Matthew D. Layne, PhD
Associate Professor
Boston University School of Medicine
Dept of Biochemistry

PhD, Boston University School of Medicine

The primary goal of our laboratory is to identify novel pathways that control extracellular matrix(ECM) synthesis and assembly as they relate to fibroproliferative and connective tissue diseases. Our long term goal is to use this knowledge to develop therapeutic strategies for these conditions. Fibroproliferative responses are similar to wound healing processes involving accumulation of contractile myofibroblasts and ECM secretion and assembly. Because organ fibrosis, cardiovascular, metabolic/obesity, and cancer pathologies are now recognized to be impacted by fibroblast-myofibroblast differentiation and ECM remodeling our research is examining novel pathways and control mechanisms in these diseases. Central to our studies is determining the function of Aortic Carboxypeptidase-Like Protein (ACLP), a secreted, collagen-binding protein that enhances fibrosis and myofibroblast differentiation through mechanisms that involve stimulating the transforming growth factor ß (TGFß) receptor signaling complex and controlling mechanical signaling and ECM remodeling. Recent work is uncovering the role of ACLP (and AEBP1 genetic mutations in the connective tissue disease Ehlers Danlos Syndrome. There are several active projects in the lab including:

• Defining the the role of ACLP in mechanotransduction pathways that control progenitor differentiation.
• Investigating the mechanisms of how ACLP/AEBP1 mutations cause Ehlers Danlos Syndrome (EDS)
• Developing strategies to organ fibrosis through targeting ACLP
• Studying the stromal reaction in breast cancer.
• Uncovering new mechanisms that control adipose tissue fibrosis.

Graduate Faculty (Primary Mentor of Grad Students)
Boston University School of Medicine, Division of Graduate Medical Sciences

Member of the Cell and Molecular Biology Program
Boston University School of Medicine

Member of the Molecular Medicine Program
Boston University School of Medicine

2011 Fellow of the American Heart Association

Development of Tumor-Directed Therapies
05/01/2015 - 04/30/2018 (PI)
Department of Defense/Army Medical Research Acquisition Activity

Role of ACLP in Adipocyte Determination and Fibrosis
01/21/2016 - 03/31/2017 (PI)
Boston Medical Center Corporation NIH NIDDK

ACLP-mediated Vascular Advential Fibroblast Differentiation and Remodeling
01/01/2014 - 12/31/2016 (PI)
American Heart Association

Inhibitors of Aortic Carboxypeptides-like Proten to Treat Fibroproliferative Diseases
01/01/2015 - 07/02/2016 (PI)
Pfizer, Inc.

Role of ACLP in adipocyte determination and fibrosis
04/01/2012 - 03/31/2014 (PI)
Boston Medical Center Corporation NIH NIDDK

Yr Title Project-Sub Proj Pubs
2011 Role of ACLP in Vascular Smooth Muscle Biology 5R01HL078869-06 10
2010 Role of ACLP in Vascular Smooth Muscle Biology 5R01HL078869-05 10
2009 Role of ACLP in Vascular Smooth Muscle Biology 3R01HL078869-04S1 10
2009 Role of ACLP in Vascular Smooth Muscle Biology 5R01HL078869-04 10
2008 Role of ACLP in Vascular Smooth Muscle Biology 5R01HL078869-03 10
2007 Role of ACLP in Vascular Smooth Muscle Biology 1R01HL078869-01A2 10
2007 Role of ACLP in Vascular Smooth Muscle Biology 7R01HL078869-02 10
2004 Role of ACLP in dermal wound healing 5K01AR047861-04 4
2003 Role of ACLP in dermal wound healing 5K01AR047861-03 4
2002 Role of ACLP in dermal wound healing 5K01AR047861-02 4
Showing 10 of 13 results. Show All Results
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

  1. Blackburn PR, Xu Z, Tumelty KE, Zhao RW, Monis WJ, Harris KG, Gass JM, Cousin MA, Boczek NJ, Mitkov MV, Cappel MA, Francomano CA, Parisi JE, Klee EW, Faqeih E, Alkuraya FS, Layne MD, McDonnell NB, Atwal PS. Biallelic Alterations in AEBP1 Lead to Defective Collagen Assembly and Connective Tissue Structure Resulting in a Variant of Ehlers-Danlos Syndrome. Am J Human Genetics, in press. 2018.
  2. Shiwen X, Stratton R, Nikitorowicz-Buniak J, Ahmed-Abdi B, Ponticos M, Denton C, Abraham D, Takahashi A, Suki B, Layne MD, Lafyatis R, Smith BD. A Role of Myocardin Related Transcription Factor-A (MRTF-A) in Scleroderma Related Fibrosis. PLoS One. 2015; 10(5):e0126015.View Related Profiles. PMID: 25955164; PMCID: PMC4425676; DOI: 10.1371/journal.pone.0126015;.
  3. McDonald ME, Li C, Bian H, Smith BD, Layne MD, Farmer SR. Myocardin-related transcription factor A regulates conversion of progenitors to beige adipocytes. Cell. 2015 Jan 15; 160(1-2):105-18.View Related Profiles. PMID: 25579684; PMCID: PMC4384505; DOI: 10.1016/j.cell.2014.12.005;.
  4. Xu YX, Ashline D, Liu L, Tassa C, Shaw SY, Ravid K, Layne MD, Reinhold V, Robbins PW. The glycosylation-dependent interaction of perlecan core protein with LDL: implications for atherosclerosis. J Lipid Res. 2015 Feb; 56(2):266-76.View Related Profiles. PMID: 25528754; PMCID: PMC4306681; DOI: 10.1194/jlr.M053017;.
  5. Chen CH, Ho HH, Wu ML, Layne MD, Yet SF. Modulation of cysteine-rich protein 2 expression in vascular injury and atherosclerosis. Mol Biol Rep. 2014 Nov; 41(11):7033-41. PMID: 25034893; DOI: 10.1007/s11033-014-3591-x;.
  6. Wu ML, Chen CH, Lin YT, Jheng YJ, Ho YC, Yang LT, Chen L, Layne MD, Yet SF. Divergent signaling pathways cooperatively regulate TGFß induction of cysteine-rich protein 2 in vascular smooth muscle cells. Cell Commun Signal. 2014; 12:22. PMID: 24674138; PMCID: PMC3973006; DOI: 10.1186/1478-811X-12-22;.
  7. Tumelty KE, Smith BD, Nugent MA, Layne MD. Aortic carboxypeptidase-like protein (ACLP) enhances lung myofibroblast differentiation through transforming growth factor ß receptor-dependent and -independent pathways. J Biol Chem. 2014 Jan 31; 289(5):2526-36.View Related Profiles. PMID: 24344132; PMCID: PMC3908388; DOI: 10.1074/jbc.M113.502617;.
  8. Chen CH, Ho YC, Ho HH, Chang IC, Kirsch KH, Chuang YJ, Layne MD, Yet SF. Cysteine-rich protein 2 alters p130Cas localization and inhibits vascular smooth muscle cell migration. Cardiovasc Res. 2013 Dec 1; 100(3):461-71.View Related Profiles. PMID: 23975851; PMCID: PMC3826702; DOI: 10.1093/cvr/cvt207;.
  9. Tumelty KE, Layne MD. Handbook of Proteolytic Enzymes (Rawlings, N. D. and Salvesen, G. S., ed.). Adipocyte Enhancer Binding Protein 1 and Aortic Carboxypeptidase-Like Protein. Academic Press. Oxford. 2013; 1348-1353.
  10. Wang D, Prakash J, Nguyen P, Davis-Dusenbery BN, Hill NS, Layne MD, Hata A, Lagna G. Bone morphogenetic protein signaling in vascular disease: anti-inflammatory action through myocardin-related transcription factor A. J Biol Chem. 2012 Aug 10; 287(33):28067-77. PMID: 22718766; PMCID: PMC3431673; DOI: 10.1074/jbc.M112.379487;.
Showing 10 of 68 results. Show More

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 68 publications over 23 distinct years, with a maximum of 9 publications in 2001


My research interests include mechanisms of smooth muscle changes in vascular disease, fibrosis, and now cancer. In a research or laboratory setting I have mentored more than 20 scientists at the undergraduate, graduate, and postdoctoral levels. I have also participated on more 25 PhD dissertation committees. Currently I serve as an academic advisor in the Master's in Medical Sciences (MAMS) program.

Available to Mentor as: (Review Mentor Role Definitions):
  • Advisor
  • Education Mentor
  • Project Mentor
  • Research / Scholarly Mentor
Contact for Mentoring:
  • Email (see 'Contact Info')

72 E. Concord St Silvio Conte (K)
Boston MA 02118
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