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Last Name

John Caradonna, PhD

TitleAssociate Professor
InstitutionBoston University College of Arts and Sciences
DepartmentChemistry
Address24 Cummington Mall
Boston MA 02215
Phone(617) 353-1692
Fax(617) 353-6466
Other Positions
TitleResearch Associate Professor
InstitutionBoston University School of Medicine
DepartmentPharmacology & Experimental Therapeutics

 Research Expertise & Professional Interests
The Caradonna research group is interested in understanding the mechanism of action of non-heme iron metalloproteins, with a focus on the chemistry of metalloenzyme active sites involved in biological oxidation reactions. Our efforts span the continuum from the investigation of small molecule synthetic complexes that catalyze oxygen atom transfer reactions, to the characterization of an interesting class of iron/pterin-dependent aromatic amino acid hydroxylases (phenylalanine, tyrosine, and tryptophan hydroxylases), to the computational design of functional metalloproteins (iron-sulfur-based electron transfer clusters, Fe and Mn superoxide dismutanse). A broad-based approach, including chemical, molecular biological, and biophysical (spectroscopic) techniques, is used to develop a global understanding of the intrinsic characteristics of the active site metal centers and the role played by the protein matrix in regulating, modulating, and tuning these properties.

 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. McCracken J, Cappillino PJ, McNally JS, Krzyaniak MD, Howart M, Tarves PC, Caradonna JP. Characterization of Water Coordination to Ferrous Nitrosyl Complexes with fac-N2O, cis-N2O2, and N2O3 Donor Ligands. Inorg Chem. 2015 Jul 6; 54(13):6486-97. PMID: 26090963.
    View in: PubMed
  2. Cappillino PJ, Miecznikowski JR, Tyler LA, Tarves PC, McNally JS, Lo W, Kasibhatla BS, Krzyaniak MD, McCracken J, Wang F, Armstrong WH, Caradonna JP. Studies of iron(II) and iron(III) complexes with fac-N2O, cis-N2O2 and N2O3 donor ligands: models for the 2-His 1-carboxylate motif of non-heme iron monooxygenases. Dalton Trans. 2012 May 14; 41(18):5662-77. PMID: 22434362.
    View in: PubMed
  3. Gregor LC, Rowe GT, Rybak-Akimova E, Caradonna JP. Mechanistic investigation of cyclohexane oxidation by a non-heme iron complex: evidence of product inhibition by UV/vis stopped-flow studies. Dalton Trans. 2012 Jan 21; 41(3):777-82. PMID: 22109726.
    View in: PubMed
  4. Cappillino PJ, McNally JS, Wang F, Caradonna JP. The effect of varying carboxylate ligation on the electronic environment of N2O(x) (x = 1-3) nonheme iron: a DFT analysis. Dalton Trans. 2012 Jan 14; 41(2):474-83. PMID: 22042235.
    View in: PubMed
  5. Cong H, Ledbetter D, Rowe GT, Caradonna JP, Porco JA. Electron transfer-initiated Diels-Alder cycloadditions of 2'-hydroxychalcones. J Am Chem Soc. 2008 Jul 23; 130(29):9214-5. PMID: 18576647.
    View in: PubMed
  6. Rowe GT, Rybak-Akimova EV, Caradonna JP. Heterolytic cleavage of peroxide by a diferrous compound generates metal-based intermediates identical to those observed with reactions utilizing oxygen-atom-donor molecules. Chemistry. 2008; 14(27):8303-11. PMID: 18680115.
    View in: PubMed
  7. Rowe GT, Rybak-Akimova EV, Caradonna JP. Unraveling the reactive species of a functional non-heme iron monooxygenase model using stopped-flow UV-vis spectroscopy. Inorg Chem. 2007 Dec 10; 46(25):10594-606. PMID: 17988120.
    View in: PubMed
  8. Kemsley JN, Wasinger EC, Datta S, Mitic N, Acharya T, Hedman B, Caradonna JP, Hodgson KO, Solomon EI. Spectroscopic and kinetic studies of PKU-inducing mutants of phenylalanine hydroxylase: Arg158Gln and Glu280Lys. J Am Chem Soc. 2003 May 14; 125(19):5677-86. PMID: 12733906.
    View in: PubMed
  9. Foster TL, Caradonna JP. Fe2+-catalyzed heterolytic RO-OH bond cleavage and substrate oxidation: a functional synthetic non-heme iron monooxygenase system. J Am Chem Soc. 2003 Apr 2; 125(13):3678-9. PMID: 12656580.
    View in: PubMed
  10. Wasinger EC, Mitic N, Hedman B, Caradonna J, Solomon EI, Hodgson KO. X-ray absorption spectroscopic investigation of the resting ferrous and cosubstrate-bound active sites of phenylalanine hydroxylase. Biochemistry. 2002 May 21; 41(20):6211-7. PMID: 12009881.
    View in: PubMed
  11. Coldren CD, Hellinga HW, Caradonna JP. The rational design and construction of a cuboidal iron-sulfur protein. Proc Natl Acad Sci U S A. 1997 Jun 24; 94(13):6635-40. PMID: 9192617.
    View in: PubMed
  12. Pinto AL, Hellinga HW, Caradonna JP. Construction of a catalytically active iron superoxide dismutase by rational protein design. Proc Natl Acad Sci U S A. 1997 May 27; 94(11):5562-7. PMID: 9159112.
    View in: PubMed
  13. Narayan VA, Kriwacki RW, Caradonna JP. Structures of zinc finger domains from transcription factor Sp1. Insights into sequence-specific protein-DNA recognition. J Biol Chem. 1997 Mar 21; 272(12):7801-9. PMID: 9065444.
    View in: PubMed
  14. Kappock TJ, Caradonna JP. Pterin-Dependent Amino Acid Hydroxylases. Chem Rev. 1996 Nov 07; 96(7):2659-2756. PMID: 11848840.
    View in: PubMed
  15. Kappock TJ, Harkins PC, Friedenberg S, Caradonna JP. Spectroscopic and kinetic properties of unphosphorylated rat hepatic phenylalanine hydroxylase expressed in Escherichia coli. Comparison of resting and activated states. J Biol Chem. 1995 Dec 22; 270(51):30532-44. PMID: 8530485.
    View in: PubMed
  16. Caradonna JP, Stassinopoulos A. Functional non-heme iron metalloenzyme model systems. Adv Inorg Biochem. 1994; 9:245-315. PMID: 8140949.
    View in: PubMed
  17. Kriwacki RW, Schultz SC, Steitz TA, Caradonna JP. Sequence-specific recognition of DNA by zinc-finger peptides derived from the transcription factor Sp1. Proc Natl Acad Sci U S A. 1992 Oct 15; 89(20):9759-63. PMID: 1329106.
    View in: PubMed
  18. Hellinga HW, Caradonna JP, Richards FM. Construction of new ligand binding sites in proteins of known structure. II. Grafting of a buried transition metal binding site into Escherichia coli thioredoxin. J Mol Biol. 1991 Dec 5; 222(3):787-803. PMID: 1660933.
    View in: PubMed
  19. Caradonna JP, Harlan EW, Holm RH. Thiol as an electron donor in molybdenum oxo-transferase analog reaction systems: observations by fluorine-19 NMR spectroscopy and biological implications. J Am Chem Soc. 1986 Nov 1; 108(24):7856-8. PMID: 22283311.
    View in: PubMed
  20. Ushay HM, Santella RM, Caradonna JP, Grunberger D, Lippard SJ. Binding of [(dien)PtCl] Cl to poly(dG-dC)-poly(dG-dC) facilitates the B goes to Z conformational transition. Nucleic Acids Res. 1982 Jun 11; 10(11):3573-88. PMID: 7201636.
    View in: PubMed
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