Keywords
Last Name

James F. Head, PhD

TitleProfessor
InstitutionBoston University School of Medicine
DepartmentPhysiology & Biophysics
Address700 Albany St
Boston MA 02118
Phone(617) 638-4396
ORCID ORCID Icon0000-0002-6226-4146
Other Positions
TitleGraduate Faculty (Primary Mentor of Grad Students)
InstitutionBoston University School of Medicine, Graduate Medical Sciences

 Research Expertise & Professional Interests
Research in our lab focuses on characterizing the molecular details of protein-ligand and protein-protein interactions and the mechanisms by which changes in these interactions regulate physiological processes. These studies have concentrated mainly on the role of the calcium ion in mediating changes in protein-ligand interactions which subsequently may alter protein-protein interactions to modulate function. However, other protein-ligand interactions are also under study including enzyme-effector and protein-protein complexes. X-ray crystallography is a major tool used in these studies.

 NIH RePORTER Grants

Yr Title Project-Sub Proj Pubs
2001 MULTIPLE ANTIBIOTIC RESISTANCE REPRESSOR PROTEIN 3P41RR010888-05S1-27 222
2000 MULTIPLE ANTIBIOTIC RESISTANCE REPRESSOR PROTEIN 5P41RR010888-05-27 222
1999 MULTIPLE ANTIBIOTIC RESISTANCE REPRESSOR PROTEIN 5P41RR010888-04-27 222


 Self-Described Keywords
  • Calcium-binding proteins
  • Lung surfactant proteins
  • Protein crystallography
 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Farelli JD, Gumbart JC, Akey IV, Hempstead A, Amyot W, Head JF, McKnight CJ, Isberg RR, Akey CW. IcmQ in the Type 4b secretion system contains an NAD+ binding domain. Structure. 2013 Aug 6; 21(8):1361-73.
    View in: PubMed
  2. Duval V, McMurry LM, Foster K, Head JF, Levy SB. Mutational analysis of the multiple-antibiotic resistance regulator MarR reveals a ligand binding pocket at the interface between the dimerization and DNA binding domains. J Bacteriol. 2013 Aug; 195(15):3341-51.
    View in: PubMed
  3. van Eijk M, Rynkiewicz MJ, White MR, Hartshorn KL, Zou X, Schulten K, Luo D, Crouch EC, Cafarella TR, Head JF, Haagsman HP, Seaton BA. A unique sugar-binding site mediates the distinct anti-influenza activity of pig surfactant protein D. J Biol Chem. 2012 Aug 3; 287(32):26666-77.
    View in: PubMed
  4. Platonova O, Akey IV, Head JF, Akey CW. Crystal structure and function of human nucleoplasmin (npm2): a histone chaperone in oocytes and embryos. Biochemistry. 2011 Sep 20; 50(37):8078-89.
    View in: PubMed
  5. Shang F, Rynkiewicz MJ, McCormack FX, Wu H, Cafarella TM, Head JF, Seaton BA. Crystallographic complexes of surfactant protein A and carbohydrates reveal ligand-induced conformational change. J Biol Chem. 2011 Jan 7; 286(1):757-65.
    View in: PubMed
  6. Hartshorn KL, White MR, Rynkiewicz M, Sorensen G, Holmskov U, Head J, Crouch EC. Monoclonal antibody-assisted structure-function analysis of the carbohydrate recognition domain of surfactant protein D. Am J Physiol Lung Cell Mol Physiol. 2010 Sep; 299(3):L384-92.
    View in: PubMed
  7. Hartshorn KL, White MR, Smith K, Sorensen G, Kuroki Y, Holmskov U, Head J, Crouch EC. Increasing antiviral activity of surfactant protein d trimers by introducing residues from bovine serum collectins: dissociation of mannan-binding and antiviral activity. Scand J Immunol. 2010 Jul; 72(1):22-30.
    View in: PubMed
  8. Seaton BA, Crouch EC, McCormack FX, Head JF, Hartshorn KL, Mendelsohn R. Review: Structural determinants of pattern recognition by lung collectins. Innate Immun. 2010 Jun; 16(3):143-50.
    View in: PubMed
  9. Crouch E, Hartshorn K, Horlacher T, McDonald B, Smith K, Cafarella T, Seaton B, Seeberger PH, Head J. Recognition of mannosylated ligands and influenza A virus by human surfactant protein D: contributions of an extended site and residue 343. Biochemistry. 2009 Apr 21; 48(15):3335-45.
    View in: PubMed
  10. Raychaudhury S, Farelli JD, Montminy TP, Matthews M, Ménétret JF, Duménil G, Roy CR, Head JF, Isberg RR, Akey CW. Structure and function of interacting IcmR-IcmQ domains from a type IVb secretion system in Legionella pneumophila. Structure. 2009 Apr 15; 17(4):590-601.
    View in: PubMed
  11. Shi X, Shao C, Zhang X, Zambonelli C, Redfield AG, Head JF, Seaton BA, Roberts MF. Modulation of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C activity by mutations in the putative dimerization interface. J Biol Chem. 2009 Jun 5; 284(23):15607-18.
    View in: PubMed
  12. Wang L, Brauner JW, Mao G, Crouch E, Seaton B, Head J, Smith K, Flach CR, Mendelsohn R. Interaction of recombinant surfactant protein D with lipopolysaccharide: conformation and orientation of bound protein by IRRAS and simulations. Biochemistry. 2008 Aug 5; 47(31):8103-13.
    View in: PubMed
  13. Shao C, Novakovic VA, Head JF, Seaton BA, Gilbert GE. Crystal structure of lactadherin C2 domain at 1.7A resolution with mutational and computational analyses of its membrane-binding motif. J Biol Chem. 2008 Mar 14; 283(11):7230-41.
    View in: PubMed
  14. Wang H, Head J, Kosma P, Brade H, Müller-Loennies S, Sheikh S, McDonald B, Smith K, Cafarella T, Seaton B, Crouch E. Recognition of heptoses and the inner core of bacterial lipopolysaccharides by surfactant protein d. Biochemistry. 2008 Jan 15; 47(2):710-20.
    View in: PubMed
  15. Crouch E, McDonald B, Smith K, Roberts M, Mealy T, Seaton B, Head J. Critical role of Arg/Lys343 in the species-dependent recognition of phosphatidylinositol by pulmonary surfactant protein D. Biochemistry. 2007 May 1; 46(17):5160-9.
    View in: PubMed
  16. Shao C, Shi X, Wehbi H, Zambonelli C, Head JF, Seaton BA, Roberts MF. Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C. J Biol Chem. 2007 Mar 23; 282(12):9228-35.
    View in: PubMed
  17. Shao C, Zhang F, Kemp MM, Linhardt RJ, Waisman DM, Head JF, Seaton BA. Crystallographic analysis of calcium-dependent heparin binding to annexin A2. J Biol Chem. 2006 Oct 20; 281(42):31689-95.
    View in: PubMed
  18. Crouch E, McDonald B, Smith K, Cafarella T, Seaton B, Head J. Contributions of phenylalanine 335 to ligand recognition by human surfactant protein D: ring interactions with SP-D ligands. J Biol Chem. 2006 Jun 30; 281(26):18008-14.
    View in: PubMed
  19. Das S, Van Dellen K, Bulik D, Magnelli P, Cui J, Head J, Robbins PW, Samuelson J. The cyst wall of Entamoeba invadens contains chitosan (deacetylated chitin). Mol Biochem Parasitol. 2006 Jul; 148(1):86-92.
    View in: PubMed
  20. Crouch EC, Smith K, McDonald B, Briner D, Linders B, McDonald J, Holmskov U, Head J, Hartshorn K. Species differences in the carbohydrate binding preferences of surfactant protein D. Am J Respir Cell Mol Biol. 2006 Jul; 35(1):84-94.
    View in: PubMed
  21. Meng J, Vardar D, Wang Y, Guo HC, Head JF, McKnight CJ. High-resolution crystal structures of villin headpiece and mutants with reduced F-actin binding activity. Biochemistry. 2005 Sep 13; 44(36):11963-73.
    View in: PubMed
  22. Namboodiri VM, Akey IV, Schmidt-Zachmann MS, Head JF, Akey CW. The structure and function of Xenopus NO38-core, a histone chaperone in the nucleolus. Structure. 2004 Dec; 12(12):2149-60.
    View in: PubMed
  23. Namboodiri VM, Schmidt-Zachmann MS, Head JF, Akey CW. Purification, crystallization and preliminary X-ray analysis of the N-terminal domain of NO38, a nucleolar protein from Xenopus laevis. Acta Crystallogr D Biol Crystallogr. 2004 Dec; 60(Pt 12 Pt 2):2325-7.
    View in: PubMed
  24. Head JF, Mealy TR, McCormack FX, Seaton BA. Crystal structure of trimeric carbohydrate recognition and neck domains of surfactant protein A. J Biol Chem. 2003 Oct 31; 278(44):43254-60.
    View in: PubMed
  25. Stieglitz KA, Seaton BA, Head JF, Stec B, Roberts MF. Unexpected similarity in regulation between an archaeal inositol monophosphatase/fructose bisphosphatase and chloroplast fructose bisphosphatase. Protein Sci. 2003 Apr; 12(4):760-7.
    View in: PubMed
  26. Namboodiri VM, Dutta S, Akey IV, Head JF, Akey CW. The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding. Structure. 2003 Feb; 11(2):175-86.
    View in: PubMed
  27. Mo Y, Campos B, Mealy TR, Commodore L, Head JF, Dedman JR, Seaton BA. Interfacial basic cluster in annexin V couples phospholipid binding and trimer formation on membrane surfaces. J Biol Chem. 2003 Jan 24; 278(4):2437-43.
    View in: PubMed
  28. Head JF, Swamy N, Ray R. Crystal structure of the complex between actin and human vitamin D-binding protein at 2.5 A resolution. Biochemistry. 2002 Jul 23; 41(29):9015-20.
    View in: PubMed
  29. Swamy N, Head JF, Weitz D, Ray R. Biochemical and preliminary crystallographic characterization of the vitamin D sterol- and actin-binding by human vitamin D-binding protein. Arch Biochem Biophys. 2002 Jun 1; 402(1):14-23.
    View in: PubMed
  30. Stieglitz KA, Johnson KA, Yang H, Roberts MF, Seaton BA, Head JF, Stec B. Crystal structure of a dual activity IMPase/FBPase (AF2372) from Archaeoglobus fulgidus. The story of a mobile loop. J Biol Chem. 2002 Jun 21; 277(25):22863-74.
    View in: PubMed
  31. Dutta S, Akey IV, Dingwall C, Hartman KL, Laue T, Nolte RT, Head JF, Akey CW. The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly. Mol Cell. 2001 Oct; 8(4):841-53.
    View in: PubMed
  32. Alekshun MN, Levy SB, Mealy TR, Seaton BA, Head JF. The crystal structure of MarR, a regulator of multiple antibiotic resistance, at 2.3 A resolution. Nat Struct Biol. 2001 Aug; 8(8):710-4.
    View in: PubMed
  33. Head JF, Inouye S, Teranishi K, Shimomura O. The crystal structure of the photoprotein aequorin at 2.3 A resolution. Nature. 2000 May 18; 405(6784):372-6.
    View in: PubMed
  34. Campos B, Mo YD, Mealy TR, Li CW, Swairjo MA, Balch C, Head JF, Retzinger G, Dedman JR, Seaton BA. Mutational and crystallographic analyses of interfacial residues in annexin V suggest direct interactions with phospholipid membrane components. Biochemistry. 1998 Jun 2; 37(22):8004-10.
    View in: PubMed
  35. Jin L, Seaton BA, Head JF. Crystal structure at 2.8 A resolution of anabolic ornithine transcarbamylase from Escherichia coli. Nat Struct Biol. 1997 Aug; 4(8):622-5.
    View in: PubMed
  36. Seaton BA, Head JF. Shaking hands with yourself. Nat Struct Biol. 1994 Jun; 1(6):350-1.
    View in: PubMed
  37. Head JF. Phototransduction. Shedding light on recoverin. Curr Biol. 1994 Jan 1; 4(1):64-6.
    View in: PubMed
  38. Concha NO, Head JF, Kaetzel MA, Dedman JR, Seaton BA. Rat annexin V crystal structure: Ca(2+)-induced conformational changes. Science. 1993 Sep 3; 261(5126):1321-4.
    View in: PubMed
  39. Concha NO, Head JF, Kaetzel MA, Dedman JR, Seaton BA. Annexin V forms calcium-dependent trimeric units on phospholipid vesicles. FEBS Lett. 1992 Dec 14; 314(2):159-62.
    View in: PubMed
  40. Head JF. A better grip on calmodulin. Curr Biol. 1992 Nov; 2(11):609-11.
    View in: PubMed
  41. Kataoka M, Head JF, Vorherr T, Krebs J, Carafoli E. Small-angle X-ray scattering study of calmodulin bound to two peptides corresponding to parts of the calmodulin-binding domain of the plasma membrane Ca2+ pump. Biochemistry. 1991 Jun 25; 30(25):6247-51.
    View in: PubMed
  42. Kataoka M, Head JF, Persechini A, Kretsinger RH, Engelman DM. Small-angle X-ray scattering studies of calmodulin mutants with deletions in the linker region of the central helix indicate that the linker region retains a predominantly alpha-helical conformation. Biochemistry. 1991 Feb 5; 30(5):1188-92.
    View in: PubMed
  43. Seaton BA, Head JF, Kaetzel MA, Dedman JR. Purification, crystallization, and preliminary X-ray diffraction analysis of rat kidney annexin V, a calcium-dependent phospholipid-binding protein. J Biol Chem. 1990 Mar 15; 265(8):4567-9.
    View in: PubMed
  44. Kataoka M, Head JF, Seaton BA, Engelman DM. Melittin binding causes a large calcium-dependent conformational change in calmodulin. Proc Natl Acad Sci U S A. 1989 Sep; 86(18):6944-8.
    View in: PubMed
  45. Head JF. Amino acid sequence of a low molecular weight, high affinity calcium-binding protein from the optic lobe of the squid Loligo pealei. J Biol Chem. 1989 May 5; 264(13):7202-9.
    View in: PubMed
  46. Sheldon A, Head JF. Calcium-binding properties of two high affinity calcium-binding proteins from squid optic lobe. J Biol Chem. 1988 Oct 5; 263(28):14384-9.
    View in: PubMed
  47. Oberdorf JA, Lebeche D, Head JF, Kaminer B. Identification of a calsequestrin-like protein from sea urchin eggs. J Biol Chem. 1988 May 15; 263(14):6806-9.
    View in: PubMed
  48. Oberdorf JA, Head JF, Kaminer B. Calcium uptake and release by isolated cortices and microsomes from the unfertilized egg of the sea urchin Strongylocentrotus droebachiensis. J Cell Biol. 1986 Jun; 102(6):2205-10.
    View in: PubMed
  49. Seaton BA, Head JF, Engelman DM, Richards FM. Calcium-induced increase in the radius of gyration and maximum dimension of calmodulin measured by small-angle X-ray scattering. Biochemistry. 1985 Nov 19; 24(24):6740-3.
    View in: PubMed
  50. Gopalakrishna R, Head JF. Rapid purification of calcium-activated protease by calcium-dependent hydrophobic-interaction chromatography. FEBS Lett. 1985 Jul 8; 186(2):246-50.
    View in: PubMed
  51. Masure HR, Head JF, Tice HM. Studies on the alpha-subunit of bovine brain S-100 protein. Biochem J. 1984 Mar 15; 218(3):691-6.
    View in: PubMed
  52. Head JF, Spielberg S, Kaminer B. Two low-molecular-weight Ca2+-binding proteins isolated from squid optic lobe by phenothiazine--Sepharose affinity chromatography. Biochem J. 1983 Mar 1; 209(3):797-802.
    View in: PubMed
  53. Seaton BA, Head JF, Lord RC, Petsko GA. Studies of calmodulin structure: laser raman spectroscopy of biomolecules. Biochemistry. 1983 Feb 15; 22(4):973-8.
    View in: PubMed
  54. Stommel EW, Stephens RE, Masure HR, Head JF. Specific localization of scallop gill epithelial calmodulin in cilia. J Cell Biol. 1982 Mar; 92(3):622-8.
    View in: PubMed
  55. Head JF, Masure HR, Kaminer B. Identification and purification of a phenothiazine binding fragment from bovine brain calmodulin. FEBS Lett. 1982 Jan 11; 137(1):71-4.
    View in: PubMed
  56. Lehman W, Head JF, Grant PW. The stoichiometry and location of troponin I- and troponin C-like proteins in the myofibril of the bay scallop, Aequipecten irradians. Biochem J. 1980 May 1; 187(2):447-56.
    View in: PubMed
  57. Head JF, Birnbaum RJ, Kaminer B. Calmodulin-activated phosphodiesterase from vertebrate smooth muscle. Ann N Y Acad Sci. 1980; 356:33-5.
    View in: PubMed
  58. Head JF, Mader S, Kaminer B. Calcium-binding modulator protein from the unfertilized egg of the sea urchin Arbacia punctulata. J Cell Biol. 1979 Jan; 80(1):211-8.
    View in: PubMed
  59. Head JF, Perry SV. The interaction of the calcium-binding protein (troponin C) with bivalent cations and the inhibitory protein (troponin I). Biochem J. 1974 Feb; 137(2):145-54.
    View in: PubMed
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