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Last Name

Brigitte Ritter, PhD

TitleAssistant Professor
InstitutionBoston University School of Medicine
DepartmentBiochemistry
Address72 E. Concord St Silvio Conte (K)
Boston MA 02118
Phone(617) 638-5064
ORCID ORCID Icon0000-0002-0094-0460
 Research Expertise & Professional Interests
Intracellular receptor sorting controls basic cellular functions such as nutrient uptake and signaling as well as complex behaviors such as migration, differentiation and tissue morphogenesis. Accordingly, the proteins and mechanisms involved in the various sorting pathways are key to normal cell physiology and their misregulation directly contributes to human disease.

The focus of our lab is on clathrin-mediated sorting events and the regulation of these events by the NECAP protein family. NECAP1 controls the composition of the endocytic clathrin machinery to ensure normal vesicle formation and receptor internalization at the plasma membrane. In addition, NECAP1 is critical for synaptic vesicle recycling in primary neurons and a loss-of-function mutation in NECAP1 (EIEE21) has recently been identified as a heritable genetic cause of Ohtahara Syndrome/Early Infantile Epileptic Encephalopathy (EIEE). NECAP2 is not involved in clathrin-mediated endocytosis but instead controls the recycling of receptors from early endosomes to the cell surface. Since endosomal sorting pathways are still some of the least understood, we are now using NECAP2 to define the proteins and mechanisms involved in the regulation of receptor recycling and to understand the importance of this pathway for cell function.

 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Chamberland JP, Antonow LT, Dias Santos M, Ritter B. NECAP2 controls clathrin coat recruitment to early endosomes for fast endocytic recycling. J Cell Sci. 2016 Jul 01; 129(13):2625-37. PMID: 27206861.
    View in: PubMed
  2. Rouka E, Simister PC, Janning M, Kumbrink J, Konstantinou T, Muniz JR, Joshi D, O'Reilly N, Volkmer R, Ritter B, Knapp S, von Delft F, Kirsch KH, Feller SM. Differential Recognition Preferences of the Three Src Homology 3 (SH3) Domains from the Adaptor CD2-associated Protein (CD2AP) and Direct Association with Ras and Rab Interactor 3 (RIN3). J Biol Chem. 2015 Oct 16; 290(42):25275-92. PMID: 26296892.
    View in: PubMed
  3. Allaire PD, McPherson PS, Ritter B. Analysis of connecdenn 1-3 (DENN1A-C) GEF activity for Rab35. Methods Mol Biol. 2015; 1298:217-31. PMID: 25800846.
    View in: PubMed
  4. Wu Y, O'Toole ET, Girard M, Ritter B, Messa M, Liu X, McPherson PS, Ferguson SM, De Camilli P. A dynamin 1-, dynamin 3- and clathrin-independent pathway of synaptic vesicle recycling mediated by bulk endocytosis. Elife. 2014; 3:e01621. PMID: 24963135.
    View in: PubMed
  5. Ritter B, Murphy S, Dokainish H, Girard M, Gudheti MV, Kozlov G, Halin M, Philie J, Jorgensen EM, Gehring K, McPherson PS. NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis. PLoS Biol. 2013 Oct; 11(10):e1001670. PMID: 24130457.
    View in: PubMed
  6. Allaire PD, Seyed Sadr M, Chaineau M, Seyed Sadr E, Konefal S, Fotouhi M, Maret D, Ritter B, Del Maestro RF, McPherson PS. Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration. J Cell Sci. 2013 Feb 1; 126(Pt 3):722-31. PMID: 23264734.
    View in: PubMed
  7. Kent CB, Shimada T, Ferraro GB, Ritter B, Yam PT, McPherson PS, Charron F, Kennedy TE, Fournier AE. 14-3-3 proteins regulate protein kinase a activity to modulate growth cone turning responses. J Neurosci. 2010 Oct 20; 30(42):14059-67. PMID: 20962227.
    View in: PubMed
  8. Ceni C, Kommaddi RP, Thomas R, Vereker E, Liu X, McPherson PS, Ritter B, Barker PA. The p75NTR intracellular domain generated by neurotrophin-induced receptor cleavage potentiates Trk signaling. J Cell Sci. 2010 Jul 1; 123(Pt 13):2299-307. PMID: 20530577.
    View in: PubMed
  9. Allaire PD, Marat AL, Dall'Armi C, Di Paolo G, McPherson PS, Ritter B. The Connecdenn DENN domain: a GEF for Rab35 mediating cargo-specific exit from early endosomes. Mol Cell. 2010 Feb 12; 37(3):370-82. PMID: 20159556.
    View in: PubMed
  10. Katoh Y, Ritter B, Gaffry T, Blondeau F, Höning S, McPherson PS. The clavesin family, neuron-specific lipid- and clathrin-binding Sec14 proteins regulating lysosomal morphology. J Biol Chem. 2009 Oct 2; 284(40):27646-54. PMID: 19651769.
    View in: PubMed
  11. Thomas S, Ritter B, Verbich D, Sanson C, Bourbonnière L, McKinney RA, McPherson PS. Intersectin regulates dendritic spine development and somatodendritic endocytosis but not synaptic vesicle recycling in hippocampal neurons. J Biol Chem. 2009 May 1; 284(18):12410-9. PMID: 19258322.
    View in: PubMed
  12. Goodman OB, Barwe SP, Ritter B, McPherson PS, Vasko AJ, Keen JH, Nanus DM, Bander NH, Rajasekaran AK. Interaction of prostate specific membrane antigen with clathrin and the adaptor protein complex-2. Int J Oncol. 2007 Nov; 31(5):1199-203. PMID: 17912448.
    View in: PubMed
  13. Ritter B, Denisov AY, Philie J, Allaire PD, Legendre-Guillemin V, Zylbergold P, Gehring K, McPherson PS. The NECAP PHear domain increases clathrin accessory protein binding potential. EMBO J. 2007 Sep 19; 26(18):4066-77. PMID: 17762867.
    View in: PubMed
  14. Allaire PD, Ritter B, Thomas S, Burman JL, Denisov AY, Legendre-Guillemin V, Harper SQ, Davidson BL, Gehring K, McPherson PS. Connecdenn, a novel DENN domain-containing protein of neuronal clathrin-coated vesicles functioning in synaptic vesicle endocytosis. J Neurosci. 2006 Dec 20; 26(51):13202-12. PMID: 17182770.
    View in: PubMed
  15. Ritter B, McPherson PS. There's a GAP in the ENTH domain. Proc Natl Acad Sci U S A. 2006 Mar 14; 103(11):3953-4. PMID: 16537466.
    View in: PubMed
  16. McPherson PS, Ritter B. Peptide motifs: building the clathrin machinery. Mol Neurobiol. 2005 Aug; 32(1):73-87. PMID: 16077185.
    View in: PubMed
  17. Burman JL, Wasiak S, Ritter B, de Heuvel E, McPherson PS. Aftiphilin is a component of the clathrin machinery in neurons. FEBS Lett. 2005 Apr 11; 579(10):2177-84. PMID: 15811338.
    View in: PubMed
  18. Ritter B, Blondeau F, Denisov AY, Gehring K, McPherson PS. Molecular mechanisms in clathrin-mediated membrane budding revealed through subcellular proteomics. Biochem Soc Trans. 2004 Nov; 32(Pt 5):769-73. PMID: 15494011.
    View in: PubMed
  19. Ritter B, Denisov AY, Philie J, Deprez C, Tung EC, Gehring K, McPherson PS. Two WXXF-based motifs in NECAPs define the specificity of accessory protein binding to AP-1 and AP-2. EMBO J. 2004 Oct 1; 23(19):3701-10. PMID: 15359277.
    View in: PubMed
  20. Denisov AY, Ritter B, McPherson PS, Gehring K. 1H, 15N and 13C resonance assignments and 15N-1H residual dipolar couplings for the alpha-adaptin ear-domain. J Biomol NMR. 2004 Jul; 29(3):441-2. PMID: 15213459.
    View in: PubMed
  21. Blondeau F, Ritter B, Allaire PD, Wasiak S, Girard M, Hussain NK, Angers A, Legendre-Guillemin V, Roy L, Boismenu D, Kearney RE, Bell AW, Bergeron JJ, McPherson PS. Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling. Proc Natl Acad Sci U S A. 2004 Mar 16; 101(11):3833-8. PMID: 15007177.
    View in: PubMed
  22. Mattera R, Ritter B, Sidhu SS, McPherson PS, Bonifacino JS. Definition of the consensus motif recognized by gamma-adaptin ear domains. J Biol Chem. 2004 Feb 27; 279(9):8018-28. PMID: 14665628.
    View in: PubMed
  23. Ritter B, Philie J, Girard M, Tung EC, Blondeau F, McPherson PS. Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif. EMBO Rep. 2003 Nov; 4(11):1089-95. PMID: 14555962.
    View in: PubMed
  24. Modregger J, Schmidt AA, Ritter B, Huttner WB, Plomann M. Characterization of Endophilin B1b, a brain-specific membrane-associated lysophosphatidic acid acyl transferase with properties distinct from endophilin A1. J Biol Chem. 2003 Feb 7; 278(6):4160-7. PMID: 12456676.
    View in: PubMed
  25. Wasiak S, Quinn CC, Ritter B, de Heuvel E, Baranes D, Plomann M, McPherson PS. The Ras/Rac guanine nucleotide exchange factor mammalian Son-of-sevenless interacts with PACSIN 1/syndapin I, a regulator of endocytosis and the actin cytoskeleton. J Biol Chem. 2001 Jul 13; 276(28):26622-8. PMID: 11352907.
    View in: PubMed
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