Anton Vladimirovich Kermanov
Postdoctoral Associate
Boston University Chobanian & Avedisian School of Medicine
Biochemistry & Cell Biology

I worked in different institutions around Europe studying animal biochemistry, human genetics, bacteriology, plant biology and bioinformatics.
I am independent in experiment design and vast of molecular biology techniques, but especially I like RNA biology.
My strong sides are analytic mindset together with dedication to work. I am inspired by great ideas and like people, thinking out of the box

Here is the summary of qualifications I have:

Model organisms and cell lines: Physcomitrium patens, Arabidopsis thaliana, E. coli, Ensifer meliloti, Medicago sativa, Trypanosoma brucei, Vibrio sp.; HeLa cells.
Generation of mutant and expression constructs in most of these organisms from the design of primers to verified lines. Maintenance, sterile work with plant, human cell culture, as well as bacteria culture; skills at BSL3-4 levels (certified in Russia, not verified in Europe).

Molecular biology:
DNA: all kinds of work with DNA, from extraction and genotyping to cloning and DNA methylation profiling (bisulfite sequencing), CRISPR-Cas9.
RNA: RNA-seq: from tissue RNA extraction to differential expressed genes, including low-input, picograms amounts of RNA protocols (SmartSeq2 conversion); RACE, and RT-PCR;
Protein: from the design of the construct to purification from E.coli by FPLC or gravity flow

Microscopy: BF, epifluorescence, confocal, airyscan; image analysis (counting, area of interest comparison by masking and other using FIJI; FIJI automatization)

In silico:
- bioinformatics: Multiple alignments (Clustal, Blast) and phylogenetic trees construction with JalView, MEGA, UGENE. Analysis of RNA-Seq data from raw reads to DGE values; analysis of bisulfite sequencing data from raw reads to DMRs. Genome browsers: SeqMonk, IGV, IGB
- genetic engineering: restriction/digestion molecular cloning, Gateway/AQUA cloning; workflow from competent cell preparation to the ready construct. CRISPR-Cas9 design and application or mutagenesis. Fusion (over)expression constructs; very good knowledge of SnapGene and Benching in-built molecular genetics tools.
- programming for biology: Unix independent user, bash/shell scripting. Conda environments and SnakeMake. Perl, and R (RStudio) basic programming. Confidence in Python and BioPython. Working with IDE (PyCharm), Jupiter notebooks. Active user of GitHub and Galaxy; NCBI, EBI, and Expasy tools; NCBI e-utilities, biomart
- statistics and graphics: RStudio (deseq2, edgeR, different plotting tools), Python (Pandas, Matplotlib, seaborn); image analysis with FIJI (ImageJ), automatization of processes

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis       Copy PMIDs To Clipboard

  1. Misra CS, Sousa AGG, Barros PM, Kermanov A, Becker JD. Cell-type-specific alternative splicing in the Arabidopsis germline. Plant Physiol. 2023 May 02; 192(1):85-101. PMID: 36515615; PMCID: PMC10152659; DOI: 10.1093/plphys/kiac574;
  2. Kuleshov KV, Vodop'ianov SO, Dedkov VG, Markelov ML, Kermanov AV, Kruglikov VD, Vodop'ianov AS, Pisanov RV, Chemisova OS, Mazrukho AB, Titova SV, Shipulin GA. Draft Genome Sequencing of Vibrio cholerae O1 El Tor Isolates Collected in the Russian Federation from Imported Cholera Cases. Genome Announc. 2014 Jul 17; 2(4). PMID: 25035324; PMCID: PMC4102861; DOI: 10.1128/genomeA.00624-14;
  3. Kuleshov KV, Vodop'ianov SO, Markelov ML, Dedkov VG, Kermanov AV, Kruglikov VD, Vodop'ianov AS, Pisanov RV, Mazrukho AB, Shipulin GA. Draft Genome Sequences of Vibrio cholerae O1 ElTor Strains 2011EL-301 and P-18785, Isolated in Russia. Genome Announc. 2013 Aug 22; 1(4). PMID: 23969060; PMCID: PMC3751615; DOI: 10.1128/genomeA.00659-13;
  4. Kuleshov KV, Markelov ML, Dedkov VG, Vodop'ianov AS, Kermanov AV, Pisanov RV, Kruglikov VD, Mazrukho AB, Maleev VV, Shipulin GA. [Phylogenetic analysis of genomes of Vibrio cholerae strains isolated on the territory of Rostov region]. Zh Mikrobiol Epidemiol Immunobiol. 2013; (6):13-20. PMID: 24605670
  5. Alekseeva LP, Telesmanich NR, Lomov IuM, Khramov MV, Kruglikov VD, Agafonova VV, Fateeva OF, Chebotarev DA, Kermanov AV. [Comparative assessment of dot-immunoassay and immunochromatography methods for detection of 01 serogroup of vibrio cholerae]. Zh Mikrobiol Epidemiol Immunobiol. 2010; (6):88-93. PMID: 21381382

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 5 publications over 4 distinct years, with a maximum of 2 publications in 2013


2017-2021 Portuguese Foundation for Science and Technology: individual PhD grant awarded
2014-2015 Finnish Center for International Mobility: Individual fellowship for the research stay in Finland in the lab of choice
2014-2015 Eranet Plus consortium: Individual grant for the research stay in the university of choice in EU
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