G. Graham Shipley, PhD
Professor
Boston University School of Medicine
Dept of Physiology & Biophysics

PhD, University of Nottingham



Membrane and Receptor Biology:

Our interests center around the structure and function of cell membranes, receptor-ligand interactions and transmembrane signaling mechanisms. A combination of biochemical, chemical and biophysical approaches are used to probe: (1) membrane lipid conformation, structure, properties and interactions, (2) membrane receptor-lipid interactions and (3) membrane receptor-ligand interactions. Current approaches involve the isolation of membrane-associated proteins and lipids (e.g. low density lipoprotein (LDL) receptor, insulin receptor, integrins, gangliosides), their reconstitution with well characterized lipid monolayer and bilayer matrices and structural studies of the protein-lipid assemblies. Ligand binding studes include LDL receptor/LDL, insulin receptor/insulin, and ganglioside/toxin interactions. The biochemical methods involved include lipid and protein isolation, gel/affinity chromatography, antibody techniques, immunoblotting, detergent solubilization, lipid-protein reconstitution, etc. Chemical methods include lipid sythesis, TLC, HPLC, IR and NMR. The biophysical approach involves x-ray diffraction, protein crystallography, electron microscopy/image reconstruction, surface chemistry and calorimetric and spectroscopic (CD, NMR) methods.

Graduate Faculty (Primary Mentor of Grad Students)
Boston University School of Medicine, Division of Graduate Medical Sciences


Professor
Boston University School of Medicine
Department of Biochemistry



2014 Boston University School of Medicine: Educator of the Year in Graduate Medical Sciences, Doctoral Degree Programs


Glycosphingolipids and GM1-Cholera Toxin Interactions
01/01/1997 - 12/31/2002 (PI)
NIH/National Heart, Lung, and Blood Institute
5 R01 HL57405 04



Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

  1. Doyle DA, Shipley GG. Membranes: editorial overview. Curr Opin Struct Biol. 2009 Aug; 19(4):369-71. PMID: 19709877; DOI: 10.1016/j.sbi.2009.07.011;.
  2. Jeon H, Shipley GG. Localization of the N-terminal domain of the low density lipoprotein receptor. J Biol Chem. 2000 Sep 29; 275(39):30465-70. PMID: 10889195.
  3. Jeon H, Shipley GG. Vesicle-reconstituted low density lipoprotein receptor. Visualization by cryoelectron microscopy. J Biol Chem. 2000 Sep 29; 275(39):30458-64. PMID: 10889196.
  4. Shipley GG. Bilayers and nonbilayers: structure, forces and protein crystallization. Curr Opin Struct Biol. 2000 Aug; 10(4):471-3. PMID: 10981637.
  5. Saxena K, Duclos RI, Sripada PK, Shipley GG. Unusual hydration properties of C16:0 sulfatide bilayer membranes. Biophys J. 2000 Jul; 79(1):385-93. PMID: 10866964; PMCID: PMC1300942.
  6. Saxena K, Zimmermann P, Schmidt RR, Shipley GG. Bilayer properties of totally synthetic C16:0-lactosyl-ceramide. Biophys J. 2000 Jan; 78(1):306-12. PMID: 10620294; PMCID: PMC1300638.
  7. Woldin CN, Hing FS, Lee J, Pilch PF, Shipley GG. Structural studies of the detergent-solubilized and vesicle-reconstituted insulin receptor. J Biol Chem. 1999 Dec 3; 274(49):34981-92.View Related Profiles. PMID: 10574975.
  8. Saxena K, Duclos RI, Zimmermann P, Schmidt RR, Shipley GG. Structure and properties of totally synthetic galacto- and gluco-cerebrosides. J Lipid Res. 1999 May; 40(5):839-49. PMID: 10224153.
  9. Saxena K, Shipley GG. Structural studies of detergent-solubilized and vesicle-reconstituted low-density lipoprotein (LDL) receptor. Biochemistry. 1997 Dec 16; 36(50):15940-8. PMID: 9398328.
  10. Maulik PR, Shipley GG. N-palmitoyl sphingomyelin bilayers: structure and interactions with cholesterol and dipalmitoylphosphatidylcholine. Biochemistry. 1996 Jun 18; 35(24):8025-34. PMID: 8672507.
Showing 10 of 94 results. Show More

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 59 publications over 20 distinct years, with a maximum of 9 publications in 1987

YearPublications
19802
19812
19821
19834
19844
19851
19863
19879
19881
19893
19905
19912
19921
19942
19957
19963
19971
19992
20005
20091
Contact for Mentoring:


700 Albany St Ctr for Adv Biomed Res
Boston MA 02118
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