Keywords
Last Name

Pinghua Liu, PhD

TitleAssociate Professor
InstitutionBoston University College of Arts and Sciences
DepartmentChemistry
Address590 Commonwealth Avenue
Boston MA 02215
Phone(617) 353-2481
Fax(617) 353-6466
 Research Expertise & Professional Interests
The Liu Group’s research is at the interface of Chemistry and Biology. Their work focuses on a) mechanistic studies and the application of pharmacologically important natural products using organic, molecular biology as well as biophysical methods, and b) the chemical basis of biological clock with the ultimate goal of elucidating the time-recording mechanism in the ageing process. Their work’s main focuses are the chemical basis of pathogen and host interactions, and the chemical nature of the biological clock. His projects address two major areas (1) mechanistic studies of two metallo-proteins (IspG and IspH) in the corresponding process, and (2) identification of other components in these two processes.

Chemical Basis of Biological Clock – The lab’s research into circadian rhythms (Should we explain what they are, or will most people know) the area is divided into three sub-areas: (1) Determining the chemical basis and detailed signal transduction pathways in circadian clock, (2) mechanistic studies on enzymes involved in histone-posttranslational modifications, and (3) Identification of the inhibitors for the clock-related enzymes for therapeutic purposes.

Isoprenoid Biosynthesis – The lab’s research on isoprenoids, one of the largest and most structurally diverse groups of metabolites found in nature, is divided into four sub-areas: (1) Mechanistic studies of the IspG and IspH, (2) Development of mechanism-based inhibitors of IspG, IspH as antibiotics and antimalaria drugs, (3) Building an isoprenoid library, biological activity evaluation, and isoprenoid production using bioengineering, and (4) Mechanistic studies of the compound 8 triggered human innate-immune response signal transduction pathway.

Techniques & Resources:

Enzymology – This includes organic synthesis, synthesis of isotopically labeled probes, sub-cloning, protein expression and purification, steady-state and pre-steady state kinetics, inhibitor design and evaluation, and mechanistic studies.

Genetics – The Liu lab uses transcriptional, post-transcriptional, and post-translational regulation, gene knockout, gene replacement, and metabolic engineering.

Spectroscopies – We utilize EPR and Mössbauer for metallo-enzyme characterizations.

 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JF, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Héroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A. Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography. Structure. 2016 Apr 05; 24(4):631-40. PMID: 26996959.
    View in: PubMed
  2. Yan W, Song H, Song F, Guo Y, Wu CH, Sae Her A, Pu Y, Wang S, Naowarojna N, Weitz A, Hendrich MP, Costello CE, Zhang L, Liu P, Zhang YJ. Endoperoxide formation by an a-ketoglutarate-dependent mononuclear non-haem iron enzyme. Nature. 2015 Nov 26; 527(7579):539-43. PMID: 26524521.
    View in: PubMed
  3. Song H, Hu W, Naowarojna N, Her AS, Wang S, Desai R, Qin L, Chen X, Liu P. Mechanistic studies of a novel C-S lyase in ergothioneine biosynthesis: the involvement of a sulfenic acid intermediate. Sci Rep. 2015 Jul 07; 5:11870. PMID: 26149121.
    View in: PubMed
  4. Ning L, Li Z, Wang G, Hu W, Hou Q, Tong Y, Zhang M, Chen Y, Qin L, Chen X, Man HY, Liu P, He J. Quantitative assessment of single-cell whole genome amplification methods for detecting copy number variation using hippocampal neurons. Sci Rep. 2015 Jun 19; 5:11415. PMID: 26091148.
    View in: PubMed
  5. Song H, Her AS, Raso F, Zhen Z, Huo Y, Liu P. Cysteine oxidation reactions catalyzed by a mononuclear non-heme iron enzyme (OvoA) in ovothiol biosynthesis. Org Lett. 2014 Apr 18; 16(8):2122-5. PMID: 24684381.
    View in: PubMed
  6. Song H, Leninger M, Lee N, Liu P. Regioselectivity of the oxidative C-S bond formation in ergothioneine and ovothiol biosyntheses. Org Lett. 2013 Sep 20; 15(18):4854-7. PMID: 24016264.
    View in: PubMed
  7. Daughtry KD, Xiao Y, Stoner-Ma D, Cho E, Orville AM, Liu P, Allen KN. Quaternary ammonium oxidative demethylation: X-ray crystallographic, resonance Raman, and UV-visible spectroscopic analysis of a Rieske-type demethylase. J Am Chem Soc. 2012 Feb 8; 134(5):2823-34. PMID: 22224443.
    View in: PubMed
  8. Xiao Y, Chang WC, Liu HW, Liu P. Study of IspH, a key enzyme in the methylerythritol phosphate pathway using fluoro-substituted substrate analogues. Org Lett. 2011 Nov 04; 13(21):5912-5. PMID: 21981393.
    View in: PubMed
  9. Xiao Y, Rooker D, You Q, Meyers CL, Liu P. IspG-catalyzed positional isotopic exchange in methylerythritol cyclodiphosphate of the deoxyxylulose phosphate pathway: mechanistic implications. Chembiochem. 2011 Mar 07; 12(4):527-30. PMID: 22238143.
    View in: PubMed
  10. Xiao Y, Nyland RL, Meyers CL, Liu P. Methylerythritol cyclodiphosphate (MEcPP) in deoxyxylulose phosphate pathway: synthesis from an epoxide and mechanisms. Chem Commun (Camb). 2010 Oct 14; 46(38):7220-2. PMID: 20820543.
    View in: PubMed
  11. Xiao Y, Zahariou G, Sanakis Y, Liu P. IspG enzyme activity in the deoxyxylulose phosphate pathway: roles of the iron-sulfur cluster. Biochemistry. 2009 Nov 10; 48(44):10483-5. PMID: 19821611.
    View in: PubMed
  12. Xiao Y, Chu L, Sanakis Y, Liu P. Revisiting the IspH catalytic system in the deoxyxylulose phosphate pathway: achieving high activity. J Am Chem Soc. 2009 Jul 29; 131(29):9931-3. PMID: 19583210.
    View in: PubMed
  13. Xiao Y, Machacek M, Lee K, Kuzuyama T, Liu P. Prenyltransferase substrate binding pocket flexibility and its application in isoprenoid profiling. Mol Biosyst. 2009 Sep; 5(9):913-7. PMID: 19668852.
    View in: PubMed
  14. Xiao Y, Lee K, Liu P. Syntheses of the P-methylase substrates of the bialaphos biosynthetic pathway. Org Lett. 2008 Dec 18; 10(24):5521-4. PMID: 19053721.
    View in: PubMed
  15. Xiao Y, Zhao ZK, Liu P. Mechanistic studies of IspH in the deoxyxylulose phosphate pathway: heterolytic C-O bond cleavage at C4 position. J Am Chem Soc. 2008 Feb 20; 130(7):2164-5. PMID: 18217765.
    View in: PubMed
  16. Xiao Y, Liu P. IspH protein of the deoxyxylulose phosphate pathway: mechanistic studies with C1-deuterium-labeled substrate and fluorinated analogue. Angew Chem Int Ed Engl. 2008; 47(50):9722-5. PMID: 18980164.
    View in: PubMed
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