Matthew Layne | Profiles RNS

Matthew D. Layne, PhD

Associate Professor

Boston University Chobanian & Avedisian School of Medicine

Biochemistry & Cell Biology

PhD, Boston University School of Medicine
BA, Boston University

Pronouns: he/him/his

Websites

	Biochemistry Faculty
	Medical Student Research

ORCID : 0000-0003-0007-4870

The primary goal of our laboratory is to identify novel pathways that control extracellular matrix (ECM) synthesis and assembly as they relate to fibroproliferative and connective tissue diseases. Our long term goal is to use this knowledge to develop therapeutic strategies for these conditions. Fibroproliferative responses are similar to wound healing processes involving accumulation of contractile myofibroblasts and ECM secretion and assembly. Because organ fibrosis, cardiovascular, metabolic/obesity, and cancer pathologies are now recognized to be impacted by fibroblast-myofibroblast differentiation and ECM remodeling our research is examining novel pathways and control mechanisms in these diseases. In collaborative work, we are investigating the mechanisms of adipose tissue fibrosis and remodeling. Central to our studies is determining the function of Aortic Carboxypeptidase-like Protein (ACLP), a secreted, collagen-binding protein that enhances fibrosis and myofibroblast differentiation through mechanisms that involve stimulating the transforming growth factor ß (TGFß) receptor signaling complex and controlling mechanical signaling and ECM remodeling. Recent work is uncovering the role of ACLP (and AEBP1 genetic mutations in the connective tissue disease Ehlers-Danlos syndrome. There are several active projects in the lab including:

• Investigating the mechanisms of how ACLP/AEBP1 mutations cause Ehlers-Danlos syndrome (EDS)
• Defining the the role of ACLP in mechanotransduction pathways that control progenitor differentiation.
• Developing strategies to organ fibrosis through targeting ACLP
• Uncovering new mechanisms that control adipose tissue fibrosis.

Assistant Dean of Research
Boston University Chobanian & Avedisian School of Medicine

Member
Boston University
BU-BMC Cancer Center

Member
Boston University
Evans Center for Interdisciplinary Biomedical Research

Graduate Faculty (Primary Mentor of Grad Students)
Boston University Chobanian & Avedisian School of Medicine, Graduate Medical Sciences

Member of the Molecular Medicine Program
Boston University Chobanian & Avedisian School of Medicine

Member
Boston University
Genome Science Institute

BU Research
NIH RePORTER Research

Deconstructing the diet-induced remodeling of adipose tissue
03/01/2023 - 02/28/2027 (Multi-PI)
PI: Matthew D. Layne, PhD
NIH/National Diabetes & Digestive & Kidney Diseases
1R01DK134534-01

Elucidating ACLP-dependent signaling pathways in the adipose tissue stromal-vascular niche
04/15/2022 - 03/31/2026 (PI)
NIH/National Diabetes & Digestive & Kidney Diseases
5R01DK132080-02

Optical biosensor platforms for the direct interrogation of GPCR signaling in cardiovascular cells
01/01/2022 - 12/31/2023 (Key Person / Mentor)
American Heart Association

Generation of a novel transgenic mouse line for studying adipose tissue fibrosis
10/01/2018 - 05/31/2020 (Subcontract PI)
Boston Medical Center Corporation NIH NIDDK
3P30DK046200-26S2

Development of Tumor-Directed Therapies
05/01/2015 - 04/30/2019 (PI)
Department of Defense/Army Medical Research Acquisition Activity

Role of ACLP in adipocyte determination and fibrosis
01/21/2016 - 03/31/2017 (Subcontract PI)
Boston Medical Center Corporation NIH NIDDK
4P30DK046200-24

ACLP-mediated Vascular Advential Fibroblast Differentiation and Remodeling
01/01/2014 - 12/31/2016 (PI)
American Heart Association

Inhibitors of Aortic Carboxypeptides-like Proten to Treat Fibroproliferative Diseases
01/01/2015 - 07/02/2016 (PI)
Pfizer, Inc.

Enhancing Energy Expending Adipocytes in White Adipose Tissue
04/01/2013 - 03/31/2016 (Key Person)
PI: Stephen Farmer, PhD
NIH/National Diabetes & Digestive & Kidney Diseases
4R01DK098830-04

Role of ACLP in adipocyte determination and fibrosis
04/01/2012 - 03/31/2014 (Subcontract PI)
Boston Medical Center Corporation NIH NIDDK
5P30DK046200-20

Title

Yr	Title	Project-Sub Proj	Pubs
2024	Deconstructing the diet-induced remodeling of adipose tissue	5R01DK134534-02
2023	Deconstructing the diet-induced remodeling of adipose tissue	1R01DK134534-01
2023	Elucidating ACLP-dependent signaling pathways in the adipose tissue stromal-vascular niche	5R01DK132080-02
2022	Elucidating ACLP-dependent signaling pathways in the adipose tissue stromal-vascular niche	1R01DK132080-01
2011	Role of ACLP in Vascular Smooth Muscle Biology	5R01HL078869-06	12
2010	Role of ACLP in Vascular Smooth Muscle Biology	5R01HL078869-05	12
2009	Role of ACLP in Vascular Smooth Muscle Biology	3R01HL078869-04S1	12
2009	Role of ACLP in Vascular Smooth Muscle Biology	5R01HL078869-04	12
2008	Role of ACLP in Vascular Smooth Muscle Biology	5R01HL078869-03	12
2007	Role of ACLP in Vascular Smooth Muscle Biology	1R01HL078869-01A2	12

Showing 10 of 17 results. Show All Results

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis Copy PMIDs To Clipboard

Lin W, Mousavi F, Blum BC, Heckendorf CF, Moore J, Lampl N, McComb M, Kotelnikov S, Yin W, Rabhi N, Layne MD, Kozakov D, Chitalia VC, Emili A. Corrigendum: Integrated metabolomics and proteomics reveal biomarkers associated with hemodialysis in end-stage kidney disease. Front Pharmacol. 2024; 15:1376058.View Related Profiles.

Blum, Benjamin
Layne, Matthew
Rabhi, Nabil
Chitalia, Vipul

PMID: 38405670; PMCID: PMC10884821; DOI: 10.3389/fphar.2024.1376058;

Lin W, Mousavi F, Blum BC, Heckendorf CF, Moore J, Lampl N, McComb M, Kotelnikov S, Yin W, Rabhi N, Layne MD, Kozakov D, Chitalia VC, Emili A. Integrated metabolomics and proteomics reveal biomarkers associated with hemodialysis in end-stage kidney disease. Front Pharmacol. 2023; 14:1243505.View Related Profiles.

Blum, Benjamin
Layne, Matthew
Rabhi, Nabil
Chitalia, Vipul
Lin, Weiwei

PMID: 38089059; PMCID: PMC10715419; DOI: 10.3389/fphar.2023.1243505;

Desevin K, Cortez BN, Lin JZ, Lama D, Layne MD, Farmer SR, Rabhi N. Adrenergic Reprogramming of Preexisting Adipogenic Trajectories Steer Naïve Mural Cells Toward Beige Differentiation. bioRxiv. 2023 Aug 26.View Related Profiles.

Layne, Matthew
Rabhi, Nabil
Farmer, Stephen

PMID: 37662295; PMCID: PMC10473761; DOI: 10.1101/2023.08.26.554950;

Dance YW, Obenreder MC, Seibel AJ, Meshulam T, Ogony JW, Lahiri N, Pacheco-Spann L, Radisky DC, Layne MD, Farmer SR, Nelson CM, Tien J. Adipose Cells Induce Escape from an Engineered Human Breast Microtumor Independently of their Obesity Status. Cell Mol Bioeng. 2023 Feb; 16(1):23-39.View Related Profiles.

Tien, Joe
Layne, Matthew
Farmer, Stephen
Meshulam, Tova

PMID: 36660589; PMCID: PMC9842842; DOI: 10.1007/s12195-022-00750-y;

Chen CH, Ho HH, Jiang WC, Ao-Ieong WS, Wang J, Orekhov AN, Sobenin IA, Layne MD, Yet SF. Cysteine-rich protein 2 deficiency attenuates angiotensin II-induced abdominal aortic aneurysm formation in mice. J Biomed Sci. 2022 Apr 12; 29(1):25. PMID: 35414069; PMCID: PMC9004090; DOI: 10.1186/s12929-022-00808-z;

Rabhi N, Desevin K, Belkina AC, Tilston-Lunel A, Varelas X, Layne MD, Farmer SR. Obesity-induced senescent macrophages activate a fibrotic transcriptional program in adipocyte progenitors. Life Sci Alliance. 2022 05; 5(5).View Related Profiles.

Tilston-Lunel, Andrew
Belkina, Anna
Layne, Matthew
Rabhi, Nabil
Farmer, Stephen
Varelas, Xaralabos

PMID: 35181634; PMCID: PMC8860101; DOI: 10.26508/lsa.202101286;

Downs M, Sethi MK, Raghunathan R, Layne MD, Zaia J. Matrisome changes in Parkinson's disease. Anal Bioanal Chem. 2022 Apr; 414(9):3005-3015.View Related Profiles.

Zaia, Joseph
Sethi, Manveen
Layne, Matthew
Raghunathan, Rekha

PMID: 35112150; PMCID: PMC8944212; DOI: 10.1007/s00216-022-03929-4;

Dance YW, Meshulam T, Seibel AJ, Obenreder MC, Layne MD, Nelson CM, Tien J. Adipose Stroma Accelerates the Invasion and Escape of Human Breast Cancer Cells from an Engineered Microtumor. Cell Mol Bioeng. 2022 Feb; 15(1):15-29.View Related Profiles.

Tien, Joe
Layne, Matthew
Meshulam, Tova

PMID: 35096184; PMCID: PMC8761189; DOI: 10.1007/s12195-021-00697-6;

Wang D, Rabhi N, Yet SF, Farmer SR, Layne MD. Aortic carboxypeptidase-like protein regulates vascular adventitial progenitor and fibroblast differentiation through myocardin related transcription factor A. Sci Rep. 2021 02 17; 11(1):3948.View Related Profiles.

Layne, Matthew
Rabhi, Nabil
Farmer, Stephen

PMID: 33597582; PMCID: PMC7889889; DOI: 10.1038/s41598-021-82941-7;

Hekman RM, Hume AJ, Goel RK, Abo KM, Huang J, Blum BC, Werder RB, Suder EL, Paul I, Phanse S, Youssef A, Alysandratos KD, Padhorny D, Ojha S, Mora-Martin A, Kretov D, Ash PEA, Verma M, Zhao J, Patten JJ, Villacorta-Martin C, Bolzan D, Perea-Resa C, Bullitt E, Hinds A, Tilston-Lunel A, Varelas X, Farhangmehr S, Braunschweig U, Kwan JH, McComb M, Basu A, Saeed M, Perissi V, Burks EJ, Layne MD, Connor JH, Davey R, Cheng JX, Wolozin BL, Blencowe BJ, Wuchty S, Lyons SM, Kozakov D, Cifuentes D, Blower M, Kotton DN, Wilson AA, Mühlberger E, Emili A. Actionable Cytopathogenic Host Responses of Human Alveolar Type 2 Cells to SARS-CoV-2. Mol Cell. 2021 Jan 07; 81(1):212.View Related Profiles. PMID: 33417854; PMCID: PMC7831449; DOI: 10.1016/j.molcel.2020.12.028;

Showing 10 of 85 results. Show More

Jiang WC, Chen CM, Hamdin CD, Orekhov AN, Sobenin IA, Layne MD, Yet SF. Therapeutic Potential of Heme Oxygenase-1 in Aneurysmal Diseases. Antioxidants (Basel). 2020 Nov 19; 9(11). PMID: 33228202; PMCID: PMC7699558; DOI: 10.3390/antiox9111150;

Hekman RM, Hume AJ, Goel RK, Abo KM, Huang J, Blum BC, Werder RB, Suder EL, Paul I, Phanse S, Youssef A, Alysandratos KD, Padhorny D, Ojha S, Mora-Martin A, Kretov D, Ash PEA, Verma M, Zhao J, Patten JJ, Villacorta-Martin C, Bolzan D, Perea-Resa C, Bullitt E, Hinds A, Tilston-Lunel A, Varelas X, Farhangmehr S, Braunschweig U, Kwan JH, McComb M, Basu A, Saeed M, Perissi V, Burks EJ, Layne MD, Connor JH, Davey R, Cheng JX, Wolozin BL, Blencowe BJ, Wuchty S, Lyons SM, Kozakov D, Cifuentes D, Blower M, Kotton DN, Wilson AA, Mühlberger E, Emili A. Actionable Cytopathogenic Host Responses of Human Alveolar Type 2 Cells to SARS-CoV-2. Mol Cell. 2020 12 17; 80(6):1104-1122.e9.View Related Profiles. PMID: 33259812; PMCID: PMC7674017; DOI: 10.1016/j.molcel.2020.11.028;

Zeng C, Motta-Ribeiro GC, Hinoshita T, Lessa MA, Winkler T, Grogg K, Kingston NM, Hutchinson JN, Sholl LM, Fang X, Varelas X, Layne MD, Baron RM, Vidal Melo MF. Lung Atelectasis Promotes Immune and Barrier Dysfunction as Revealed by Transcriptome Sequencing in Female Sheep. Anesthesiology. 2020 11 01; 133(5):1060-1076.View Related Profiles.

Layne, Matthew
Varelas, Xaralabos

PMID: 32796202; PMCID: PMC7572680; DOI: 10.1097/ALN.0000000000003491;

Vishwanath N, Monis WJ, Hoffmann GA, Ramachandran B, DiGiacomo V, Wong JY, Smith ML, Layne MD. Mechanisms of aortic carboxypeptidase-like protein secretion and identification of an intracellularly retained variant associated with Ehlers-Danlos syndrome. J Biol Chem. 2020 07 10; 295(28):9725-9735.View Related Profiles.

Wong, Joyce
Layne, Matthew
Smith, Michael

PMID: 32482891; PMCID: PMC7363150; DOI: 10.1074/jbc.RA120.013902;

Lee MJ, Pickering RT, Shibad V, Wu Y, Karastergiou K, Jager M, Layne MD, Fried SK. Impaired Glucocorticoid Suppression of TGFß Signaling in Human Omental Adipose Tissues Limits Adipogenesis and May Promote Fibrosis. Diabetes. 2019 03; 68(3):587-597.View Related Profiles.

Layne, Matthew
Jager, Mike
Pickering, Richard
Fried, Susan

PMID: 30530781; PMCID: PMC6385749; DOI: 10.2337/db18-0955;

Baron RM, Kwon MY, Castano AP, Ghanta S, Riascos-Bernal DF, Lopez-Guzman S, Macias AA, Ith B, Schissel SL, Lederer JA, Reeves R, Yet SF, Layne MD, Liu X, Perrella MA. Frontline Science: Targeted expression of a dominant-negative high mobility group A1 transgene improves outcome in sepsis. J Leukoc Biol. 2018 10; 104(4):677-689. PMID: 29975792; PMCID: PMC6431081; DOI: 10.1002/JLB.4HI0817-333RR;

Jager M, Lee MJ, Li C, Farmer SR, Fried SK, Layne MD. Aortic carboxypeptidase-like protein enhances adipose tissue stromal progenitor differentiation into myofibroblasts and is upregulated in fibrotic white adipose tissue. PLoS One. 2018; 13(5):e0197777.View Related Profiles.

Li, Chendi
Layne, Matthew
Jager, Mike
Farmer, Stephen
Fried, Susan

PMID: 29799877; PMCID: PMC5969754; DOI: 10.1371/journal.pone.0197777;

Blackburn PR, Xu Z, Tumelty KE, Zhao RW, Monis WJ, Harris KG, Gass JM, Cousin MA, Boczek NJ, Mitkov MV, Cappel MA, Francomano CA, Parisi JE, Klee EW, Faqeih E, Alkuraya FS, Layne MD, McDonnell NB, Atwal PS. Bi-allelic Alterations in AEBP1 Lead to Defective Collagen Assembly and Connective Tissue Structure Resulting in a Variant of Ehlers-Danlos Syndrome. Am J Hum Genet. 2018 04 05; 102(4):696-705.View Related Profiles.

Tumelty, Kathleen
Layne, Matthew

PMID: 29606302; PMCID: PMC5985336; DOI: 10.1016/j.ajhg.2018.02.018;

Shiwen X, Stratton R, Nikitorowicz-Buniak J, Ahmed-Abdi B, Ponticos M, Denton C, Abraham D, Takahashi A, Suki B, Layne MD, Lafyatis R, Smith BD. A Role of Myocardin Related Transcription Factor-A (MRTF-A) in Scleroderma Related Fibrosis. PLoS One. 2015; 10(5):e0126015.View Related Profiles.

Smith, Barbara
Suki, Bela
Layne, Matthew
Lafyatis, Robert

PMID: 25955164; PMCID: PMC4425676; DOI: 10.1371/journal.pone.0126015;

McDonald ME, Li C, Bian H, Smith BD, Layne MD, Farmer SR. Myocardin-related transcription factor A regulates conversion of progenitors to beige adipocytes. Cell. 2015 Jan 15; 160(1-2):105-18.View Related Profiles.

Smith, Barbara
Li, Chendi
Bian, Hejiao
Layne, Matthew
Farmer, Stephen

PMID: 25579684; PMCID: PMC4384505; DOI: 10.1016/j.cell.2014.12.005;

Xu YX, Ashline D, Liu L, Tassa C, Shaw SY, Ravid K, Layne MD, Reinhold V, Robbins PW. The glycosylation-dependent interaction of perlecan core protein with LDL: implications for atherosclerosis. J Lipid Res. 2015 Feb; 56(2):266-76.View Related Profiles.

Ravid, Katya
Layne, Matthew
Robbins, Phillips

PMID: 25528754; PMCID: PMC4306681; DOI: 10.1194/jlr.M053017;

Chen CH, Ho HH, Wu ML, Layne MD, Yet SF. Modulation of cysteine-rich protein 2 expression in vascular injury and atherosclerosis. Mol Biol Rep. 2014 Nov; 41(11):7033-41. PMID: 25034893; DOI: 10.1007/s11033-014-3591-x;

Wu ML, Chen CH, Lin YT, Jheng YJ, Ho YC, Yang LT, Chen L, Layne MD, Yet SF. Divergent signaling pathways cooperatively regulate TGFß induction of cysteine-rich protein 2 in vascular smooth muscle cells. Cell Commun Signal. 2014; 12:22. PMID: 24674138; PMCID: PMC3973006; DOI: 10.1186/1478-811X-12-22;

Tumelty KE, Smith BD, Nugent MA, Layne MD. Aortic carboxypeptidase-like protein (ACLP) enhances lung myofibroblast differentiation through transforming growth factor ß receptor-dependent and -independent pathways. J Biol Chem. 2014 Jan 31; 289(5):2526-36.View Related Profiles.

Smith, Barbara
Tumelty, Kathleen
Nugent, Matthew
Layne, Matthew

PMID: 24344132; PMCID: PMC3908388; DOI: 10.1074/jbc.M113.502617;

Chen CH, Ho YC, Ho HH, Chang IC, Kirsch KH, Chuang YJ, Layne MD, Yet SF. Cysteine-rich protein 2 alters p130Cas localization and inhibits vascular smooth muscle cell migration. Cardiovasc Res. 2013 Dec 1; 100(3):461-71.View Related Profiles.

Kirsch, Kathrin
Layne, Matthew

PMID: 23975851; PMCID: PMC3826702; DOI: 10.1093/cvr/cvt207;

Tumelty KE, Layne MD. Handbook of Proteolytic Enzymes (Rawlings, N. D. and Salvesen, G. S., ed.). Adipocyte Enhancer Binding Protein 1 and Aortic Carboxypeptidase-Like Protein. Academic Press. Oxford. 2013; 1348-1353.

Wang D, Prakash J, Nguyen P, Davis-Dusenbery BN, Hill NS, Layne MD, Hata A, Lagna G. Bone morphogenetic protein signaling in vascular disease: anti-inflammatory action through myocardin-related transcription factor A. J Biol Chem. 2012 Aug 10; 287(33):28067-77. PMID: 22718766; PMCID: PMC3431673; DOI: 10.1074/jbc.M112.379487;

Wu ML, Layne MD, Yet SF. Heme oxygenase-1 in environmental toxin-induced lung disease. Toxicol Mech Methods. 2012 Jun; 22(5):323-9. PMID: 22394342; DOI: 10.3109/15376516.2012.666685;

Zhou X, Baron RM, Hardin M, Cho MH, Zielinski J, Hawrylkiewicz I, Sliwinski P, Hersh CP, Mancini JD, Lu K, Thibault D, Donahue AL, Klanderman BJ, Rosner B, Raby BA, Lu Q, Geldart AM, Layne MD, Perrella MA, Weiss ST, Choi AM, Silverman EK. Identification of a chronic obstructive pulmonary disease genetic determinant that regulates HHIP. Hum Mol Genet. 2012 Mar 15; 21(6):1325-35. PMID: 22140090; PMCID: PMC3284120; DOI: 10.1093/hmg/ddr569;

Luchsinger LL, Patenaude CA, Smith BD, Layne MD. Myocardin-related transcription factor-A complexes activate type I collagen expression in lung fibroblasts. J Biol Chem. 2011 Dec 23; 286(51):44116-25.View Related Profiles.

Smith, Barbara
Layne, Matthew

PMID: 22049076; PMCID: PMC3243519; DOI: 10.1074/jbc.M111.276931;

Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A. down-regulation of Kruppel-like factor-4 (KLF4) by microRNA-143/145 is critical for modulation of vascular smooth muscle cell phenotype by transforming growth factor-beta and bone morphogenetic protein 4. J Biol Chem. 2011 Aug 12; 286(32):28097-110. PMID: 21673106; PMCID: PMC3151055; DOI: 10.1074/jbc.M111.236950;

Danzer E, Layne MD, Auber F, Shegu S, Kreiger P, Radu A, Volpe M, Adzick NS, Flake AW. Gastroschisis in mice lacking aortic carboxypeptidase-like protein is associated with a defect in neuromuscular development of the eviscerated intestine. Pediatr Res. 2010 Jul; 68(1):23-8. PMID: 20386491; DOI: 10.1203/00006450-201011001-00040;

Baron RM, Lopez-Guzman S, Riascos DF, Macias AA, Layne MD, Cheng G, Harris C, Chung SW, Reeves R, von Andrian UH, Perrella MA. Distamycin A inhibits HMGA1-binding to the P-selectin promoter and attenuates lung and liver inflammation during murine endotoxemia. PLoS One. 2010; 5(5):e10656. PMID: 20498830; PMCID: PMC2871042; DOI: 10.1371/journal.pone.0010656;

Chen CH, Wu ML, Lee YC, Layne MD, Yet SF. Intronic CArG box regulates cysteine-rich protein 2 expression in the adult but not in developing vasculature. Arterioscler Thromb Vasc Biol. 2010 Apr; 30(4):835-42. PMID: 20075421; PMCID: PMC2841712; DOI: 10.1161/ATVBAHA.109.197251;

Grant MA, Baron RM, Macias AA, Layne MD, Perrella MA, Rigby AC. Netropsin improves survival from endotoxaemia by disrupting HMGA1 binding to the NOS2 promoter. Biochem J. 2009 Feb 15; 418(1):103-12. PMID: 18937643; PMCID: PMC2821790; DOI: 10.1042/BJ20081427;

Schissel SL, Dunsmore SE, Liu X, Shine RW, Perrella MA, Layne MD. Aortic carboxypeptidase-like protein is expressed in fibrotic human lung and its absence protects against bleomycin-induced lung fibrosis. Am J Pathol. 2009 Mar; 174(3):818-28. PMID: 19179605; PMCID: PMC2665743; DOI: 10.2353/ajpath.2009.080856;

Liu X, Ramjiganesh T, Chen YH, Chung SW, Hall SR, Schissel SL, Padera RF, Liao R, Ackerman KG, Kajstura J, Leri A, Anversa P, Yet SF, Layne MD, Perrella MA. Disruption of striated preferentially expressed gene locus leads to dilated cardiomyopathy in mice. Circulation. 2009 Jan 20; 119(2):261-8. PMID: 19118250; PMCID: PMC2630246; DOI: 10.1161/CIRCULATIONAHA.108.799536;

Lin DW, Chang IC, Tseng A, Wu ML, Chen CH, Patenaude CA, Layne MD, Yet SF. Transforming growth factor beta up-regulates cysteine-rich protein 2 in vascular smooth muscle cells via activating transcription factor 2. J Biol Chem. 2008 May 30; 283(22):15003-14. PMID: 18387947; PMCID: PMC2397482; DOI: 10.1074/jbc.M801621200;

Takamiya R, Baron RM, Yet SF, Layne MD, Perrella MA. High mobility group A1 protein mediates human nitric oxide synthase 2 gene expression. FEBS Lett. 2008 Mar 5; 582(5):810-4. PMID: 18279675; PMCID: PMC2271050; DOI: 10.1016/j.febslet.2008.02.008;

Zurakowski H, Gagnon A, Landry A, Layne MD, Sorisky A. Discoidin domain receptor 2 impairs insulin-stimulated insulin receptor substrate-1 tyrosine phosphorylation and glucose uptake in 3T3-L1 adipocytes. Horm Metab Res. 2007 Aug; 39(8):575-81. PMID: 17712722

Schissel SL, Layne MD. Telomerase, myofibroblasts, and pulmonary fibrosis. Am J Respir Cell Mol Biol. 2006 May; 34(5):520-2. PMID: 16618786

Chung SW, Chen YH, Yet SF, Layne MD, Perrella MA. Endotoxin-induced down-regulation of Elk-3 facilitates heme oxygenase-1 induction in macrophages. J Immunol. 2006 Feb 15; 176(4):2414-20. PMID: 16456000

Wei J, Gorman TE, Liu X, Ith B, Tseng A, Chen Z, Simon DI, Layne MD, Yet SF. Increased neointima formation in cysteine-rich protein 2-deficient mice in response to vascular injury. Circ Res. 2005 Dec 9; 97(12):1323-31. PMID: 16269651

Gagnon A, Landry A, Proulx J, Layne MD, Sorisky A. Aortic carboxypeptidase-like protein is regulated by transforming growth factor beta in 3T3-L1 preadipocytes. Exp Cell Res. 2005 Aug 15; 308(2):265-72. PMID: 15927179

Liu X, Wei J, Peng DH, Layne MD, Yet SF. Absence of heme oxygenase-1 exacerbates myocardial ischemia/reperfusion injury in diabetic mice. Diabetes. 2005 Mar; 54(3):778-84. PMID: 15734856

Ith B, Wei J, Yet SF, Perrella MA, Layne MD. Aortic carboxypeptidase-like protein is expressed in collagen-rich tissues during mouse embryonic development. Gene Expr Patterns. 2005 Apr; 5(4):533-7. PMID: 15749083

Baron RM, Carvajal IM, Liu X, Okabe RO, Fredenburgh LE, Macias AA, Chen YH, Ejima K, Layne MD, Perrella MA. Reduction of nitric oxide synthase 2 expression by distamycin A improves survival from endotoxemia. J Immunol. 2004 Sep 15; 173(6):4147-53. PMID: 15356165

Agrawal RS, Muangman S, Layne MD, Melo L, Perrella MA, Lee RT, Zhang L, Lopez-Ilasaca M, Dzau VJ. Pre-emptive gene therapy using recombinant adeno-associated virus delivery of extracellular superoxide dismutase protects heart against ischemic reperfusion injury, improves ventricular function and prolongs survival. Gene Ther. 2004 Jun; 11(12):962-9. PMID: 15029230

Chen YH, Layne MD, Chung SW, Ejima K, Baron RM, Yet SF, Perrella MA. Elk-3 is a transcriptional repressor of nitric-oxide synthase 2. J Biol Chem. 2003 Oct 10; 278(41):39572-7. PMID: 12896968

Yet SF, Layne MD, Liu X, Chen YH, Ith B, Sibinga NE, Perrella MA. Absence of heme oxygenase-1 exacerbates atherosclerotic lesion formation and vascular remodeling. FASEB J. 2003 Sep; 17(12):1759-61. PMID: 12958201

Chang YF, Wei J, Liu X, Chen YH, Layne MD, Yet SF. Identification of a CArG-independent region of the cysteine-rich protein 2 promoter that directs expression in the developing vasculature. Am J Physiol Heart Circ Physiol. 2003 Oct; 285(4):H1675-83. PMID: 12791591

Ejima K, Layne MD, Carvajal IM, Kritek PA, Baron RM, Chen YH, Vom Saal J, Levy BD, Yet SF, Perrella MA. Cyclooxygenase-2-deficient mice are resistant to endotoxin-induced inflammation and death. FASEB J. 2003 Jul; 17(10):1325-7. PMID: 12738799

Watanabe M, Layne MD, Hsieh CM, Maemura K, Gray S, Lee ME, Jain MK. Regulation of smooth muscle cell differentiation by AT-rich interaction domain transcription factors Mrf2alpha and Mrf2beta. Circ Res. 2002 Sep 6; 91(5):382-9. PMID: 12215486

Ejima K, Layne MD, Carvajal IM, Nanri H, Ith B, Yet SF, Perrella MA. Modulation of the thioredoxin system during inflammatory responses and its effect on heme oxygenase-1 expression. Antioxid Redox Signal. 2002 Aug; 4(4):569-75. PMID: 12230868

Gagnon A, Abaiian KJ, Crapper T, Layne MD, Sorisky A. Down-regulation of aortic carboxypeptidase-like protein during the early phase of 3T3-L1 adipogenesis. Endocrinology. 2002 Jul; 143(7):2478-85. PMID: 12072377

Storm van's Gravesande K, Layne MD, Ye Q, Le L, Baron RM, Perrella MA, Santambrogio L, Silverman ES, Riese RJ. IFN regulatory factor-1 regulates IFN-gamma-dependent cathepsin S expression. J Immunol. 2002 May 1; 168(9):4488-94. PMID: 11970993

Layne MD, Yet SF, Maemura K, Hsieh CM, Liu X, Ith B, Lee ME, Perrella MA. Characterization of the mouse aortic carboxypeptidase-like protein promoter reveals activity in differentiated and dedifferentiated vascular smooth muscle cells. Circ Res. 2002 Apr 5; 90(6):728-36. PMID: 11934842

Melo LG, Agrawal R, Zhang L, Rezvani M, Mangi AA, Ehsan A, Griese DP, Dell'Acqua G, Mann MJ, Oyama J, Yet SF, Layne MD, Perrella MA, Dzau VJ. Gene therapy strategy for long-term myocardial protection using adeno-associated virus-mediated delivery of heme oxygenase gene. Circulation. 2002 Feb 5; 105(5):602-7. PMID: 11827926

Yet SF, Melo LG, Layne MD, Perrella MA. Heme oxygenase 1 in regulation of inflammation and oxidative damage. Methods Enzymol. 2002; 353:163-76. PMID: 12078492

Maemura K, Layne MD, Watanabe M, Perrell MA, Nagai R, Lee ME. Molecular mechanisms of morning onset of myocardial infarction. Ann N Y Acad Sci. 2001 Dec; 947:398-402. PMID: 11795300

Chen YH, Layne MD, Watanabe M, Yet SF, Perrella MA. Upstream stimulatory factors regulate aortic preferentially expressed gene-1 expression in vascular smooth muscle cells. J Biol Chem. 2001 Dec 14; 276(50):47658-63. PMID: 11606591

Layne MD, Patel A, Chen YH, Rebel VI, Carvajal IM, Pellacani A, Ith B, Zhao D, Schreiber BM, Yet SF, Lee ME, Storch J, Perrella MA. Role of macrophage-expressed adipocyte fatty acid binding protein in the development of accelerated atherosclerosis in hypercholesterolemic mice. FASEB J. 2001 Dec; 15(14):2733-5.View Related Profiles.

Schreiber, Barbara
Layne, Matthew

PMID: 11606480

Perrella MA, Pellacani A, Layne MD, Patel A, Zhao D, Schreiber BM, Storch J, Feinberg MW, Hsieh CM, Haber E, Lee ME. Absence of adipocyte fatty acid binding protein prevents the development of accelerated atherosclerosis in hypercholesterolemic mice. FASEB J. 2001 Aug; 15(10):1774-6.View Related Profiles.

Schreiber, Barbara
Layne, Matthew

PMID: 11481226

Layne MD, Yet SF, Maemura K, Hsieh CM, Bernfield M, Perrella MA, Lee ME. Impaired abdominal wall development and deficient wound healing in mice lacking aortic carboxypeptidase-like protein. Mol Cell Biol. 2001 Aug; 21(15):5256-61. PMID: 11438679; PMCID: PMC87249

Yet SF, Tian R, Layne MD, Wang ZY, Maemura K, Solovyeva M, Ith B, Melo LG, Zhang L, Ingwall JS, Dzau VJ, Lee ME, Perrella MA. Cardiac-specific expression of heme oxygenase-1 protects against ischemia and reperfusion injury in transgenic mice. Circ Res. 2001 Jul 20; 89(2):168-73. PMID: 11463724

Wiesel P, Patel AP, Carvajal IM, Wang ZY, Pellacani A, Maemura K, DiFonzo N, Rennke HG, Layne MD, Yet SF, Lee ME, Perrella MA. Exacerbation of chronic renovascular hypertension and acute renal failure in heme oxygenase-1-deficient mice. Circ Res. 2001 May 25; 88(10):1088-94. PMID: 11375280

Fukumoto S, Hsieh CM, Maemura K, Layne MD, Yet SF, Lee KH, Matsui T, Rosenzweig A, Taylor WG, Rubin JS, Perrella MA, Lee ME. Akt participation in the Wnt signaling pathway through Dishevelled. J Biol Chem. 2001 May 18; 276(20):17479-83. PMID: 11278246

Sun J, Kamei CN, Layne MD, Jain MK, Liao JK, Lee ME, Chin MT. Regulation of myogenic terminal differentiation by the hairy-related transcription factor CHF2. J Biol Chem. 2001 May 25; 276(21):18591-6. PMID: 11279181

Hsieh CM, Fukumoto S, Layne MD, Maemura K, Charles H, Patel A, Perrella MA, Lee ME. Striated muscle preferentially expressed genes alpha and beta are two serine/threonine protein kinases derived from the same gene as the aortic preferentially expressed gene-1. J Biol Chem. 2000 Nov 24; 275(47):36966-73. PMID: 10973969

Maemura K, de la Monte SM, Chin MT, Layne MD, Hsieh CM, Yet SF, Perrella MA, Lee ME. CLIF, a novel cycle-like factor, regulates the circadian oscillation of plasminogen activator inhibitor-1 gene expression. J Biol Chem. 2000 Nov 24; 275(47):36847-51. PMID: 11018023

Wiesel P, Foster LC, Pellacani A, Layne MD, Hsieh CM, Huggins GS, Strauss P, Yet SF, Perrella MA. Thioredoxin facilitates the induction of heme oxygenase-1 in response to inflammatory mediators. J Biol Chem. 2000 Aug 11; 275(32):24840-6. PMID: 10823822

Chin MT, Maemura K, Fukumoto S, Jain MK, Layne MD, Watanabe M, Hsieh CM, Lee ME. Cardiovascular basic helix loop helix factor 1, a novel transcriptional repressor expressed preferentially in the developing and adult cardiovascular system. J Biol Chem. 2000 Mar 3; 275(9):6381-7. PMID: 10692439

Maemura K, Hsieh CM, Jain MK, Fukumoto S, Layne MD, Liu Y, Kourembanas S, Yet SF, Perrella MA, Lee ME. Generation of a dominant-negative mutant of endothelial PAS domain protein 1 by deletion of a potent C-terminal transactivation domain. J Biol Chem. 1999 Oct 29; 274(44):31565-70. PMID: 10531360

Layne MD, Farmer SR. Tumor necrosis factor-alpha and basic fibroblast growth factor differentially inhibit the insulin-like growth factor-I induced expression of myogenin in C2C12 myoblasts. Exp Cell Res. 1999 May 25; 249(1):177-87.View Related Profiles.

Layne, Matthew
Farmer, Stephen

PMID: 10328964

Hsieh CM, Yet SF, Layne MD, Watanabe M, Hong AM, Perrella MA, Lee ME. Genomic cloning and promoter analysis of aortic preferentially expressed gene-1. Identification of a vascular smooth muscle-specific promoter mediated by an E box motif. J Biol Chem. 1999 May 14; 274(20):14344-51. PMID: 10318857

Yet SF, Perrella MA, Layne MD, Hsieh CM, Maemura K, Kobzik L, Wiesel P, Christou H, Kourembanas S, Lee ME. Hypoxia induces severe right ventricular dilatation and infarction in heme oxygenase-1 null mice. J Clin Invest. 1999 Apr; 103(8):R23-9. PMID: 10207174; PMCID: PMC408281

Jain MK, Kashiki S, Hsieh CM, Layne MD, Yet SF, Sibinga NE, Chin MT, Feinberg MW, Woo I, Maas RL, Haber E, Lee ME. Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular system. Circ Res. 1998 Nov 16; 83(10):980-5. PMID: 9815145

Layne MD, Endege WO, Jain MK, Yet SF, Hsieh CM, Chin MT, Perrella MA, Blanar MA, Haber E, Lee ME. Aortic carboxypeptidase-like protein, a novel protein with discoidin and carboxypeptidase-like domains, is up-regulated during vascular smooth muscle cell differentiation. J Biol Chem. 1998 Jun 19; 273(25):15654-60. PMID: 9624159

Yet SF, Folta SC, Jain MK, Hsieh CM, Maemura K, Layne MD, Zhang D, Marria PB, Yoshizumi M, Chin MT, Perrella MA, Lee ME. Molecular cloning, characterization, and promoter analysis of the mouse Crp2/SmLim gene. Preferential expression of its promoter in the vascular smooth muscle cells of transgenic mice. J Biol Chem. 1998 Apr 24; 273(17):10530-7. PMID: 9553112

Jain MK, Layne MD, Watanabe M, Chin MT, Feinberg MW, Sibinga NE, Hsieh CM, Yet SF, Stemple DL, Lee ME. In vitro system for differentiating pluripotent neural crest cells into smooth muscle cells. J Biol Chem. 1998 Mar 13; 273(11):5993-6. PMID: 9497310

Yet SF, McA'Nulty MM, Folta SC, Yen HW, Yoshizumi M, Hsieh CM, Layne MD, Chin MT, Wang H, Perrella MA, Jain MK, Lee ME. Human EZF, a Krüppel-like zinc finger protein, is expressed in vascular endothelial cells and contains transcriptional activation and repression domains. J Biol Chem. 1998 Jan 9; 273(2):1026-31. PMID: 9422764

Layne MD. Regulation of myogenin expression and function during myoblast proliferation and differentiation [PhD dissertation]. Boston, MA. 1997.

Zoeller RA, Layne MD, Modest EJ. Animal cell mutants unable to take up biologically active glycerophospholipids. J Lipid Res. 1995 Sep; 36(9):1866-75.View Related Profiles.

Layne, Matthew
Zoeller, Raphael

PMID: 8558075

Schreiber BM, Layne MD, Modest EJ. Superoxide production by macrophages stimulated in vivo with synthetic ether lipids. Lipids. 1994 Apr; 29(4):237-42.View Related Profiles.

Schreiber, Barbara
Layne, Matthew

PMID: 8177017

Morris-Natschke SL, Gumus F, Marasco CJ, Meyer KL, Marx M, Piantadosi C, Layne MD, Modest EJ. Synthesis of phosphocholine and quaternary amine ether lipids and evaluation of in vitro antineoplastic activity. J Med Chem. 1993 Jul 9; 36(14):2018-25. PMID: 8336340

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 85 publications over 28 distinct years, with a maximum of 9 publications in 2001

Bar chart showing 85 publications over 28 distinct years, with a maximum of 9 publications in 2001

Year	Publications
1993	1
1994	1
1995	1
1997	1
1998	5
1999	4
2000	4
2001	9
2002	7
2003	4
2004	3
2005	3
2006	2
2007	1
2008	3
2009	2
2010	3
2011	3
2012	2
2013	3
2014	3
2015	2
2018	4
2020	4
2021	3
2022	4
2023	2
2024	1

2022 Graduate Medical Sciences. Boston University School of Medicine: Educator of the Year Award, PhD Program

2018 Graduate Medical Sciences, Boston University School of Medicine: Faculty Recognition Award

2011 Fellow of the American Heart Association

In addition to these self-described keywords below, a list of MeSH based concepts is available here.

Extracellular Matrix Proteins, Ehlers-Danlos syndrome, fibrosis, mechanostransduction, collagen
adipose tissue

My research interests include mechanisms of smooth muscle changes in vascular disease, fibrosis, and now cancer. In a research or laboratory setting I have mentored numerous scientists at the undergraduate, graduate, and postdoctoral levels and have participated on more than 25 PhD dissertation committees.

In 2021, I was appointed as the Assistant Dean for Research. In this role, I mentor BUSM medical students in their research.

Available to Mentor as: (Review Mentor Role Definitions):