Daniel Dempsey | Profiles RNS

Daniel Dempsey, PhD

Assistant Professor

Boston University Chobanian & Avedisian School of Medicine

Dermatology

PhD, University of South Florida
MS, State University of New York at Oswego
BA, State University of New York at Oswego

Websites

	Boston University School of Medicine: Dermatology
	ResearchGate

ORCID : 0000-0001-6104-0805

The mission of Prof. Dempsey's lab is to understand the basic biological mechanisms of proteins that are essential to human health and disease. The lab focuses on investigating the structure, function, and regulation of proteins involved in cell signaling, epigenetics, and gene regulation. Prof. Dempsey and his team use chemical, semi-synthetic, biophysical, and cellular approaches to elucidate mechanisms for how post-translational modifications regulate protein function. They are also interested in advancing new chemical strategies to investigate PTMs and protein function.

Prof. Dempsey's current focus is on understanding the function and regulation of deubiquitinases and RNA-modifying proteins, which are emerging as attractive therapeutic targets in several cancers. Their work will shed light on the mechanistic consequence of PTMs, and how dysregulation of these proteins may lead to disease. The team's goal is that these findings will pave the way for the rational design of new therapies for the treatment of cancer.

Assistant Professor
Boston University Chobanian & Avedisian School of Medicine
Pharmacology, Physiology & Biophysics

BU Research

Molecular Mechanisms of PTEN and USP7 Regulation
03/01/2022 - 02/28/2025 (PI)
NIH/National Institute of General Medical Sciences
5R00GM130961-05

Title

Yr	Title	Project-Sub Proj	Pubs

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis Copy PMIDs To Clipboard

Iwase R, Dempsey DR, Whedon SD, Jiang H, Palanski BA, Deng B, Cole PA. Semisynthetic Approach to the Analysis of Tumor Suppressor PTEN Ubiquitination. J Am Chem Soc. 2023 Mar 22; 145(11):6039-6044. PMID: 36897111; PMCID: PMC10071500; DOI: 10.1021/jacs.2c13871;

Viennet T, Rodriguez Ospina S, Lu Y, Cui A, Arthanari H, Dempsey DR. Chemical and structural approaches to investigate PTEN function and regulation. Methods Enzymol. 2023; 682:289-318.View Related Profiles.

Dempsey, Daniel
Rodriguez Ospina, Santiago

PMID: 36948705; PMCID: PMC10037535; DOI: 10.1016/bs.mie.2022.09.007;

Dempsey, Daniel
Rodriguez Ospina, Santiago

PMID: 36948705; PMCID: PMC10037535; DOI: 10.1016/bs.mie.2022.09.007;

Dempsey DR, Viennet T, Iwase R, Park E, Henriquez S, Chen Z, Jeliazkov JR, Palanski BA, Phan KL, Coote P, Gray JJ, Eck MJ, Gabelli SB, Arthanari H, Cole PA. The structural basis of PTEN regulation by multi-site phosphorylation. Nat Struct Mol Biol. 2021 10; 28(10):858-868. PMID: 34625746; PMCID: PMC8549118; DOI: 10.1038/s41594-021-00668-5;

Jiang H, Dempsey DR, Cole PA. Ubiquitin Ligase Activities of WWP1 Germline Variants K740N and N745S. Biochemistry. 2021 02 09; 60(5):357-364. PMID: 33470109; PMCID: PMC8262107; DOI: 10.1021/acs.biochem.0c00869;

Jiang H, D'Agostino GD, Cole PA, Dempsey DR. Selective protein N-terminal labeling with N-hydroxysuccinimide esters. Methods Enzymol. 2020; 639:333-353. PMID: 32475408; PMCID: PMC7340118; DOI: 10.1016/bs.mie.2020.04.018;

Henager SH, Henriquez S, Dempsey DR, Cole PA. Analysis of Site-Specific Phosphorylation of PTEN by Using Enzyme-Catalyzed Expressed Protein Ligation. Chembiochem. 2020 01 15; 21(1-2):64-68. PMID: 31206229; PMCID: PMC7012368; DOI: 10.1002/cbic.201900316;

Chu N, Salguero AL, Liu AZ, Chen Z, Dempsey DR, Ficarro SB, Alexander WM, Marto JA, Li Y, Amzel LM, Gabelli SB, Cole PA. Akt Kinase Activation Mechanisms Revealed Using Protein Semisynthesis. Cell. 2018 08 09; 174(4):897-907.e14. PMID: 30078705; PMCID: PMC6139374; DOI: 10.1016/j.cell.2018.07.003;

Dempsey DR, Jiang H, Kalin JH, Chen Z, Cole PA. Site-Specific Protein Labeling with N-Hydroxysuccinimide-Esters and the Analysis of Ubiquitin Ligase Mechanisms. J Am Chem Soc. 2018 08 01; 140(30):9374-9378. PMID: 30016585; PMCID: PMC6093203; DOI: 10.1021/jacs.8b05098;

Dempsey DR, Cole PA. Protein Chemical Approaches to Understanding PTEN Lipid Phosphatase Regulation. Methods Enzymol. 2018; 607:405-422. PMID: 30149868; PMCID: PMC6231048; DOI: 10.1016/bs.mie.2018.05.007;

Showing 10 of 30 results. Show More

Handa S, Dempsey DR, Ramamoorthy D, Cook N, Guida WC, Spradling TJ, White JK, Woodcock HL, Merkler DJ. Mechanistic Studies of 1-Deoxy-D-Xylulose-5-Phosphate Synthase from Deinococcus radiodurans. Biochem Mol Biol J. 2018; 4(1). PMID: 29552677; PMCID: PMC5851014; DOI: 10.21767/2471-8084.100051;

Dempsey DR, Nichols DA, Battistini MR, Pemberton O, Ospina SR, Zhang X, Carpenter AM, O'Flynn BG, Leahy JW, Kanwar A, Lewandowski EM, Chen Y, Merkler DJ. Structural and Mechanistic Analysis of Drosophila melanogaster Agmatine N-Acetyltransferase, an Enzyme that Catalyzes the Formation of N-Acetylagmatine. Sci Rep. 2017 10 18; 7(1):13432.View Related Profiles.

Dempsey, Daniel
Rodriguez Ospina, Santiago

PMID: 29044148; PMCID: PMC5647378; DOI: 10.1038/s41598-017-13669-6;

Chen Z, Jiang H, Xu W, Li X, Dempsey DR, Zhang X, Devreotes P, Wolberger C, Amzel LM, Gabelli SB, Cole PA. A Tunable Brake for HECT Ubiquitin Ligases. Mol Cell. 2017 May 04; 66(3):345-357.e6. PMID: 28475870; PMCID: PMC5489419; DOI: 10.1016/j.molcel.2017.03.020;

Henager SH, Chu N, Chen Z, Bolduc D, Dempsey DR, Hwang Y, Wells J, Cole PA. Enzyme-catalyzed expressed protein ligation. Nat Methods. 2016 Nov; 13(11):925-927. PMID: 27669326; PMCID: PMC5088058; DOI: 10.1038/nmeth.4004;

Chen Z, Dempsey DR, Thomas SN, Hayward D, Bolduc DM, Cole PA. Molecular Features of Phosphatase and Tensin Homolog (PTEN) Regulation by C-terminal Phosphorylation. J Biol Chem. 2016 Jul 01; 291(27):14160-14169. PMID: 27226612; PMCID: PMC4933174; DOI: 10.1074/jbc.M116.728980;

Jeffries KA, Dempsey DR, Farrell EK, Anderson RL, Garbade GJ, Gurina TS, Gruhonjic I, Gunderson CA, Merkler DJ. Glycine N-acyltransferase-like 3 is responsible for long-chain N-acylglycine formation in N18TG2 cells. J Lipid Res. 2016 05; 57(5):781-90. PMID: 27016726; PMCID: PMC4847626; DOI: 10.1194/jlr.M062042;

Dempsey DR, Carpenter AM, Ospina SR, Merkler DJ. Probing the chemical mechanism and critical regulatory amino acid residues of Drosophila melanogaster arylalkylamine N-acyltransferase like 2. Insect Biochem Mol Biol. 2015 Nov; 66:1-12.View Related Profiles.

Dempsey, Daniel
Rodriguez Ospina, Santiago

PMID: 26476413; PMCID: PMC4663176; DOI: 10.1016/j.ibmb.2015.10.003;

Taylor MS, Dempsey DR, Hwang Y, Chen Z, Chu N, Boeke JD, Cole PA. Mechanistic analysis of ghrelin-O-acyltransferase using substrate analogs. Bioorg Chem. 2015 Oct; 62:64-73. PMID: 26246082; PMCID: PMC4567917; DOI: 10.1016/j.bioorg.2015.07.003;

Dempsey DR, Jeffries KA, Handa S, Carpenter AM, Rodriguez-Ospina S, Breydo L, Merkler DJ. Mechanistic and Structural Analysis of a Drosophila melanogaster Enzyme, Arylalkylamine N-Acetyltransferase Like 7, an Enzyme That Catalyzes the Formation of N-Acetylarylalkylamides and N-Acetylhistamine. Biochemistry. 2015 Apr 28; 54(16):2644-58. PMID: 25850002; PMCID: PMC4749145; DOI: 10.1021/acs.biochem.5b00113;

Dempsey DR, Jeffries KA, Bond JD, Carpenter AM, Rodriguez-Ospina S, Breydo L, Caswell KK, Merkler DJ. Mechanistic and structural analysis of Drosophila melanogaster arylalkylamine N-acetyltransferases. Biochemistry. 2014 Dec 16; 53(49):7777-93. PMID: 25406072; PMCID: PMC4270386; DOI: 10.1021/bi5006078;

Choudhury RA, Murayama KM, Neylan CJ, Savulionyte G, Glick HA, Williams NN, Dempsey DT, Dumon KR. Re-examining the BMI threshold for bariatric surgery in the USA. J Gastrointest Surg. 2014 Dec; 18(12):2074-9. PMID: 25297444

Jeffries KA, Dempsey DR, Behari AL, Anderson RL, Merkler DJ. Drosophila melanogaster as a model system to study long-chain fatty acid amide metabolism. FEBS Lett. 2014 May 02; 588(9):1596-602. PMID: 24650760; PMCID: PMC4023565; DOI: 10.1016/j.febslet.2014.02.051;

Dempsey DR, Bond JD, Carpenter AM, Rodriguez Ospina S, Merkler DJ. Expression, purification, and characterization of mouse glycine N-acyltransferase in Escherichia coli. Protein Expr Purif. 2014 May; 97:23-8.View Related Profiles.

Dempsey, Daniel
Rodriguez Ospina, Santiago

PMID: 24576660; PMCID: PMC3985065; DOI: 10.1016/j.pep.2014.02.007;

Dempsey DR, Jeffries KA, Anderson RL, Carpenter AM, Rodriquez Opsina S, Merkler DJ. Identification of an arylalkylamine N-acyltransferase from Drosophila melanogaster that catalyzes the formation of long-chain N-acylserotonins. FEBS Lett. 2014 Feb 14; 588(4):594-9. PMID: 24444601

Waluk DP, Battistini MR, Dempsey DR, Farrell EK, Jeffries KA, Mitchell P Jr, Hernandez LW, McBride JC, Merkler DJ, Hunt MC. Omega-3 Fatty Acids in Neurological Health. Mammalian fatty acid amides of the brain and CNS. Academic Press. Waltham, MA. 2014.

Handa S, Spradling TJ, Dempsey DR, Merkler DJ. Production of the catalytic core of human peptidylglycine a-hydroxylating monooxygenase (hPHMcc) in Escherichia coli. Protein Expr Purif. 2012 Jul; 84(1):9-13. PMID: 22554821

Bendinskas K, Sattelberg P, Crossett D, Banyikwa A, Dempsey D, MacKenzie JA. Enzymatic detection of ?-hydroxybutyrate using aldo-keto reductase 7A2. J Forensic Sci. 2011 May; 56(3):783-7. PMID: 21521220; DOI: 10.1111/j.1556-4029.2010.01694.x;

Ivkovic M, Dempsey DR, Handa S, Hilton JH, Lowe EW, Merkler DJ. N-acylethanolamines as novel alcohol dehydrogenase 3 substrates. Arch Biochem Biophys. 2011 Feb 15; 506(2):157-64. PMID: 21144815; PMCID: PMC3030644; DOI: 10.1016/j.abb.2010.12.002;

Gupta D, Macha M, Piacentino V, Singhal AK, Sasken HF, Furukawa S, Dempsey DT. Successful treatment of esophageal cancer with transhiatal esophagectomy after heart transplantation. Ann Thorac Surg. 2004 Aug; 78(2):702-5. PMID: 15276556

Volkov VV, Kolesnikova LN, Shelepin IuE, Gavrishchuk VI, Koroteeva AN, Pavlov NN. [Assessment of individual eye protection devices based on their effect on the frequency-contrast characteristics of the visual analyzer]. Oftalmol Zh. 1990; (3):164-5. PMID: 2255482

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 29 publications over 15 distinct years, with a maximum of 6 publications in 2014

Bar chart showing 29 publications over 15 distinct years, with a maximum of 6 publications in 2014

Year	Publications
1990	1
2004	1
2010	1
2011	1
2012	1
2014	6
2015	3
2016	3
2017	2
2018	4
2019	1
2020	1
2021	2
2022	1
2023	1

Media Mentions

University Names Eight Junior Faculty to Career Development Professorships for Promising Scholars

BU Today 9/26/2022

Dr. Daniel Dempsey Receives Dahod Pilot Grant

Boston University School of Medicine 1/1/2022

2019 Brigham and Women’s Hospital, Boston, MA: Chair’s Research Award

2015 National Institutes of Health: Ruth L. Kirschstein National Research Service Award

2014 University of South Florida, Tampa, FL: Drug Discovery and Infectious Disease Travel Award

2014 University of South Florida, Tampa, FL: Dissertation Completion Fellowship

2014 Steenbock Symposium: Best Poser Prize

2014 International Conference on the Bioscience of Lipids: Travel Grant

2014 International Conference on the Bioscience of Lipids: Young Investigator Award

Boston University School of Medicine: Dahod Pilot Grant

Contact for Mentoring:

670 Albany Street
Boston MA 02118
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317.358.9700

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Rodriguez Ospina, Santiago

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