Search Results to Bjorn Reinhard, Dr. rer. nat.

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Research Expertise & Professional Interests Research in the Reinhard Lab focuses on new optical materials and their application to interrogate fundamental life processes. We are exploring the interface between nanotechnology and biological systems. For an overview of current research projects, please visit our group’s website. Recents techniques/materials developed in the Nano-Bio Interface Lab include: Plasmon ruler RNase A cleavage assay: (A) The RNA plasmon rulers are bound to the surface of a glass flow chamber using a BSA (bovin serum albumin)-Biotin-NeutrAvidin surface chemistry. Upon addition of RNase A, the RNA tether is cleaved, and the dimer converted into a monomer. (B) Single RNA plasmon ruler cleavage trajectory (recorded at 96 Hz). (I) The plasmon ruler is first incubated in buffer containing spermidine at defined concentrations (0 -5 mM), (II) the buffer is exchanged with a 1 nM RNase A solution, causing (III) a strong drop in intensity upon RNA cleavage. Inset: Number of cleavage events for flushing with/without enzyme. ?tcl is defined as the time between enzyme addition and cleavage. For more information, refer to: L.R. Skewis & B.M. Reinhard, Nano Lett., 8, 214 (2008). Plasmon Coupling Microscopy: Gold nanoparticle labeled surface receptors (left) and spectral signature (right) as function of interparticle distance. (a) For interparticle separations ? larger than the particle diameter D, the near-field interactions between the particles is small and the resonance wavelength ?res is that of an individual particle. (b) For interparticle separations ? < D the plasmons in the individual particles couple and the resonance wavelength ?res red-shifts with decreasing separation. This spectral shift is observable as an increase in the intensity ratio R = I580nm/I530nm. Multiscale Nanoparticle Cluster Arrays (NCAs): SEM images from extracts of nanoparticle cluster arrays with varying diameters of e-beam defined binding size D = 50 nm (a), 80 nm (b), 100 nm (c), 130 nm (d), 200 nm (e). The SEM images confirm that through control of the diameter of the e-beam fabricated binding site the cluster size can be continuously varied. The enlargement of an individual cluster in (f) shows junctions and crevices between nearly touching particles constituting a high degree of roughness on the nanoscale. The Reinhard group utilizes a variety of techniques including: -Spectroscopy: Raman, SERS, Single Molecule Fluorescence, Plasmon -Live Cell Imaging -Nanofabrication -Transmission Electron Microscopy -Scanning Electron Microscopy -Cell Culture Facility -Fluorescence Plate Readers -Nanoparticle Synthesis/Functionalization/Integration -The Photonics Center -Center of Nanoscience and Nanobiotechnology
Self-Described Keywords Cell Membrane Organization and Signaling

One or more keywords matched the following items that are connected to Reinhard, Bjorn

Item TypeName
Concept Cell Differentiation
Concept Cell Line
Concept Cell Membrane
Concept Cell Survival
Concept Cells, Cultured
Concept Dendritic Cells
Concept Diagnostic Imaging
Concept HeLa Cells
Concept Neoplastic Stem Cells
Concept Cell Adhesion Molecules
Concept Caco-2 Cells
Concept Cell Line, Tumor
Concept HEK293 Cells
Concept Primary Cell Culture
Concept MCF-7 Cells
Academic Article HIV-1 incorporation of host-cell-derived glycosphingolipid GM3 allows for capture by mature dendritic cells.
Academic Article Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169.
Academic Article Nanoparticle-induced apoptosis propagates through hydrogen-peroxide-mediated bystander killing: insights from a human intestinal epithelium in vitro model.
Academic Article Control of colloid surface chemistry through matrix confinement: facile preparation of stable antibody functionalized silver nanoparticles.
Academic Article Resolving sub-diffraction limit encounters in nanoparticle tracking using live cell plasmon coupling microscopy.
Academic Article Insights from a nanoparticle minuet: two-dimensional membrane profiling through silver plasmon ruler tracking.
Academic Article Monitoring enzymatic degradation of pericellular matrices through SERS stamping.
Academic Article Quantification of differential ErbB1 and ErbB2 cell surface expression and spatial nanoclustering through plasmon coupling.
Academic Article Monitoring the size and lateral dynamics of ErbB1 enriched membrane domains through live cell plasmon coupling microscopy.
Academic Article Scavenger receptor mediated endocytosis of silver nanoparticles into J774A.1 macrophages is heterogeneous.
Academic Article Illuminating epidermal growth factor receptor densities on filopodia through plasmon coupling.
Academic Article Illuminating the lateral organization of cell-surface CD24 and CD44 through plasmon coupling between Au nanoparticle immunolabels.
Academic Article Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells.
Academic Article Dressing up Nanoparticles: A Membrane Wrap to Induce Formation of the Virological Synapse.
Academic Article Probing subdiffraction limit separations with plasmon coupling microscopy: concepts and applications.
Academic Article Nanoconjugation prolongs endosomal signaling of the epidermal growth factor receptor and enhances apoptosis.
Academic Article Tumor Cell-Derived Periostin Regulates Cytokines That Maintain Breast Cancer Stem Cells.
Academic Article Access of HIV-2 to CD169-dependent dendritic cell-mediated trans infection pathway is attenuated.
Academic Article Lipid-Mediated Targeting with Membrane-Wrapped Nanoparticles in the Presence of Corona Formation.
Academic Article Plasmonic Enhancement of Selective Photonic Virus Inactivation.
Academic Article Nanoparticle-cell interactions induced apoptosis: a case study with nanoconjugated epidermal growth factor.
Grant CAS-MNP: Elucidating Nanoplastics - Cell Interactions that Enhance Polycyclic Aromatic Hydrocarbon Uptake in an Intestinal Membrane Model

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