-
-
-
-
-
Komatsu T, Salih E, Helmerhorst EJ, Offner GD, Oppenheim FG. Influence of histatin 5 on Candida albicans mitochondrial protein expression assessed by quantitative mass spectrometry. J Proteome Res. 2011 Feb 4; 10(2):646-55.View Related Profiles. PMID: 21080726; PMCID: PMC3033980; DOI: 10.1021/pr100861k;
-
Helmerhorst EJ, Traboulsi G, Salih E, Oppenheim FG. Mass spectrometric identification of key proteolytic cleavage sites in statherin affecting mineral homeostasis and bacterial binding domains. J Proteome Res. 2010 Oct 1; 9(10):5413-21.View Related Profiles. PMID: 20731414; PMCID: PMC2948583; DOI: 10.1021/pr100653r;
-
Zhou HY, Salih E, Glimcher MJ. The isolation and characterization of glycosylated phosphoproteins from herring fish bones. J Biol Chem. 2010 Nov 12; 285(46):36170-8. PMID: 20833721; PMCID: PMC2975239; DOI: 10.1074/jbc.M110.146910;
-
-
-
-
-
Sun X, Salih E, Oppenheim FG, Helmerhorst EJ. Kinetics of histatin proteolysis in whole saliva and the effect on bioactive domains with metal-binding, antifungal, and wound-healing properties. FASEB J. 2009 Aug; 23(8):2691-701.View Related Profiles. PMID: 19339663; PMCID: PMC2717783; DOI: 10.1096/fj.09-131045;
-
-
-
Saad FA, Salih E, Glimcher MJ. Identification of osteopontin phosphorylation sites involved in bone remodeling and inhibition of pathological calcification. J Cell Biochem. 2008 Feb 15; 103(3):852-6. PMID: 17615552; PMCID: PMC2744143
-
Siqueira WL, Helmerhorst EJ, Zhang W, Salih E, Oppenheim FG. Acquired enamel pellicle and its potential role in oral diagnostics. Ann N Y Acad Sci. 2007 Mar; 1098:504-9.View Related Profiles. PMID: 17435160
-
Oppenheim FG, Salih E, Siqueira WL, Zhang W, Helmerhorst EJ. Salivary proteome and its genetic polymorphisms. Ann N Y Acad Sci. 2007 Mar; 1098:22-50.View Related Profiles. PMID: 17303824
-
Wang J, Zhou HY, Salih E, Xu L, Wunderlich L, Gu X, Hofstaetter JG, Torres M, Glimcher MJ. Site-specific in vivo calcification and osteogenesis stimulated by bone sialoprotein. Calcif Tissue Int. 2006 Sep; 79(3):179-89. PMID: 16969594
-
Salih E. Phosphoproteomics by mass spectrometry and classical protein chemistry approaches. Mass Spectrom Rev. 2005 Nov-Dec; 24(6):828-46. PMID: 15538747
-
Saad FA, Salih E, Wunderlich L, Flückiger R, Glimcher MJ. Prokaryotic expression of bone sialoprotein and identification of casein kinase II phosphorylation sites. Biochem Biophys Res Commun. 2005 Jul 29; 333(2):443-7. PMID: 15950178
-
Mah J, Hung J, Wang J, Salih E. The efficacy of various alloplastic bone grafts on the healing of rat calvarial defects. Eur J Orthod. 2004 Oct; 26(5):475-82. PMID: 15536835
-
O'Toole GC, Salih E, Gallagher C, FitzPatrick D, O'Higgins N, O'Rourke SK. Bone sialoprotein-coated femoral implants are osteoinductive but mechanically compromised. J Orthop Res. 2004 May; 22(3):641-6. PMID: 15099646
-
Salih E, Flückiger R. Complete topographical distribution of both the in vivo and in vitro phosphorylation sites of bone sialoprotein and their biological implications. J Biol Chem. 2004 May 7; 279(19):19808-15. PMID: 15004024
-
Tartaix PH, Doulaverakis M, George A, Fisher LW, Butler WT, Qin C, Salih E, Tan M, Fujimoto Y, Spevak L, Boskey AL. In vitro effects of dentin matrix protein-1 on hydroxyapatite formation provide insights into in vivo functions. J Biol Chem. 2004 Apr 30; 279(18):18115-20. PMID: 14769788
-
Goldhaber, P., Rabadjija, L., Fluckiger, R., Salih, E., Bernard, G. Effects of ascorbic acid on formation and differentiation of bone cell aggregates and their ability to heal bone resorption defects by new osteoid formation in neonatal mouse calvaria grown in serum-free organ cultures. Biological mechanisms of tooth eruption, resorption, and implants. Birmingham AL: EBSCO Media. 2004; 179-195.
-
Wang J. Zhou, H-Y, Salih, E., Xu, L., Hofstaetter, J.G. and Glimcher MJ. Bone sialoprotein elicits biomineralization and ossification in a bone defect model. The chemistry and biology of mineralized tissues University of Toronto Press. 2004; 139-142.
-
Curtin, P.; McHugh, K.; Goldhaber, P.; Flückiger, R.; Salih, E. Role of bone sialoprotein phosphorylation in bone resorption using calvarial organ and osteoclast cultures. The chemistry and biology of mineralized tissues. University of Toronto Press. 2004; 73-76.
-
Salih, E. . Emergence of phosphoproteomics through combination of mass spectrometry and classical protein chemistry approaches. he chemistry and biology of mineralized tissues University of Toronto Pres. 2004; 208-211.
-
Salih E. Synthesis of a radioactive thiol reagent, 1-S-[3H]carboxymethyl-dithiothreitol: identification of the phosphorylation sites by N-terminal peptide sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Biochem. 2003 Aug 1; 319(1):143-58. PMID: 12842118
-
Choi EK, Miller JS, Zaidi NF, Salih E, Buxbaum JD, Wasco W. Phosphorylation of calsenilin at Ser63 regulates its cleavage by caspase-3. Mol Cell Neurosci. 2003 Jul; 23(3):495-506. PMID: 12837631
-
Carvalho RS, Kostenuik PJ, Salih E, Bumann A, Gerstenfeld LC. Selective adhesion of osteoblastic cells to different integrin ligands induces osteopontin gene expression. Matrix Biol. 2003 May; 22(3):241-9.View Related Profiles. PMID: 12853034
-
Goldberg M, Six N, Decup F, Lasfargues JJ, Salih E, Tompkins K, Veis A. Bioactive molecules and the future of pulp therapy. Am J Dent. 2003 Feb; 16(1):66-76. PMID: 12744417
-
Salih E. In vivo and in vitro phosphorylation regions of bone sialoprotein. Connect Tissue Res. 2003; 44 Suppl 1:223-9. PMID: 12952202
-
Salih E, Wang J, Mah J, Fluckiger R. Natural variation in the extent of phosphorylation of bone phosphoproteins as a function of in vivo new bone formation induced by demineralized bone matrix in soft tissue and bony environments. Biochem J. 2002 Jun 1; 364(Pt 2):465-74. PMID: 12023890; PMCID: PMC1222592
-
Goldberg M, Six N, Decup F, Bourd K, Palmier K, Salih E, Veis A, Lasfargues JJ. [Mineralization of the dental pulp: contributions of tissue engineering to tomorrow's therapeutics in odontology]. Pathol Biol (Paris). 2002 Apr; 50(3):194-203. PMID: 11980334
-
Six N, Decup F, Lasfargues JJ, Salih E, Goldberg M. Osteogenic proteins (bone sialoprotein and bone morphogenetic protein-7) and dental pulp mineralization. J Mater Sci Mater Med. 2002 Feb; 13(2):225-32. PMID: 15348647
-
Goldberg M, Six N, Decup F, Buch D, Soheili Majd E, Lasfargues JJ, Salih E, Stanislawski L. Application of bioactive molecules in pulp-capping situations. Adv Dent Res. 2001 Aug; 15:91-5. PMID: 12640750
-
Kim Y, Takanori T, Elovic A, Shintani S, Mihara M, Salih E, Kohno Y, Chin B-R, Patel V, Wong D, Todd R. Murin doc-1 cDNA cloning, sequencing and expression in normal adult tissues. Int. J. Oral Biol. 2001; 87-91.
-
Decup F, Six N, Palmier B, Buch D, Lasfargues JJ, Salih E, Goldberg M. Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar. Clin Oral Investig. 2000 Jun; 4(2):110-9. PMID: 11218498
-
Kennedy JG, O'Grady P, McCarthy DR, Johnson SJ, Hynes D, Walsh M, McManus FM, Salih E, Gouverneur M, Fitzpatrick J. An investigation into the role of oxygen free radical scavengers in preventing polymethylmethacrylate-induced necrosis in an osteoblast cell culture. Orthopedics. 2000 May; 23(5):481-5. PMID: 10825116
-
Kennedy J, Harty JA, Gouverneur M, Salih E, Walsh MG, McManus F, and Fitzpatrick J. The role of collagen type IV angiogenic factor in reversing osteonecrosis in rabbit femoral head model. J.Orthopedic Surg. & Traum. 2000; 5(1):1-10.
-
Zhou H-Y,Wang J, Glimcher MJ, *Salih E. Osteometrin. The chemistry and biology of mineralized tissues: Rosemont, IL: Amer Acad Orthopaedic Surg Press. 2000; 185-188.
-
Salih E, Huang J, Gouverneur M, Glimcher M. Novel phosphoproteins and protein kinases of tooth enamel: Novel methods of determining covalently-bound phosphates in phosphoproteins. The chemistry and biology of mineralized tissues: Rosemont, IL: Amer Acad Orthopaedic Surg Press. 2000; 191-198.
-
Wang J, Glimcher MJ, Mah J, Zhou HY, Salih E. Expression of bone microsomal casein kinase II, bone sialoprotein, and osteopontin during the repair of calvarial defects. Bone. 1998 Jun; 22(6):621-8. PMID: 9626400
-
Zhou HY, Salih E, Glimcher MJ. Isolation of a novel bone glycosylated phosphoprotein with disulphide cross-links to osteonectin. Biochem J. 1998 Mar 15; 330 ( Pt 3):1423-31. PMID: 9494116; PMCID: PMC1219292
-
Salih E, Huang JC, Strawich E, Gouverneur M, Glimcher MJ. Enamel specific protein kinases and state of phosphorylation of purified amelogenins. Connect Tissue Res. 1998; 38(1-4):225-35; discussion 241-6. PMID: 11063030
-
Salih E, Huang J, Gouverneur M, Ly D, Mah J, Glimcher MJ. . Protein kinases that phosphorylated extracellular matrix phosphoproteins of mineralizing tissues: Novel enamel protein kinase. Biological mechanisms of tooth eruption, resorption, and implants. 1998; 133-147.
-
Wang J, Mah J, Glimcher MJ, Salih E. Biochemical changes during new bone formation in the calvarial and subcutaneous tissue environments. Biological mechanisms of tooth eruption, resorption, and implants. Birmingham AL: EBSCO Media. 1998; 123-132.
-
Salih E, Ashkar S, Gerstenfeld LC, Glimcher MJ. Identification of the phosphorylated sites of metabolically 32P-labeled osteopontin from cultured chicken osteoblasts. J Biol Chem. 1997 May 23; 272(21):13966-73.View Related Profiles. PMID: 9153260
-
Salih E, Ashkar S, Gerstenfeld LC, Glimcher MJ. Protein kinases of cultured osteoblasts: selectivity for the extracellular matrix proteins of bone and their catalytic competence for osteopontin. J Bone Miner Res. 1996 Oct; 11(10):1461-73.View Related Profiles. PMID: 8889846
-
Salih E, Zhou HY, Glimcher MJ. Phosphorylation of purified bovine bone sialoprotein and osteopontin by protein kinases. J Biol Chem. 1996 Jul 12; 271(28):16897-905. PMID: 8663267
-
Salih E. . Rate enhancement in acetylcholinesterase catalyzed reactions through coupling of increased collision density and correct orientation. Proc Chem Defence Biosc. 1996; (1):245-258.
-
Salih E, Ashkar S, Zhou HY, Gerstenfeld L, Glimcher MJ. Protein kinases of cultured chicken osteoblasts that phosphorylate extracellular bone proteins. Connect Tissue Res. 1996; 35(1-4):207-13.View Related Profiles. PMID: 9084659
-
Salih E, Chishti B, Vicedomine P, Cohen SG, Chiara DC, Cohen JB. . Inactivation and labeling by [14C] Phenocyl Bromide of Acetylcoholinesterase, from E. Electricus at His-550 in a peripheral site and from T.Nobiliana at Cys-231 and Ser-226 in the active site and His-550. Proc Chem Defence. 1996; (1):259-271.
-
Gerstenfeld LC, Uporova T, Ashkar S, Salih E, Gotoh Y, McKee MD, Nanci A, Glimcher MJ. Regulation of avian osteopontin pre- and posttranscriptional expression in skeletal tissues. Ann N Y Acad Sci. 1995 Apr 21; 760:67-82.View Related Profiles. PMID: 7785927
-
Salih E, Ashkar S, Gerstenfeld LC, Glimcher MJ. Identification of the in vivo phosphorylated sites of secreted osteopontin from cultured chicken osteoblasts. Ann N Y Acad Sci. 1995 Apr 21; 760:357-60.View Related Profiles. PMID: 7785916
-
Ashkar S, Schaffer JL, Salih E, Gerstenfeld LC, Glimcher MJ. Phosphorylation of osteopontin by Golgi kinases. Ann N Y Acad Sci. 1995 Apr 21; 760:296-8.View Related Profiles. PMID: 7785903
-
Gotoh Y, Salih E, Glimcher MJ, Gerstenfeld LC. Characterization of the major non-collagenous proteins of chicken bone: identification of a novel 60 kDa non-collagenous phosphoprotein. Biochem Biophys Res Commun. 1995 Mar 17; 208(2):863-70.View Related Profiles. PMID: 7695644
-
Salih E, Chishti SB, Vicedomine P, Cohen SG, Chiara DC, Cohen JB. Active-site peptides of acetylcholinesterase of Electrophorus electricus: labelling of His-440 by 1-bromo-[2-14C]pinacolone and Ser-200 by tritiated diisopropyl fluorophosphate. Biochim Biophys Acta. 1994 Oct 19; 1208(2):324-31. PMID: 7947965
-
Salih E, Howard S, Chishti SB, Cohen SG, Liu WS, Cohen JB. Labeling of cysteine 231 in acetylcholinesterase from Torpedo nobiliana by the active-site directed reagent, 1-bromo-2-[14C] pinacolone. Effects of 2,2'-dipyridyl disulfide and other sulfhydryl reagents. J Biol Chem. 1993 Jan 5; 268(1):245-51. PMID: 8416933
-
Salih E, Chishti SB, Vicedomine P, Cohen SG, Liu WS, Cohen JB. Labelling by l-bromo-2-[14C] pinacolone of His-440 and Trp-435 in acetylcholinesterase from Electrophorus electricus. Proc Chem Defence Biosc. 1993; (2):759-767.
-
Salih E. Catalysis by acetylcholinesterase in two-hydronic-reactive states. Integrity of deuterium oxide effects and hydron inventories. Biochem J. 1992 Jul 15; 285 ( Pt 2):451-60. PMID: 1322133; PMCID: PMC1132809
-
Topham CM, Salih E, Frazao C, Kowlessur D, Overington JP, Thomas M, Brocklehurst SM, Patel M, Thomas EW, Brocklehurst K. Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase omega. Three-dimensional structure of papaya proteinase omega deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysis. Biochem J. 1991 Nov 15; 280 ( Pt 1):79-92. PMID: 1741760; PMCID: PMC1130603
-
Salih E. Two-hydronic-reactive states of acetylcholinesterase, mechanistically relevant acid-base catalyst of pKa 6.5 and a modulatory group of pKa 5.5. Biochim Biophys Acta. 1991 Jan 23; 1073(1):183-94. PMID: 1991134
-
Cohen SG, Chishti SB, Bell DA, Howard SI, Salih E, Cohen JB. General occurrence of binding to acetylcholinesterase-substrate complex in noncompetitive inhibition and in inhibition by substrate. Biochim Biophys Acta. 1991 Jan 8; 1076(1):112-22. PMID: 1986784
-
Salih E, Howard S, Chishti SB, Cohen SG, Liu WS, Cohen JB. . Labelling of cysteine-231 by l-bromo-2-14C pinacolone in the active site of acetylcholinesterase from torpedo nobiliana. Proc Chem Defence Biosc. 1991; (1):603-606.
-
Topham CM, Overington J, O'driscoll M, Salih E, Thomas M, Thomas EW, Brocklehurst K. Three-dimensional structure of a B-type chymopapain. Biochem Soc Trans. 1990 Oct; 18(5):933-4. PMID: 2083745
-
French H, Williams R, Salih E, Kowlessur D, Brocklehurst K. Studies on streptococcal proteinase. Biochem Soc Trans. 1990 Aug; 18(4):593-4. PMID: 2276454
-
Cohen SG, Salih E, Solomon M, Howard S, Chishti SB, Cohen JB. Reactions of 1-bromo-2-[14C]pinacolone with acetylcholinesterase from Torpedo nobiliana. Effects of 5-trimethylammonio-2-pentanone and diisopropyl fluorophosphate. Biochim Biophys Acta. 1989 Aug 31; 997(3):167-75. PMID: 2765553
-
Cohen SG, Salih E, Solomon M, Howard S, Chishti SB, Cohen JB, Broomfield C. . Reaction of 1-bromo-2-14C-pinacolone with acetylcholinesterase from torpedo nobiliana. Effects of 5-trimethyl ammonio-2-pentanone, diisopropyl fluorophosphate and soman. Proc Chem Defence Biosc. 1989; (1):497-500.
-
Brocklehurst K, Brocklehurst SM, Kowlessur D, O'Driscoll M, Patel G, Salih E, Templeton W, Thomas E, Topham CM, Willenbrock F. Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics. Biochem J. 1988 Dec 1; 256(2):543-58. PMID: 3223929; PMCID: PMC1135444
-
Salih E, Malthouse JP, Kowlessur D, Jarvis M, O'Driscoll M, Brocklehurst K. Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies targeted on the catalytic-site thiol group and its immediate microenvironment. Biochem J. 1987 Oct 1; 247(1):181-93. PMID: 2825655; PMCID: PMC1148386
-
Brocklehurst K, Kowlessur D, O'Driscoll M, Patel G, Quenby S, Salih E, Templeton W, Thomas EW, Willenbrock F. Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding. Biochem J. 1987 May 15; 244(1):173-81. PMID: 3663111; PMCID: PMC1147969
-
Brocklehurst K, Willenbrock F, Salih E. Chapter: Cysteine proteinases. In:Neuberger, A. and Brocklehurst K., editors. Hydrolytic Enzymes: New Comprehensive Biochemistry. Elsevier. Amsterdam/New York/Oxford. 1987; 39-158.
-
Baines BS, Brocklehurst K, McKee R, O’Driscoll M, Salih E, Smith H. . Identification and characterization of chymopapain A and B from dried and fresh latex of carica papaya. Biochem Soc Trans. 1986; (14):1226-1227.
-
Baines BS, Brocklehurst K, Carey PR, Jarvis M, Salih E, Storer AC. Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives. Biochem J. 1986 Jan 1; 233(1):119-29. PMID: 3513753; PMCID: PMC1152993
-
Brocklehurst K, Salih E, McKee R, Smith H. Fresh non-fruit latex of Carica papaya contains papain, multiple forms of chymopapain A and papaya proteinase omega. Biochem J. 1985 Jun 1; 228(2):525-7. PMID: 4015629; PMCID: PMC1145013
-
Brocklehurst K, McKee R, Salih E, Smith H. . Evidence from two-protonic-state reactivity probe kinetics that chymopapain in fresh non-fruit latex of carica papaya consists of multiple forms of chymopapain A. The value of catalytic site characteristics in the identification, classification and characterization of the papaya cysteine proteinases papain, the chymopapains and papaya proteinase II. J Protein Chem. 1985; (4):103-127.
-
Brocklehurst K, Carey PR, Lee HH, Salih E, Storer AC. Comparative resonance Raman spectroscopic and kinetic studies of acyl-enzymes involving papain, actinidin and papaya peptidase II. Biochem J. 1984 Nov 1; 223(3):649-57. PMID: 6391467; PMCID: PMC1144348
-
Brocklehurst K, Baines BS, Salih E, Hatzoulis C. 'Chymopapain S' is chymopapain A. Biochem J. 1984 Jul 15; 221(2):553-4. PMID: 6383349; PMCID: PMC1144074
-
Brocklehurst K, Salih E, Lodwig TS. Differences between the electric fields of the catalytic sites of papain and actinidin detected by using the thiol-located nitrobenzofurazan label as a spectroscopic reporter group. Biochem J. 1984 Jun 1; 220(2):609-12. PMID: 6378189; PMCID: PMC1153668
-
Salih E, Brocklehurst K. Investigation of the catalytic site of actinidin by using benzofuroxan as a reactivity probe with selectivity for the thiolate-imidazolium ion-pair systems of cysteine proteinases. Evidence that the reaction of the ion-pair of actinidin (pKI 3.0, pKII 9.6) is modulated by the state of ionization of a group associated with a molecular pKa of 5.5. Biochem J. 1983 Sep 1; 213(3):713-8. PMID: 6311173; PMCID: PMC1152187
-
Brocklehurst K, Salih E. A re-evaluation of the nomenclature of the cysteine proteinases of Carica papaya and a rational basis for their identification. Biochem J. 1983 Aug 1; 213(2):559-60. PMID: 6351846; PMCID: PMC1152164
-
Brocklehurst K, Willenbrock SJ, Salih E. Effects of conformational selectivity and of overlapping kinetically influential ionizations on the characteristics of pH-dependent enzyme kinetics. Implications of free-enzyme pKa variability in reactions of papain for its catalytic mechanism. Biochem J. 1983 Jun 1; 211(3):701-8. PMID: 6309137; PMCID: PMC1154417
-
Salih E, Willenbrock F, Baines BS, Brocklehurst K. Benzofuroxane as a reactivity probe for the study of disposition of nucleophilic and acid-base groups in enzyme active centers. Biochem Soc Trans. 1982; (10):217-218.