Gary Gilbert | Profiles RNS

Gary Gilbert, MD

Adjunct Associate Professor

Boston University Chobanian & Avedisian School of Medicine

Medicine

Hematology & Medical Oncology

MD, Loma Linda University

Websites

	LinkedIn
	Harvard Catalyst
	Little Sparrows Technologies

Dr. Gary Gilbert, M.D. is the Deputy Associate Chief of Staff for Research and Development at the VA Boston Healthcare System. In his capacity, he oversees the basic science activities at all three campuses and has charge of major initiatives to import new technical capabilities and build a major expansion of the animal facilities He also serves as a member of BVARI’s Board of Directors. Dr. Gilbert is Associate Physician in Medicine, Brigham and Women’s Hospital and the Associate Professor of Medicine, Harvard medical School.

His research interests target understanding the mechanisms through which activity of blood coagulation proteins is regulated on cell membranes. He has four categories of research in his laboratory:

1. To study the characterization of the mechanism through which lactadherin binds to membranes and functions as an anticoagulant in vitro. This includes structure-function studies to identify essential features of the interaction.

2. To utilize lactadherin and probes derived from lactadherin to identify procoagulant membranes in vivo and to probe the importance.

3. To identify the functional amino acids on the membrane-interactive C1 and C2 domains of factor VIII. This project may lead to engineering factor VIII so that the product molecule has higher specific activity and a slower rate of clearance.

4. To seek to determine through what mechanisms phospholipid molecules activate the factor VIIIa-factor IXa enzyme complex. This information will provide improved basic insight and, possibly, a novel approach to treatment of hemophilia patients that have developed inhibitory antibodies to factor VIII.

VA Boston Healthcare System

NIH RePORTER Research
VA Research

Title
Platelet-Dependent Activity of Factor VIII in Mild and Moderat Hemophilia (PI)
Endothelial Topography, Phosphatidylserine, and Procoagulant Activity (PI)

Yr	Title	Project-Sub Proj
2023	Endothelial topography, phosphatidylserine, and procoagulant activity	5I01BX001352-11
2022	Endothelial topography, phosphatidylserine, and procoagulant activity	5I01BX001352-10
2021	Endothelial topography, phosphatidylserine, and procoagulant activity	2I01BX001352-09A1
2020	Endothelial topography, phosphatidylserine, and procoagulant activity	5I01BX001352-08
2019	Endothelial topography, phosphatidylserine, and procoagulant activity	5I01BX001352-07
2018	Endothelial topography, phosphatidylserine, and procoagulant activity	5I01BX001352-06
2017	Endothelial topography, phosphatidylserine, and procoagulant activity	2I01BX001352-05
2015	Endothelial cell phosphatidylserine, topography, and procoagulant activity	5I01BX001352-04
2014	Endothelial cell phosphatidylserine, topography, and procoagulant activity	5I01BX001352-03
2013	Endothelial cell phosphatidylserine, topography, and procoagulant activity	5I01BX001352-02

Showing 10 of 25 results. Show All Results

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis Copy PMIDs To Clipboard

Carman CV, Nikova DN, Sakurai Y, Shi J, Novakovic VA, Rasmussen JT, Lam WA, Gilbert GE. Membrane curvature and PS localize coagulation proteins to filopodia and retraction fibers of endothelial cells. Blood Adv. 2023 Jan 10; 7(1):60-72. PMID: 35849711; PMCID: PMC9827038; DOI: 10.1182/bloodadvances.2021006870;

Gilbert GE. Factor VIII-antibody structure and membrane binding. Blood. 2021 05 27; 137(21):2866-2868. PMID: 34042979; PMCID: PMC8160506; DOI: 10.1182/blood.2021010947;

Novakovic VA, Gilbert GE. Procoagulant activities of skeletal and cardiac muscle myosin depend on contaminating phospholipid. Blood. 2020 11 19; 136(21):2469-2472. PMID: 32604409; PMCID: PMC7685214; DOI: 10.1182/blood.2020005930;

Chatterjee M, Meeks S, Novakovic VA, Gilbert GE. Discordance between platelet-supported and vesicle-supported factor VIII activity in the presence of anti-C2 domain inhibitory antibodies. J Thromb Haemost. 2020 12; 18(12):3184-3193. PMID: 32558078

Shi Q, Carman CV, Chen Y, Sage PT, Xue F, Liang XM, Gilbert GE. Unexpected enhancement of FVIII immunogenicity by endothelial expression in lentivirus-transduced and transgenic mice. Blood Adv. 2020 05 26; 4(10):2272-2285. PMID: 32453842; PMCID: PMC7252558; DOI: 10.1182/bloodadvances.2020001468;

Gilbert GE. The evolving understanding of factor VIII binding sites and implications for the treatment of hemophilia A. Blood Rev. 2019 01; 33:1-5. PMID: 29866493; DOI: 10.1016/j.blre.2018.05.001;

Sakurai Y, Hardy ET, Ahn B, Tran R, Fay ME, Ciciliano JC, Mannino RG, Myers DR, Qiu Y, Carden MA, Baldwin WH, Meeks SL, Gilbert GE, Jobe SM, Lam WA. A microengineered vascularized bleeding model that integrates the principal components of hemostasis. Nat Commun. 2018 02 06; 9(1):509. PMID: 29410404; PMCID: PMC5802762; DOI: 10.1038/s41467-018-02990-x;

Gilbert GE. Inhibitory antibodies against factor VIII C1 domain. Blood. 2016 10 20; 128(16):2001-2002. PMID: 28157676; PMCID: PMC5073179; DOI: 10.1182/blood-2016-08-727818;

Gao C, Xie R, Yu C, Ma R, Dong W, Meng H, Zhang Y, Si Y, Zhang Z, Novakovic V, Zhang Y, Kou J, Bi Y, Li B, Xie R, Gilbert GE, Zhou J, Shi J. Thrombotic Role of Blood and Endothelial Cells in Uremia through Phosphatidylserine Exposure and Microparticle Release. PLoS One. 2015; 10(11):e0142835. PMID: 26580207; PMCID: PMC4646287; DOI: 10.1371/journal.pone.0142835;

Gilbert GE, Novakovic VA, Shi J, Rasmussen J, Pipe SW. Platelet binding sites for factor VIII in relation to fibrin and phosphatidylserine. Blood. 2015 Sep 03; 126(10):1237-44. PMID: 26162408; PMCID: PMC4559935; DOI: 10.1182/blood-2015-01-620245;

Showing 10 of 58 results. Show More

Pantazatos D, Gessner CR, Woods VL, Gilbert GE. Changes in the Factor VIII C2 domain upon membrane binding determined by hydrogen-deuterium exchange MS. Biochem J. 2014 Aug 01; 461(3):443-51. PMID: 24814520; DOI: 10.1042/BJ20140121;

Gilbert GE. Factor VIII inhibitor epitopes and an enigma. Blood. 2013 Dec 19; 122(26):4160-1. PMID: 24357711; DOI: 10.1182/blood-2013-10-531822;

Nyegaard S, Novakovic VA, Rasmussen JT, Gilbert GE. Lactadherin inhibits secretory phospholipase A2 activity on pre-apoptotic leukemia cells. PLoS One. 2013; 8(10):e77143. PMID: 24194865; PMCID: PMC3806724; DOI: 10.1371/journal.pone.0077143;

Gao C, Xie R, Li W, Zhou J, Liu S, Cao F, Liu Y, Ma R, Si Y, Liu Y, Bi Y, Gilbert GE, Shi J. Endothelial cell phagocytosis of senescent neutrophils decreases procoagulant activity. Thromb Haemost. 2013 Jun; 109(6):1079-90. PMID: 23571768; DOI: 10.1160/TH12-12-0894;

Gilbert GE, Novakovic VA, Kaufman RJ, Miao H, Pipe SW. Conservative mutations in the C2 domains of factor VIII and factor V alter phospholipid binding and cofactor activity. Blood. 2012 Aug 30; 120(9):1923-32. PMID: 22613792; PMCID: PMC3433094; DOI: 10.1182/blood-2012-01-408245;

Nuzzio KM, Cullinan DB, Novakovic VA, Boettcher JM, Rienstra CM, Gilbert GE, Baleja JD. Backbone resonance assignments of the C2 domain of coagulation factor VIII. Biomol NMR Assign. 2013 Apr; 7(1):31-4. PMID: 22392338; DOI: 10.1007/s12104-012-9370-5;

Gao C, Xie R, Yu C, Wang Q, Shi F, Yao C, Xie R, Zhou J, Gilbert GE, Shi J. Procoagulant activity of erythrocytes and platelets through phosphatidylserine exposure and microparticles release in patients with nephrotic syndrome. Thromb Haemost. 2012 Apr; 107(4):681-9. PMID: 22370875

Xie R, Gao C, Li W, Zhu J, Novakovic V, Wang J, Ma R, Zhou J, Gilbert GE, Shi J. Phagocytosis by macrophages and endothelial cells inhibits procoagulant and fibrinolytic activity of acute promyelocytic leukemia cells. Blood. 2012 Mar 08; 119(10):2325-34. PMID: 22138513; DOI: 10.1182/blood-2011-06-362186;

Otzen DE, Blans K, Wang H, Gilbert GE, Rasmussen JT. Lactadherin binds to phosphatidylserine-containing vesicles in a two-step mechanism sensitive to vesicle size and composition. Biochim Biophys Acta. 2012 Apr; 1818(4):1019-27. PMID: 21920348; DOI: 10.1016/j.bbamem.2011.08.032;

Gilbert GE, Mast AE. Curbing an inhibitor for hemostasis. Blood. 2011 May 19; 117(20):5277-8. PMID: 21596863; DOI: 10.1182/blood-2011-03-342725;

Novakovic VA, Cullinan DB, Wakabayashi H, Fay PJ, Baleja JD, Gilbert GE. Membrane-binding properties of the Factor VIII C2 domain. Biochem J. 2011 Apr 01; 435(1):187-96. PMID: 21210768; PMCID: PMC3670696; DOI: 10.1042/BJ20101797;

Lü J, Pipe SW, Miao H, Jacquemin M, Gilbert GE. A membrane-interactive surface on the factor VIII C1 domain cooperates with the C2 domain for cofactor function. Blood. 2011 Mar 17; 117(11):3181-9. PMID: 21156843; DOI: 10.1182/blood-2010-08-301663;

Fu Y, Zhou J, Li H, Cao F, Su Y, Fan S, Li Y, Wang S, Li L, Gilbert GE, Shi J. Daunorubicin induces procoagulant activity of cultured endothelial cells through phosphatidylserine exposure and microparticles release. Thromb Haemost. 2010 Dec; 104(6):1235-41. PMID: 20886178; DOI: 10.1160/TH10-02-0102;

Hou J, Fu Y, Zhou J, Li W, Xie R, Cao F, Gilbert GE, Shi J. Lactadherin functions as a probe for phosphatidylserine exposure and as an anticoagulant in the study of stored platelets. Vox Sang. 2011 Feb; 100(2):187-95. PMID: 20738837

Zhou J, Shi J, Hou J, Cao F, Zhang Y, Rasmussen JT, Heegaard CW, Gilbert GE. Phosphatidylserine exposure and procoagulant activity in acute promyelocytic leukemia. J Thromb Haemost. 2010 Apr; 8(4):773-82. PMID: 20102487

Shcherbina A, Cooley J, Lutskiy MI, Benarafa C, Gilbert GE, Remold-O'Donnell E. WASP plays a novel role in regulating platelet responses dependent on alphaIIbbeta3 integrin outside-in signalling. Br J Haematol. 2010 Feb; 148(3):416-27. PMID: 19863535; PMCID: PMC2810352; DOI: 10.1111/j.1365-2141.2009.07959.x;

Zhou J, Zheng Y, Shi J, Lu C, Hou J, Yu H, Qiao X, Qi S, Gilbert GE. Daunorubicin induces procoagulant response through phosphatidylserine exposure in red blood cells. Thromb Res. 2010 Feb; 125(2):178-83. PMID: 19800661; DOI: 10.1016/j.thromres.2009.09.006;

Meems H, Meijer AB, Cullinan DB, Mertens K, Gilbert GE. Factor VIII C1 domain residues Lys 2092 and Phe 2093 contribute to membrane binding and cofactor activity. Blood. 2009 Oct 29; 114(18):3938-46. PMID: 19687511; DOI: 10.1182/blood-2009-01-197707;

Waehrens LN, Heegaard CW, Gilbert GE, Rasmussen JT. Bovine lactadherin as a calcium-independent imaging agent of phosphatidylserine expressed on the surface of apoptotic HeLa cells. J Histochem Cytochem. 2009 Oct; 57(10):907-14. PMID: 19546474; PMCID: PMC2746724; DOI: 10.1369/jhc.2009.953729;

Zhou J, Liu S, Ma M, Hou J, Yu H, Lu C, Gilbert GE, Shi J. Procoagulant activity and phosphatidylserine of amniotic fluid cells. Thromb Haemost. 2009 May; 101(5):845-51. PMID: 19404537

Gilbert GE. Where does von Willebrand factor go? Blood. 2008 Sep 01; 112(5):1549-50. PMID: 18725568

Shi J, Pipe SW, Rasmussen JT, Heegaard CW, Gilbert GE. Lactadherin blocks thrombosis and hemostasis in vivo: correlation with platelet phosphatidylserine exposure. J Thromb Haemost. 2008 Jul; 6(7):1167-74. PMID: 18485093

Yeung T, Gilbert GE, Shi J, Silvius J, Kapus A, Grinstein S. Membrane phosphatidylserine regulates surface charge and protein localization. Science. 2008 Jan 11; 319(5860):210-3. PMID: 18187657

Shao C, Novakovic VA, Head JF, Seaton BA, Gilbert GE. Crystal structure of lactadherin C2 domain at 1.7A resolution with mutational and computational analyses of its membrane-binding motif. J Biol Chem. 2008 Mar 14; 283(11):7230-41.View Related Profiles.

Seaton, Barbara
Gilbert, Gary
Head, James

PMID: 18160406

Shi J, Shi Y, Waehrens LN, Rasmussen JT, Heegaard CW, Gilbert GE. Lactadherin detects early phosphatidylserine exposure on immortalized leukemia cells undergoing programmed cell death. Cytometry A. 2006 Dec 01; 69(12):1193-201. PMID: 17123296

Gilbert GE. Use of microsphere-supported phospholipid membranes for analysis of protein-lipid interactions. Curr Protoc Cytom. 2005 Nov; Chapter 13:Unit13.3. PMID: 18770819

Grant MA, Baikeev RF, Gilbert GE, Rigby AC. Lysine 5 and phenylalanine 9 of the factor IX omega-loop interact with phosphatidylserine in a membrane-mimetic environment. Biochemistry. 2004 Dec 14; 43(49):15367-78. PMID: 15581349

Shi J, Heegaard CW, Rasmussen JT, Gilbert GE. Lactadherin binds selectively to membranes containing phosphatidyl-L-serine and increased curvature. Biochim Biophys Acta. 2004 Nov 17; 1667(1):82-90. PMID: 15533308

Phillips JE, Lord ST, Gilbert GE. Fibrin stimulates platelets to increase factor VIIIa binding site expression. J Thromb Haemost. 2004 Oct; 2(10):1806-15. PMID: 15456493

Shi J, Gilbert GE. Lactadherin inhibits enzyme complexes of blood coagulation by competing for phospholipid-binding sites. Blood. 2003 Apr 01; 101(7):2628-36. PMID: 12517809

Gilbert GE, Kaufman RJ, Arena AA, Miao H, Pipe SW. Four hydrophobic amino acids of the factor VIII C2 domain are constituents of both the membrane-binding and von Willebrand factor-binding motifs. J Biol Chem. 2002 Feb 22; 277(8):6374-81. PMID: 11698391

Shi J, Gilbert GE, Kokubo Y, Ohashi T. Role of the liver in regulating numbers of circulating neutrophils. Blood. 2001 Aug 15; 98(4):1226-30. PMID: 11493474

Blostein MD, Rigby AC, Furie BC, Furie B, Gilbert GE. Amphipathic helices support function of blood coagulation factor IXa. Biochemistry. 2000 Oct 03; 39(39):12000-6. PMID: 11009614

Bendetowicz AV, Wise RJ, Gilbert GE. Collagen-bound von Willebrand factor has reduced affinity for factor VIII. J Biol Chem. 1999 Apr 30; 274(18):12300-7. PMID: 10212199

Gilbert GE, Arena AA. Unsaturated phospholipid acyl chains are required to constitute membrane binding sites for factor VIII. Biochemistry. 1998 Sep 29; 37(39):13526-35. PMID: 9753438

Bendetowicz AV, Morris JA, Wise RJ, Gilbert GE, Kaufman RJ. Binding of factor VIII to von willebrand factor is enabled by cleavage of the von Willebrand factor propeptide and enhanced by formation of disulfide-linked multimers. Blood. 1998 Jul 15; 92(2):529-38. PMID: 9657753

Veeraraghavan S, Baleja JD, Gilbert GE. Structure and topography of the membrane-binding C2 domain of factor VIII in the presence of dodecylphosphocholine micelles. Biochem J. 1998 Jun 01; 332 ( Pt 2):549-55. PMID: 9601086

Gilbert GE, Arena AA. Partial activation of the factor VIIIa-factor IXa enzyme complex by dihexanoic phosphatidylserine at submicellar concentrations. Biochemistry. 1997 Sep 02; 36(35):10768-76. PMID: 9271508

Saenko EL, Shima M, Gilbert GE, Scandella D. Slowed release of thrombin-cleaved factor VIII from von Willebrand factor by a monoclonal and a human antibody is a novel mechanism for factor VIII inhibition. J Biol Chem. 1996 Nov 01; 271(44):27424-31. PMID: 8910322; DOI: 10.1074/jbc.271.44.27424;

Gilbert GE, Arena AA. Activation of the factor VIIIa-factor IXa enzyme complex of blood coagulation by membranes containing phosphatidyl-L-serine. J Biol Chem. 1996 May 10; 271(19):11120-5. PMID: 8626656

Scandella D, Gilbert GE, Shima M, Nakai H, Eagleson C, Felch M, Prescott R, Rajalakshmi KJ, Hoyer LW, Saenko E. Some factor VIII inhibitor antibodies recognize a common epitope corresponding to C2 domain amino acids 2248 through 2312, which overlap a phospholipid-binding site. Blood. 1995 Sep 01; 86(5):1811-9. PMID: 7544643

Gilbert GE, Arena AA. Phosphatidylethanolamine induces high affinity binding sites for factor VIII on membranes containing phosphatidyl-L-serine. J Biol Chem. 1995 Aug 04; 270(31):18500-5. PMID: 7629178

Gilbert GE, Baleja JD. Membrane-binding peptide from the C2 domain of factor VIII forms an amphipathic structure as determined by NMR spectroscopy. Biochemistry. 1995 Mar 07; 34(9):3022-31. PMID: 7893714

Gilbert GE, Drinkwater D. Specific membrane binding of factor VIII is mediated by O-phospho-L-serine, a moiety of phosphatidylserine. Biochemistry. 1993 Sep 21; 32(37):9577-85. PMID: 8373765

Bardelle C, Furie B, Furie BC, Gilbert GE. Membrane binding kinetics of factor VIII indicate a complex binding process. J Biol Chem. 1993 Apr 25; 268(12):8815-24. PMID: 8473326

Gilbert GE, Drinkwater D, Barter S, Clouse SB. Specificity of phosphatidylserine-containing membrane binding sites for factor VIII. Studies with model membranes supported by glass microspheres (lipospheres). J Biol Chem. 1992 Aug 05; 267(22):15861-8. PMID: 1639816

Gilbert GE, Sims PJ, Wiedmer T, Furie B, Furie BC, Shattil SJ. Platelet-derived microparticles express high affinity receptors for factor VIII. J Biol Chem. 1991 Sep 15; 266(26):17261-8. PMID: 1654328

Gilbert GE, Furie BC, Furie B. Binding of human factor VIII to phospholipid vesicles. J Biol Chem. 1990 Jan 15; 265(2):815-22. PMID: 2104832

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 58 publications over 29 distinct years, with a maximum of 5 publications in 2009

Bar chart showing 58 publications over 29 distinct years, with a maximum of 5 publications in 2009

Year	Publications
1990	1
1991	1
1992	1
1993	2
1995	3
1996	2
1997	1
1998	3
1999	1
2000	1
2001	2
2002	1
2004	3
2005	1
2006	1
2007	1
2008	3
2009	5
2010	4
2011	4
2012	3
2013	3
2014	1
2015	2
2016	1
2018	2
2020	3
2021	1
2023	1

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72 E. Concord St Building A
Boston MA 02118
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