Keywords
Last Name

Paul Pilch, PhD

TitleProfessor
InstitutionBoston University School of Medicine
DepartmentBiochemistry
Address72 E. Concord St
Boston MA 02118
Phone(617) 638-4044
ORCID ORCID Icon0000-0003-1997-0499
Other Positions
TitleGraduate Faculty (Primary Mentor of Grad Students)
InstitutionBoston University School of Medicine, Graduate Medical Sciences

TitleProfessor
InstitutionBoston University School of Medicine
DepartmentMedicine
DivisionMolecular Medicine

TitleResearch Professor
InstitutionBoston University School of Medicine
DepartmentPhysiology & Biophysics

InstitutionBoston Medical Center

 Research Expertise & Professional Interests
Research Interests: Cell biology of fuel utilization in adipocytes and skeletal muscle.

The modern Western diet coupled with a sedentary lifestyle has led to an epidemic of obesity, a consequence of which is a dramatic rise in the incidence of type II diabetes mellitus, a malfunction in insulin-regulated metabolism. At the cellular level, type II diabetes is characterized by failure of insulin to act in liver, muscle and fat. We study aspects of insulin signaling and action in the latter two tissues. Insulin resistance in muscle (and fat) derives from the failure of insulin to activate the tissue-specific glucose transporter GLUT4. The activation mechanism for this process involves vesicle trafficking and protein targeting with regard to GLUT4 and the insulin receptor. We are characterizing the formation and protein content of GLUT4-containing vesicles; we are trying to identify the organelles through which they pass on their way to and from the cell surface and we are determining the communication mechanism(s) (signaling) from the insulin receptor to the GLUT4-containing vesicles. These studies involve both fat and muscle cells, and we are also studying the physiological role of cell surface (plasma membrane) micro-domains called caveolae that are particularly abundant in these tissues. We have evidence for the hypothesis that caveolae (for little caves that are small invaginations of the plasma membrane into the cytosol) are involved in lipid trafficking.

We continue to study other aspects of adipocyte and muscle cell biology to understand the interplay between glucose and fat metabolism as well as the interplay between adipocytes and muscle required for overall metabolic homeostasis. Indeed, we wish to uncover the mechanism(s) by exercise also regulates some of these same parameters independent of insulin. Understanding these pathways will help us to figure out how they are compromised in pathophysiological states such as diabetes.

 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Ding SY, Lee MJ, Summer R, Liu L, Fried SK, Pilch PF. Pleiotropic effects of cavin-1 deficiency on lipid metabolism. J Biol Chem. 2014 Mar 21; 289(12):8473-83.
    View in: PubMed
  2. Fernández-Rojo MA, Gongora M, Fitzsimmons RL, Martel N, Martin SD, Nixon SJ, Brooks AJ, Ikonomopoulou MP, Martin S, Lo HP, Myers SA, Restall C, Ferguson C, Pilch PF, McGee SL, Anderson RL, Waters MJ, Hancock JF, Grimmond SM, Muscat GE, Parton RG. Caveolin-1 is necessary for hepatic oxidative lipid metabolism: evidence for crosstalk between caveolin-1 and bile acid signaling. Cell Rep. 2013 Jul 25; 4(2):238-47.
    View in: PubMed
  3. Govender P, Romero F, Shah D, Paez J, Ding SY, Liu L, Gower A, Baez E, Aly SS, Pilch P, Summer R. Cavin1; a regulator of lung function and macrophage phenotype. PLoS One. 2013; 8(4):e62045.
    View in: PubMed
  4. Scotti E, Calamai M, Goulbourne CN, Zhang L, Hong C, Lin RR, Choi J, Pilch PF, Fong LG, Zou P, Ting AY, Pavone FS, Young SG, Tontonoz P. IDOL stimulates clathrin-independent endocytosis and multivesicular body-mediated lysosomal degradation of the low-density lipoprotein receptor. Mol Cell Biol. 2013 Apr; 33(8):1503-14.
    View in: PubMed
  5. Hill MM, Daud NH, Aung CS, Loo D, Martin S, Murphy S, Black DM, Barry R, Simpson F, Liu L, Pilch PF, Hancock JF, Parat MO, Parton RG. Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1. PLoS One. 2012; 7(8):e43041.
    View in: PubMed
  6. Breen MR, Camps M, Carvalho-Simoes F, Zorzano A, Pilch PF. Cholesterol depletion in adipocytes causes caveolae collapse concomitant with proteosomal degradation of cavin-2 in a switch-like fashion. PLoS One. 2012; 7(4):e34516.
    View in: PubMed
  7. Tkachenko E, Tse D, Sideleva O, Deharvengt SJ, Luciano MR, Xu Y, McGarry CL, Chidlow J, Pilch PF, Sessa WC, Toomre DK, Stan RV. Caveolae, fenestrae and transendothelial channels retain PV1 on the surface of endothelial cells. PLoS One. 2012; 7(3):e32655.
    View in: PubMed
  8. Meshulam T, Breen MR, Liu L, Parton RG, Pilch PF. Caveolins/caveolae protect adipocytes from fatty acid-mediated lipotoxicity. J Lipid Res. 2011 Aug; 52(8):1526-32.
    View in: PubMed
  9. Pilch PF, Liu L. Fat caves: caveolae, lipid trafficking and lipid metabolism in adipocytes. Trends Endocrinol Metab. 2011 Aug; 22(8):318-24.
    View in: PubMed
  10. Kandror KV, Pilch PF. The sugar is sIRVed: sorting Glut4 and its fellow travelers. Traffic. 2011 Jun; 12(6):665-71.
    View in: PubMed
  11. Howes MT, Kirkham M, Riches J, Cortese K, Walser PJ, Simpson F, Hill MM, Jones A, Lundmark R, Lindsay MR, Hernandez-Deviez DJ, Hadzic G, McCluskey A, Bashir R, Liu L, Pilch P, McMahon H, Robinson PJ, Hancock JF, Mayor S, Parton RG. Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells. J Cell Biol. 2010 Aug 23; 190(4):675-91.
    View in: PubMed
  12. Simard JR, Meshulam T, Pillai BK, Kirber MT, Brunaldi K, Xu S, Pilch PF, Hamilton JA. Caveolins sequester FA on the cytoplasmic leaflet of the plasma membrane, augment triglyceride formation, and protect cells from lipotoxicity. J Lipid Res. 2010 May; 51(5):914-22.
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  13. Jedrychowski MP, Gartner CA, Gygi SP, Zhou L, Herz J, Kandror KV, Pilch PF. Proteomic analysis of GLUT4 storage vesicles reveals LRP1 to be an important vesicle component and target of insulin signaling. J Biol Chem. 2010 Jan 1; 285(1):104-14.
    View in: PubMed
  14. Chao LC, Wroblewski K, Zhang Z, Pei L, Vergnes L, Ilkayeva OR, Ding SY, Reue K, Watt MJ, Newgard CB, Pilch PF, Hevener AL, Tontonoz P. Insulin resistance and altered systemic glucose metabolism in mice lacking Nur77. Diabetes. 2009 Dec; 58(12):2788-96.
    View in: PubMed
  15. Bastiani M, Liu L, Hill MM, Jedrychowski MP, Nixon SJ, Lo HP, Abankwa D, Luetterforst R, Fernandez-Rojo M, Breen MR, Gygi SP, Vinten J, Walser PJ, North KN, Hancock JF, Pilch PF, Parton RG. MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes. J Cell Biol. 2009 Jun 29; 185(7):1259-73.
    View in: PubMed
  16. Liu L, Brown D, McKee M, Lebrasseur NK, Yang D, Albrecht KH, Ravid K, Pilch PF. Deletion of Cavin/PTRF causes global loss of caveolae, dyslipidemia, and glucose intolerance. Cell Metab. 2008 Oct; 8(4):310-7.
    View in: PubMed
  17. Pilch PF. The mass action hypothesis: formation of Glut4 storage vesicles, a tissue-specific, regulated exocytic compartment. Acta Physiol (Oxf). 2008 Jan; 192(1):89-101.
    View in: PubMed
  18. Liu L, Pilch PF. A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organization. J Biol Chem. 2008 Feb 15; 283(7):4314-22.
    View in: PubMed
  19. Saito T, Jones CC, Huang S, Czech MP, Pilch PF. The interaction of Akt with APPL1 is required for insulin-stimulated Glut4 translocation. J Biol Chem. 2007 Nov 2; 282(44):32280-7.
    View in: PubMed
  20. Chao LC, Zhang Z, Pei L, Saito T, Tontonoz P, Pilch PF. Nur77 coordinately regulates expression of genes linked to glucose metabolism in skeletal muscle. Mol Endocrinol. 2007 Sep; 21(9):2152-63.
    View in: PubMed
  21. Pilch PF, Souto RP, Liu L, Jedrychowski MP, Berg EA, Costello CE, Gygi SP. Cellular spelunking: exploring adipocyte caveolae. J Lipid Res. 2007 Oct; 48(10):2103-11.
    View in: PubMed
  22. Coderre L, Vallega GA, Pilch PF, Chipkin SR. Regulation of glycogen concentration and glycogen synthase activity in skeletal muscle of insulin-resistant rats. Arch Biochem Biophys. 2007 Aug 1; 464(1):144-50.
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  23. Liu L, Jedrychowski MP, Gygi SP, Pilch PF. Role of insulin-dependent cortical fodrin/spectrin remodeling in glucose transporter 4 translocation in rat adipocytes. Mol Biol Cell. 2006 Oct; 17(10):4249-56.
    View in: PubMed
  24. Pilch PF, Bergenhem N. Pharmacological targeting of adipocytes/fat metabolism for treatment of obesity and diabetes. Mol Pharmacol. 2006 Sep; 70(3):779-85.
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  25. Kandror KV, Pilch PF. Isolation of GLUT4 storage vesicles. Curr Protoc Cell Biol. 2006 Apr; Chapter 3:Unit 3.20.
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  26. Meshulam T, Simard JR, Wharton J, Hamilton JA, Pilch PF. Role of caveolin-1 and cholesterol in transmembrane fatty acid movement. Biochemistry. 2006 Mar 7; 45(9):2882-93.
    View in: PubMed
  27. Gross DN, Miyoshi H, Hosaka T, Zhang HH, Pino EC, Souza S, Obin M, Greenberg AS, Pilch PF. Dynamics of lipid droplet-associated proteins during hormonally stimulated lipolysis in engineered adipocytes: stabilization and lipid droplet binding of adipocyte differentiation-related protein/adipophilin. Mol Endocrinol. 2006 Feb; 20(2):459-66.
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  28. Hosaka T, Brooks CC, Presman E, Kim SK, Zhang Z, Breen M, Gross DN, Sztul E, Pilch PF. p115 Interacts with the GLUT4 vesicle protein, IRAP, and plays a critical role in insulin-stimulated GLUT4 translocation. Mol Biol Cell. 2005 Jun; 16(6):2882-90.
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  29. Wharton J, Meshulam T, Vallega G, Pilch P. Dissociation of insulin receptor expression and signaling from caveolin-1 expression. J Biol Chem. 2005 Apr 8; 280(14):13483-6.
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  30. Wang YL, Guo W, Zang Y, Yaney GC, Vallega G, Getty-Kaushik L, Pilch P, Kandror K, Corkey BE. Acyl coenzyme a synthetase regulation: putative role in long-chain acyl coenzyme a partitioning. Obes Res. 2004 Nov; 12(11):1781-8.
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  31. Gross DN, Farmer SR, Pilch PF. Glut4 storage vesicles without Glut4: transcriptional regulation of insulin-dependent vesicular traffic. Mol Cell Biol. 2004 Aug; 24(16):7151-62.
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  32. Tortorella LL, Lin CB, Pilch PF. ERK6 is expressed in a developmentally regulated manner in rodent skeletal muscle. Biochem Biophys Res Commun. 2003 Jun 20; 306(1):163-8.
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  33. Tojo H, Kaieda I, Hattori H, Katayama N, Yoshimura K, Kakimoto S, Fujisawa Y, Presman E, Brooks CC, Pilch PF. The Formin family protein, formin homolog overexpressed in spleen, interacts with the insulin-responsive aminopeptidase and profilin IIa. Mol Endocrinol. 2003 Jul; 17(7):1216-29.
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  34. Souto RP, Vallega G, Wharton J, Vinten J, Tranum-Jensen J, Pilch PF. Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling. J Biol Chem. 2003 May 16; 278(20):18321-9.
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  35. Kamp F, Guo W, Souto R, Pilch PF, Corkey BE, Hamilton JA. Rapid flip-flop of oleic acid across the plasma membrane of adipocytes. J Biol Chem. 2003 Mar 7; 278(10):7988-95.
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  36. Tortorella LL, Pilch PF. C2C12 myocytes lack an insulin-responsive vesicular compartment despite dexamethasone-induced GLUT4 expression. Am J Physiol Endocrinol Metab. 2002 Sep; 283(3):E514-24.
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  37. Tortorella LL, Milasincic DJ, Pilch PF. Critical proliferation-independent window for basic fibroblast growth factor repression of myogenesis via the p42/p44 MAPK signaling pathway. J Biol Chem. 2001 Apr 27; 276(17):13709-17.
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  38. Martinez C, Vallega G, Pilch PF. Insulin-dependent phosphorylation of a 70-kDa protein in light microsomes from rat adipocytes. Biochem Biophys Res Commun. 2000 Oct 5; 276(3):1302-5.
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  39. Zhou M, Lin BZ, Coughlin S, Vallega G, Pilch PF. UCP-3 expression in skeletal muscle: effects of exercise, hypoxia, and AMP-activated protein kinase. Am J Physiol Endocrinol Metab. 2000 Sep; 279(3):E622-9.
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  40. Liu H, Kublaoui B, Pilch PF, Lee J. Insulin activation of mitogen-activated protein (MAP) kinase and Akt is phosphatidylinositol 3-kinase-dependent in rat adipocytes. Biochem Biophys Res Commun. 2000 Aug 11; 274(3):845-51.
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  41. Zhou M, Vallega G, Kandror KV, Pilch PF. Insulin-mediated translocation of GLUT-4-containing vesicles is preserved in denervated muscles. Am J Physiol Endocrinol Metab. 2000 Jun; 278(6):E1019-26.
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  42. Calera MR, Vallega G, Pilch PF. Dynamics of protein-tyrosine phosphatases in rat adipocytes. J Biol Chem. 2000 Mar 3; 275(9):6308-12.
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  43. Lee W, Ryu J, Souto RP, Pilch PF, Jung CY. Separation and partial characterization of three distinct intracellular GLUT4 compartments in rat adipocytes. Subcellular fractionation without homogenization. J Biol Chem. 1999 Dec 31; 274(53):37755-62.
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  44. Woldin CN, Hing FS, Lee J, Pilch PF, Shipley GG. Structural studies of the detergent-solubilized and vesicle-reconstituted insulin receptor. J Biol Chem. 1999 Dec 3; 274(49):34981-92.
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  45. Hamm JK, el Jack AK, Pilch PF, Farmer SR. Role of PPAR gamma in regulating adipocyte differentiation and insulin-responsive glucose uptake. Ann N Y Acad Sci. 1999 Nov 18; 892:134-45.
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  46. El-Jack AK, Kandror KV, Pilch PF. The formation of an insulin-responsive vesicular cargo compartment is an early event in 3T3-L1 adipocyte differentiation. Mol Biol Cell. 1999 May; 10(5):1581-94.
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  47. El-Jack AK, Hamm JK, Pilch PF, Farmer SR. Reconstitution of insulin-sensitive glucose transport in fibroblasts requires expression of both PPARgamma and C/EBPalpha. J Biol Chem. 1999 Mar 19; 274(12):7946-51.
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  48. Calera MR, Pilch PF. Induction of Akt-2 correlates with differentiation in Sol8 muscle cells. Biochem Biophys Res Commun. 1998 Oct 29; 251(3):835-41.
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  49. Lin B, Coughlin S, Pilch PF. Bidirectional regulation of uncoupling protein-3 and GLUT-4 mRNA in skeletal muscle by cold. Am J Physiol. 1998 Sep; 275(3 Pt 1):E386-91.
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  50. Zhou M, Sevilla L, Vallega G, Chen P, Palacin M, Zorzano A, Pilch PF, Kandror KV. Insulin-dependent protein trafficking in skeletal muscle cells. Am J Physiol. 1998 Aug; 275(2 Pt 1):E187-96.
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  51. Kummer JL, Calera MR, Pilch PF. Separation of IRS-1 and PI3-kinase from GLUT4 vesicles in rat skeletal muscle. Biochem Biophys Res Commun. 1998 May 8; 246(1):282-6.
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  52. Kandror KV, Pilch PF. Multiple endosomal recycling pathways in rat adipose cells. Biochem J. 1998 May 1; 331 ( Pt 3):829-35.
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  53. Calera MR, Martinez C, Liu H, Jack AK, Birnbaum MJ, Pilch PF. Insulin increases the association of Akt-2 with Glut4-containing vesicles. J Biol Chem. 1998 Mar 27; 273(13):7201-4.
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  54. Cushman SW, Goodyear LJ, Pilch PF, Ralston E, Galbo H, Ploug T, Kristiansen S, Klip A. Molecular mechanisms involved in GLUT4 translocation in muscle during insulin and contraction stimulation. Adv Exp Med Biol. 1998; 441:63-71.
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  55. Yaar M, Zhai S, Pilch PF, Doyle SM, Eisenhauer PB, Fine RE, Gilchrest BA. Binding of beta-amyloid to the p75 neurotrophin receptor induces apoptosis. A possible mechanism for Alzheimer's disease. J Clin Invest. 1997 Nov 1; 100(9):2333-40.
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  56. Lin BZ, Pilch PF, Kandror KV. Sortilin is a major protein component of Glut4-containing vesicles. J Biol Chem. 1997 Sep 26; 272(39):24145-7.
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  57. Lee J, Pilch PF, Shoelson SE, Scarlata SF. Conformational changes of the insulin receptor upon insulin binding and activation as monitored by fluorescence spectroscopy. Biochemistry. 1997 Mar 4; 36(9):2701-8.
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  58. Stephens JM, Lee J, Pilch PF. Tumor necrosis factor-alpha-induced insulin resistance in 3T3-L1 adipocytes is accompanied by a loss of insulin receptor substrate-1 and GLUT4 expression without a loss of insulin receptor-mediated signal transduction. J Biol Chem. 1997 Jan 10; 272(2):971-6.
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  59. Milasincic DJ, Calera MR, Farmer SR, Pilch PF. Stimulation of C2C12 myoblast growth by basic fibroblast growth factor and insulin-like growth factor 1 can occur via mitogen-activated protein kinase-dependent and -independent pathways. Mol Cell Biol. 1996 Nov; 16(11):5964-73.
    View in: PubMed
  60. Coderre L, Vallega GA, Pilch PF, Chipkin SR. In vivo effects of dexamethasone and sucrose on glucose transport (GLUT-4) protein tissue distribution. Am J Physiol. 1996 Oct; 271(4 Pt 1):E643-8.
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  61. Kandror KV, Pilch PF. The insulin-like growth factor II/mannose 6-phosphate receptor utilizes the same membrane compartments as GLUT4 for insulin-dependent trafficking to and from the rat adipocyte cell surface. J Biol Chem. 1996 Sep 6; 271(36):21703-8.
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  62. Kandror KV, Pilch PF. Compartmentalization of protein traffic in insulin-sensitive cells. Am J Physiol. 1996 Jul; 271(1 Pt 1):E1-14.
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  63. Stephens JM, Morrison RF, Pilch PF. The expression and regulation of STATs during 3T3-L1 adipocyte differentiation. J Biol Chem. 1996 May 3; 271(18):10441-4.
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  64. Tozzo E, Kahn BB, Pilch PF, Kandror KV. Glut4 is targeted to specific vesicles in adipocytes of transgenic mice overexpressing Glut4 selectively in adipose tissue. J Biol Chem. 1996 May 3; 271(18):10490-4.
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  65. Lee J, Shoelson SE, Pilch PF. Intermolecular phosphorylation between insulin holoreceptors does not stimulate substrate kinase activity. J Biol Chem. 1995 Dec 29; 270(52):31136-40.
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  66. Muñoz P, Rosemblatt M, Testar X, Palacín M, Thoidis G, Pilch PF, Zorzano A. The T-tubule is a cell-surface target for insulin-regulated recycling of membrane proteins in skeletal muscle. Biochem J. 1995 Dec 1; 312 ( Pt 2):393-400.
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  67. Coderre L, Kandror KV, Vallega G, Pilch PF. Identification and characterization of an exercise-sensitive pool of glucose transporters in skeletal muscle. J Biol Chem. 1995 Nov 17; 270(46):27584-8.
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  68. Stephens JM, Pilch PF. The metabolic regulation and vesicular transport of GLUT4, the major insulin-responsive glucose transporter. Endocr Rev. 1995 Aug; 16(4):529-46.
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  69. Kandror KV, Stephens JM, Pilch PF. Expression and compartmentalization of caveolin in adipose cells: coordinate regulation with and structural segregation from GLUT4. J Cell Biol. 1995 May; 129(4):999-1006.
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  70. Kandror KV, Coderre L, Pushkin AV, Pilch PF. Comparison of glucose-transporter-containing vesicles from rat fat and muscle tissues: evidence for a unique endosomal compartment. Biochem J. 1995 Apr 15; 307 ( Pt 2):383-90.
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  71. Kublaoui B, Lee J, Pilch PF. Dynamics of signaling during insulin-stimulated endocytosis of its receptor in adipocytes. J Biol Chem. 1995 Jan 6; 270(1):59-65.
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  72. Kandror KV, Yu L, Pilch PF. The major protein of GLUT4-containing vesicles, gp160, has aminopeptidase activity. J Biol Chem. 1994 Dec 9; 269(49):30777-80.
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  73. Kandror KV, Pilch PF. gp160, a tissue-specific marker for insulin-activated glucose transport. Proc Natl Acad Sci U S A. 1994 Aug 16; 91(17):8017-21.
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  74. Lee J, Pilch PF. The insulin receptor: structure, function, and signaling. Am J Physiol. 1994 Feb; 266(2 Pt 1):C319-34.
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  75. Kandror K, Pilch PF. Identification and isolation of glycoproteins that translocate to the cell surface from GLUT4-enriched vesicles in an insulin-dependent fashion. J Biol Chem. 1994 Jan 7; 269(1):138-42.
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  76. Pilch PF, Wilkinson W, Garvey WT, Ciaraldi TP, Hueckstaedt TP, Olefsky JM. Insulin-responsive human adipocytes express two glucose transporter isoforms and target them to different vesicles. J Clin Endocrinol Metab. 1993 Jul; 77(1):286-9.
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  77. Thoidis G, Kotliar N, Pilch PF. Immunological analysis of GLUT4-enriched vesicles. Identification of novel proteins regulated by insulin and diabetes. J Biol Chem. 1993 Jun 5; 268(16):11691-6.
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  78. Lee J, O'Hare T, Pilch PF, Shoelson SE. Insulin receptor autophosphorylation occurs asymmetrically. J Biol Chem. 1993 Feb 25; 268(6):4092-8.
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  79. Shoelson SE, Lee J, Lynch CS, Backer JM, Pilch PF. BpaB25 insulins. Photoactivatable analogues that quantitatively cross-link, radiolabel, and activate the insulin receptor. J Biol Chem. 1993 Feb 25; 268(6):4085-91.
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  80. Mischoulon D, Rana B, Kotliar N, Pilch PF, Bucher NL, Farmer SR. Differential regulation of glucose transporter 1 and 2 mRNA expression by epidermal growth factor and transforming growth factor-beta in rat hepatocytes. J Cell Physiol. 1992 Nov; 153(2):288-96.
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  81. Coderre L, Monfar MM, Chen KS, Heydrick SJ, Kurowski TG, Ruderman NB, Pilch PF. Alteration in the expression of GLUT-1 and GLUT-4 protein and messenger RNA levels in denervated rat muscles. Endocrinology. 1992 Oct; 131(4):1821-5.
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  82. Kotliar N, Pilch PF. Expression of the glucose transporter isoform GLUT 4 is insufficient to confer insulin-regulatable hexose uptake to cultured muscle cells. Mol Endocrinol. 1992 Mar; 6(3):337-45.
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  83. Goldfine ID, Pilch P. Insulin secretion and action and diabetes mellitus. J Cell Biochem. 1992 Jan; 48(1):1-2.
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  84. Shoelson SE, Boni-Schnetzler M, Pilch PF, Kahn CR. Autophosphorylation within insulin receptor beta-subunits can occur as an intramolecular process. Biochemistry. 1991 Aug 6; 30(31):7740-6.
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  85. Del Vecchio RL, Pilch PF. Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles. J Biol Chem. 1991 Jul 15; 266(20):13278-83.
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  86. Sinha MK, Raineri-Maldonado C, Buchanan C, Pories WJ, Carter-Su C, Pilch PF, Caro JF. Adipose tissue glucose transporters in NIDDM. Decreased levels of muscle/fat isoform. Diabetes. 1991 Apr; 40(4):472-7.
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  87. Dohm GL, Elton CW, Friedman JE, Pilch PF, Pories WJ, Atkinson SM, Caro JF. Decreased expression of glucose transporter in muscle from insulin-resistant patients. Am J Physiol. 1991 Mar; 260(3 Pt 1):E459-63.
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  88. Treadway JL, Morrison BD, Wemmie JA, Frias I, O'Hare T, Pilch PF, Pessin JE. The endogenous functional turkey erythrocyte and rat liver insulin receptor is an alpha 2 beta 2 heterotetrameric complex. Biochem J. 1990 Oct 1; 271(1):99-105.
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  89. Strout HV, Vicario PP, Biswas C, Saperstein R, Brady EJ, Pilch PF, Berger J. Vanadate treatment of streptozotocin diabetic rats restores expression of the insulin-responsive glucose transporter in skeletal muscle. Endocrinology. 1990 May; 126(5):2728-32.
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  90. Kasanicki MA, Pilch PF. Regulation of glucose-transporter function. Diabetes Care. 1990 Mar; 13(3):219-27.
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  91. O'Hare T, Pilch PF. Intrinsic kinase activity of the insulin receptor. Int J Biochem. 1990; 22(4):315-24.
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  92. Pilch PF. Glucose transporters: what's in a name? Endocrinology. 1990 Jan; 126(1):3-5.
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  93. Vilaró S, Palacín M, Pilch PF, Testar X, Zorzano A. Expression of an insulin-regulatable glucose carrier in muscle and fat endothelial cells. Nature. 1989 Dec 14; 342(6251):798-800.
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  94. Del Vecchio RL, Pilch PF. Insulin stimulates the tyrosine phosphorylation of a 165 kDa protein that is associated with microsomal membranes of rat adipocytes. Biochim Biophys Acta. 1989 Nov 17; 986(1):41-6.
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  95. Zorzano A, Wilkinson W, Kotliar N, Thoidis G, Wadzinkski BE, Ruoho AE, Pilch PF. Insulin-regulated glucose uptake in rat adipocytes is mediated by two transporter isoforms present in at least two vesicle populations. J Biol Chem. 1989 Jul 25; 264(21):12358-63.
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  96. Berger J, Biswas C, Vicario PP, Strout HV, Saperstein R, Pilch PF. Decreased expression of the insulin-responsive glucose transporter in diabetes and fasting. Nature. 1989 Jul 6; 340(6228):70-2.
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  97. Fukumoto H, Kayano T, Buse JB, Edwards Y, Pilch PF, Bell GI, Seino S. Cloning and characterization of the major insulin-responsive glucose transporter expressed in human skeletal muscle and other insulin-responsive tissues. J Biol Chem. 1989 May 15; 264(14):7776-9.
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  98. Waugh SM, DiBella EE, Pilch PF. Isolation of a proteolytically derived domain of the insulin receptor containing the major site of cross-linking/binding. Biochemistry. 1989 Apr 18; 28(8):3448-55.
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  99. Waugh SM, Pilch PF. Insulin binding changes the interface region between alpha subunits of the insulin receptor. Biochemistry. 1989 Mar 21; 28(6):2722-7.
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  100. Goldstein RH, Poliks CF, Pilch PF, Smith BD, Fine A. Stimulation of collagen formation by insulin and insulin-like growth factor I in cultures of human lung fibroblasts. Endocrinology. 1989 Feb; 124(2):964-70.
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  101. O'Hare T, Pilch PF. Intrinsic kinase activity of the insulin receptor. The intact (alpha 2 beta 2) insulin receptor from rat liver contains a kinase domain with greater intrinsic activity than the intact insulin receptor from human placenta. J Biol Chem. 1989 Jan 5; 264(1):602-10.
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  102. James DE, Pilch PF. Fractionation of endocytic vesicles and glucose-transporter-containing vesicles in rat adipocytes. Biochem J. 1988 Dec 15; 256(3):725-32.
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  103. Roome J, O'Hare T, Pilch PF, Brautigan DL. Protein phosphotyrosine phosphatase purified from the particulate fraction of human placenta dephosphorylates insulin and growth-factor receptors. Biochem J. 1988 Dec 1; 256(2):493-500.
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  104. Tapson VF, Boni-Schnetzler M, Pilch PF, Center DM, Berman JS. Structural and functional characterization of the human T lymphocyte receptor for insulin-like growth factor I in vitro. J Clin Invest. 1988 Sep; 82(3):950-7.
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  105. O'Hare T, Pilch PF. Separation and characterization of three insulin receptor species that differ in subunit composition. Biochemistry. 1988 Jul 26; 27(15):5693-700.
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  106. Zorzano A, James DE, Ruderman NB, Pilch PF. Insulin-like growth factor I binding and receptor kinase in red and white muscle. FEBS Lett. 1988 Jul 18; 234(2):257-62.
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  107. Böni-Schnetzler M, Kaligian A, DelVecchio R, Pilch PF. Ligand-dependent intersubunit association within the insulin receptor complex activates its intrinsic kinase activity. J Biol Chem. 1988 May 15; 263(14):6822-8.
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  108. James DE, Brown R, Navarro J, Pilch PF. Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein. Nature. 1988 May 12; 333(6169):183-5.
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  109. Aiello LP, Shia MA, Robinson GS, Pilch PF, Farmer SR. Characterization and hepatic expression of rat alpha 1-inhibitor III mRNA. J Biol Chem. 1988 Mar 15; 263(8):4013-22.
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  110. Böni-Schnetzler M, Pilch PF. Mechanism of epidermal growth factor receptor autophosphorylation and high-affinity binding. Proc Natl Acad Sci U S A. 1987 Nov; 84(22):7832-6.
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  111. James DE, Lederman L, Pilch PF. Purification of insulin-dependent exocytic vesicles containing the glucose transporter. J Biol Chem. 1987 Aug 25; 262(24):11817-24.
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  112. Böni-Schnetzler M, Scott W, Waugh SM, DiBella E, Pilch PF. The insulin receptor. Structural basis for high affinity ligand binding. J Biol Chem. 1987 Jun 15; 262(17):8395-401.
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  113. Cousin JL, Samson M, Pilch PF, Fehlmann M. Internalization of insulin receptors and HLA antigens in human hepatoma cells. Biochem J. 1987 Mar 1; 242(2):403-10.
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  114. Böni-Schnetzler M, Rubin JB, Pilch PF. Structural requirements for the transmembrane activation of the insulin receptor kinase. J Biol Chem. 1986 Nov 15; 261(32):15281-7.
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  115. James DE, Zorzano A, Böni-Schnetzler M, Nemenoff RA, Powers A, Pilch PF, Ruderman NB. Intrinsic differences of insulin receptor kinase activity in red and white muscle. J Biol Chem. 1986 Nov 15; 261(32):14939-44.
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  116. Aiello LP, Wessling-Resnick M, Pilch PF. Dipeptide metalloendoprotease substrates are glucose transport inhibitors and membrane structure perturbants. Biochemistry. 1986 Jul 1; 25(13):3944-50.
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  117. Pilch PF, O'Hare T, Rubin J, Boni-Schnetzler M. The ligand binding subunit of the insulin-like growth factor 1 receptor has properties of a peripheral membrane protein. Biochem Biophys Res Commun. 1986 Apr 14; 136(1):45-50.
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  118. Helmy S, Porter-Jordan K, Dawidowicz EA, Pilch P, Schwartz AL, Fine RE. Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique. Cell. 1986 Feb 14; 44(3):497-506.
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  119. Wessling M, Pilch PF. Characterization and solubilization of the cytochalasin B binding component from human placental microsomes. Biochim Biophys Acta. 1984 Oct 17; 777(1):123-32.
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  120. Campbell C, Squicciarini J, Shia M, Pilch PF, Fine RE. Identification of a protein kinase as an intrinsic component of rat liver coated vesicles. Biochemistry. 1984 Sep 11; 23(19):4420-6.
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  121. Shia MA, Rubin JB, Pilch PF. The insulin receptor protein kinase. Physicochemical requirements for activity. J Biol Chem. 1983 Dec 10; 258(23):14450-5.
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  122. Rubin JB, Shia MA, Pilch PF. Stimulation of tyrosine-specific phosphorylation in vitro by insulin-like growth factor I. Nature. 1983 Sep 29-Oct 5; 305(5933):438-40.
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  123. Axelrod JD, Pilch PF. Unique cytochalasin B binding characteristics of the hepatic glucose carrier. Biochemistry. 1983 Apr 26; 22(9):2222-7.
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  124. Shia MA, Pilch PF. The beta subunit of the insulin receptor is an insulin-activated protein kinase. Biochemistry. 1983 Feb 15; 22(4):717-21.
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  125. Pilch PF, Shia MA, Benson RJ, Fine RE. Coated vesicles participate in the receptor-mediated endocytosis of insulin. J Cell Biol. 1983 Jan; 96(1):133-8.
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  126. Pilch PF. Modification of the insulin receptor by diethyl pyrocarbonate: effect on insulin binding and action. Biochemistry. 1982 Oct 26; 21(22):5638-44.
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  127. Massague J, Pilch PF, Czech MP. A unique proteolytic cleavage site on the beta subunit of the insulin receptor. J Biol Chem. 1981 Apr 10; 256(7):3182-90.
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  128. Pilch PF, Axelrod JD, Colello J, Czech MP. Unimpaired signal transduction by the adipocyte insulin receptor following its partial proteolytic fragmentation. J Biol Chem. 1981 Feb 25; 256(4):1570-5.
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  129. Johnson GL, MacAndrew VI, Pilch PF. Identification of the glucagon receptor in rat liver membranes by photoaffinity crosslinking. Proc Natl Acad Sci U S A. 1981 Feb; 78(2):875-8.
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  130. Carter-Su C, Pilch PF, Czech MP. Chromatographic resolution of insulin receptor from insulin-sensitive D-glucose transporter of adipocyte plasma membranes. Biochemistry. 1981 Jan 6; 20(1):216-21.
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  131. Pilch PF, Czech MP. Hormone binding alters the conformation of the insulin receptor. Science. 1980 Dec 5; 210(4474):1152-3.
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  132. Massague J, Pilch PF, Czech MP. Electrophoretic resolution of three major insulin receptor structures with unique subunit stoichiometries. Proc Natl Acad Sci U S A. 1980 Dec; 77(12):7137-41.
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  133. Pillion DJ, Carter-Su CA, Pilch PF, Czech MP. Isolation of adipocyte plasma membrane antigens by immunoaffinity chromatography. Insulinomimetic antibodies do not bind directly to the insulin receptor or the glucose transport system. J Biol Chem. 1980 Oct 10; 255(19):9168-76.
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  134. Czech MP, Malbon CC, Kerman K, Gitomer W, Pilch PF. Effect of thyroid status on insulin action in rat adipocytes and skeletal muscle. J Clin Invest. 1980 Sep; 66(3):574-82.
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  135. Heinrich J, Pilch PF, Czech MP. Purification of the adipocyte insulin receptor by immunoaffinity chromatography. J Biol Chem. 1980 Feb 25; 255(4):1732-7.
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  136. Pilch PF, Czech MP. The subunit structure of the high affinity insulin receptor. Evidence for a disulfide-linked receptor complex in fat cell and liver plasma membranes. J Biol Chem. 1980 Feb 25; 255(4):1722-31.
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  137. Pilch PF, Thompson PA, Czech MP. Coordinate modulation of D-glucose transport activity and bilayer fluidity in plasma membranes derived from control and insulin-treated adipocytes. Proc Natl Acad Sci U S A. 1980 Feb; 77(2):915-8.
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  138. Pilch PF, Czech MP. Hexose transport in adipocytes: stimulation by insulin in the absence of intact receptor. Ann N Y Acad Sci. 1980; 358:356.
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  139. Czech MP, Massague J, Pilch PF, Carter-Su C. Structural features of the insulin effector system: relation to hexose transport activation. Ann N Y Acad Sci. 1980; 358:282-91.
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  140. Pilch PF, Czech MP. Interaction of cross-linking agents with the insulin effector system of isolated fat cells. Covalent linkage of 125I-insulin to a plasma membrane receptor protein of 140,000 daltons. J Biol Chem. 1979 May 10; 254(9):3375-81.
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  141. Pilch PF, Somerville RL. Fluorine-containing analogues of intermediates in the Shikimate pathway. Biochemistry. 1976 Nov 30; 15(24):5315-20.
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