Keywords
Last Name

G. Graham Shipley, PhD

TitleProfessor
InstitutionBoston University School of Medicine
DepartmentPhysiology & Biophysics
Address700 Albany St - CABR
Boston MA 02118
Phone(617) 638-4009
ORCID ORCID Icon0000-0001-9603-5555
Other Positions
TitleGraduate Faculty (Primary Mentor of Grad Students)
InstitutionBoston University School of Medicine, Graduate Medical Sciences

TitleProfessor
InstitutionBoston University School of Medicine
DepartmentBiochemistry

TitleResearch Professor
InstitutionBoston University School of Medicine
DepartmentMedicine

 Awards and Honors

Start-EndDescription
2014Boston University School of Medicine: Educator of the Year in Graduate Medical Sciences, Doctoral Degree Programs
 Research Expertise & Professional Interests
Membrane and Receptor Biology:

Our interests center around the structure and function of cell membranes, receptor-ligand interactions and transmembrane signaling mechanisms. A combination of biochemical, chemical and biophysical approaches are used to probe: (1) membrane lipid conformation, structure, properties and interactions, (2) membrane receptor-lipid interactions and (3) membrane receptor-ligand interactions. Current approaches involve the isolation of membrane-associated proteins and lipids (e.g. low density lipoprotein (LDL) receptor, insulin receptor, integrins, gangliosides), their reconstitution with well characterized lipid monolayer and bilayer matrices and structural studies of the protein-lipid assemblies. Ligand binding studes include LDL receptor/LDL, insulin receptor/insulin, and ganglioside/toxin interactions. The biochemical methods involved include lipid and protein isolation, gel/affinity chromatography, antibody techniques, immunoblotting, detergent solubilization, lipid-protein reconstitution, etc. Chemical methods include lipid sythesis, TLC, HPLC, IR and NMR. The biophysical approach involves x-ray diffraction, protein crystallography, electron microscopy/image reconstruction, surface chemistry and calorimetric and spectroscopic (CD, NMR) methods.

 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Doyle DA, Shipley GG. Membranes: editorial overview. Curr Opin Struct Biol. 2009 Aug; 19(4):369-71.
    View in: PubMed
  2. Jeon H, Shipley GG. Localization of the N-terminal domain of the low density lipoprotein receptor. J Biol Chem. 2000 Sep 29; 275(39):30465-70.
    View in: PubMed
  3. Jeon H, Shipley GG. Vesicle-reconstituted low density lipoprotein receptor. Visualization by cryoelectron microscopy. J Biol Chem. 2000 Sep 29; 275(39):30458-64.
    View in: PubMed
  4. Shipley GG. Bilayers and nonbilayers: structure, forces and protein crystallization. Curr Opin Struct Biol. 2000 Aug; 10(4):471-3.
    View in: PubMed
  5. Saxena K, Duclos RI, Sripada PK, Shipley GG. Unusual hydration properties of C16:0 sulfatide bilayer membranes. Biophys J. 2000 Jul; 79(1):385-93.
    View in: PubMed
  6. Saxena K, Zimmermann P, Schmidt RR, Shipley GG. Bilayer properties of totally synthetic C16:0-lactosyl-ceramide. Biophys J. 2000 Jan; 78(1):306-12.
    View in: PubMed
  7. Woldin CN, Hing FS, Lee J, Pilch PF, Shipley GG. Structural studies of the detergent-solubilized and vesicle-reconstituted insulin receptor. J Biol Chem. 1999 Dec 3; 274(49):34981-92.
    View in: PubMed
  8. Saxena K, Duclos RI, Zimmermann P, Schmidt RR, Shipley GG. Structure and properties of totally synthetic galacto- and gluco-cerebrosides. J Lipid Res. 1999 May; 40(5):839-49.
    View in: PubMed
  9. Saxena K, Shipley GG. Structural studies of detergent-solubilized and vesicle-reconstituted low-density lipoprotein (LDL) receptor. Biochemistry. 1997 Dec 16; 36(50):15940-8.
    View in: PubMed
  10. Maulik PR, Shipley GG. N-palmitoyl sphingomyelin bilayers: structure and interactions with cholesterol and dipalmitoylphosphatidylcholine. Biochemistry. 1996 Jun 18; 35(24):8025-34.
    View in: PubMed
  11. Maulik PR, Shipley GG. Interactions of N-stearoyl sphingomyelin with cholesterol and dipalmitoylphosphatidylcholine in bilayer membranes. Biophys J. 1996 May; 70(5):2256-65.
    View in: PubMed
  12. Reed RA, Shipley GG. Properties of ganglioside GM1 in phosphatidylcholine bilayer membranes. Biophys J. 1996 Mar; 70(3):1363-72.
    View in: PubMed
  13. Haas NS, Shipley GG. Structure and properties of N-palmitoleoylgalactosylsphingosine (cerebroside). Biochim Biophys Acta. 1995 Dec 13; 1240(2):133-41.
    View in: PubMed
  14. Maulik PR, Shipley GG. X-ray diffraction and calorimetric study of N-lignoceryl sphingomyelin membranes. Biophys J. 1995 Nov; 69(5):1909-16.
    View in: PubMed
  15. Hing FS, Shipley GG. Molecular interactions of ether-linked phospholipids. Biochemistry. 1995 Sep 19; 34(37):11904-9.
    View in: PubMed
  16. Shah J, Atienza JM, Duclos RI, Rawlings AV, Dong Z, Shipley GG. Structural and thermotropic properties of synthetic C16:0 (palmitoyl) ceramide: effect of hydration. J Lipid Res. 1995 Sep; 36(9):1936-44.
    View in: PubMed
  17. Shah J, Atienza JM, Rawlings AV, Shipley GG. Physical properties of ceramides: effect of fatty acid hydroxylation. J Lipid Res. 1995 Sep; 36(9):1945-55.
    View in: PubMed
  18. Zhang RG, Westbrook ML, Westbrook EM, Scott DL, Otwinowski Z, Maulik PR, Reed RA, Shipley GG. The 2.4 A crystal structure of cholera toxin B subunit pentamer: choleragenoid. J Mol Biol. 1995 Aug 25; 251(4):550-62.
    View in: PubMed
  19. Zhang RG, Scott DL, Westbrook ML, Nance S, Spangler BD, Shipley GG, Westbrook EM. The three-dimensional crystal structure of cholera toxin. J Mol Biol. 1995 Aug 25; 251(4):563-73.
    View in: PubMed
  20. Shah J, Duclos RI, Shipley GG. Structure and thermotropic properties of 1-stearoyl-2-acetyl-phosphatidylcholine bilayer membranes. Biophys J. 1994 May; 66(5):1469-78.
    View in: PubMed
  21. Cabral-Lilly D, Sosinsky GE, Reed RA, McDermott MR, Shipley GG. Orientation of cholera toxin bound to model membranes. Biophys J. 1994 Apr; 66(4):935-41.
    View in: PubMed
  22. Shah J, Shipley GG. Circular dichroic studies of protein kinase C and its interactions with calcium and lipid vesicles. Biochim Biophys Acta. 1992 Feb 13; 1119(1):19-26.
    View in: PubMed
  23. Hing FS, Maulik PR, Shipley GG. Structure and interactions of ether- and ester-linked phosphatidylethanolamines. Biochemistry. 1991 Sep 17; 30(37):9007-15.
    View in: PubMed
  24. Maulik PR, Sripada PK, Shipley GG. Structure and thermotropic properties of hydrated N-stearoyl sphingomyelin bilayer membranes. Biochim Biophys Acta. 1991 Feb 25; 1062(2):211-9.
    View in: PubMed
  25. Maulik PR, Ruocco MJ, Shipley GG. Hydrocarbon chain packing modes in lipids: effect of altered sub-cell dimensions and chain rotation. Chem Phys Lipids. 1990 Dec; 56(2-3):123-33.
    View in: PubMed
  26. Shipley GG. Structural studies of the lipid components of bile. Hepatology. 1990 Sep; 12(3 Pt 2):33S-37S; discussion 37S-38S.
    View in: PubMed
  27. Shah J, Sripada PK, Shipley GG. Structure and properties of mixed-chain phosphatidylcholine bilayers. Biochemistry. 1990 May 1; 29(17):4254-62.
    View in: PubMed
  28. Maulik PR, Reed RA, Sparrow DA, Sparrow JT, Shipley GG. Crystallization and preliminary x-ray diffraction study of synthetic apolipoprotein E fragment (residues 129-169). J Biol Chem. 1990 Jan 5; 265(1):490-2.
    View in: PubMed
  29. Haas NS, Sripada PK, Shipley GG. Effect of chain-linkage on the structure of phosphatidyl choline bilayers. Hydration studies of 1-hexadecyl 2-palmitoyl-sn-glycero-3-phosphocholine. Biophys J. 1990 Jan; 57(1):117-24.
    View in: PubMed
  30. Lee CW, Das Gupta SK, Mattai J, Shipley GG, Abdel-Mageed OH, Makriyannis A, Griffin RG. Characterization of the L lambda phase in trehalose-stabilized dry membranes by solid-state NMR and X-ray diffraction. Biochemistry. 1989 Jun 13; 28(12):5000-9.
    View in: PubMed
  31. Mattai J, Hauser H, Demel RA, Shipley GG. Interactions of metal ions with phosphatidylserine bilayer membranes: effect of hydrocarbon chain unsaturation. Biochemistry. 1989 Mar 7; 28(5):2322-30.
    View in: PubMed
  32. Reed RA, Shipley GG. Effect of chain unsaturation on the structure and thermotropic properties of galactocerebrosides. Biophys J. 1989 Feb; 55(2):281-92.
    View in: PubMed
  33. Maulik PR, Reed RA, Shipley GG. Crystallization and preliminary x-ray diffraction study of cholera toxin B-subunit. J Biol Chem. 1988 Jul 5; 263(19):9499-501.
    View in: PubMed
  34. Kim JT, Mattai J, Shipley GG. Bilayer interactions of ether- and ester-linked phospholipids: dihexadecyl- and dipalmitoylphosphatidylcholines. Biochemistry. 1987 Oct 20; 26(21):6599-603.
    View in: PubMed
  35. Kim JT, Mattai J, Shipley GG. Gel phase polymorphism in ether-linked dihexadecylphosphatidylcholine bilayers. Biochemistry. 1987 Oct 20; 26(21):6592-8.
    View in: PubMed
  36. Mattai J, Sripada PK, Shipley GG. Mixed-chain phosphatidylcholine bilayers: structure and properties. Biochemistry. 1987 Jun 16; 26(12):3287-97.
    View in: PubMed
  37. Sripada PK, Maulik PR, Hamilton JA, Shipley GG. Partial synthesis and properties of a series of N-acyl sphingomyelins. J Lipid Res. 1987 Jun; 28(6):710-8.
    View in: PubMed
  38. Goldfine H, Johnston NC, Mattai J, Shipley GG. Regulation of bilayer stability in Clostridium butyricum: studies on the polymorphic phase behavior of the ether lipids. Biochemistry. 1987 May 19; 26(10):2814-22.
    View in: PubMed
  39. Speyer JB, Sripada PK, Das Gupta SK, Shipley GG, Griffin RG. Magnetic orientation of sphingomyelin-lecithin bilayers. Biophys J. 1987 Apr; 51(4):687-91.
    View in: PubMed
  40. Reed RA, Mattai J, Shipley GG. Interaction of cholera toxin with ganglioside GM1 receptors in supported lipid monolayers. Biochemistry. 1987 Feb 10; 26(3):824-32.
    View in: PubMed
  41. Mattai J, Witzke NM, Bittman R, Shipley GG. Structure and thermotropic properties of hydrated 1-eicosyl-2-dodecyl-rac-glycero-3-phosphocholine and 1-dodecyl-2-eicosyl-rac-glycero-3-phosphocholine bilayer membranes. Biochemistry. 1987 Jan 27; 26(2):623-33.
    View in: PubMed
  42. Reed RA, Shipley GG. Structure and metastability of N-lignocerylgalactosylsphingosine (cerebroside) bilayers. Biochim Biophys Acta. 1987 Jan 26; 896(2):153-64.
    View in: PubMed
  43. Maulik PR, Atkinson D, Shipley GG. X-ray scattering of vesicles of N-acyl sphingomyelins. Determination of bilayer thickness. Biophys J. 1986 Dec; 50(6):1071-7.
    View in: PubMed
  44. Ruocco MJ, Shipley GG. Thermal and structural behavior of natural cerebroside 3-sulfate in bilayer membranes. Biochim Biophys Acta. 1986 Jul 24; 859(2):246-56.
    View in: PubMed
  45. Mattai J, Shipley GG. The kinetics of formation and structure of the low-temperature phase of 1-stearoyl-lysophosphatidylcholine. Biochim Biophys Acta. 1986 Jul 24; 859(2):257-65.
    View in: PubMed
  46. Hauser H, Shipley GG. Comparative structural aspects of cation binding to phosphatidylserine bilayers. Biochim Biophys Acta. 1985 Mar 14; 813(2):343-6.
    View in: PubMed
  47. Ruocco MJ, Shipley GG. Interaction of cholesterol with galactocerebroside and galactocerebroside-phosphatidylcholine bilayer membranes. Biophys J. 1984 Dec; 46(6):695-707.
    View in: PubMed
  48. Mulukutla S, Shipley GG. Structure and thermotropic properties of phosphatidylethanolamine and its N-methyl derivatives. Biochemistry. 1984 May 22; 23(11):2514-9.
    View in: PubMed
  49. Hauser H, Shipley GG. Interactions of divalent cations with phosphatidylserine bilayer membranes. Biochemistry. 1984 Jan 3; 23(1):34-41.
    View in: PubMed
  50. Atkinson D, Shipley GG. Structural studies of plasma lipoproteins. Basic Life Sci. 1984; 27:211-26.
    View in: PubMed
  51. Ruocco MJ, Shipley GG. Hydration of N-palmitoylgalactosylsphingosine compared to monosaccharide hydration. Biochim Biophys Acta. 1983 Nov 9; 735(2):305-8.
    View in: PubMed
  52. Serrallach EN, Dijkman R, de Haas GH, Shipley GG. Structure and thermotropic properties of 1,3-dipalmitoyl-glycero-2-phosphocholine. J Mol Biol. 1983 Oct 15; 170(1):155-74.
    View in: PubMed
  53. Ruocco MJ, Shipley GG, Oldfield E. Galactocerebroside-phospholipid interactions in bilayer membranes. Biophys J. 1983 Jul; 43(1):91-101.
    View in: PubMed
  54. Hauser H, Shipley GG. Interactions of monovalent cations with phosphatidylserine bilayer membranes. Biochemistry. 1983 Apr 26; 22(9):2171-8.
    View in: PubMed
  55. Hauser H, Paltauf F, Shipley GG. Structure and thermotropic behavior of phosphatidylserine bilayer membranes. Biochemistry. 1982 Mar 2; 21(5):1061-7.
    View in: PubMed
  56. Hauser H, Shipley GG. Crystallization of phosphatidylserine bilayers induced by lithium. J Biol Chem. 1981 Nov 25; 256(22):11377-80.
    View in: PubMed
  57. Ruocco MJ, Atkinson D, Small DM, Skarjune RP, Oldfield E, Shipley GG. X-ray diffraction and calorimetric study of anhydrous and hydrated N-palmitoylgalactosylsphingosine (cerebroside). Biochemistry. 1981 Oct 13; 20(21):5957-66.
    View in: PubMed
  58. Estep TN, Calhoun WI, Barenholz Y, Biltonen RL, Shipley GG, Thompson TE. Evidence for metastability in stearoylsphingomyelin bilayers. Biochemistry. 1980 Jan 8; 19(1):20-4.
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  59. Calhoun WI, Shipley GG. Fatty acid composition and thermal behavior of natural sphingomyelins. Biochim Biophys Acta. 1979 Aug 23; 555(3):436-41.
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  60. Janiak MJ, Small DM, Shipley GG. Temperature and compositional dependence of the structure of hydrated dimyristoyl lecithin. J Biol Chem. 1979 Jul 10; 254(13):6068-78.
    View in: PubMed
  61. Loomis CR, Shipley GG, Small DM. The phase behavior of hydrated cholesterol. J Lipid Res. 1979 May; 20(4):525-35.
    View in: PubMed
  62. Calhoun WI, Shipley GG. Sphingomyelin--lecithin bilayers and their interaction with cholesterol. Biochemistry. 1979 May 1; 18(9):1717-22.
    View in: PubMed
  63. Janiak MJ, Small DM, Shipley GG. Interactions of cholesterol esters with phospholipids: cholesteryl myristate and dimyristoyl lecithin. J Lipid Res. 1979 Feb; 20(2):183-99.
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  64. Curatolo W, Verma SP, Sakura JD, Small DM, Shipley GG, Wallach DF. Structural effects of myelin proteolipid apoprotein on phospholipids: a Raman spectroscopic study. Biochemistry. 1978 May 2; 17(9):1802-7.
    View in: PubMed
  65. Tall AR, Small DM, Deckelbaum RJ, Shipley GG. Structure and thermodynamic properties of high density lipoprotein recombinants. J Biol Chem. 1977 Jul 10; 252(13):4701-11.
    View in: PubMed
  66. Untrach SH, Shipley GG. Molecular interactions between lecithin and sphingomyelin. Temperature- and composition-dependent phase separation. J Biol Chem. 1977 Jul 10; 252(13):4449-57.
    View in: PubMed
  67. Curatolo W, Small DM, Shipley GG. Phase behavior and structural characteristics of hydrated bovine brain gangliosides. Biochim Biophys Acta. 1977 Jul 4; 468(1):11-20.
    View in: PubMed
  68. Curatolo W, Sakura JD, Small DM, Shipley GG. Protein-lipid interactions: recombinants of the proteolipid apoprotein of myelin with dimyristoyllecithin. Biochemistry. 1977 May 31; 16(11):2313-9.
    View in: PubMed
  69. Tall AR, Deckelbaum RJ, Small DM, Shipley GG. Thermal behavior of human plasma high density lipoprotein. Biochim Biophys Acta. 1977 Apr 26; 487(1):145-33.
    View in: PubMed
  70. Deckelbaum RJ, Shipley GG, Small DM. Structure and interactions of lipids in human plasma low density lipoproteins. J Biol Chem. 1977 Jan 25; 252(2):744-54.
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  71. Janiak MJ, Small DM, Shipley GG. Nature of the Thermal pretransition of synthetic phospholipids: dimyristolyl- and dipalmitoyllecithin. Biochemistry. 1976 Oct 19; 15(21):4575-80.
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  72. Sears B, Deckelbaum RJ, Janiak MJ, Shipley GG, Small DM. Temperature-dependent 13C nuclear magnetic resonance studies of human serum low density lipoproteins. Biochemistry. 1976 Sep 21; 15(19):4151-7.
    View in: PubMed
  73. Katz SS, Shipley GG, Small DM. Physical chemistry of the lipids of human atherosclerotic lesions. Demonstration of a lesion intermediate between fatty streaks and advanced plaques. J Clin Invest. 1976 Jul; 58(1):200-11.
    View in: PubMed
  74. Tall AR, Shipley GG, Small DM. Conformational and thermodynamic properties of apo A-1 of human plasma high density lipoproteins. J Biol Chem. 1976 Jun 25; 251(12):3749-55.
    View in: PubMed
  75. North BE, Shipley GG, Small DM. The thermal transitions and structural properties of 5alpha-cholestan-3beta-ol esters of aliphatic acids. Biochim Biophys Acta. 1976 Mar 26; 424(3):376-85.
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  76. Tall AR, Small DM, Shipley GG, Lees RS. Apoprotein stability and lipid-protein interactions in human plasma high density lipoproteins. Proc Natl Acad Sci U S A. 1975 Dec; 72(12):4940-2.
    View in: PubMed
  77. Deckelbaum RJ, Shipley GG, Small DM, Lees RS, George PK. Thermal transitions in human plasma low density lipoproteins. Science. 1975 Oct 24; 190(4212):392-4.
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  78. Hitchcock PB, Mason R, Shipley GG. Phospholipid arrangements in multilayers and artificial membranes: quantitative analysis of the X-ray diffraction data from a multilayer of 1,2-dimyristoyl-DL-phosphatidylethanolamine. J Mol Biol. 1975 May 15; 94(2):297-9.
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  79. Hitchcock PB, Mason R, Thomas KM, Shipley GG. Structural chemistry of 1,2 dilauroyl-DL-phosphatidylethanolamine: molecular conformation and intermolecular packing of phospholipids. Proc Natl Acad Sci U S A. 1974 Aug; 71(8):3036-40.
    View in: PubMed
  80. Small DM, Shipley GG. Physical-chemical basis of lipid deposition in atherosclerosis. Science. 1974 Jul 19; 185(4147):222-9.
    View in: PubMed
  81. Loomis CR, Janiak MJ, Small DM, Shipley GG. The binary phase diagram of lecithin and cholesteryl linolenate. J Mol Biol. 1974 Jun 25; 86(2):309-24.
    View in: PubMed
  82. Janiak MJ, Loomis CR, Shipley GG, Small DM. The ternary phase diagram of lecithin, cholesteryl linolenate and water: phase behavior and structure. J Mol Biol. 1974 Jun 25; 86(2):325-39.
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  83. Shipley GG, Avecilla LS, Small DM. Phase behavior and structure of aqueous dispersions of sphingomyelin. J Lipid Res. 1974 Mar; 15(2):124-31.
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  84. Atkinson D, Hauser H, Shipley GG, Stubbs JM. Structure and morphology of phosphatidylserine dispersions. Biochim Biophys Acta. 1974 Feb 26; 339(1):10-29.
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  85. Green JP, Phillips MC, Shipley GG. Structural investigations of lipid, polypeptide and protein multilayers. Biochim Biophys Acta. 1973 Dec 22; 330(3):243-53.
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  86. Shipley GG, Green JP, Nichols BW. The phase behavior of monogalactosyl, digalactosyl, and sulphoquinovosyl diglycerides. Biochim Biophys Acta. 1973 Jul 18; 311(4):531-44.
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  87. Cullen J, Phillips MC, Shipley GG. The effects of temperature on the composition and physical properties of the lipids of Pseudomonas fluorescens. Biochem J. 1971 Dec; 125(3):733-42.
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  88. Shipley GG, Leslie RB, Chapman D. X-ray diffraction study of the interaction of phospholipids with cytochrome c in the aqueous phase. Nature. 1969 May 10; 222(5193):561-2.
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  89. Shipley GG, Leslie RB, Chapman D. Small-angle x-ray scattering studies of cytochrome c-phospholipid complexes. Biochim Biophys Acta. 1969 Jan 28; 173(1):1-10.
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  90. Chapman D, Fluck DJ, Penkett SA, Shipley GG. Physical studies of phospholipids. X. The effect of sonication of aqueous dispersions of egg yolk lecithin. Biochim Biophys Acta. 1968 Sep 17; 163(2):255-61.
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