Horacio Frydman | Profiles RNS

Horacio M. Frydman, PhD

Associate Professor

Boston University College of Arts and Sciences

Biology

PhD, Johns Hopkins University
MSc, Universidade de São Paulo

Websites

	Biology
	National Emerging Infectious Diseases Laboratories
	LinkedIn
	ResearchGate
	Google Scholar

ORCID : 0000-0003-0191-7948

My research generally focuses on understanding how microorganisms and their hosts interact at different biological levels (e.g., molecular, cellular, genetic, ecological, and evolutionary). Specifically, I am interested in the interactions of the intracellular bacteria Wolbachia with their host cells. The infection of invertebrates by Wolbachia represents one of the great pandemics on this planet. Even though Wolbachia is one of the most abundant intracellular bacteria on earth, infecting up to 70% of arthropods and filarial nematodes, their mechanisms of transmission are poorly understood.

Beyond fundamental questions of host-microbe interactions, understanding Wolbachia biology also has specific medical relevance. Wolbachia provides new approaches to treat human and animal filariasis—devastating diseases including river blindness and elephantiasis—caused by parasitic worms. Wolbachia is also a potential agent to control insect vectors that transmit diseases such as dengue, filariasis, and malaria.

The broad aim of my laboratory is to identify the mechanisms required for maintenance of Wolbachia infection through successive generations of their host (vertical transmission) and for infection into new hosts (horizontal or infectious transmission). My work demonstrates that Wolbachia preferentially populate the stem cell niche, the region of the fly ovary containing the stem cells. Tropism for the stem cell niche provides a previously undetected route to reach the germ line. The targeting of stem cell niche by Wolbachia may facilitate their horizontal and vertical transmission. Our main focus is to understand the mechanisms of Wolbachia targeting of the stem cell niche and how it relates to their successful transmission.

Member
Boston University
Evans Center for Interdisciplinary Biomedical Research

Member
Boston University
Genome Science Institute

Faculty
Boston University
National Emerging Infectious Disease Lab

BU Research
NIH RePORTER Research

Pew Fellowship for Heverton Dutra
08/30/2018 - 05/31/2019 (Key Person / Mentor)
The Pew Charitable Trusts

Mechanisms of Wolbachia Stem Cell Niche Tropism
05/15/2013 - 02/28/2018 (PI)
National Science Foundation
IOS-1258127

Manipulation of Stem Cell Activity and Cell Death by Intracellular Bacteria
08/07/2013 - 07/31/2015 (PI)
NIH/National Institute of Allergy & Infectious Diseases
1R56AI097589-01

Title

Yr	Title	Project-Sub Proj	Pubs
2013	Manipulation of stem cell activity and cell death by intracellular bacteria	1R56AI097589-01A1	2
2011	Vector Transmitted Infectious Disease Core	3UC7AI070088-05S1-7213	1
2010	Host-pathogen interactions in targeting Wolbachia to the stem cell niche	5K22AI074909-02	2
2009	Host-pathogen interactions in targeting Wolbachia to the stem cell niche	1K22AI074909-01A1	2

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis Copy PMIDs To Clipboard

Jo C, Bernstein DB, Vaisman N, Frydman HM, Segrè D. Construction and Modeling of a Coculture Microplate for Real-Time Measurement of Microbial Interactions. mSystems. 2023 Apr 27; 8(2):e0001721. PMID: 36802169; PMCID: PMC10134821; DOI: 10.1128/msystems.00017-21;

Deehan M, Lin W, Blum B, Emili A, Frydman H. Intracellular Density of Wolbachia Is Mediated by Host Autophagy and the Bacterial Cytoplasmic Incompatibility Gene cifB in a Cell Type-Dependent Manner in Drosophila melanogaster. mBio. 2021 01 12; 12(1).View Related Profiles.

Emili, Andrew
Blum, Benjamin
Frydman, Horacio
Lin, Weiwei

PMID: 33436431; PMCID: PMC7844536; DOI: 10.1128/mBio.02205-20;

Carneiro Dutra HL, Deehan MA, Frydman H. Wolbachia and Sirtuin-4 interaction is associated with alterations in host glucose metabolism and bacterial titer. PLoS Pathog. 2020 10; 16(10):e1008996. PMID: 33048997; PMCID: PMC7584242; DOI: 10.1371/journal.ppat.1008996;

Lefoulon E, Vaisman N, Frydman HM, Sun L, Voland L, Foster JM, Slatko BE. Author Correction: Large Enriched Fragment Targeted Sequencing (LEFT-SEQ) Applied to Capture of Wolbachia Genomes. Sci Rep. 2019 Dec 24; 9(1):20184. PMID: 31874958; PMCID: PMC6930218; DOI: 10.1038/s41598-019-55305-5;

Lefoulon E, Vaisman N, Frydman HM, Sun L, Voland L, Foster JM, Slatko BE. Large Enriched Fragment Targeted Sequencing (LEFT-SEQ) Applied to Capture of Wolbachia Genomes. Sci Rep. 2019 04 11; 9(1):5939. PMID: 30976027; PMCID: PMC6459864; DOI: 10.1038/s41598-019-42454-w;

Schultz MJ, Tan AL, Gray CN, Isern S, Michael SF, Frydman HM, Connor JH. Wolbachia wStri Blocks Zika Virus Growth at Two Independent Stages of Viral Replication. MBio. 2018 05 22; 9(3).View Related Profiles.

Frydman, Horacio
Connor, John

PMID: 29789369; PMCID: PMC5964347; DOI: 10.1128/mBio.00738-18;

Schultz MJ, Frydman HM, Connor JH. Dual Insect specific virus infection limits Arbovirus replication in Aedes mosquito cells. Virology. 2018 05; 518:406-413.View Related Profiles.

Frydman, Horacio
Connor, John

PMID: 29625404; PMCID: PMC8005325; DOI: 10.1016/j.virol.2018.03.022;

Kamath AD, Deehan MA, Frydman HM. Polar cell fate stimulates Wolbachia intracellular growth. Development. 2018 03 23; 145(6). PMID: 29467241; PMCID: PMC5897597; DOI: 10.1242/dev.158097;

Schultz MJ, Connor JH, Frydman HM. Group B Wolbachia Strain-Dependent Inhibition of Arboviruses. DNA Cell Biol. 2018 Jan; 37(1):2-6.View Related Profiles.

Frydman, Horacio
Connor, John

PMID: 29297702; PMCID: PMC5770089; DOI: 10.1089/dna.2017.4025;

Simhadri RK, Fast EM, Guo R, Schultz MJ, Vaisman N, Ortiz L, Bybee J, Slatko BE, Frydman HM. The Gut Commensal Microbiome of Drosophila melanogaster Is Modified by the Endosymbiont Wolbachia. mSphere. 2017 Sep-Oct; 2(5). PMID: 28932814; PMCID: PMC5597968; DOI: 10.1128/mSphere.00287-17;

Showing 10 of 23 results. Show More

Schultz MJ, Isern S, Michael SF, Corley RB, Connor JH, Frydman HM. Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures. J Virol. 2017 Jul 15; 91(14).View Related Profiles.

Frydman, Horacio
Connor, John
Corley, Ronald

PMID: 28446677; PMCID: PMC5487579; DOI: 10.1128/JVI.00339-17;

Toomey ME, Frydman HM. Extreme divergence of Wolbachia tropism for the stem-cell-niche in the Drosophila testis. PLoS Pathog. 2014 Dec; 10(12):e1004577. PMID: 25521619; PMCID: PMC4270793; DOI: 10.1371/journal.ppat.1004577;

Toomey ME, Panaram K, Fast EM, Beatty C, Frydman HM. Evolutionarily conserved Wolbachia-encoded factors control pattern of stem-cell niche tropism in Drosophila ovaries and favor infection. Proc Natl Acad Sci U S A. 2013 Jun 25; 110(26):10788-93. PMID: 23744038; PMCID: PMC3696799; DOI: 10.1073/pnas.1301524110;

Fast EM, Toomey ME, Panaram K, Desjardins D, Kolaczyk ED, Frydman HM. Wolbachia enhance Drosophila stem cell proliferation and target the germline stem cell niche. Science. 2011 Nov 18; 334(6058):990-2. PMID: 22021671; PMCID: PMC4030408; DOI: 10.1126/science.1209609;

Frydman H. Wolbachia bacterial infection in Drosophila. J Vis Exp. 2007 Feb 25; (2):158. PMID: 18830425; PMCID: PMC2532937; DOI: 10.3791/158;

Beaucher M, Goodliffe J, Hersperger E, Trunova S, Frydman H, Shearn A. Drosophila brain tumor metastases express both neuronal and glial cell type markers. Dev Biol. 2007 Jan 01; 301(1):287-97. PMID: 17055475; PMCID: PMC1859848; DOI: 10.1016/j.ydbio.2006.09.019;

Frydman HM, Li JM, Robson DN, Wieschaus E. Somatic stem cell niche tropism in Wolbachia. Nature. 2006 May 25; 441(7092):509-12. PMID: 16724067; DOI: 10.1038/nature04756;

Frydman HM. Isolation of live bacteria from adult insects. Nature Protocols. 2006.

Ferree PM, Frydman HM, Li JM, Cao J, Wieschaus E, Sullivan W. Wolbachia utilizes host microtubules and Dynein for anterior localization in the Drosophila oocyte. PLoS Pathog. 2005 Oct; 1(2):e14. PMID: 16228015; PMCID: PMC1253842; DOI: 10.1371/journal.ppat.0010014;

Frydman HM, Spradling AC. The receptor-like tyrosine phosphatase lar is required for epithelial planar polarity and for axis determination within drosophila ovarian follicles. Development. 2001 Aug; 128(16):3209-20. PMID: 11688569

Frydman HM, Cadavid EO, Yokosawa J, Henrique Silva F, Navarro-Cattapan LD, Santelli RV, Jacobs-Lorena M, Graessmann M, Graessmann A, Stocker AJ, et al. Molecular characterization of the DNA puff C-8 gene of Rhynchosciara americana. J Mol Biol. 1993 Oct 20; 233(4):799-803. PMID: 8411183; DOI: 10.1006/jmbi.1993.1556;

Soares G, Frydman HM, Cattapan LDN, Santelli RV . Studies on the unit of transcription of C8 DNA puff. Braz arch biol technol. 1989; 32(1).
Cloning of sequences from B2 DNA puff of Rhynchosciara salivary gland chromosomes. Pirrota V, Bonaldo MF, Amabis JM, Santelli RV, Frydman HM, Cattapan LDN, Lara FJS. 1988; 31(1).

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 23 publications over 16 distinct years, with a maximum of 4 publications in 2018

Bar chart showing 23 publications over 16 distinct years, with a maximum of 4 publications in 2018

Year	Publications
1988	1
1989	1
1993	1
2001	1
2005	1
2006	3
2007	1
2011	1
2013	1
2014	1
2017	2
2018	4
2019	2
2020	1
2021	1
2023	1

Contact for Mentoring:

617.358.5070

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