Jacqueline Sharon | Profiles RNS

Jacqueline Sharon, PhD

Emeritus Professor

Boston University Chobanian & Avedisian School of Medicine

Pathology & Laboratory Medicine

Other Pathology

PhD, Columbia University
MPhil, Columbia University
MA, Queens College, City University of New York
BA, Queens College, City University of New York

Websites

	Linkedin
	ResearchGate

ORCID : 0000-0002-8950-1615

Jacqueline Sharon, PhD is a current Professor of Pathology and Laboratory Medicine at the Chobanian and Avedisian School of Medicine. After earning her PhD at Columbia University, she completed a Postdoc at Harvard University. She currently receives funding from the National Institutes of Health for her research on B Cell Epitope Discovery and Mechanisms of Antibody Protection. Dr. Sharon's laboratory is interested in the development of vaccines and immunotherapeutics against bacterial pathogens. Current research focuses on the discovery and validation of antigens and epitopes that induce protective antibodies to Francisella tularensis, a Gram negative facultative intracellular bacterium that causes tularemia.

Research Associate Professor
Boston University Chobanian & Avedisian School of Medicine
Biochemistry & Cell Biology

Member
Boston University
BU-BMC Cancer Center

Member
Boston University
Evans Center for Interdisciplinary Biomedical Research

Graduate Faculty (Primary Mentor of Grad Students)
Boston University Chobanian & Avedisian School of Medicine, Graduate Medical Sciences

BU Research
NIH RePORTER Research

Protective and Pathogenic Bcell Epitopes in Tularemia
09/30/2009 - 09/29/2014 (Subcontract PI)
HHS/PHS (Public Health Contracts) NIH NIAID

Polyclonal Antibody Libraries for Tularemia
09/01/2003 - 02/28/2009 (PI)
Boston Medical Center NIH NIAID
R21 AI83832-01

Polyclonal Antibody Libraries (Continuation of Structural Requirements of Antibody Combining Sites).
10/01/2001 - 08/31/2004 (PI)
Symphogen A/S

Recombinant Polyclonal Antibody Libraries for Breast Cancer Therapy
09/01/1998 - 08/31/2001 (PI)
Department of Defense/Army

Structural Requirements of Antibody Combining Sites
07/01/1996 - 06/30/2001 (PI)
NIH/National Institute of Allergy & Infectious Diseases
5 R01 AI23909 14

Title

Yr	Title	Project-Sub Proj	Pubs
2011	STRUCTURAL ANALYSIS OF O-ANTIGEN FROM F TULARENSIS	5P41RR010888-15-5153	259
2010	STRUCTURAL ANALYSIS OF O-ANTIGEN FROM F TULARENSIS	5P41RR010888-14-6861	259
2010	Protective and pathogenic B cell epitopes in Francisella tularensis	272200900054C-1-0-1
2009	Protective and pathogenic B cell epitopes in Francisella tularensis	272200900054C-0-0-1
2000	STRUCTURAL REQUIREMENTS OF ANTIBODY COMBINING SITES	5R01AI023909-14	25
1999	POLYCLONAL ANTIBODY LIBRARIES FOR C PARVUM THERAPY	5U01AI040344-04	5
1999	STRUCTURAL REQUIREMENTS OF ANTIBODY COMBINING SITES	5R01AI023909-13	25
1998	POLYCLONAL ANTIBODY LIBRARIES FOR C PARVUM THERAPY	5U01AI040344-03	5
1998	STRUCTURAL REQUIREMENTS OF ANTIBODY COMBINING SITES	5R01AI023909-12	25
1997	POLYCLONAL ANTIBODY LIBRARIES FOR C PARVUM THERAPY	5U01AI040344-02	5

Showing 10 of 25 results. Show All Results

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis Copy PMIDs To Clipboard

Lu Z, Rynkiewicz MJ, Yang CY, Madico G, Perkins HM, Roche MI, Seaton BA, Sharon J. Functional and structural characterization of Francisella tularensis O-antigen antibodies at the low end of antigen reactivity. Monoclon Antib Immunodiagn Immunother. 2014 Aug; 33(4):235-45.View Related Profiles.

Seaton, Barbara
Madico, Guillermo
Sharon, Jacqueline
Rynkiewicz, Michael
Lu, Zhaohua

PMID: 25171003; PMCID: PMC4151076; DOI: 10.1089/mab.2014.0022;

Lu Z, Rynkiewicz MJ, Madico G, Li S, Yang CY, Perkins HM, Sompuram SR, Kodela V, Liu T, Morris T, Wang D, Roche MI, Seaton BA, Sharon J. B-cell epitopes in GroEL of Francisella tularensis. PLoS One. 2014; 9(6):e99847.View Related Profiles.

Seaton, Barbara
Madico, Guillermo
Sharon, Jacqueline
Rynkiewicz, Michael
Lu, Zhaohua

PMID: 24968190; PMCID: PMC4072690; DOI: 10.1371/journal.pone.0099847;

Sharon J, Rynkiewicz MJ, Lu Z, Yang CY. Discovery of protective B-cell epitopes for development of antimicrobial vaccines and antibody therapeutics. Immunology. 2014 May; 142(1):1-23.View Related Profiles.

Sharon, Jacqueline
Rynkiewicz, Michael
Lu, Zhaohua

PMID: 24219801; PMCID: PMC3992043; DOI: 10.1111/imm.12213;

Lu Z, Perkins HM, Sharon J. Antibodies to both terminal and internal B-cell epitopes of Francisella tularensis O-polysaccharide produced by patients with tularemia. Clin Vaccine Immunol. 2014 Feb; 21(2):227-33.View Related Profiles.

Sharon, Jacqueline
Lu, Zhaohua

PMID: 24351753; PMCID: PMC3910946; DOI: 10.1128/CVI.00626-13;

Lu Z, Rynkiewicz MJ, Yang CY, Madico G, Perkins HM, Wang Q, Costello CE, Zaia J, Seaton BA, Sharon J. The binding sites of monoclonal antibodies to the non-reducing end of Francisella tularensis O-antigen accommodate mainly the terminal saccharide. Immunology. 2013 Nov; 140(3):374-89.View Related Profiles.

Seaton, Barbara
Costello, Catherine
Madico, Guillermo
Sharon, Jacqueline
Zaia, Joseph
Rynkiewicz, Michael
Wang, Qi
Lu, Zhaohua

PMID: 23844703; PMCID: PMC3800442; DOI: 10.1111/imm.12150;

Rynkiewicz MJ, Lu Z, Hui JH, Sharon J, Seaton BA. Structural analysis of a protective epitope of the Francisella tularensis O-polysaccharide. Biochemistry. 2012 Jul 17; 51(28):5684-94.View Related Profiles.

Seaton, Barbara
Sharon, Jacqueline
Rynkiewicz, Michael
Lu, Zhaohua

PMID: 22747335; PMCID: PMC3566871; DOI: 10.1021/bi201711m;

Lu Z, Madico G, Roche MI, Wang Q, Hui JH, Perkins HM, Zaia J, Costello CE, Sharon J. Protective B-cell epitopes of Francisella tularensis O-polysaccharide in a mouse model of respiratory tularaemia. Immunology. 2012 Jul; 136(3):352-60.View Related Profiles.

Costello, Catherine
Madico, Guillermo
Sharon, Jacqueline
Zaia, Joseph
Wang, Qi
Lu, Zhaohua

PMID: 22486311; PMCID: PMC3385035; DOI: 10.1111/j.1365-2567.2012.03589.x;

Wang Q, Shi X, Leymarie N, Madico G, Sharon J, Costello CE, Zaia J. A typical preparation of Francisella tularensis O-antigen yields a mixture of three types of saccharides. Biochemistry. 2011 Dec 20; 50(50):10941-50.View Related Profiles.

Costello, Catherine
Madico, Guillermo
Sharon, Jacqueline
Zaia, Joseph
Leymarie, Nancy
Wang, Qi
Shi, Xiaofeng

PMID: 22091710; PMCID: PMC3238095; DOI: 10.1021/bi201450v;

Roche MI, Lu Z, Hui JH, Sharon J. Characterization of monoclonal antibodies to terminal and internal O-antigen epitopes of Francisella tularensis lipopolysaccharide. Hybridoma (Larchmt). 2011 Feb; 30(1):19-28.View Related Profiles.

Sharon, Jacqueline
Lu, Zhaohua

PMID: 21466282; PMCID: PMC3119334; DOI: 10.1089/hyb.2010.0083;

Liebman MA, Roche MI, Williams BR, Kim J, Pageau SC, Sharon J. Antibody treatment of human tumor xenografts elicits active anti-tumor immunity in nude mice. Immunol Lett. 2007 Nov 30; 114(1):16-22. PMID: 17920694; PMCID: PMC2128754; DOI: 10.1016/j.imlet.2007.08.006;

Showing 10 of 55 results. Show More

Lu Z, Roche MI, Hui JH, Unal B, Felgner PL, Gulati S, Madico G, Sharon J. Generation and characterization of hybridoma antibodies for immunotherapy of tularemia. Immunol Lett. 2007 Oct 15; 112(2):92-103.View Related Profiles.

Madico, Guillermo
Sharon, Jacqueline
Lu, Zhaohua

PMID: 17764754; PMCID: PMC2128743; DOI: 10.1016/j.imlet.2007.07.006;

Wiberg FC, Rasmussen SK, Frandsen TP, Rasmussen LK, Tengbjerg K, Coljee VW, Sharon J, Yang CY, Bregenholt S, Nielsen LS, Haurum JS, Tolstrup AB. Production of target-specific recombinant human polyclonal antibodies in mammalian cells. Biotechnol Bioeng. 2006 Jun 5; 94(2):396-405. PMID: 16596663

Sharon J, Liebman MA, Williams BR. Recombinant polyclonal antibodies for cancer therapy. J Cell Biochem. 2005 Oct 1; 96(2):305-13. PMID: 16052504

Liebman MA, Williams BR, Daley KM, Sharon J. Generation and preliminary characterization of an antibody library with preferential reactivity to human colorectal cancer cells as compared to normal human blood cells. Immunol Lett. 2004 Feb 15; 91(2-3):179-88. PMID: 15019288

Chen L, Liebman MA, Teodorescu-Frumosu S, Schnitzler AC, Sharon J. Expression of a prototypic anti-colorectal cancer polyclonal antibody library in mammalian cells. Immunol Lett. 2003 Aug 5; 88(2):135-40. PMID: 12880683

Chen L, Williams BR, Yang CY, Cevallos AM, Bhat N, Ward H, Sharon J. Polyclonal Fab phage display libraries with a high percentage of diverse clones to Cryptosporidium parvum glycoproteins. Int J Parasitol. 2003 Mar; 33(3):281-91. PMID: 12670513

Williams BR, Sompuram SR, Sharon J. Generation of anti-colorectal cancer fab phage display libraries with a high percentage of diverse antigen-reactive clones. Comb Chem High Throughput Screen. 2002 Sep; 5(6):489-99. PMID: 12470278

Williams BR, Sharon J. Polyclonal anti-colorectal cancer Fab phage display library selected in one round using density gradient centrifugation to separate antigen-bound and free phage. Immunol Lett. 2002 Apr 22; 81(2):141-8. PMID: 11852119

Sharon J, Sompuram SR, Yang CY, Williams BR, Sarantopoulos S. Construction of polyclonal antibody libraries using phage display. Methods Mol Biol. 2002; 178:101-12. PMID: 11968479

Sharon J, Sarantopoulos S, Den W, Kao CY, Baecher-Allan CM, Santora KE, Sompuram SR, Petersen-Mahrt S, Williams BR. Recombinant polyclonal antibody libraries. Comb Chem High Throughput Screen. 2000 Jun; 3(3):185-96. PMID: 10903378

Santora KE, Sarantopoulos S, Den W, Petersen-Mahrt S, Sompuram SR, Sharon J. Generation of a polyclonal fab phage display library to the human breast carcinoma cell line BT-20. Comb Chem High Throughput Screen. 2000 Feb; 3(1):51-7. PMID: 10702614

Yao J, Baecher-Allan CM, Sharon J. Serpins identified as cell growth inhibitors in human plasma. Mol Cell Biol Res Commun. 2000 Feb; 3(2):76-81. PMID: 10775503

Baecher-Allan CM, Santora K, Sarantopoulos S, Den W, Sompuram SR, Sharon J, Cevallos AM, Bhat N, Ward H. Generation of a polyclonal Fab phage display library to the protozoan parasite Cryptosporidium parvum. Comb Chem High Throughput Screen. 1999 Dec; 2(6):319-25. PMID: 10644857

Den W, Sompuram SR, Sarantopoulos S, Sharon J. A bidirectional phage display vector for the selection and mass transfer of polyclonal antibody libraries. J Immunol Methods. 1999 Jan 1; 222(1-2):45-57. PMID: 10022371

Gulati S, McQuillen DP, Sharon J, Rice PA. Experimental immunization with a monoclonal anti-idiotope antibody that mimics the Neisseria gonorrhoeae lipooligosaccharide epitope 2C7. J Infect Dis. 1996 Dec; 174(6):1238-48. PMID: 8940214

Sompuram SR, Den W, Sharon J. Analysis of antigen binding and idiotypic expression by antibodies with polyglycine-replaced complementarity-determining regions. J Immunol. 1996 Feb 1; 156(3):1071-81. PMID: 8557981

Sarantopoulos S, Kao CY, Den W, Sharon J. A method for linking VL and VH region genes that allows bulk transfer between vectors for use in generating polyclonal IgG libraries. J Immunol. 1994 Jun 1; 152(11):5344-51. PMID: 8189052

Jacobson BA, Sharon J, Shan H, Shlomchik M, Weigert MG, Marshak-Rothstein A. An isotype switched and somatically mutated rheumatoid factor clone isolated from a MRL-lpr/lpr mouse exhibits limited intraclonal affinity maturation. J Immunol. 1994 May 1; 152(9):4489-99.View Related Profiles.

Marshak-Rothstein, Ann
Sharon, Jacqueline

PMID: 8157964

Kao CY, Sharon J. Chimeric antibodies with anti-dextran-derived complementarity-determining regions and anti-p-azophenylarsonate-derived framework regions. J Immunol. 1993 Aug 15; 151(4):1968-78. PMID: 7688391

Sompuram SR, Sharon J. Verification of a model of a F(ab) complex with phenylarsonate by oligonucleotide-directed mutagenesis. J Immunol. 1993 Mar 1; 150(5):1822-8. PMID: 8436817

Nisonoff A, Oliveira TM, Sharon J. An analysis of idiotype expression in a high-affinity, somatically mutated variant of a germline-encoded anti-p-azobenzenearsonate antibody. Int Immunol. 1993 Jan; 5(1):1-9. PMID: 8443119

Sharon J, Kao CY, Sompuram SR. Oligonucleotide-directed mutagenesis of antibody combining sites. Int Rev Immunol. 1993; 10(2-3):113-27. PMID: 8360579

Rashid A, Auchincloss H, Sharon J. Comparison of GK1.5 and chimeric rat/mouse GK1.5 anti-CD4 antibodies for prolongation of skin allograft survival and suppression of alloantibody production in mice. J Immunol. 1992 Mar 1; 148(5):1382-8.View Related Profiles.

Rashid, Asma
Sharon, Jacqueline

PMID: 1538121

Strong RK, Campbell R, Rose DR, Petsko GA, Sharon J, Margolies MN. Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 1. X-ray crystallography, site-directed mutagenesis, and modeling of the complex with hapten. Biochemistry. 1991 Apr 16; 30(15):3739-48. PMID: 2015229

Strong RK, Petsko GA, Sharon J, Margolies MN. Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 2. Structural basis of hapten binding and idiotypy. Biochemistry. 1991 Apr 16; 30(15):3749-57. PMID: 2015230

Fish S, Fleming M, Sharon J, Manser T. Different epitope structures select distinct mutant forms of an antibody variable region for expression during the immune response. J Exp Med. 1991 Mar 1; 173(3):665-72. PMID: 1705280; PMCID: PMC2118828

Parhami-Seren B, Wysocki LJ, Margolies MN, Sharon J. Clustered H chain somatic mutations shared by anti-p-azophenylarsonate antibodies confer enhanced affinity and ablate the cross-reactive idiotype. J Immunol. 1990 Oct 1; 145(7):2340-6. PMID: 2118935

Sharon J. Structural characterization of idiotopes by using antibody variants generated by site-directed mutagenesis. J Immunol. 1990 Jun 15; 144(12):4863-9. PMID: 2351832

Sharon J. Structural correlates of high antibody affinity: three engineered amino acid substitutions can increase the affinity of an anti-p-azophenylarsonate antibody 200-fold. Proc Natl Acad Sci U S A. 1990 Jun; 87(12):4814-7. PMID: 2352950; PMCID: PMC54208

Parhami-Seren B, Sharon J, Margolies MN. Structural characterization of H chain-associated idiotopes of anti-p-azophenylarsonate monoclonal antibodies. J Immunol. 1990 Jun 1; 144(11):4426-33. PMID: 2111347

Sharon J, Gefter ML, Wysocki LJ, Margolies MN. Recurrent somatic mutations in mouse antibodies to p-azophenylarsonate increase affinity for hapten. J Immunol. 1989 Jan 15; 142(2):596-601. PMID: 2911012

Sharon J. The invariant tryptophan in an H chain V region is not essential to antibody binding. J Immunol. 1988 Apr 15; 140(8):2666-9. PMID: 3128602

Sharon J, Gefter ML, Manser T, Ptashne M. Site-directed mutagenesis of an invariant amino acid residue at the variable-diversity segments junction of an antibody. Proc Natl Acad Sci U S A. 1986 Apr; 83(8):2628-31. PMID: 3085101; PMCID: PMC323352

Wims LA, Sharon J, Newman B, Kabat EA, Morrison SL. C57BL/6 x BALB/c hybridomas produce IgA which assembles into molecules with covalent bonds between heavy chains (H) and light chains (L), and into molecules lacking covalent bonds between H and L. Mol Immunol. 1985 Dec; 22(12):1379-86. PMID: 3938526

Sharon J, Gefter ML, Manser T, Morrison SL, Oi VT, Ptashne M. Expression of a VHC kappa chimaeric protein in mouse myeloma cells. Nature. 1984 May 24-30; 309(5966):364-7. PMID: 6328310

Berger CL, Morrison S, Chu A, Patterson J, Estabrook A, Takezaki S, Sharon J, Warburton D, Irigoyen O, Edelson RL. Diagnosis of cutaneous T cell lymphoma by use of monoclonal antibodies reactive with tumor-associated antigens. J Clin Invest. 1982 Dec; 70(6):1205-15. PMID: 6217214; PMCID: PMC370337

Sharon J, Kabat EA, Morrison SL. Association constants of hybridoma antibodies specific for alpha (1 leads to 6) linked dextran determined by affinity electrophoresis. Mol Immunol. 1982 Mar; 19(3):389-97. PMID: 6178962

Sharon J, Kabat EA, Morrison SL. Immunochemical characterization of binding sites of hybridoma antibodies specific for alpha (1 leads to 6) linked dextran. Mol Immunol. 1982 Mar; 19(3):375-88. PMID: 6178961

Sharon J, D'Hoostelaere L, Potter M, Kabat EA, Morrison SL. A cross-reactive idiotype, QUPC52 IdX, present on most but not all anti-alpha (1 replaced by 6) dextran-specific IgM and IgA hybridoma antibodies with combining sites of different sizes. J Immunol. 1982 Jan; 128(1):498-500. PMID: 6172490

Matsuuchi L, Sharon J, Morrison SL. An analysis of heavy chain glycopeptides of hybridoma antibodies: correlation between antibody specificity and sialic acid content. J Immunol. 1981 Nov; 127(5):2188-90. PMID: 6795268

Sharon J, Kabat EA, Morrison SL. Studies on mouse hybridomas secreting IgM or IgA antibodies to alpha(1 to 6)-linked dextran. Mol Immunol. 1981 Sep; 18(9):831-46. PMID: 6175897

Sharon J, Morrison SL, Kabat EA. Formation of hybridoma clones in soft agarose: effect of pH and of medium. Somatic Cell Genet. 1980 May; 6(3):435-41. PMID: 7190739

Sharon J, Morrison SL, Kabat EA. Detection of specific hybridoma clones by replica immunoadsorption of their secreted antibodies. Proc Natl Acad Sci U S A. 1979 Mar; 76(3):1420-4. PMID: 286325; PMCID: PMC383263

Givol D, Sharon J, Hochman J, Gavish M, Inbar D, Pecht I, Steinberg IZ, Schlessinger J. Folding, association, and interactions of domains in the antibody molecule. Cold Spring Harb Symp Quant Biol. 1977; 41 Pt 2:667-75. PMID: 268251

Sharon J, Givol D. Preparation of Fv fragment from the mouse myeloma XRPC-25 immunoglobulin possessing anti-dinitrophenyl activity. Biochemistry. 1976 Apr 6; 15(7):1591-4. PMID: 4096

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 52 publications over 26 distinct years, with a maximum of 4 publications in 1982 and 1990 and 1993

Bar chart showing 52 publications over 26 distinct years, with a maximum of 4 publications in 1982 and 1990 and 1993

Year	Publications
1980	1
1981	2
1982	4
1984	1
1985	1
1986	1
1988	1
1989	1
1990	4
1991	3
1992	1
1993	4
1994	2
1996	2
1999	2
2000	3
2002	3
2003	2
2004	1
2005	1
2006	1
2007	2
2011	2
2012	2
2013	2
2014	3

In addition to these self-described keywords below, a list of MeSH based concepts is available here.

immunology
infectious disease

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