally administered chrysotile asbestos. Histology, lung mechanics, and inflammatory events. Am Rev Respir Dis. 1984 Aug; 130(2):242-8. PMID: 6087700.
    View in: PubMed
  51. Kaelin RM, Center DM, Bernardo J, Grant M, Snider GL. The role of macrophage-derived chemoattractant activities in the early inflammatory events of bleomycin-induced pulmonary injury. Am Rev Respir Dis. 1983 Jul; 128(1):132-7. PMID: 6191602.
    View in: PubMed
  52. Bernardo J, Center DM. Hypersensitivity pneumonia. Dis Mon. 1981 May; 27(8):1-64. PMID: 6909070.
    View in: PubMed
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