William Lehman | Profiles RNS

William J. Lehman, PhD

Professor

Boston University Chobanian & Avedisian School of Medicine

Pharmacology, Physiology & Biophysics

PhD, Princeton University
BS, Stony Brook University

Websites

	Whitaker Cardiovascular Institute's Training Program
	Google Scholar
	ResearchGate
	Academia

ORCID : 0000-0002-9430-9521

We are involved in structural studies on the assembly and function of actin-containing thin filaments in muscle and non-muscle cells. Our principal goal is to analyze and elucidate the mechanisms of thin filament-linked regulation of muscle contraction and cytoskeletal remodeling. To accomplish this goal, we use a combination of molecular biology, electron microscopy, electron tomography, image reconstruction and computational tools such as molecular dynamics protocols to better understand the interactions and dynamics of protein components of isolated and reconstituted thin filaments. Studies on mutants are carried out to elucidate abnormal filament function in disease processes. We have an excellent track record in successfully educating graduate and post-doctoral students in the application of the state-of-the-art techniques that we use. In particular, we have trained students with backgrounds in biological and biochemical sciences to be fearless about the challenge of performing sophisticated biophysical approaches, and, conversely, teaching students with background in physical and computational sciences to understand the biomedical underpinnings of our work. This dual process of training students with these diverse backgrounds in one laboratory setting is synergistic. As a sign of our success: of the 16 papers that have been published by us between 2018 and now (2021), 14 were co-authored by 8 different current or former post-doctoral fellows and graduate students from my laboratory.

Our laboratory was the first to directly visualize the steric-blocking mechanism of muscle regulation by identifying the positions assumed by tropomyosin on actin in the presence and the absence of Ca2+ using cryo-electron microscopy and negative staining. We also have demonstrated that during muscle activation tropomyosin moves away from myosin cross-bridge binding sites on actin in two highly cooperative steps, one induced by Ca2+ binding to troponin and a second induced by the binding of myosin to actin. Our laboratory is continuing the above-mentioned studies to obtain even greater resolution of the processes involved. At the same time, we are investigating the structural organization of troponin on thin filaments and the changes it undergoes on binding of Ca2+. We have also been engaged in studies on the structural interactions of other actin binding proteins including a-actinin, myosin binding protein-C, caldesmon, calponin, cortactin, filamin and native and mutant dystrophin, namely proteins that play important roles in the organization of the cytoskeleton in striated and smooth muscles as well as in non-muscle cells. (03/17/2021)

Mentor for Graduate Medical Students
Boston Medical Center
Medical Sciences

BU Research
NIH RePORTER Research

Thin Filaments and Muscle Regulation
02/15/2022 - 01/31/2026 (PI)
NIH/National Heart, Lung, and Blood Institute
5R01HL036153-33

Pathogenesis and in vivo suppression of thin filament based cardiomyopathies
01/01/2022 - 12/31/2023 (Subcontract PI)
Johns Hopkins University NIH NHLBI
2R01HL124091-05A1

Revealing Pathomechanisms of Mutant TPM1 Through a Hybrid Computational-Experimental Approach
07/10/2017 - 06/30/2022 (Subcontract PI)
Yale University NIH NHLBI
5R01HL136590-05

Thin Filaments and Muscle Regulation
01/01/2018 - 12/31/2021 (PI)
NIH/National Heart, Lung, and Blood Institute
5R01HL036153-31

Cooperativity in Cardiac Myofilament Interactome in Health and Disease
08/11/2016 - 05/31/2020 (Subcontract PI)
University of Massachusetts, Lowell NIH NHLBI
5R01HL123774-02

X-Ray Crystallography of TPM3.1 N- and C-terminal peptides
12/01/2018 - 09/30/2019 (PI)
TroBio Therapeutics Pty Ltd

Targeting Tropomyosin as a Novel Anti-Cancer Therapy
12/01/2017 - 11/30/2018 (Subcontract PI)
Kazia Therapeutics Ltd Australian Cmlth Gov

Thin Filaments and Muscle Regulation
12/01/2015 - 12/31/2017 (PI)
NIH/National Heart, Lung, and Blood Institute
5R37HL036153-27

Structural Analysis of Anticancer Compound Targets on Tpm3.1 Tropomyosin
04/01/2016 - 06/30/2017 (PI)
Novogen

Thin Filaments and Muscle Regulation
12/07/2012 - 11/30/2016 (PI)
NIH/National Heart, Lung, and Blood Institute
5R37HL036153-25

Showing 10 of 14 results. Show All Results

Title

Yr	Title	Project-Sub Proj	Pubs
2024	Thin Filaments and Muscle Regulation	5R01HL036153-34
2023	Computational Pipeline for Identification of Disease-Causing Variants in Genes of the Cardiac Sarcomere	2R01HL136590-06
2023	Thin Filaments and Muscle Regulation	5R01HL036153-33
2022	Thin Filaments and Muscle Regulation	2R01HL036153-32
2021	Thin Filaments and Muscle Regulation	5R01HL036153-31	61
2020	Thin Filaments and Muscle Regulation	5R01HL036153-30	61
2019	Thin Filaments and Muscle Regulation	5R01HL036153-29	61
2018	Thin Filaments and Muscle Regulation	2R01HL036153-28	61
2017	Thin Filaments and Muscle Regulation	5R37HL036153-27	73
2016	Thin Filaments and Muscle Regulation	4R37HL036153-26	73

Showing 10 of 41 results. Show All Results

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis Copy PMIDs To Clipboard

Rynkiewicz MJ, Pavadai E, Lehman W. Modeling Human Cardiac Thin Filament Structures. Front Physiol. 2022; 13:932333.View Related Profiles.

Rynkiewicz, Michael
Lehman, William

PMID: 35812320; PMCID: PMC9257132; DOI: 10.3389/fphys.2022.932333;

Pavadai E, Rynkiewicz MJ, Yang Z, Gould IR, Marston SB, Lehman W. Modulation of cardiac thin filament structure by phosphorylated troponin-I analyzed by protein-protein docking and molecular dynamics simulation. Arch Biochem Biophys. 2022 Aug 15; 725:109282.View Related Profiles.

Rynkiewicz, Michael
Lehman, William

PMID: 35577070; PMCID: PMC10680062; DOI: 10.1016/j.abb.2022.109282;

Suphamungmee W, Lehman W, Morgan KG. Functional Remodeling of the Contractile Smooth Muscle Cell Cortex, a Provocative Concept, Supported by Direct Visualization of Cortical Remodeling. Biology (Basel). 2022 Apr 26; 11(5).View Related Profiles.

Morgan, Kathleen
Lehman, William
Suphamungmee, Worawit

PMID: 35625390; PMCID: PMC9138025; DOI: 10.3390/biology11050662;

Teekakirikul P, Zhu W, Xu X, Young CB, Tan T, Smith AM, Wang C, Peterson KA, Gabriel GC, Ho S, Sheng Y, Moreau de Bellaing A, Sonnenberg DA, Lin JH, Fotiou E, Tenin G, Wang MX, Wu YL, Feinstein T, Devine W, Gou H, Bais AS, Glennon BJ, Zahid M, Wong TC, Ahmad F, Rynkiewicz MJ, Lehman WJ, Keavney B, Alastalo TP, Freckmann ML, Orwig K, Murray S, Ware SM, Zhao H, Feingold B, Lo CW. Genetic resiliency associated with dominant lethal TPM1 mutation causing atrial septal defect with high heritability. Cell Rep Med. 2022 02 15; 3(2):100501.View Related Profiles.

Rynkiewicz, Michael
Lehman, William

PMID: 35243414; PMCID: PMC8861813; DOI: 10.1016/j.xcrm.2021.100501;

Doran MH, Lehman W. The Central Role of the F-Actin Surface in Myosin Force Generation. Biology (Basel). 2021 Nov 23; 10(12). PMID: 34943138; PMCID: PMC8698748; DOI: 10.3390/biology10121221;

Lehman W, Pavadai E, Rynkiewicz MJ. C-terminal troponin-I residues trap tropomyosin in the muscle thin filament blocked-state. Biochem Biophys Res Commun. 2021 04 30; 551:27-32.View Related Profiles.

Rynkiewicz, Michael
Lehman, William

PMID: 33714756; PMCID: PMC8026701; DOI: 10.1016/j.bbrc.2021.03.010;

Doran MH, Pavadai E, Rynkiewicz MJ, Walklate J, Bullitt E, Moore JR, Regnier M, Geeves MA, Lehman W. Cryo-EM and Molecular Docking Shows Myosin Loop 4 Contacts Actin and Tropomyosin on Thin Filaments. Biophys J. 2020 08 18; 119(4):821-830.View Related Profiles.

Bullitt, Esther
Moore, Jeffrey
Rynkiewicz, Michael
Lehman, William

PMID: 32730789; PMCID: PMC7451941; DOI: 10.1016/j.bpj.2020.07.006;

Pavadai E, Lehman W, Rynkiewicz MJ. Protein-Protein Docking Reveals Dynamic Interactions of Tropomyosin on Actin Filaments. Biophys J. 2020 07 07; 119(1):75-86.View Related Profiles.

Rynkiewicz, Michael
Lehman, William

PMID: 32521240; PMCID: PMC7335911; DOI: 10.1016/j.bpj.2020.05.017;

Lehman W, Maéda Y. Introducing a special issue of the Journal of Muscle Research and Cell Motility on actin and actin-binding proteins. J Muscle Res Cell Motil. 2020 03; 41(1):1-2. PMID: 31865487

Pavadai E, Rynkiewicz MJ, Ghosh A, Lehman W. Docking Troponin T onto the Tropomyosin Overlapping Domain of Thin Filaments. Biophys J. 2020 01 21; 118(2):325-336.View Related Profiles.

Ghosh, Anita
Rynkiewicz, Michael
Lehman, William

PMID: 31864661; PMCID: PMC6976810; DOI: 10.1016/j.bpj.2019.11.3393;

Showing 10 of 182 results. Show More

Egge N, Arneaud SLB, Wales P, Mihelakis M, McClendon J, Fonseca RS, Savelle C, Gonzalez I, Ghorashi A, Yadavalli S, Lehman WJ, Mirzaei H, Douglas PM. Age-Onset Phosphorylation of a Minor Actin Variant Promotes Intestinal Barrier Dysfunction. Dev Cell. 2019 12 02; 51(5):587-601.e7. PMID: 31794717; PMCID: PMC6897307; DOI: 10.1016/j.devcel.2019.11.001;

Janco M, Rynkiewicz MJ, Li L, Hook J, Eiffe E, Ghosh A, Böcking T, Lehman WJ, Hardeman EC, Gunning PW. Molecular integration of the anti-tropomyosin compound ATM-3507 into the coiled coil overlap region of the cancer-associated Tpm3.1. Sci Rep. 2019 08 02; 9(1):11262.View Related Profiles.

Ghosh, Anita
Rynkiewicz, Michael
Lehman, William

PMID: 31375704; PMCID: PMC6677793; DOI: 10.1038/s41598-019-47592-9;

Lehman W, Moore JR, Campbell SG, Rynkiewicz MJ. The Effect of Tropomyosin Mutations on Actin-Tropomyosin Binding: In Search of Lost Time. Biophys J. 2019 06 18; 116(12):2275-2284.View Related Profiles.

Moore, Jeffrey
Rynkiewicz, Michael
Lehman, William

PMID: 31130236; PMCID: PMC6588729; DOI: 10.1016/j.bpj.2019.05.009;

Lehman W, Rynkiewicz MJ, Moore JR. A new twist on tropomyosin binding to actin filaments: perspectives on thin filament function, assembly and biomechanics. J Muscle Res Cell Motil. 2020 03; 41(1):23-38.View Related Profiles.

Moore, Jeffrey
Rynkiewicz, Michael
Lehman, William

PMID: 30771202; PMCID: PMC6697252; DOI: 10.1007/s10974-019-09501-5;

Kiani FA, Lehman W, Fischer S, Rynkiewicz MJ. Spontaneous transitions of actin-bound tropomyosin toward blocked and closed states. J Gen Physiol. 2019 01 07; 151(1):4-8.View Related Profiles.

Rynkiewicz, Michael
Lehman, William

PMID: 30442774; PMCID: PMC6314389; DOI: 10.1085/jgp.201812188;

Lehman W, Li X, Kiani FA, Moore JR, Campbell SG, Fischer S, Rynkiewicz MJ. Precise Binding of Tropomyosin on Actin Involves Sequence-Dependent Variance in Coiled-Coil Twisting. Biophys J. 2018 09 18; 115(6):1082-1092.View Related Profiles.

Moore, Jeffrey
Rynkiewicz, Michael
Lehman, William

PMID: 30195938; PMCID: PMC6139885; DOI: 10.1016/j.bpj.2018.08.017;

Farman GP, Rynkiewicz MJ, Orzechowski M, Lehman W, Moore JR. HCM and DCM cardiomyopathy-linked a-tropomyosin mutations influence off-state stability and crossbridge interaction on thin filaments. Arch Biochem Biophys. 2018 06 01; 647:84-92.View Related Profiles.

Moore, Jeffrey
Orzechowski, Marek
Rynkiewicz, Michael
Lehman, William

PMID: 29626422; PMCID: PMC5958618; DOI: 10.1016/j.abb.2018.04.002;

Sewanan L, Park J, Rynkiewicz MJ, Hollenberg SM, Papoutsidakis N, Jacoby D, Moore JR, Lehman W, Qyang Y, Campbell SG. Pathogenic Mechanisms of the Cardiomyopathy-Associated Alpha-Tropomyosin Variant E192K as Revealed by Multiscale Modeling and Experiments. Biophysical Journal. 2018; 114(3):495a. View Publication
Viswanathan MC, Schmidt W, Madan A, Sullivan LC, Newhard CS, Rynkiewicz MJ, Lehman W, Swank DM, Cammarato A. The ACTC M305L Hypertrophic Cardiomyopathy Mutation Results in Hypercontractility and Impaired Relaxation of Drosophila Muscles. Biophysical Journal. 2018; 114(3):315a. View Publication
Kiani FA, Rynkiewicz MJ, Fischer S, Lehman W. Tropomyosin Translocation on F-Actin Revealed by Molecular Dynamics Simulations. Biophysical Journal. 2018; 114(3):136a. View Publication

Rynkiewicz MJ, Prum T, Hollenberg S, Kiani FA, Fagnant PM, Marston SB, Trybus KM, Fischer S, Moore JR, Lehman W. Tropomyosin Must Interact Weakly with Actin to Effectively Regulate Thin Filament Function. Biophys J. 2017 Dec 05; 113(11):2444-2451.View Related Profiles.

Moore, Jeffrey
Rynkiewicz, Michael
Lehman, William

PMID: 29211998; PMCID: PMC5768522; DOI: 10.1016/j.bpj.2017.10.004;

Viswanathan MC, Schmidt W, Rynkiewicz MJ, Agarwal K, Gao J, Katz J, Lehman W, Cammarato A. Distortion of the Actin A-Triad Results in Contractile Disinhibition and Cardiomyopathy. Cell Rep. 2017 Sep 12; 20(11):2612-2625.View Related Profiles.

Rynkiewicz, Michael
Lehman, William

PMID: 28903042; PMCID: PMC5902318; DOI: 10.1016/j.celrep.2017.08.070;

Lehman W. Switching Muscles On and Off in Steps: The McKillop-Geeves Three-State Model of Muscle Regulation. Biophys J. 2017 06 20; 112(12):2459-2466. PMID: 28552313; PMCID: PMC5479050; DOI: 10.1016/j.bpj.2017.04.053;

Sewanan L, Park J, Ren Y, Urdaneta A, Rynkiewicz MJ, Moore JR, Lehman W, Jacoby D, Qyang Y, Campbell S. Investigating the Phenotype of Cardiomyopathy-Associated Alpha-Tropomyosin E192K Mutation in Patient-Derived Engineered Heart Tissue. Biophysical Journal. 2017; 112(3):260a. View Publication
Rynkiewicz MJ, Stehn J, Gunning PW, Lehman W. Structure of the Anti-Cancer Compound TR100 Target-Sites on Tpm3.1 Tropomyosin. Biophysical Journal. 2017; 112(3):560a. View Publication
Viswanathan MC, Schmidt W, Orzechowski M, Lehman W, Cammarato A. The Actc A295S Hypertrophic Cardiomyopathy Mutation Promotes Thin Filament Disinhibition and Enhances Contractile Activity. Biophysical Journal. 2017; 112(3):559a. View Publication
Moore JR, Prum T, Fagnant PM, Trybus KM, Lehman W. D292V Actin Mutation Stabilizes Tropomyosin in the Off-State of the Thin Filament. Biophysical Journal. 2017; 112(3):259a. View Publication

Sewanan LR, Moore JR, Lehman W, Campbell SG. Predicting Effects of Tropomyosin Mutations on Cardiac Muscle Contraction through Myofilament Modeling. Front Physiol. 2016; 7:473.View Related Profiles.

Moore, Jeffrey
Lehman, William

PMID: 27833562; PMCID: PMC5081029; DOI: 10.3389/fphys.2016.00473;

Rynkiewicz MJ, Fischer S, Lehman W. The propensity for tropomyosin twisting in the presence and absence of F-actin. Arch Biochem Biophys. 2016 Nov 01; 609:51-58.View Related Profiles.

Rynkiewicz, Michael
Lehman, William

PMID: 27663225; PMCID: PMC5064861; DOI: 10.1016/j.abb.2016.09.008;

Lehman W. Thin Filament Structure and the Steric Blocking Model. Compr Physiol. 2016 Mar 15; 6(2):1043-69. PMID: 27065174; DOI: 10.1002/cphy.c150030;

Moore JR, Campbell SG, Lehman W. Structural determinants of muscle thin filament cooperativity. Arch Biochem Biophys. 2016 Mar 15; 594:8-17.View Related Profiles.

Moore, Jeffrey
Lehman, William

PMID: 26891592; PMCID: PMC4792785; DOI: 10.1016/j.abb.2016.02.016;

Fischer S, Rynkiewicz MJ, Moore JR, Lehman W. Tropomyosin diffusion over actin subunits facilitates thin filament assembly. Struct Dyn. 2016 Jan; 3(1):012002.View Related Profiles.

Moore, Jeffrey
Rynkiewicz, Michael
Lehman, William

PMID: 26798831; PMCID: PMC4714992; DOI: 10.1063/1.4940223;

von der Ecken J, Muller M, Lehman W, Manstein DJ, Penczek P, Raunser S. European Microscopy Congress. Our muscle at near-atomic resolution - Cryo-EM structure of the F-actin-tropomyosin complex. 2016; 7-8. View Publication
von der Ecken J, Muller M, Lehman W, Manstein DJ, Penczek P, Raunser. Structure of the F-Actin-Tropomyosin Complex Revealed by Electron Cryomicroscopy. Biophysical Journal. 2016; 110(3):156a. View Publication
Rynkiewicz MJ, Schott V, Orzechowski M, Fischer S, Lehman W. Does Tropomyosin Slide or Roll over F-Actin during Regulatory Transitions?. Biophysical Journal. 2016; 110(3):524a. View Publication
Farman GP, Rynkiewicz MJ, Orzechowski M, Lehman W, Moore JR. Cardiomyopathy Linked Mutations in Alpha Tropomyosin Influence Blocked State Stability but not Myosin Strong Binding. Biophysical Journal. 2016; 110(3):124-125. View Publication

Rynkiewicz MJ, Schott V, Orzechowski M, Lehman W, Fischer S. Electrostatic interaction map reveals a new binding position for tropomyosin on F-actin. J Muscle Res Cell Motil. 2015 Dec; 36(6):525-33.View Related Profiles.

Orzechowski, Marek
Rynkiewicz, Michael
Lehman, William

PMID: 26286845; PMCID: PMC4760918; DOI: 10.1007/s10974-015-9419-z;

Alamo L, Li XE, Espinoza-Fonseca LM, Pinto A, Thomas DD, Lehman W, Padrón R. Tarantula myosin free head regulatory light chain phosphorylation stiffens N-terminal extension, releasing it and blocking its docking back. Mol Biosyst. 2015 Aug; 11(8):2180-9.View Related Profiles.

Lehman, William
Li, Xiaochuan Li

PMID: 26038302; PMCID: PMC4503497; DOI: 10.1039/c5mb00163c;

Schmidt WM, Lehman W, Moore JR. Direct observation of tropomyosin binding to actin filaments. Cytoskeleton (Hoboken). 2015 Jun; 72(6):292-303.View Related Profiles.

Moore, Jeffrey
Lehman, William
Schmidt, William

PMID: 26033920; PMCID: PMC4529784; DOI: 10.1002/cm.21225;

Begonja AJ, Pluthero FG, Suphamungmee W, Giannini S, Christensen H, Leung R, Lo RW, Nakamura F, Lehman W, Plomann M, Hoffmeister KM, Kahr WH, Hartwig JH, Falet H. FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets. Blood. 2015 Jul 2; 126(1):80-8.View Related Profiles.

Lehman, William
Suphamungmee, Worawit

PMID: 25838348; PMCID: PMC4492198; DOI: 10.1182/blood-2014-07-587600;

Lehman W, Medlock G, Li XE, Suphamungmee W, Tu AY, Schmidtmann A, Ujfalusi Z, Fischer S, Moore JR, Geeves MA, Regnier M. Phosphorylation of Ser283 enhances the stiffness of the tropomyosin head-to-tail overlap domain. Arch Biochem Biophys. 2015 Apr 1; 571:10-5.View Related Profiles.

Moore, Jeffrey
Lehman, William
Suphamungmee, Worawit
Li, Xiaochuan Li

PMID: 25726728; PMCID: PMC4385494; DOI: 10.1016/j.abb.2015.02.026;

Farman GP, Orzechowski M, Fischer S, Lehman W, Moore JR. Serine 61 Phosphorylation Rescues Cardiomyopathic Effects of Tropomyosin Mutation. Biophysical Journal. 2015; 108(2):446-447. View Publication

von der Ecken J, Müller M, Lehman W, Manstein DJ, Penczek PA, Raunser S. Structure of the F-actin-tropomyosin complex. Nature. 2015 Mar 5; 519(7541):114-7. PMID: 25470062; PMCID: PMC4477711; DOI: 10.1038/nature14033;

Orzechowski M, Fischer S, Moore JR, Lehman W, Farman GP. Energy landscapes reveal the myopathic effects of tropomyosin mutations. Arch Biochem Biophys. 2014 Dec 15; 564:89-99.View Related Profiles.

Moore, Jeffrey
Orzechowski, Marek
Lehman, William

PMID: 25241052; PMCID: PMC4258436; DOI: 10.1016/j.abb.2014.09.007;

Orzechowski M, Li XE, Fischer S, Lehman W. An atomic model of the tropomyosin cable on F-actin. Biophys J. 2014 Aug 5; 107(3):694-9.View Related Profiles.

Orzechowski, Marek
Lehman, William
Li, Xiaochuan Li

PMID: 25099808; PMCID: PMC4129500; DOI: 10.1016/j.bpj.2014.06.034;

Gu C, Chang J, Shchedrina VA, Pham VA, Hartwig JH, Suphamungmee W, Lehman W, Hyman BT, Bacskai BJ, Sever S. Regulation of dynamin oligomerization in cells: the role of dynamin-actin interactions and its GTPase activity. Traffic. 2014 Aug; 15(8):819-38.View Related Profiles.

Lehman, William
Suphamungmee, Worawit

PMID: 24891099; PMCID: PMC4107071; DOI: 10.1111/tra.12178;

Li XE, Orzechowski M, Lehman W, Fischer S. Structure and flexibility of the tropomyosin overlap junction. Biochem Biophys Res Commun. 2014 Mar 28; 446(1):304-8.View Related Profiles.

Orzechowski, Marek
Lehman, William
Li, Xiaochuan Li

PMID: 24607906; PMCID: PMC4003899; DOI: 10.1016/j.bbrc.2014.02.097;

Yang S, Barbu-Tudoran L, Orzechowski M, Craig R, Trinick J, White H, Lehman W. Three-dimensional organization of troponin on cardiac muscle thin filaments in the relaxed state. Biophys J. 2014 Feb 18; 106(4):855-64.View Related Profiles.

Orzechowski, Marek
Lehman, William

PMID: 24559988; PMCID: PMC3944806; DOI: 10.1016/j.bpj.2014.01.007;

Orzechowski M, Moore JR, Fischer S, Lehman W. Tropomyosin movement on F-actin during muscle activation explained by energy landscapes. Arch Biochem Biophys. 2014 Mar 1; 545:63-8.View Related Profiles.

Moore, Jeffrey
Orzechowski, Marek
Lehman, William

PMID: 24412204; PMCID: PMC3943668; DOI: 10.1016/j.abb.2014.01.001;

Schmidt WM, Wang CLA, Leavis PC, Lehman W, Moore JR. Direct Visualization of Tropomyosin Isoform Binding to F-Actin. Biophysical Journal. 2014; 106(2):165a. View Publication
Medlock G, Suphamungmee W, Tu AY, Li X, Regnier M, Geeves MA, Lehman W. Effects of Phosphomimetic Mutations on the Persistence Length and Thin Filament Binding Properties of a and ß-Tropomyosin. 58th Annual Meeting of the Biophysical Society. 2014. View Publication

Viswanathan MC, Kaushik G, Engler AJ, Lehman W, Cammarato A. A Drosophila melanogaster model of diastolic dysfunction and cardiomyopathy based on impaired troponin-T function. Circ Res. 2014 Jan 17; 114(2):e6-17. PMID: 24221941; PMCID: PMC4526186; DOI: 10.1161/CIRCRESAHA.114.302028;

Lehman W, Li XE, Orzechowski M, Fischer S. The structural dynamics of a-tropomyosin on F-actin shape the overlap complex between adjacent tropomyosin molecules. Arch Biochem Biophys. 2014 Jun 15; 552-553:68-73.View Related Profiles.

Orzechowski, Marek
Lehman, William
Li, Xiaochuan Li

PMID: 24071513; PMCID: PMC3962804; DOI: 10.1016/j.abb.2013.09.011;

Marston S, Memo M, Messer A, Papadaki M, Nowak K, McNamara E, Ong R, El-Mezgueldi M, Li X, Lehman W. Mutations in repeating structural motifs of tropomyosin cause gain of function in skeletal muscle myopathy patients. Hum Mol Genet. 2013 Dec 15; 22(24):4978-87. PMID: 23886664; PMCID: PMC3836477; DOI: 10.1093/hmg/ddt345;

Janco M, Suphamungmee W, Li X, Lehman W, Lehrer SS, Geeves MA. Polymorphism in tropomyosin structure and function. J Muscle Res Cell Motil. 2013 Aug; 34(3-4):177-87.View Related Profiles.

Lehman, William
Suphamungmee, Worawit

PMID: 23832280; PMCID: PMC4509547; DOI: 10.1007/s10974-013-9353-x;

Lehman W, Orzechowski M, Li XE, Fischer S, Raunser S. Gestalt-binding of tropomyosin on actin during thin filament activation. J Muscle Res Cell Motil. 2013 Aug; 34(3-4):155-63.View Related Profiles.

Orzechowski, Marek
Lehman, William
Li, Xiaochuan Li

PMID: 23666668; PMCID: PMC3773262; DOI: 10.1007/s10974-013-9342-0;

Suphamungmee W, Li X, Janco M, Geeves MA, Lehman W. Persistence Length of Tropomyosin Heterodimers Formed from Wild-Type and Mutant Chains. Biophysical Journal. 2013; 104(2):481a. View Publication
Li X, Orzechowski M, Fischer S, Lehman W. Tropomyosin Flexibility and Transitions between Thin Filament On- and Off-States. Biophysical Journal. 2013; 104(2):481a. View Publication
Marston S, Lehman W, Li X, Memo M. A Repeating Structural Motif in Tropomyosin that is Responsible for Multiple Gain of Function Skeletal Myopathy Mutations. Biophysical Journal. 2013; 104(2):646. View Publication
Orzechowski M, Fischer S, Lehman W. Influence of Actin Mutation on the Energy Landscape of Actin-Tropomyosin Filaments. Biophysical Journal. 2013; 104(2):480. View Publication
Cozhimuttam Viswanathan M, Lehman W, Cammarato A. A Troponin-T Mutation Initiates Cardiac and Skeletal Myopathy due to Impaired Inhibition of Contraction in Drosophila Melanogaster. Biophysical Journal. 2013; 104(2):311. View Publication
Yang S, Barbu-Tudoran L, Orzechowski M, Craig R, Trinick J, Lehman W, White H. 3D-Reconstruction Reveals the Organization of Troponin on Cardiac Thin Filaments. Biophysical Journal. 2013; 104(2):453.

Suphamungmee W, Nakamura F, Hartwig JH, Lehman W. Electron microscopy and 3D reconstruction reveals filamin Ig domain binding to F-actin. J Mol Biol. 2012 Dec 14; 424(5):248-56.View Related Profiles.

Lehman, William
Suphamungmee, Worawit

PMID: 23041423; PMCID: PMC3502691; DOI: 10.1016/j.jmb.2012.09.025;

Li XE, Suphamungmee W, Janco M, Geeves MA, Marston SB, Fischer S, Lehman W. The flexibility of two tropomyosin mutants, D175N and E180G, that cause hypertrophic cardiomyopathy. Biochem Biophys Res Commun. 2012 Aug 3; 424(3):493-6.View Related Profiles.

Lehman, William
Suphamungmee, Worawit
Li, Xiaochuan Li

PMID: 22789852; PMCID: PMC3412897; DOI: 10.1016/j.bbrc.2012.06.141;

Lehman W, Morgan KG. Structure and dynamics of the actin-based smooth muscle contractile and cytoskeletal apparatus. J Muscle Res Cell Motil. 2012 Dec; 33(6):461-9.View Related Profiles.

Morgan, Kathleen
Lehman, William

PMID: 22311558; PMCID: PMC3394904; DOI: 10.1007/s10974-012-9283-z;

Orzechowski M, Raunser S, Fischer S, Lehman W. Tropomyosin Movement on F-actin Analyzed by Energy Landscape Determination. Biophysical Journal. 2012; 102(3):17a. View Publication
Yang S, Barbu-Tudoran L, Craig R, Trinick J, Lehman W, White H. Visualization of Troponin on Muscle Thin Filaments by Single Particle Analysis. Biophysical Journal. 2012; 102(3):229. View Publication
Li X, Lehman W, Fischer S. Geometry of the C-/N-Terminal Connection of Tropomyosin on F-Actin Based on Molecular Dynamics Simulations of the Thin Filament. Biophysical Journal. 2012; 102(3):155. View Publication

Jensen MH, Watt J, Hodgkinson JL, Gallant C, Appel S, El-Mezgueldi M, Angelini TE, Morgan KG, Lehman W, Moore JR. Effects of basic calponin on the flexural mechanics and stability of F-actin. Cytoskeleton (Hoboken). 2012 Jan; 69(1):49-58.View Related Profiles.

Moore, Jeffrey
Morgan, Kathleen
Lehman, William

PMID: 22135101; PMCID: PMC3355516; DOI: 10.1002/cm.20548;

Rao JN, Rivera-Santiago R, Li XE, Lehman W, Dominguez R. Structural analysis of smooth muscle tropomyosin a and ß isoforms. J Biol Chem. 2012 Jan 27; 287(5):3165-74.View Related Profiles.

Lehman, William
Li, Xiaochuan Li

PMID: 22119916; PMCID: PMC3270971; DOI: 10.1074/jbc.M111.307330;

Cammarato A, Li XE, Reedy MC, Lee CF, Lehman W, Bernstein SI. Structural basis for myopathic defects engendered by alterations in the myosin rod. J Mol Biol. 2011 Dec 9; 414(4):477-84.View Related Profiles.

Lehman, William
Li, Xiaochuan Li

PMID: 22037585; PMCID: PMC3230674; DOI: 10.1016/j.jmb.2011.10.019;

Moore JR, Li X, Nirody J, Fischer S, Lehman W. Structural implications of conserved aspartate residues located in tropomyosin's coiled-coil core. Bioarchitecture. 2011 Sep 01; 1(5):250-255.View Related Profiles.

Moore, Jeffrey
Lehman, William

PMID: 22754618; PMCID: PMC3384579; DOI: 10.4161/bioa.18117;

East DA, Sousa D, Martin SR, Edwards TA, Lehman W, Mulvihill DP. Altering the stability of the Cdc8 overlap region modulates the ability of this tropomyosin to bind co-operatively to actin and regulate myosin. Biochem J. 2011 Sep 1; 438(2):265-73. PMID: 21658004; DOI: 10.1042/BJ20101316;

Mun JY, Gulick J, Robbins J, Woodhead J, Lehman W, Craig R. Electron microscopy and 3D reconstruction of F-actin decorated with cardiac myosin-binding protein C (cMyBP-C). J Mol Biol. 2011 Jul 8; 410(2):214-25. PMID: 21601575; PMCID: PMC3115431; DOI: 10.1016/j.jmb.2011.05.010;

Collins A, Huang R, Jensen MH, Moore JR, Lehman W, Wang CL. Structural studies on maturing actin filaments. Bioarchitecture. 2011 May; 1(3):127-133.View Related Profiles.

Moore, Jeffrey
Lehman, William

PMID: 21922043; PMCID: PMC3173961; DOI: 10.4161/bioa.1.3.16714;

Li XE, Tobacman LS, Mun JY, Craig R, Fischer S, Lehman W. Tropomyosin position on F-actin revealed by EM reconstruction and computational chemistry. Biophys J. 2011 Feb 16; 100(4):1005-13.View Related Profiles.

Lehman, William
Li, Xiaochuan Li

PMID: 21320445; PMCID: PMC3037716; DOI: 10.1016/j.bpj.2010.12.3697;

Gallant C, Appel S, Graceffa P, Leavis P, Lin JJ, Gunning PW, Schevzov G, Chaponnier C, DeGnore J, Lehman W, Morgan KG. Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells. Am J Physiol Cell Physiol. 2011 Jun; 300(6):C1356-65.View Related Profiles.

Morgan, Kathleen
Lehman, William

PMID: 21289288; PMCID: PMC3118631; DOI: 10.1152/ajpcell.00450.2010;

Li XE, Fischer S, Lehman W. Molecular Dynamics of Tropomyosin Bound to F-Actin. Biophysical Journal. 2011; 100(3). View Publication
Schmidt WM, Leavis PC, Lehman W, Moore JR. Monitoring the Real-Time Binding of Tropomyosin to Actin using Total Internal Reflection Fluorescence Microscopy. Biophysical Journal. 2011; 100(3). View Publication
Orzechowski M, Fischer S, Lehman W. Structural Simulations of Troponin-Regulated Tropomyosin Movement on F-Actin. Biophysical Journal. 2011; 100(3). View Publication
Li XE, Tobacman LS, Mun J, Craig R, Fischer S, Lehman W. Atomic Model of F-Actin-Tropomyosin. Biophysical Journal. 2011; 100(3). View Publication

Coulton AT, East DA, Galinska-Rakoczy A, Lehman W, Mulvihill DP. The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers permits the discrete regulation of specific myosins in fission yeast. J Cell Sci. 2010 Oct 1; 123(Pt 19):3235-43. PMID: 20807799; PMCID: PMC2939800; DOI: 10.1242/jcs.069971;

Sousa D, Cammarato A, Jang K, Graceffa P, Tobacman LS, Li XE, Lehman W. Electron microscopy and persistence length analysis of semi-rigid smooth muscle tropomyosin strands. Biophys J. 2010 Aug 4; 99(3):862-8.View Related Profiles.

Lehman, William
Li, Xiaochuan Li

PMID: 20682264; PMCID: PMC2913205; DOI: 10.1016/j.bpj.2010.05.004;

Li XE, Lehman W, Fischer S. The relationship between curvature, flexibility and persistence length in the tropomyosin coiled-coil. J Struct Biol. 2010 May; 170(2):313-8.View Related Profiles.

Lehman, William
Li, Xiaochuan Li

PMID: 20117217; PMCID: PMC2856770; DOI: 10.1016/j.jsb.2010.01.016;

Sousa D, Cammarato A, Li JP, Li X, Lehman W. Smooth Muscle Tropomyosin Forms Semi-Rigid End to End Polymers. Biophysical Journal. 2010; 3:98. View Publication
East DA, Sousa D, Lehman W, Mulvihill DP. Precise Modulation of Tropomyosin Polymer Length is Crucial for its Association with Actin and Ability to Regulate Myosin Function. Biophysical Journal. 2010; 98(3). View Publication
Cammarato A, Li X, Reedy MK, Lee C, Li P, Lehman W, Bernstein SI. Transgenic Replacement of the Myosin S2/hmm Hinge Alters the Rod's Nano-Mechanical Properties and Affects Sarcomeric Organization. Biophysical Journal. 2010; 98(3). View Publication
Jung HS, Sousa D, Tobacman LS, Craig R, Lehman W. Em and Single Particle Analysis of Troponin at Low and High Ca2+. Biophysical Journal. 2010; 98(3). View Publication

Galinska A, Hatch V, Craig R, Murphy AM, Van Eyk JE, Wang CL, Lehman W, Foster DB. The C terminus of cardiac troponin I stabilizes the Ca2+-activated state of tropomyosin on actin filaments. Circ Res. 2010 Mar 5; 106(4):705-11. PMID: 20035081; PMCID: PMC2834238; DOI: 10.1161/CIRCRESAHA.109.210047;

Li XE, Lehman W, Fischer S, Holmes KC. Curvature variation along the tropomyosin molecule. J Struct Biol. 2010 May; 170(2):307-12.View Related Profiles.

Lehman, William
Li, Xiaochuan Li

PMID: 20026408; PMCID: PMC2856783; DOI: 10.1016/j.jsb.2009.12.017;

Cammarato A, Craig R, Lehman W. Electron microscopy and three-dimensional reconstruction of native thin filaments reveal species-specific differences in regulatory strand densities. Biochem Biophys Res Commun. 2010 Jan 1; 391(1):193-7. PMID: 19900412; PMCID: PMC2818542; DOI: 10.1016/j.bbrc.2009.11.030;

Li XE, Holmes KC, Lehman W, Jung H, Fischer S. The shape and flexibility of tropomyosin coiled coils: implications for actin filament assembly and regulation. J Mol Biol. 2010 Jan 15; 395(2):327-39.View Related Profiles.

Lehman, William
Li, Xiaochuan Li

PMID: 19883661; DOI: 10.1016/j.jmb.2009.10.060;

Lehman W, Galinska-Rakoczy A, Hatch V, Tobacman LS, Craig R. Structural basis for the activation of muscle contraction by troponin and tropomyosin. J Mol Biol. 2009 May 15; 388(4):673-81. PMID: 19341744; PMCID: PMC2693027; DOI: 10.1016/j.jmb.2009.03.060;

Nirody J, Li X, Sousa D, Sumida J, Fischer S, Lehrer S, Lehman W. Electron Microscopy and Molecular Dynamics on a D137L Mutant of Tropomyosin. Biophysical Journal. 2009; 98(3):414. View Publication
Paul D, Lehman W, Pirani A, Craig R, Tobacman LS, Squire JM, Morris EP. Reference Free Single Particle Analysis Of Reconstituted Thin Filaments. Biophysical Journal. 2009; 96(3). View Publication
Facemyer KC, Dominguez R, Cremo CR, Lehman W. Computational Analysis of the Interaction Between Paired CH-Domains - Implications for their F-actin Bound Conformations. Biophysical Journal. 2009; 96(3). View Publication
Sousa D, Li X, Singh A, Jung H, Tobacman LS, Hitchcock-DeGregori SE, Lehman W. Tropomyosin Flexibility Evaluated by Electron Microscopy Image Analysis. Biophysical Journal. 2009; 96(3). View Publication
Li X, Lehman W, Holmes KC, Fischer S. Molecular Dynamics of Tropomyosin: Implications for the Assembly and Regulation of Thin Filaments. Biophysical Journal. 2009; 96(3). View Publication
Galinska-Rakoczy A, Hatch V, Craig R, Murphy AM, Van Eyk JE, Wang CLA, Lehman W, Foster DB. EM and 3D-Reconstruction of Thin Filaments Reconstituted with Truncated Troponin I Associated with Myocardial Stunning. Biophysical Journal. 2009; 96(3). View Publication

Holmes KC, Lehman W. Gestalt-binding of tropomyosin to actin filaments. J Muscle Res Cell Motil. 2008; 29(6-8):213-9. PMID: 19116763; DOI: 10.1007/s10974-008-9157-6;

Galinska-Rakoczy A, Engel P, Xu C, Jung H, Craig R, Tobacman LS, Lehman W. Structural basis for the regulation of muscle contraction by troponin and tropomyosin. J Mol Biol. 2008 Jun 20; 379(5):929-35. PMID: 18514658; PMCID: PMC2483953; DOI: 10.1016/j.jmb.2008.04.062;

Greenberg MJ, Wang CL, Lehman W, Moore JR. Modulation of actin mechanics by caldesmon and tropomyosin. Cell Motil Cytoskeleton. 2008 Feb; 65(2):156-64.View Related Profiles.

Moore, Jeffrey
Lehman, William

PMID: 18000881; PMCID: PMC2975105; DOI: 10.1002/cm.20251;

Lehman W, Craig R. Tropomyosin and the steric mechanism of muscle regulation. Adv Exp Med Biol. 2008; 644:95-109. PMID: 19209816

Maytum R, Hatch V, Konrad M, Lehman W, Geeves MA. Ultra short yeast tropomyosins show novel myosin regulation. J Biol Chem. 2008 Jan 25; 283(4):1902-10. PMID: 18006493

Skoumpla K, Coulton AT, Lehman W, Geeves MA, Mulvihill DP. Acetylation regulates tropomyosin function in the fission yeast Schizosaccharomyces pombe. J Cell Sci. 2007 May 1; 120(Pt 9):1635-45. PMID: 17452625

Poole KJ, Lorenz M, Evans G, Rosenbaum G, Pirani A, Craig R, Tobacman LS, Lehman W, Holmes KC. A comparison of muscle thin filament models obtained from electron microscopy reconstructions and low-angle X-ray fibre diagrams from non-overlap muscle. J Struct Biol. 2006 Aug; 155(2):273-84. PMID: 16793285

Pant K, Chereau D, Hatch V, Dominguez R, Lehman W. Cortactin binding to F-actin revealed by electron microscopy and 3D reconstruction. J Mol Biol. 2006 Jun 16; 359(4):840-7. PMID: 16697006

Pirani A, Vinogradova MV, Curmi PM, King WA, Fletterick RJ, Craig R, Tobacman LS, Xu C, Hatch V, Lehman W. An atomic model of the thin filament in the relaxed and Ca2+-activated states. J Mol Biol. 2006 Mar 31; 357(3):707-17. PMID: 16469331

Cammarato A, Craig R, Sparrow JC, Lehman W. E93K charge reversal on actin perturbs steric regulation of thin filaments. J Mol Biol. 2005 Apr 15; 347(5):889-94. PMID: 15784249

Pirani A, Xu C, Hatch V, Craig R, Tobacman LS, Lehman W. Single particle analysis of relaxed and activated muscle thin filaments. J Mol Biol. 2005 Feb 25; 346(3):761-72. PMID: 15713461

Gong H, Hatch V, Ali L, Lehman W, Craig R, Tobacman LS. Mini-thin filaments regulated by troponin-tropomyosin. Proc Natl Acad Sci U S A. 2005 Jan 18; 102(3):656-61. PMID: 15644437; PMCID: PMC545539; DOI: 10.1073/pnas.0407225102;

Pirani A, Hatch V, Curmi P, King B, Vinogradova M, Fletterick R, Craig R, Tobacman LS, Xu C, Lehman W. Biophysical Journal. Defining the positions of troponin and tropomyosin on thin filaments by EM and single particle analysis. 2005.

Foster DB, Huang R, Hatch V, Craig R, Graceffa P, Lehman W, Wang CL. Modes of caldesmon binding to actin: sites of caldesmon contact and modulation of interactions by phosphorylation. J Biol Chem. 2004 Dec 17; 279(51):53387-94. PMID: 15456752

Cammarato A, Hatch V, Saide J, Craig R, Sparrow JC, Tobacman LS, Lehman W. Drosophila muscle regulation characterized by electron microscopy and three-dimensional reconstruction of thin filament mutants. Biophys J. 2004 Mar; 86(3):1618-24.View Related Profiles.

Saide, Judith
Lehman, William

PMID: 14990488; PMCID: PMC1303996

Lehman W, Craig R, Tobacman LS, Pirani A, Hatch V, Xu C. Molecular Biology of the Cell. Defining the positions of tropomyosin and troponin on thin filaments. 2004.

Lehman W, Craig R. The structure of the vertebrate striated muscle thin filament: a tribute to the contributions of Jean Hanson. J Muscle Res Cell Motil. 2004; 25(6):455-66. PMID: 15630610

Lehman W, Craig R, Tobaman LS, Xu C. Biophysical Journal. Defining the positions of tropomyosin on thin filaments in the relaxed-state by EM and 3D reconstruction. 2004.

Lehman W, Craig R, Kendrick-Jones J, Sutherland-Smith AJ. An open or closed case for the conformation of calponin homology domains on F-actin? J Muscle Res Cell Motil. 2004; 25(4-5):351-8. PMID: 15548864

Sutherland-Smith AJ, Moores CA, Norwood FL, Hatch V, Craig R, Kendrick-Jones J, Lehman W. An atomic model for actin binding by the CH domains and spectrin-repeat modules of utrophin and dystrophin. J Mol Biol. 2003 May 23; 329(1):15-33. PMID: 12742015

Tobacman LS, Nihli M, Butters C, Heller M, Hatch V, Craig R, Lehman W, Homsher E. The troponin tail domain promotes a conformational state of the thin filament that suppresses myosin activity. J Biol Chem. 2002 Aug 2; 277(31):27636-42. PMID: 12011043

Craig R, Lehman W. The ultrastructural basis of actin filament regulation. Results Probl Cell Differ. 2002; 36:149-69. PMID: 11892278

Lehman W, Hatch V, Craig R, Kendrick-Jones J, Sutherland-Smith AJ. 3-D imaging of dystrophin and utrophin on F-actin. Biophysical Journal. 2002; 82(1):6A.

Craig R, Lehman W. Crossbridge and tropomyosin positions observed in native, interacting thick and thin filaments. J Mol Biol. 2001 Aug 31; 311(5):1027-36. PMID: 11531337

Hatch V, Zhi G, Smith L, Stull JT, Craig R, Lehman W. Myosin light chain kinase binding to a unique site on F-actin revealed by three-dimensional image reconstruction. J Cell Biol. 2001 Aug 6; 154(3):611-7. PMID: 11481347; PMCID: PMC2196421

Lehman W, Rosol M, Tobacman LS, Craig R. Troponin organization on relaxed and activated thin filaments revealed by electron microscopy and three-dimensional reconstruction. J Mol Biol. 2001 Mar 30; 307(3):739-44. PMID: 11273697

Burhop J, Rosol M, Craig R, Tobacman LS, Lehman W. Effects of a cardiomyopathy-causing troponin t mutation on thin filament function and structure. J Biol Chem. 2001 Jun 8; 276(23):20788-94. PMID: 11262409

Lehman W, Hatch V, Korman V, Rosol M, Thomas L, Maytum R, Geeves MA, Van Eyk JE, Tobacman LS, Craig R. Tropomyosin and actin isoforms modulate the localization of tropomyosin strands on actin filaments. J Mol Biol. 2000 Sep 22; 302(3):593-606. PMID: 10986121

Korman VL, Hatch V, Dixon KY, Craig R, Lehman W, Tobacman LS. An actin subdomain 2 mutation that impairs thin filament regulation by troponin and tropomyosin. J Biol Chem. 2000 Jul 21; 275(29):22470-8. PMID: 10801864

Rosol M, Lehman W, Craig R, Landis C, Butters C, Tobacman LS. Three-dimensional reconstruction of thin filaments containing mutant tropomyosin. Biophys J. 2000 Feb; 78(2):908-17. PMID: 10653803; PMCID: PMC1300693

Xu C, Craig R, Tobacman L, Horowitz R, Lehman W. Tropomyosin positions in regulated thin filaments revealed by cryoelectron microscopy. Biophys J. 1999 Aug; 77(2):985-92. PMID: 10423443; PMCID: PMC1300389

Hanein D, Volkmann N, Goldsmith S, Michon AM, Lehman W, Craig R, DeRosier D, Almo S, Matsudaira P. An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation. Nat Struct Biol. 1998 Sep; 5(9):787-92. PMID: 9731773

Lehman W, Vibert P, Craig R. Visualization of caldesmon on smooth muscle thin filaments. J Mol Biol. 1997 Dec 5; 274(3):310-7. PMID: 9405141

Hodgkinson JL, el-Mezgueldi M, Craig R, Vibert P, Marston SB, Lehman W. 3-D image reconstruction of reconstituted smooth muscle thin filaments containing calponin: visualization of interactions between F-actin and calponin. J Mol Biol. 1997 Oct 17; 273(1):150-9. PMID: 9367753

Hodgkinson JL, Marston SB, Craig R, Vibert P, Lehman W. Three-dimensional image reconstruction of reconstituted smooth muscle thin filaments: effects of caldesmon. Biophys J. 1997 Jun; 72(6):2398-404. PMID: 9168017; PMCID: PMC1184439

Vibert P, Craig R, Lehman W. Steric-model for activation of muscle thin filaments. J Mol Biol. 1997 Feb 14; 266(1):8-14. PMID: 9054965

Lehman W., R. Craig, & M. Barany . Actin and the Structure of Smooth Muscle Thin Filaments. In Biochemistry of Smooth Muscle Contraction, Barany, M. (editor). Academic Press. San Diego. 1996; 47-60.
Lehman W, Craig R, Uman P, Vibert P. Tropomyosin movement in thin filaments following Ca2+-activation. Biophysical Journal. 1996; 70(2):SUA18.
Vibert P, Craig R, Uman P, Lehman W. Towards an atomic model for regulation by tropomyosin troponin. Biophysical Journal. 1996; 70(2).

Lehman W, Vibert P, Uman P, Craig R. Steric-blocking by tropomyosin visualized in relaxed vertebrate muscle thin filaments. J Mol Biol. 1995 Aug 11; 251(2):191-6. PMID: 7643394

Lehman W, Craig R, Vibert P. Ca(2+)-induced tropomyosin movement in Limulus thin filaments revealed by three-dimensional reconstruction. Nature. 1994 Mar 3; 368(6466):65-7. PMID: 8107884

Lehman W, Denault D, Marston S. The caldesmon content of vertebrate smooth muscle. Biochim Biophys Acta. 1993 Nov 10; 1203(1):53-9. PMID: 8218392

Vibert P, Craig R, Lehman W. Three-dimensional reconstruction of caldesmon-containing smooth muscle thin filaments. J Cell Biol. 1993 Oct; 123(2):313-21. PMID: 8408215; PMCID: PMC2119844

Graceffa P, Adam LP, Lehman W. Disulphide cross-linking of smooth-muscle and non-muscle caldesmon to the C-terminus of actin in reconstituted and native thin filaments. Biochem J. 1993 Aug 15; 294 ( Pt 1):63-7. PMID: 8363587; PMCID: PMC1134566

Lehman W, Denault D, Marston S. Novel immunological technique. J Muscle Res Cell Motil. 1992 Oct; 13(5):582-5. PMID: 1460085

Lehman W, Denault D, Marston S. Correspondence. Journal of Muscle Research and Cell Motility. 1992; 13(5). View Publication

Lehman W. Calponin and the composition of smooth muscle thin filaments. J Muscle Res Cell Motil. 1991 Jun; 12(3):221-4. PMID: 1874963

Moody C, Lehman W, Craig R. Caldesmon and the structure of smooth muscle thin filaments: electron microscopy of isolated thin filaments. J Muscle Res Cell Motil. 1990 Apr; 11(2):176-85. PMID: 2351755

Lehman W, Moody C, Craig R. Caldesmon and the structure of vertebrate smooth muscle thin filaments. A minireview. Ann N Y Acad Sci. 1990; 599:75-84. PMID: 2221678

Babu A, Lehman W, Gulati J. Characterization of the Ca2+-switch in skeletal and cardiac muscles. FEBS Lett. 1989 Jul 17; 251(1-2):177-82. PMID: 2753157

Lehman W. 35 kDa proteins are not components of vertebrate smooth muscle thin filaments. Biochim Biophys Acta. 1989 Jun 13; 996(1-2):57-61. PMID: 2736259

Lehman W, Craig R, Lui J, Moody C. Caldesmon and the structure of smooth muscle thin filaments: immunolocalization of caldesmon on thin filaments. J Muscle Res Cell Motil. 1989 Apr; 10(2):101-12. PMID: 2760189

Marston SB, Redwood CS, Lehman W. Reversal of caldesmon function by anti-caldesmon antibodies confirms its role in the calcium regulation of vascular smooth muscle thin filaments. Biochem Biophys Res Commun. 1988 Aug 30; 155(1):197-202. PMID: 2970847

Marston S, Lehman W, Woody C, Pritchard K, Smith CWJ. Caldesmon and Ca2+ Regulation in Smooth Muscles. Calcium Binding Proteins. 1988. View Publication

Lehman W, Sheldon A, Madonia W. Diversity in smooth muscle thin filament composition. Biochim Biophys Acta. 1987 Jul 24; 914(1):35-9. PMID: 3607060

Lehman W. The effect of calcium on the aggregation of chicken gizzard thin filaments. J Muscle Res Cell Motil. 1986 Dec; 7(6):537-49. PMID: 3100573

Lehman W. Caldesmon association with smooth muscle thin filaments isolated in the presence and absence of calcium. Biochim Biophys Acta. 1986 Jan 23; 885(1):88-90. PMID: 3942796

Marston SB, Lehman W. Caldesmon is a Ca2+-regulatory component of native smooth-muscle thin filaments. Biochem J. 1985 Nov 1; 231(3):517-22. PMID: 2934055; PMCID: PMC1152781

Shima Y, Tsuchiya T, Lehman W, Matsumoto JJ. The characterization of invertebrate troponin C. Comp Biochem Physiol B. 1984; 79(4):525-9. PMID: 6518756

Lehman W. The distribution of troponin-like proteins on thin filaments of the bay scallop, aequipecten irradians. J Muscle Res Cell Motil. 1983 Jun; 4(3):379-89. PMID: 6348084

Lehman W. The ionic requirements for regulation by molluscan thin filaments. Biochim Biophys Acta. 1983 May 30; 745(1):1-5. PMID: 6221762

Lehman W. The location and periodicity of a troponin-T-like protein in the myofibril of the horseshoe crab Limulus polyphemus. J Mol Biol. 1982 Jan 15; 154(2):385-91. PMID: 6804635

Tsuchiya T, Head JF, Lehman W. The isolation and characterization of a troponin-C-like protein from the mantle muscle of the squid Loligo pealei. Comparative biochemistry and physiology. B, Comparative biochemistry. 1982; 71(3):507-509. View Publication

Lehman W. Thin-filament-linked regulation in molluscan muscles. Biochim Biophys Acta. 1981 May 29; 668(3):349-56. PMID: 6453619

Lehman W, Ferrell M. Phylogenetic diversity of troponin subunit-C amino acid composition. FEBS Lett. 1980 Dec 1; 121(2):273-4. PMID: 7461130

Lehman W, Head JF, Grant PW. The stoichiometry and location of troponin I- and troponin C-like proteins in the myofibril of the bay scallop, Aequipecten irradians. Biochem J. 1980 May 1; 187(2):447-56.View Related Profiles.

Head, James
Lehman, William

PMID: 6249269; PMCID: PMC1161811

Lehman W. Thick-filament-linked calcium regulation in vertebrate striated muscle. Nature. 1978 Jul 6; 274(5666):80-1. PMID: 149254

Goldberg A, Lehman W. Troponin-like proteins from muscles of the scallop, Aequipecten irradians. Biochem J. 1978 May 1; 171(2):413-8. PMID: 148888; PMCID: PMC1183970

Lehman W. Calcium ion-dependent myosin from decapod-crustacean muscles. Biochem J. 1977 May 1; 163(2):291-6. PMID: 141278; PMCID: PMC1164695

Lehman W. Insect Flight Muscle: Proceedings of the Oxford Symposium. The dual system of calcium-activation in arthropod muscles. North Holland. Amsterdam. 1977; 277-283.

Lehman W, Regenstein JM, Ransom AL. The stoichiometry of the components of arthropod thin filaments. Biochim Biophys Acta. 1976 May 20; 434(1):215-22. PMID: 938665

Lehman W. Phylogenetic diversity of the proteins regulating muscular contraction. Int Rev Cytol. 1976; 44:55-92. PMID: 131113

Lehman W. Cell Motility, Book A, Motility, Muscle, and Non-muscle Cells. Calcium regulation in invertebrate muscles. Cold Spring Harbor Press. Woodbury, NY. 1976; 3A:277-283.

Fine R, Lehman W, Head J, Blitz A. Troponin C in brain. Nature. 1975 Nov 20; 258(5532):260-7. PMID: 1202358

Lehman W, Szent-Györgyi AG. Regulation of muscular contraction. Distribution of actin control and myosin control in the animal kingdom. J Gen Physiol. 1975 Jul; 66(1):1-30. PMID: 125778; PMCID: PMC2226187

Lehman W. Hybrid troponin reconstituted from vertebrate and arthropod subunits. Nature. 1975 May 29; 255(5507):424-6. PMID: 124018

Marston S, Lehman W. ADP binding to relaxed scallop myofibrils. Nature. 1974 Nov 1; 252(5478):38-9. PMID: 4279338

Lehman W, Bullard B, Hammond K. Calcium-dependent myosin from insect flight muscles. J Gen Physiol. 1974 May; 63(5):553-63. PMID: 4274833; PMCID: PMC2203566

Lehman W, Kendrick-Jones J, Szent-Gyorgyi AG. Myosin-linked Regulatory Systems: Comparative Studies. Cold Spring Harbor Symposia on Quantitative Biology. 1973; 37:319-330. View Publication
Lehman W., J. Kendrick Jones, & A.G. Szent Györgyi . Cold Spring Harbor Symposium: The Mechanism of Muscle Contraction. Myosin linked Regulatory Systems: Comparative Aspects. Cold Spring Harbor Laboratory Press. Woodbury, NY. 1973; 37:319-330.

Lehman W, Szent-Györgyi G. Activation of the adenosine triphosphatase of Limulus polyphemus actomyosin by tropomyosin. J Gen Physiol. 1972 Apr; 59(4):375-87. PMID: 4260494; PMCID: PMC2203184

Lehman WJ, Rebhun LI. The structural elements responsible for contraction in the ciliate Spirostomum. Protoplasma. 1971; 72(2):153-78. PMID: 5555985

Kendrick-Jones J, Lehman W, Szent-Györgyi AG. Regulation in molluscan muscles. J Mol Biol. 1970 Dec 14; 54(2):313-26. PMID: 4250215

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 165 publications over 43 distinct years, with a maximum of 13 publications in 2011

Bar chart showing 165 publications over 43 distinct years, with a maximum of 13 publications in 2011

Year	Publications
1980	2
1981	1
1982	2
1983	2
1984	1
1985	1
1986	2
1987	1
1988	2
1989	3
1990	2
1991	1
1992	2
1993	3
1994	1
1995	1
1996	3
1997	4
1998	1
1999	1
2000	3
2001	4
2002	3
2003	1
2004	6
2005	4
2006	3
2007	2
2008	4
2009	11
2010	7
2011	13
2012	6
2013	11
2014	9
2015	6
2016	9
2017	7
2018	6
2019	5
2020	3
2021	2
2022	4