Keywords
Last Name

Sajal K. Ghosh, PhD

TitleCompliance Coordinator
InstitutionBoston University
DepartmentResearch Compliance
Address704 Commonwealth Ave
Boston MA 02215
Phone(617) 638-4531
ORCID ORCID Icon0000-0002-1187-6994
 Research Expertise & Professional Interests
My broad research interest is to study the role of non-coding RNAs in tumorigenesis.
Project 1.
Over the last decade microRNAs have been identified as master regulator of almost all major biochemical pathways that regulate cellular metabolism. Most importantly, their deregulation is closely related to many cancers. In pancreatic cancer, which has the lowest 5-year survival rate, the level of microRNA let-7 is consistently downregulated. We are interested to identify the link between let-7 downregulation and aggressive growth characteristics of pancreatic cancer cells. We identified that let-7 level is inversely proportional with STAT3 signaling in pancreatic cancer. We are working on the detail mechanism of the process and testing in xenograft model whether let-7 reexpression can be a therapeutic option in pancreatic cancer.

Project 2.
My other research interest is to understand the molecular basis of tumorigenesis by leukemia virus and Epstein Barr virus (EBV). Leukemia viruses are oncogene-deficient simple retroviruses but how their infection in animals causes tumor development in a predicable manner still remains poorly understood. We have demonstrated, using feline leukemia virus as a model, that the long-terminal region (LTR) of these viruses independently activates AP-1 and NF?B signaling pathways in the host cells. We hypothesized that such activities may aid in tumorigenesis. We found that the U3-LTR region makes small non-coding RNA, whose production is directly related to the transactivation potential of the LTR. Our recent studies demonstrated that toll-like receptor 3 (TLR3) expression is necessary for such activity. Our current research effort is dedicated to understand the regulation of expression of the LTR-transcript and how it activates TLR3 signaling.
EBV, on the other hand, is a large DNA tumor virus, associated with several human malignancies. However, EBV maintains a latent state of infection in these cancers and it is believed that the latent gene products play a discrete role in the genesis or maintenance of EBV-associated tumors. Our interest is to determine the contribution of EBV latent gene products in tumorigenesis.

Other Projects:
Additionally in collaborative studies we are also interested in indentifying better therapeutic options against EBV-associated tumors. Prognosis of certain type of lymphomas is very poor when EBV is present in them (such as post-transplantation lymphoproliferative disease, Burkitt’s lymphoma, Hodgkin’s disease). A combination approach where lytic EBV replication is first induced by chemicals and then followed by treatment with anti-herpesvirus drugs, has been previously found to be effective in selective killing of EBV-containing tumor cells. We have and continues to characterize novel histone deacetylase inhibitors or other cytotoxic compounds as inducer of lytic replication of EBV and their usefulness in selective killing of EBV-associated tumors in the combinational therapy model. We are validating efficacy of such approach in xenograft tumor model in NOD/SCID mice.

In another collaborative study we are working on the molecular mechanism of anticancer effect of novel chemically modified plumbagin derivative. Especially, we are interested in the autophagic activity of the compound and its relationship to cell death signaling.


 Self-Described Keywords
  • EBV
  • Gene transactivation
  • Leukemia virus
  • microRNA
  • non-coding RNA
  • Pancreatic Cancer
  • TLR-signaling
 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Patel K, Kollory A, Takashima A, Sarkar S, Faller DV, Ghosh SK. MicroRNA let-7 downregulates STAT3 phosphorylation in pancreatic cancer cells by increasing SOCS3 expression. Cancer Lett. 2014 May 28; 347(1):54-64. PMID: 24491408.
    View in: PubMed
  2. Ghosh SK, Patton JR, Spanjaard RA. A small RNA derived from RNA coactivator SRA blocks steroid receptor signaling via inhibition of Pus1p-mediated pseudouridylation of SRA: evidence of a novel RNA binding domain in the N-terminus of steroid receptors. Biochemistry. 2012 Oct 16; 51(41):8163-72. PMID: 22998747.
    View in: PubMed
  3. Ghosh SK, Perrine SP, Faller DV. Advances in Virus-Directed Therapeutics against Epstein-Barr Virus-Associated Malignancies. Adv Virol. 2012; 2012:509296. PMID: 22500168.
    View in: PubMed
  4. Ghosh SK, Perrine SP, Williams RM, Faller DV. Histone deacetylase inhibitors are potent inducers of gene expression in latent EBV and sensitize lymphoma cells to nucleoside antiviral agents. Blood. 2012 Jan 26; 119(4):1008-17. PMID: 22160379.
    View in: PubMed
  5. Forman LW, Pal-Ghosh R, Spanjaard RA, Faller DV, Ghosh SK. Identification of LTR-specific small non-coding RNA in FeLV infected cells. FEBS Lett. 2009 Apr 17; 583(8):1386-90. PMID: 19336234.
    View in: PubMed
  6. Zhao X, Patton JR, Ghosh SK, Fischel-Ghodsian N, Shen L, Spanjaard RA. Pus3p- and Pus1p-dependent pseudouridylation of steroid receptor RNA activator controls a functional switch that regulates nuclear receptor signaling. Mol Endocrinol. 2007 Mar; 21(3):686-99. PMID: 17170069.
    View in: PubMed
  7. Ghosh SK, Forman LW, Akinsheye I, Perrine SP, Faller DV. Short, discontinuous exposure to butyrate effectively sensitizes latently EBV-infected lymphoma cells to nucleoside analogue antiviral agents. Blood Cells Mol Dis. 2007 Jan-Feb; 38(1):57-65. PMID: 17161633.
    View in: PubMed
  8. Abujamra AL, Spanjaard RA, Akinsheye I, Zhao X, Faller DV, Ghosh SK. Leukemia virus long terminal repeat activates NFkappaB pathway by a TLR3-dependent mechanism. Virology. 2006 Feb 20; 345(2):390-403. PMID: 16289658.
    View in: PubMed
  9. Abujamra AL, Faller DV, Ghosh SK. Mutations that abrogate transactivational activity of the feline leukemia virus long terminal repeat do not affect virus replication. Virology. 2003 May 10; 309(2):294-305. PMID: 12758176.
    View in: PubMed
  10. Ghosh SK, Roy-Burman P, Faller DV. Long terminal repeat regions from exogenous but not endogenous feline leukemia viruses transactivate cellular gene expression. J Virol. 2000 Oct; 74(20):9742-8. PMID: 11000248.
    View in: PubMed
  11. Ghosh SK, Faller DV. Feline leukemia virus long terminal repeat activates collagenase IV gene expression through AP-1. J Virol. 1999 Jun; 73(6):4931-40. PMID: 10233955.
    View in: PubMed
  12. Ohagen A, Ghosh S, He J, Huang K, Chen Y, Yuan M, Osathanondh R, Gartner S, Shi B, Shaw G, Gabuzda D. Apoptosis induced by infection of primary brain cultures with diverse human immunodeficiency virus type 1 isolates: evidence for a role of the envelope. J Virol. 1999 Feb; 73(2):897-906. PMID: 9882290.
    View in: PubMed
  13. Ratner L, Joseph T, Bandres J, Ghosh S, Vander Heyden N, Templeton A, Hahn B, Powderly W, Arens M. Sequence heterogeneity of Nef transcripts in HIV-1-infected subjects at different stages of disease. Virology. 1996 Sep 1; 223(1):245-50. PMID: 8806559.
    View in: PubMed
  14. Wei X, Ghosh SK, Taylor ME, Johnson VA, Emini EA, Deutsch P, Lifson JD, Bonhoeffer S, Nowak MA, Hahn BH, et al. Viral dynamics in human immunodeficiency virus type 1 infection. Nature. 1995 Jan 12; 373(6510):117-22. PMID: 7529365.
    View in: PubMed
  15. Benos DJ, Hahn BH, Bubien JK, Ghosh SK, Mashburn NA, Chaikin MA, Shaw GM, Benveniste EN. Envelope glycoprotein gp120 of human immunodeficiency virus type 1 alters ion transport in astrocytes: implications for AIDS dementia complex. Proc Natl Acad Sci U S A. 1994 Jan 18; 91(2):494-8. PMID: 8290553.
    View in: PubMed
  16. Curiel TJ, Cook DR, Wang Y, Hahn BH, Ghosh SK, Harrison GS. Long-term inhibition of clinical and laboratory human immunodeficiency virus strains in human T-cell lines containing an HIV-regulated diphtheria toxin A chain gene. Hum Gene Ther. 1993 Dec; 4(6):741-7. PMID: 8186289.
    View in: PubMed
  17. Ghosh SK, Fultz PN, Keddie E, Saag MS, Sharp PM, Hahn BH, Shaw GM. A molecular clone of HIV-1 tropic and cytopathic for human and chimpanzee lymphocytes. Virology. 1993 Jun; 194(2):858-64. PMID: 8503191.
    View in: PubMed
  18. Ghosh SK, Naik TN. Evidence for a new rotavirus subgroup in India. Epidemiol Infect. 1989 Jun; 102(3):523-30. PMID: 2544441.
    View in: PubMed
  19. Ghosh SK, Naik TN. Detection of a large number of subgroup 1 human rotaviruses with a "long" RNA electropherotype. Arch Virol. 1989; 105(1-2):119-27. PMID: 2541674.
    View in: PubMed
  20. Palchaudhuri, S., Kumar, R., Sen, D., Pal, R., Ghosh, S., Sarkar, B. L., Bhattacharya, S. and Pal, S. C. Molecular epidemiology of plasmid patterns of Shigella dysenteriae type I from an outbreak in West Bengal (India). FEMS Microbiology Letters. 1985; 30:189-195.
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