John Ngo, PhD
Associate Professor
Boston University College of Engineering
Biomedical Engineering

PhD, California Institute of Technology
BA, University of California, Santa Barbara

I was the first my family to finish high school and the first to earn a Ph.D. My training in research began at UC Santa Barbara, where I characterized enzymes involved in biosilica formation in the “orange puffball sponge” Tethya aurantium. As a graduate student at Caltech, I developed cell-targeted methods to track de novo protein synthesis in neurons, tissues, and in vivo. After my Ph.D., I trained with Roger Tsien on probe development for imaging cells via correlative light and electron microscopy (CLEM). At Boston University, my lab investigates mechanisms of signal transduction at cell-cell interfaces. Our current efforts are focused on Notch signal transduction, in which we seek to understand how mechanical tension is delivered to and sensed by Notch receptors for the regulation their protease sensitivity and signaling activities. Students from the lab have received several awards and honors, including the BME Thesis of the Year Award and the College of Engineering's Earle and Mildred Bailey Memorial Award, among others.

Integrative Approaches for Probing Cell Mechanotransduction in Health and Disease
08/23/2018 - 07/31/2024 (PI)
NIH/National Institute of General Medical Sciences

New Probe and Methods for Correlated LM & EM
08/17/2017 - 08/23/2018 (Subcontract PI)
University of California, San Diego NIH NIGMS


Yr Title Project-Sub Proj Pubs

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

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  1. Tran JC, Kuffner CJ, Marzilli AM, Miller RE, Silfen ZE, McMahan JB, Sloas DC, Chen CS, Ngo JT. Fluorescein-Based SynNotch Adaptors for Regulating Gene Expression Responses to Diverse Extracellular Cues. bioRxiv. 2024 Jun 12. PMID: 38915575; PMCID: PMC11195177; DOI: 10.1101/2024.06.12.598538;
  2. Adams SR, Mackey MR, Ramachandra R, Deerinck TJ, Castillon GA, Phan S, Hu J, Boassa D, Ngo JT, Ellisman MH. Fe-TAMLs as a new class of small molecule peroxidase probes for correlated light and electron microscopy. bioRxiv. 2023 Oct 04. PMID: 37662194; PMCID: PMC10473768; DOI: 10.1101/2023.08.25.554352;
  3. Tague EP, McMahan JB, Tague N, Dunlop MJ, Ngo JT. Controlled Protein Activities with Viral Proteases, Antiviral Peptides, and Antiviral Drugs. ACS Chem Biol. 2023 May 19; 18(5):1228-1236.View Related Profiles. PMID: 37140437; PMCID: PMC10501127; DOI: 10.1021/acschembio.3c00138;
  4. Tague EP, McMahan JB, Tague N, Dunlop MJ, Ngo JT. Controlled protein activities with viral proteases, antiviral peptides, and antiviral drugs. bioRxiv. 2023 Feb 27.View Related Profiles. PMID: 36909459; PMCID: PMC10002686; DOI: 10.1101/2023.02.27.530290;
  5. Sloas DC, Tran JC, Marzilli AM, Ngo JT. Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection. Nat Biotechnol. 2023 Sep; 41(9):1287-1295. PMID: 36646932; PMCID: PMC10499187; DOI: 10.1038/s41587-022-01638-y;
  6. Li HS, Wong NM, Tague E, Ngo JT, Khalil AS, Wong WW. High-performance multiplex drug-gated CAR circuits. Cancer Cell. 2022 Nov 14; 40(11):1294-1305.e4.View Related Profiles. PMID: 36084652; PMCID: PMC9669166; DOI: 10.1016/j.ccell.2022.08.008;
  7. Marzilli AM, McMahan JB, Ngo JT. Precision control of intrabodies in live cells. Nat Methods. 2020 03; 17(3):259-260. PMID: 32076290; DOI: 10.1038/s41592-020-0767-2;
  8. Li H-S, Wong NM, Tague E, Ngo JT, Khalil AS, Wong WW. Engineering clinically-approved drug gated CAR circuits. Biorxiv. 2020.
  9. Boassa D, Lemieux SP, Lev-Ram V, Hu J, Xiong Q, Phan S, Mackey M, Ramachandra R, Peace RE, Adams SR, Ellisman MH, Ngo JT. Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy. Cell Chem Biol. 2019 10 17; 26(10):1407-1416.e5. PMID: 31378710; PMCID: PMC6800643; DOI: 10.1016/j.chembiol.2019.07.007;
  10. Boassa D, Ngo JT, Mackey MR, Ramachandra R, Deerinck TJ, Ellisman MH, Adams SR. New Molecular Tools for Protein Detection and Dynamics in Electron Microscopy. Microscopy and Microanalysis. 2019; 25(S2).
Showing 10 of 24 results. Show More

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 24 publications over 14 distinct years, with a maximum of 4 publications in 2023

Contact for Mentoring:

44 Cummington St, Boston MA 02215
Boston MA 02215
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