Search Results to David Atkinson, PhD

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Research Expertise & Professional Interests The long standing objectives of my research are to provide the detailed structural and dynamic description of the plasma lipoproteins and apolipoproteins that is crucial to understanding the molecular mechanisms of such physiological processes as lipoprotein formation, receptor interactions, lipoprotein inter-conversions, and apoprotein exchange together with the changes in these characteristics that underlie the pathophysiology of atherosclerosis. Our research has been a component of a Program Project since its inception in 1980 and I have led the Program since 2001, taking over from Dr. Small. Building on initial training in diffraction methods and biophysics, I have maintained and expanded my expertise in state-of-the-art methods of molecular biophysics and structural biology including crystallography, electron microscopy/image processing, calorimetry and thermodynamics, circular dichroism, and molecular modeling/mechanics to probe the structure-function relationships of the lipoproteins and apolipoproteins. This includes a one year sabbatical at the MRC Laboratory of Molecular Biology, Cambridge, England developing electron microscopy. My long standing research program has involved many collaborations with current and past Program Project investigators, particularly Drs. Gursky, Small, and McKnight. Our previous work over more than three decades has focused on the structural and thermodynamic properties of specific lipoproteins (HDL and LDL), and apolipoproteins, particularly apoA-1, together with studies of the LDL receptor. We derived the first structural description of HDL, nascent HDL and LDL using x-ray methods. Our mutation studies of the conformation, stability, and lipid binding properties have contributed to providing a framework for understanding the molecular properties of apoA-1. Furthermore, our studies of peptides representing segments of apoA-1, together with “idealized” sequence models, have provided information on the role of specific residues and domains, and their interactions in the structure and stability of apoA-1. For LDL, we pioneered the use of cryo-electron microscopy to study LDL structure and used mAb labeling to investigate the topology of apoB. In collaborations with Dr. Graham Shipley, a long standing collaborator and colleague, our approach for the LDL receptor has focused on structural studies of the functional extracellular domain of the receptor reconstituted into lipid vesicles.
Self-Described Keywords lipoproteins

One or more keywords matched the following items that are connected to Atkinson, David

Item TypeName
Concept Lipoproteins
Concept Lipoproteins, HDL
Concept Lipoproteins, LDL
Concept Lipoproteins, VLDL
Concept Receptors, Lipoprotein
Concept High-Density Lipoproteins, Pre-beta
Academic Article X-ray and neutron scattering studies of plasma lipoproteins.
Academic Article Organization of the core lipids of high density lipoproteins in the lactating bovine.
Academic Article Cryoelectron microscopy of low density lipoprotein in vitreous ice.
Academic Article Interaction of apoprotein from porcine high-density lipoprotein with dimyristoyl lecithin. 1. The structure of the complexes.
Academic Article Interaction of apoprotein from porcine high-density lipoprotein with dimyristoly lecithin. 2. Nature of lipid-protein interaction.
Academic Article Structure of human plasma low-density lipoproteins: molecular organization of the central core.
Academic Article Characterization of the lipoproteins of atherosclerotic swine.
Academic Article Structural organization of the lipoprotein HDLc from atherosclerotic swine. Structural features relating the particle surface and core.
Academic Article Studies on the structure of low density lipoproteins isolated from Macaca fascicularis fed an atherogenic diet.
Academic Article The interfacial properties of ApoA-I and an amphipathic alpha-helix consensus peptide of exchangeable apolipoproteins at the triolein/water interface.
Academic Article Structure and stability of apolipoprotein a-I in solution and in discoidal high-density lipoprotein probed by double charge ablation and deletion mutation.
Academic Article Biophysical properties of apolipoprotein E4 variants: implications in molecular mechanisms of correction of hypertriglyceridemia.
Academic Article Phospholipid/cholesteryl ester microemulsions containing unesterified cholesterol: model systems for low density lipoproteins.
Academic Article Reconstituting initial events during the assembly of apolipoprotein B-containing lipoproteins in a cell-free system.
Academic Article Human LDL core cholesterol ester packing: three-dimensional image reconstruction and SAXS simulation studies.
Academic Article Enhancing the contrast of ApoB to locate the surface components in the 3D density map of human LDL.
Academic Article Crystal structure of C-terminal truncated apolipoprotein A-I reveals the assembly of high density lipoprotein (HDL) by dimerization.
Academic Article Synthetic high density lipoprotein particles. Application to studies of the apoprotein specificity for selective uptake of cholesterol esters.
Academic Article Immuno-electron cryo-microscopy imaging reveals a looped topology of apoB at the surface of human LDL.
Academic Article Application of Circular Dichroism to Lipoproteins: Structure, Stability and Remodeling of Good and Bad Cholesterol
Academic Article Lipid organization in human plasma low density lipoproteins
Academic Article Recombinant lipoproteins: implications for structure and assembly of native lipoproteins.
Academic Article Reassembly of low-density lipoproteins.
Academic Article The structure of a high density lipoprotein (HDL3) from porcine plasma.
Academic Article Small-angle x-ray scattering of human serum high-density lipoproteins.
Academic Article Influence of different molecular species of phosphatidylcholine on cholesterol transport from lipoprotein recombinants in the rat.
Academic Article Physical properties of apoprotein B in mixed micelles with sodium deoxycholate and in a vesicle with dimyristoyl phosphatidylcholine.
Academic Article Structural studies of plasma lipoproteins.
Academic Article Reassembled plasma low density lipoproteins. Phospholipid-cholesterol ester-apoprotein B complexes.
Academic Article Solubilization of low-density lipoprotein with sodium deoxycholate and recombination of apoprotein B with dimyristoylphosphatidylcholine.
Academic Article Physical studies of d less than 1.006 g/ml lymph lipoproteins from rats fed palmitate-rich diets.
Academic Article Microemulsions of phospholipids and cholesterol esters. Protein-free models of low density lipoprotein.
Academic Article Physical characterization of lymph chylomicrons and very low density lipoproteins from nonhuman primates fed saturated dietary fat.
Academic Article Surface behavior of apolipoprotein A-I and its deletion mutants at model lipoprotein interfaces.
Academic Article Lipid-free Apolipoprotein A-I Structure: Insights into HDL Formation and Atherosclerosis Development.
Academic Article Probing the C-terminal domain of lipid-free apoA-I demonstrates the vital role of the H10B sequence repeat in HDL formation.
Academic Article Increased Binding of Apolipoproteins A-I and E4 to Triglyceride-Rich Lipoproteins is linked to Induction of Hypertriglyceridemia.
Academic Article A consensus model of human apolipoprotein A-I in its monomeric and lipid-free state.
Academic Article N-terminal mutation of apoA-I and interaction with ABCA1 reveal mechanisms of nascent HDL biogenesis.

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