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Search Results to David Atkinson, PhD

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One or more keywords matched the following properties of Atkinson, David

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Research Expertise & Professional Interests The long standing objectives of my research are to provide the detailed structural and dynamic description of the plasma lipoproteins and apolipoproteins that is crucial to understanding the molecular mechanisms of such physiological processes as lipoprotein formation, receptor interactions, lipoprotein inter-conversions, and apoprotein exchange together with the changes in these characteristics that underlie the pathophysiology of atherosclerosis. Our research has been a component of a Program Project since its inception in 1980 and I have led the Program since 2001, taking over from Dr. Small. Building on initial training in diffraction methods and biophysics, I have maintained and expanded my expertise in state-of-the-art methods of molecular biophysics and structural biology including crystallography, electron microscopy/image processing, calorimetry and thermodynamics, circular dichroism, and molecular modeling/mechanics to probe the structure-function relationships of the lipoproteins and apolipoproteins. This includes a one year sabbatical at the MRC Laboratory of Molecular Biology, Cambridge, England developing electron microscopy. My long standing research program has involved many collaborations with current and past Program Project investigators, particularly Drs. Gursky, Small, and McKnight. Our previous work over more than three decades has focused on the structural and thermodynamic properties of specific lipoproteins (HDL and LDL), and apolipoproteins, particularly apoA-1, together with studies of the LDL receptor. We derived the first structural description of HDL, nascent HDL and LDL using x-ray methods. Our mutation studies of the conformation, stability, and lipid binding properties have contributed to providing a framework for understanding the molecular properties of apoA-1. Furthermore, our studies of peptides representing segments of apoA-1, together with “idealized” sequence models, have provided information on the role of specific residues and domains, and their interactions in the structure and stability of apoA-1. For LDL, we pioneered the use of cryo-electron microscopy to study LDL structure and used mAb labeling to investigate the topology of apoB. In collaborations with Dr. Graham Shipley, a long standing collaborator and colleague, our approach for the LDL receptor has focused on structural studies of the functional extracellular domain of the receptor reconstituted into lipid vesicles.
Self-Described Keywords Structural Biology
Self-Described Keywords structural electron microscopy

One or more keywords matched the following items that are connected to Atkinson, David

Item TypeName
Concept Microscopy, Electron
Concept Models, Structural
Academic Article Cryoelectron microscopy of low density lipoprotein in vitreous ice.
Academic Article Characterization of the lipoproteins of atherosclerotic swine.
Academic Article Structural organization of the lipoprotein HDLc from atherosclerotic swine. Structural features relating the particle surface and core.
Academic Article Structural studies of N- and C-terminally truncated human apolipoprotein A-I.
Academic Article Lipid-binding studies of human apolipoprotein A-I and its terminally truncated mutants.
Academic Article Biophysical properties of apolipoprotein E4 variants: implications in molecular mechanisms of correction of hypertriglyceridemia.
Academic Article Synthetic high density lipoprotein particles. Application to studies of the apoprotein specificity for selective uptake of cholesterol esters.
Academic Article The complete sequence and structural analysis of human apolipoprotein B-100: relationship between apoB-100 and apoB-48 forms.
Academic Article Structural basis for distinct functions of the naturally occurring Cys mutants of human apolipoprotein A-I.
Academic Article Apolipoprotein structure: crystals and models
Academic Article Structure and morphology of phosphatidylserine dispersions.
Academic Article The structure, physical and chemical properties of the soy bean protein glycinin.
Academic Article Structural studies of plasma lipoproteins.
Academic Article Reassembled plasma low density lipoproteins. Phospholipid-cholesterol ester-apoprotein B complexes.
Academic Article Thermal transitions and structural properties of synthetic cholesterol alkyl and alkenyl ethers: analogues of biological cholesterol esters.
Academic Article Solubilization of low-density lipoprotein with sodium deoxycholate and recombination of apoprotein B with dimyristoylphosphatidylcholine.
Academic Article Physical studies of d less than 1.006 g/ml lymph lipoproteins from rats fed palmitate-rich diets.

Highlights
Search Criteria
  • structural
  • electron microscopy