Matthew D. Layne, PhD
Associate Professor
Boston University School of Medicine
Dept of Biochemistry

PhD, Boston University School of Medicine

The primary goal of our laboratory is to identify novel pathways that control extracellular matrix (ECM) synthesis and assembly as they relate to fibroproliferative and connective tissue diseases. Our long term goal is to use this knowledge to develop therapeutic strategies for these conditions. Fibroproliferative responses are similar to wound healing processes involving accumulation of contractile myofibroblasts and ECM secretion and assembly. Because organ fibrosis, cardiovascular, metabolic/obesity, and cancer pathologies are now recognized to be impacted by fibroblast-myofibroblast differentiation and ECM remodeling our research is examining novel pathways and control mechanisms in these diseases. In collaborative work, we are investigating the mechanisms of adipose tissue fibrosis and remodeling. Central to our studies is determining the function of Aortic Carboxypeptidase-like Protein (ACLP), a secreted, collagen-binding protein that enhances fibrosis and myofibroblast differentiation through mechanisms that involve stimulating the transforming growth factor ß (TGFß) receptor signaling complex and controlling mechanical signaling and ECM remodeling. Recent work is uncovering the role of ACLP (and AEBP1 genetic mutations in the connective tissue disease Ehlers-Danlos syndrome. There are several active projects in the lab including:

• Investigating the mechanisms of how ACLP/AEBP1 mutations cause Ehlers-Danlos syndrome (EDS)
• Defining the the role of ACLP in mechanotransduction pathways that control progenitor differentiation.
• Developing strategies to organ fibrosis through targeting ACLP
• Uncovering new mechanisms that control adipose tissue fibrosis.

Boston University
BU-BMC Cancer Center

Boston University
Evans Center for Interdisciplinary Biomedical Research

Graduate Faculty (Primary Mentor of Grad Students)
Boston University School of Medicine, Graduate Medical Sciences

Member of the Cell and Molecular Biology Program
Boston University School of Medicine

Member of the Molecular Medicine Program
Boston University School of Medicine

Boston University
Genome Science Institute

2011 Fellow of the American Heart Association

Generation of a novel transgenic mouse line for studying adipose tissue fibrosis
10/01/2018 - 05/31/2020 (Subcontract PI)
Boston Medical Center Corporation NIH NIDDK

Development of Tumor-Directed Therapies
05/01/2015 - 04/30/2019 (PI)
Department of Defense/Army Medical Research Acquisition Activity

Role of ACLP in adipocyte determination and fibrosis
01/21/2016 - 03/31/2017 (Subcontract PI)
Boston Medical Center Corporation NIH NIDDK

ACLP-mediated Vascular Advential Fibroblast Differentiation and Remodeling
01/01/2014 - 12/31/2016 (PI)
American Heart Association

Inhibitors of Aortic Carboxypeptides-like Proten to Treat Fibroproliferative Diseases
01/01/2015 - 07/02/2016 (PI)
Pfizer, Inc.

Role of ACLP in adipocyte determination and fibrosis
04/01/2012 - 03/31/2014 (Subcontract PI)
Boston Medical Center Corporation NIH NIDDK


Yr Title Project-Sub Proj Pubs
2011 Role of ACLP in Vascular Smooth Muscle Biology 5R01HL078869-06 12
2010 Role of ACLP in Vascular Smooth Muscle Biology 5R01HL078869-05 12
2009 Role of ACLP in Vascular Smooth Muscle Biology 3R01HL078869-04S1 12
2009 Role of ACLP in Vascular Smooth Muscle Biology 5R01HL078869-04 12
2008 Role of ACLP in Vascular Smooth Muscle Biology 5R01HL078869-03 12
2007 Role of ACLP in Vascular Smooth Muscle Biology 1R01HL078869-01A2 12
2007 Role of ACLP in Vascular Smooth Muscle Biology 7R01HL078869-02 12
2004 Role of ACLP in dermal wound healing 5K01AR047861-04 4
2003 Role of ACLP in dermal wound healing 5K01AR047861-03 4
2002 Role of ACLP in dermal wound healing 5K01AR047861-02 4
Showing 10 of 13 results. Show All Results

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

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  1. Zeng C, Motta-Ribeiro GC, Hinoshita T, Lessa MA, Winkler T, Grogg K, Kingston NM, Hutchinson JN, Sholl LM, Fang X, Varelas X, Layne MD, Baron RM, Vidal Melo MF. Lung Atelectasis Promotes Immune and Barrier Dysfunction as Revealed by Transcriptome Sequencing in Female Sheep. Anesthesiology. 2020 11 01; 133(5):1060-1076.View Related Profiles. PMID: 32796202
  2. Vishwanath N, Monis WJ, Hoffmann GA, Ramachandran B, DiGiacomo V, Wong JY, Smith ML, Layne MD. Mechanisms of aortic carboxypeptidase-like protein secretion and identification of an intracellularly retained variant associated with Ehlers-Danlos syndrome. J Biol Chem. 2020 Jul 10; 295(28):9725-9735.View Related Profiles. PMID: 32482891
  3. Lee MJ, Pickering RT, Shibad V, Wu Y, Karastergiou K, Jager M, Layne MD, Fried SK. Impaired Glucocorticoid Suppression of TGFß Signaling in Human Omental Adipose Tissues Limits Adipogenesis and May Promote Fibrosis. Diabetes. 2019 03; 68(3):587-597.View Related Profiles. PMID: 30530781
  4. Baron RM, Kwon MY, Castano AP, Ghanta S, Riascos-Bernal DF, Lopez-Guzman S, Macias AA, Ith B, Schissel SL, Lederer JA, Reeves R, Yet SF, Layne MD, Liu X, Perrella MA. Frontline Science: Targeted expression of a dominant-negative high mobility group A1 transgene improves outcome in sepsis. J Leukoc Biol. 2018 10; 104(4):677-689. PMID: 29975792
  5. Jager M, Lee MJ, Li C, Farmer SR, Fried SK, Layne MD. Aortic carboxypeptidase-like protein enhances adipose tissue stromal progenitor differentiation into myofibroblasts and is upregulated in fibrotic white adipose tissue. PLoS One. 2018; 13(5):e0197777.View Related Profiles. PMID: 29799877
  6. Blackburn PR, Xu Z, Tumelty KE, Zhao RW, Monis WJ, Harris KG, Gass JM, Cousin MA, Boczek NJ, Mitkov MV, Cappel MA, Francomano CA, Parisi JE, Klee EW, Faqeih E, Alkuraya FS, Layne MD, McDonnell NB, Atwal PS. Bi-allelic Alterations in AEBP1 Lead to Defective Collagen Assembly and Connective Tissue Structure Resulting in a Variant of Ehlers-Danlos Syndrome. Am J Hum Genet. 2018 04 05; 102(4):696-705.View Related Profiles. PMID: 29606302
  7. Shiwen X, Stratton R, Nikitorowicz-Buniak J, Ahmed-Abdi B, Ponticos M, Denton C, Abraham D, Takahashi A, Suki B, Layne MD, Lafyatis R, Smith BD. A Role of Myocardin Related Transcription Factor-A (MRTF-A) in Scleroderma Related Fibrosis. PLoS One. 2015; 10(5):e0126015.View Related Profiles. PMID: 25955164; PMCID: PMC4425676; DOI: 10.1371/journal.pone.0126015;
  8. McDonald ME, Li C, Bian H, Smith BD, Layne MD, Farmer SR. Myocardin-related transcription factor A regulates conversion of progenitors to beige adipocytes. Cell. 2015 Jan 15; 160(1-2):105-18.View Related Profiles. PMID: 25579684; PMCID: PMC4384505; DOI: 10.1016/j.cell.2014.12.005;
  9. Xu YX, Ashline D, Liu L, Tassa C, Shaw SY, Ravid K, Layne MD, Reinhold V, Robbins PW. The glycosylation-dependent interaction of perlecan core protein with LDL: implications for atherosclerosis. J Lipid Res. 2015 Feb; 56(2):266-76.View Related Profiles. PMID: 25528754; PMCID: PMC4306681; DOI: 10.1194/jlr.M053017;
  10. Chen CH, Ho HH, Wu ML, Layne MD, Yet SF. Modulation of cysteine-rich protein 2 expression in vascular injury and atherosclerosis. Mol Biol Rep. 2014 Nov; 41(11):7033-41. PMID: 25034893; DOI: 10.1007/s11033-014-3591-x;
Showing 10 of 73 results. Show More

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 73 publications over 24 distinct years, with a maximum of 9 publications in 2001


In addition to these self-described keywords below, a list of MeSH based concepts is available here.

Extracellular Matrix Proteins, Ehlers-Danlos syndrome, fibrosis, mechanostransduction, collagen

My research interests include mechanisms of smooth muscle changes in vascular disease, fibrosis, and now cancer. In a research or laboratory setting I have mentored numerous scientists at the undergraduate, graduate, and postdoctoral levels and have participated on more than 25 PhD dissertation committees.

Currently I serve as an academic advisor in the Master's in Medical Sciences (MAMS) program.

Available to Mentor as: (Review Mentor Role Definitions):
  • Advisor
  • Education Mentor
  • Project Mentor
  • Research / Scholarly Mentor
Contact for Mentoring:
  • Email (see 'Contact Info')

72 E. Concord St Silvio Conte (K)
Boston MA 02118
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