Keywords
Last Name

Paul Toselli, MD, PhD

TitleAssociate Professor Emeritus
InstitutionBoston University School of Medicine
DepartmentBiochemistry
Address72 E. Concord St Silvio Conte (K)
Boston MA 02118
Phone(617) 638-4050
Other Positions
TitleGraduate Faculty (Primary Mentor of Grad Students)
InstitutionBoston University School of Medicine, Graduate Medical Sciences

InstitutionBoston Medical Center

 Research Expertise & Professional Interests
During my time at BUSM, I have taught medical students in anatomy, physiology, and pathology courses. Over the past seven years, I have participated in teaching GMS BI 555/755, a biochemistry course open to graduate students from all basic science departments. In this course, I teach fundamental concepts and practical applications of light and electron microscopy for experimental research. My instruction ranges from classical histological techniques perfected in the nineteenth century to the most recent developments in immunohistochemistry and histomorphology, including TUNEL assays and in situ hybridization. I teach the students the concepts behind each technique and its application to experimental questions they are likely to face, and also give them the tools to make their own critical evaluation of data generated by these methods.
In addition to formal lectures and associated classroom teaching, I have provided individualized instruction to graduate students, postdocs, technicians, undergraduates, special students, fellow faculty members, and visiting scientists in my laboratory. Over the years there have been literally hundreds of such instances, from brief consultations of few minutes in duration to collaborative efforts spanning years. I have given freely of my time to all who have entered my lab with experimental questions that could be answered by the anatomical and related techniques at my disposal. I have concentrated my efforts on helping graduate students and postdocs in the Biochemistry department. My help has included everything from tissue preparation to photography for publication. I have spent an enormous amount of time and effort teaching people how to fix, embed, section, and image tissue from experimental animals, cultured cells, and human samples. I have taught students at various levels how best to use fluorescent, phase-contrast, and polarizing light microscopes, and this has extended to electron microscopy, both transmission and scanning. I developed and perfected in situ hybridization techniques with one set of students and faculty members, and then made my expertise in this technology available to subsequent lab groups who needed it. I have made critical contributions of this nature to the thesis work of a dozen doctoral candidates in the Biochemistry department alone in the past seven years. I have also taught, trained, and guided postdocs, master students, students from CityLab, and technicians.
In addition, I employed the electron microscope to detect and localize beta-galactosidase expression in lung tissue, vascular tissue, and platelets. The introduction of the electron microscope to precisely localize beta-galactosidase expression demonstrates a highly innovative scientific approach that I introduced to the Biochemistry Department faculty and to Ph.D. candidates.

 Self-Described Keywords
 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Zhao Y, Kumbrink J, Lin BT, Bouton AH, Yang S, Toselli PA, Kirsch KH. Expression of a phosphorylated substrate domain of p130Cas promotes PyMT-induced c-Src-dependent murine breast cancer progression. Carcinogenesis. 2013 Dec; 34(12):2880-90.
    View in: PubMed
  2. Biasini E, Unterberger U, Solomon IH, Massignan T, Senatore A, Bian H, Voigtlaender T, Bowman FP, Bonetto V, Chiesa R, Luebke J, Toselli P, Harris DA. A mutant prion protein sensitizes neurons to glutamate-induced excitotoxicity. J Neurosci. 2013 Feb 6; 33(6):2408-18.
    View in: PubMed
  3. Bergethon PR, Kindler DD, Hallock K, Blease S, Toselli P. Continuous exposure to low amplitude extremely low frequency electrical fields characterizing the vascular streaming potential alters elastin accumulation in vascular smooth muscle cells. Bioelectromagnetics. 2013 Jul; 34(5):358-65.
    View in: PubMed
  4. Gao S, Zhou J, Zhao Y, Toselli P, Li W. Hypoxia-response element (HRE)-directed transcriptional regulation of the rat lysyl oxidase gene in response to cobalt and cadmium. Toxicol Sci. 2013 Apr; 132(2):379-89.
    View in: PubMed
  5. Iskratsch T, Reijntjes S, Dwyer J, Toselli P, Dégano IR, Dominguez I, Ehler E. Two distinct phosphorylation events govern the function of muscle FHOD3. Cell Mol Life Sci. 2013 Mar; 70(5):893-908.
    View in: PubMed
  6. Yu J, Taylor L, Rich C, Toselli P, Stone P, Green D, Warburton R, Hill N, Goldstein R, Polgar P. Transgenic expression of an altered angiotensin type I AT1 receptor resulting in marked modulation of vascular type I collagen. J Cell Physiol. 2012 May; 227(5):2013-21.
    View in: PubMed
  7. Zhao Y, Toselli P, Li W. Microtubules as a critical target for arsenic toxicity in lung cells in vitro and in vivo. Int J Environ Res Public Health. 2012 Feb; 9(2):474-95.
    View in: PubMed
  8. Dominguez I, Degano IR, Chea K, Cha J, Toselli P, Seldin DC. CK2a is essential for embryonic morphogenesis. Mol Cell Biochem. 2011 Oct; 356(1-2):209-16.
    View in: PubMed
  9. Yang D, Chen H, Koupenova M, Carroll SH, Eliades A, Freedman JE, Toselli P, Ravid K. A new role for the A2b adenosine receptor in regulating platelet function. J Thromb Haemost. 2010 Apr; 8(4):817-27.
    View in: PubMed
  10. Zhao Y, Chen L, Gao S, Toselli P, Stone P, Li W. The critical role of the cellular thiol homeostasis in cadmium perturbation of the lung extracellular matrix. Toxicology. 2010 Jan 12; 267(1-3):60-9.
    View in: PubMed
  11. Near RI, Smith RS, Toselli PA, Freddo TF, Bloom AB, Vanden Borre P, Seldin DC, Lerner A. Loss of AND-34/BCAR3 expression in mice results in rupture of the adult lens. Mol Vis. 2009; 15:685-99.
    View in: PubMed
  12. Seldin DC, Lou DY, Toselli P, Landesman-Bollag E, Dominguez I. Gene targeting of CK2 catalytic subunits. Mol Cell Biochem. 2008 Sep; 316(1-2):141-7.
    View in: PubMed
  13. Lou DY, Dominguez I, Toselli P, Landesman-Bollag E, O'Brien C, Seldin DC. The alpha catalytic subunit of protein kinase CK2 is required for mouse embryonic development. Mol Cell Biol. 2008 Jan; 28(1):131-9.
    View in: PubMed
  14. Armagan A, Hatsushi K, Toselli P. The effects of testosterone deficiency on the structural integrity of the penile dorsal nerve in the rat. Int J Impot Res. 2008 Jan-Feb; 20(1):73-8.
    View in: PubMed
  15. Gaidos G, Soni S, Oswald DJ, Toselli PA, Kirsch KH. Structure and function analysis of the CMS/CIN85 protein family identifies actin-bundling properties and heterotypic-complex formation. J Cell Sci. 2007 Jul 15; 120(Pt 14):2366-77.
    View in: PubMed
  16. Gao S, Zhao Y, Kong L, Toselli P, Chou IN, Stone P, Li W. Cloning and characterization of the rat lysyl oxidase gene promoter: identification of core promoter elements and functional nuclear factor I-binding sites. J Biol Chem. 2007 Aug 31; 282(35):25322-37.
    View in: PubMed
  17. Chou DK, Zhao Y, Gao S, Chou IN, Toselli P, Stone P, Li W. Perturbation of copper (Cu) homeostasis and expression of Cu-binding proteins in cadmium-resistant lung fibroblasts. Toxicol Sci. 2007 Sep; 99(1):267-76.
    View in: PubMed
  18. Huang Z, Yu J, Toselli P, Bhawan J, Sudireddy V, Taylor L, Polgar P. Angiotensin II type 1 and bradykinin B2 receptors expressed in early stage epithelial cells derived from human embryonic stem cells. J Cell Physiol. 2007 Jun; 211(3):816-25.
    View in: PubMed
  19. Lawrence R, Yabe T, Hajmohammadi S, Rhodes J, McNeely M, Liu J, Lamperti ED, Toselli PA, Lech M, Spear PG, Rosenberg RD, Shworak NW. The principal neuronal gD-type 3-O-sulfotransferases and their products in central and peripheral nervous system tissues. Matrix Biol. 2007 Jul; 26(6):442-55.
    View in: PubMed
  20. Yang D, Zhang Y, Nguyen HG, Koupenova M, Chauhan AK, Makitalo M, Jones MR, St Hilaire C, Seldin DC, Toselli P, Lamperti E, Schreiber BM, Gavras H, Wagner DD, Ravid K. The A2B adenosine receptor protects against inflammation and excessive vascular adhesion. J Clin Invest. 2006 Jul; 116(7):1913-23.
    View in: PubMed
  21. DiCamillo SJ, Yang S, Panchenko MV, Toselli PA, Naggar EF, Rich CB, Stone PJ, Nugent MA, Panchenko MP. Neutrophil elastase-initiated EGFR/MEK/ERK signaling counteracts stabilizing effect of autocrine TGF-beta on tropoelastin mRNA in lung fibroblasts. Am J Physiol Lung Cell Mol Physiol. 2006 Aug; 291(2):L232-43.
    View in: PubMed
  22. Zhao Y, Gao S, Chou IN, Toselli P, Stone P, Li W. Inhibition of the expression of lysyl oxidase and its substrates in cadmium-resistant rat fetal lung fibroblasts. Toxicol Sci. 2006 Apr; 90(2):478-89.
    View in: PubMed
  23. Gao S, Chen K, Zhao Y, Rich CB, Chen L, Li SJ, Toselli P, Stone P, Li W. Transcriptional and posttranscriptional inhibition of lysyl oxidase expression by cigarette smoke condensate in cultured rat fetal lung fibroblasts. Toxicol Sci. 2005 Sep; 87(1):197-203.
    View in: PubMed
  24. Traish AM, Toselli P, Jeong SJ, Kim NN. Adipocyte accumulation in penile corpus cavernosum of the orchiectomized rabbit: a potential mechanism for veno-occlusive dysfunction in androgen deficiency. J Androl. 2005 Mar-Apr; 26(2):242-8.
    View in: PubMed
  25. Black LD, Brewer KK, Morris SM, Schreiber BM, Toselli P, Nugent MA, Suki B, Stone PJ. Effects of elastase on the mechanical and failure properties of engineered elastin-rich matrices. J Appl Physiol (1985). 2005 Apr; 98(4):1434-41.
    View in: PubMed
  26. Hofmann CS, Wang X, Sullivan CP, Toselli P, Stone PJ, McLean SE, Mecham RP, Schreiber BM, Sonenshein GE. B-Myb represses elastin gene expression in aortic smooth muscle cells. J Biol Chem. 2005 Mar 4; 280(9):7694-701.
    View in: PubMed
  27. Chen LJ, Zhao Y, Gao S, Chou IN, Toselli P, Stone P, Li W. Downregulation of lysyl oxidase and upregulation of cellular thiols in rat fetal lung fibroblasts treated with cigarette smoke condensate. Toxicol Sci. 2005 Feb; 83(2):372-9.
    View in: PubMed
  28. Jones MR, Zhao Z, Sullivan CP, Schreiber BM, Stone PJ, Toselli PA, Kagan HM, Cohen RA, Ravid K. A(3) adenosine receptor deficiency does not influence atherogenesis. J Cell Biochem. 2004 Aug 1; 92(5):1034-43.
    View in: PubMed
  29. Hofmann CS, Sullivan CP, Jiang HY, Stone PJ, Toselli P, Reis ED, Chereshnev I, Schreiber BM, Sonenshein GE. B-Myb represses vascular smooth muscle cell collagen gene expression and inhibits neointima formation after arterial injury. Arterioscler Thromb Vasc Biol. 2004 Sep; 24(9):1608-13.
    View in: PubMed
  30. Zhang Y, Nagata Y, Yu G, Nguyen HG, Jones MR, Toselli P, Jackson CW, Tatsuka M, Todokoro K, Ravid K. Aberrant quantity and localization of Aurora-B/AIM-1 and survivin during megakaryocyte polyploidization and the consequences of Aurora-B/AIM-1-deregulated expression. Blood. 2004 May 15; 103(10):3717-26.
    View in: PubMed
  31. Yaar R, Lamperti ED, Toselli PA, Ravid K. Activity of the A3 adenosine receptor gene promoter in transgenic mice: characterization of previously unidentified sites of expression. FEBS Lett. 2002 Dec 18; 532(3):267-72.
    View in: PubMed
  32. Kaluzhny Y, Yu G, Sun S, Toselli PA, Nieswandt B, Jackson CW, Ravid K. BclxL overexpression in megakaryocytes leads to impaired platelet fragmentation. Blood. 2002 Sep 1; 100(5):1670-8.
    View in: PubMed
  33. Liu B, Lague JR, Nunes DP, Toselli P, Oppenheim FG, Soares RV, Troxler RF, Offner GD. Expression of membrane-associated mucins MUC1 and MUC4 in major human salivary glands. J Histochem Cytochem. 2002 Jun; 50(6):811-20.
    View in: PubMed
  34. Hechler B, Toselli P, Ravanat C, Gachet C, Ravid K. Mpl ligand increases P2Y1 receptor gene expression in megakaryocytes with no concomitant change in platelet response to ADP. Mol Pharmacol. 2001 Nov; 60(5):1112-20.
    View in: PubMed
  35. Sun S, Zimmet JM, Toselli P, Thompson A, Jackson CW, Ravid K. Overexpression of cyclin D1 moderately increases ploidy in megakaryocytes. Haematologica. 2001 Jan; 86(1):17-23.
    View in: PubMed
  36. Trombino AF, Near RI, Matulka RA, Yang S, Hafer LJ, Toselli PA, Kim DW, Rogers AE, Sonenshein GE, Sherr DH. Expression of the aryl hydrocarbon receptor/transcription factor (AhR) and AhR-regulated CYP1 gene transcripts in a rat model of mammary tumorigenesis. Breast Cancer Res Treat. 2000 Sep; 63(2):117-31.
    View in: PubMed
  37. Xu X, Toselli PA, Russell LD, Seldin DC. Globozoospermia in mice lacking the casein kinase II alpha' catalytic subunit. Nat Genet. 1999 Sep; 23(1):118-21.
    View in: PubMed
  38. Hsiao H, Stone PJ, Toselli P, Rosenbloom J, Franzblau C, Schreiber BM. The role of the carboxy terminus of tropoelastin in its assembly into the elastic fiber. Connect Tissue Res. 1999; 40(2):83-95.
    View in: PubMed
  39. Zimmet JM, Toselli P, Ravid K. Cyclin D3 and megakaryocyte development: exploration of a transgenic phenotype. Stem Cells. 1998; 16 Suppl 2:97-106.
    View in: PubMed
  40. Yamaguchi K, Matulka RA, Shneider AM, Toselli P, Trombino AF, Yang S, Hafer LJ, Mann KK, Tao XJ, Tilly JL, Near RI, Sherr DH. Induction of PreB cell apoptosis by 7,12-dimethylbenz[a]anthracene in long-term primary murine bone marrow cultures. Toxicol Appl Pharmacol. 1997 Dec; 147(2):190-203.
    View in: PubMed
  41. Yamaguchi K, Near RI, Matulka RA, Shneider A, Toselli P, Trombino AF, Sherr DH. Activation of the aryl hydrocarbon receptor/transcription factor and bone marrow stromal cell-dependent preB cell apoptosis. J Immunol. 1997 Mar 1; 158(5):2165-73.
    View in: PubMed
  42. Nehra A, Hall SJ, Basile G, Bertero EB, Moreland R, Toselli P, de las Morenas A, Goldstein I. Systemic sclerosis and impotence: a clinicopathological correlation. J Urol. 1995 Apr; 153(4):1140-6.
    View in: PubMed
  43. Traish AM, Gupta S, Toselli P, de Tejada IS, Goldstein I, Moreland RB. Identification of alpha 1-adrenergic receptor subtypes in human corpus cavernosum tissue and in cultured trabecular smooth muscle cells. Receptor. 1995; 5(3):145-57.
    View in: PubMed
  44. Toselli P, Moreland R, Traish AM. Detection of m2 muscarinic acetylcholine receptor mRNA in human corpus cavernosum by in-situ hybridization. Life Sci. 1994; 55(8):621-7.
    View in: PubMed
  45. Aqrabawi J, Schilder H, Toselli P, Franzblau C. Biochemical and histochemical analysis of the enzyme arylsulfatase in human lesions of endodontic origin. J Endod. 1993 Jul; 19(7):335-8.
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  46. Toselli P, Faris B, Sassoon D, Jackson BA, Franzblau C. In-situ hybridization of tropoelastin mRNA during the development of the multilayered neonatal rat aortic smooth muscle cell culture. Matrix. 1992 Aug; 12(4):321-32.
    View in: PubMed
  47. Schreiber BM, Jones HV, Toselli P, Franzblau C. Long-term treatment of neonatal aortic smooth muscle cells with beta VLDL induces cholesterol accumulation. Atherosclerosis. 1992 Aug; 95(2-3):201-10.
    View in: PubMed
  48. Faris B, Tan OT, Toselli P, Franzblau C. Long-term neonatal rat aortic smooth muscle cell cultures: a model for the tunica media of a blood vessel. Matrix. 1992 Jun; 12(3):185-8.
    View in: PubMed
  49. Martin BM, Ritchie AR, Toselli P, Franzblau C. Elastin synthesis and accumulation in irradiated smooth muscle cell cultures. Connect Tissue Res. 1992; 28(3):181-9.
    View in: PubMed
  50. Faris B, Toselli P, Kispert J, Wolfe BL, Pratt CA, Mogayzel PJ, Franzblau C. Elastase effect on the extracellular matrix of rat aortic smooth muscle cells in culture. Exp Mol Pathol. 1986 Oct; 45(2):105-17.
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  51. Pistone AM, Podestà F, Raviolo E, Testa D, Grosso B, Toselli P, Nyffeneger G. [Cholestatic fatty liver of probably iatrogenic origin]. Recenti Prog Med. 1986 Apr; 77(4):188-90.
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  52. McMahon MP, Faris B, Wolfe BL, Brown KE, Pratt CA, Toselli P, Franzblau C. Aging effects on the elastin composition in the extracellular matrix of cultured rat aortic smooth muscle cells. In Vitro Cell Dev Biol. 1985 Dec; 21(12):674-80.
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  53. Barone LM, Faris B, Chipman SD, Toselli P, Oakes BW, Franzblau C. Alteration of the extracellular matrix of smooth muscle cells by ascorbate treatment. Biochim Biophys Acta. 1985 Jun 18; 840(2):245-54.
    View in: PubMed
  54. Resegotti L, Pistone MA, Testa D, Charbonnier R, Toselli P, Vivalda S. [Bone marrow culture in patients treated with Ticlopidine]. Nouv Rev Fr Hematol. 1985; 27(1):19-22.
    View in: PubMed
  55. Hu CL, Toselli P, Franzblau C. Synthesis and turnover of protein-bound Nt-methylhistidine in cultured vascular smooth muscle cells. Surv Synth Pathol Res. 1985; 4(5-6):380-8.
    View in: PubMed
  56. Toselli P, Faris B, Oliver P, Franzblau C. Ultrastructural studies of attachment site formation in aortic smooth muscle cells cultured on collagen-hydroxyethylmethacrylate hydrogels. J Ultrastruct Res. 1984 Mar; 86(3):252-61.
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  57. Faris B, Ferrera R, Toselli P, Nambu J, Gonnerman WA, Franzblau C. Effect of varying amounts of ascorbate on collagen, elastin and lysyl oxidase synthesis in aortic smooth muscle cell cultures. Biochim Biophys Acta. 1984 Jan 24; 797(1):71-5.
    View in: PubMed
  58. Toselli P, Mogayzel PJ, Faris B, Ferrera R, Franzblau C. Mammalian cell growth on collagen-hydrogels. Scan Electron Microsc. 1984; (Pt 3):1301-12.
    View in: PubMed
  59. Wedel N, Toselli P, Pothoulakis C, Faris B, Oliver P, Franzblau C, LaMont T. Ultrastructural effects of Clostridium difficile toxin B on smooth muscle cells and fibroblasts. Exp Cell Res. 1983 Oct 15; 148(2):413-22.
    View in: PubMed
  60. Toselli P, Faris B, Oliver P, Wedel N, Franzblau C. Preservation of ultrastructure of cells cultured on protein-hydroxyethylmethacrylate hydrogels. J Ultrastruct Res. 1983 May; 83(2):220-31.
    View in: PubMed
  61. Faris B, Mozzicato P, Ferrera R, Glembourtt M, Toselli P, Franzblau C. Collagen of brain microvessel preparations. Microvasc Res. 1982 Mar; 23(2):171-9.
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  62. Szycher M, Poirier V, Franzblau C, Faris B, Snider R, Toselli P, Haudenschild CC, Bernhard WF. Biochemical, histological, and ultrastructural assessments of pseudoneointimal linings derived from fibroblast-seeded integrally textured polymeric surfaces. J Biomed Mater Res. 1981 Mar; 15(2):247-65.
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  63. Toselli P, Salcedo LL, Oliver P, Franzblau C. Formation of elastic fibers and elastin in rabbit aortic smooth muscle cell cultures. Connect Tissue Res. 1981; 8(3-4):231-9.
    View in: PubMed
  64. Toselli P, Oliver P, Cox M, Faris B, Franzblau C. Ultrastructural studies of a polygonal myofilament network in cultured rat aortic smooth muscle cells. Cell Motil. 1981; 1(2):193-203.
    View in: PubMed
  65. Molaschi M, Toselli P, Poli L, Pecchio F, Fabris F. [Determination of immunoglobulins in the aged. Normal range and changes in pathological conditions]. Minerva Med. 1980 Jan 14; 71(1):25-32.
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  66. Szycher M, Poirier V, Bernhard WF, Franzblau C, Haudenschild CC, Toselli P. Integrally textured polymeric surfaces for permanently implantable cardiac assist devices. Trans Am Soc Artif Intern Organs. 1980; 26:493-7.
    View in: PubMed
  67. Blitz AL, Fine RE, Toselli PA. Evidence that coated vesicles isolated from brain are calcium-sequestering organelles resembling sarcoplasmic reticulum. J Cell Biol. 1977 Oct; 75(1):135-47.
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  68. Ullrick WC, Toselli PA, Saide JD, Phear WP. Fine structure of the vertebrate Z-disc. J Mol Biol. 1977 Sep; 115(1):61-74.
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  69. Ullrick WC, Toselli PA, Chase D, Dasse K. Are there extensions of thick filaments to the Z line in vertebrate and invertebrate striated muscle? J Ultrastruct Res. 1977 Aug; 60(2):263-71.
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  70. Toselli PA, Harbison GR. The fine structure of developing locomotor muscles of the pelagic tunicate, cyclosalpa affinis (Thaliacea: Salpidae). Tissue Cell. 1977; 9(1):137-56.
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  71. Berk SL, Block PJ, Toselli PA, Ullrick WC. The effects of chronic alcohol ingestion in mice on contractile properties of cardiac and skeletal muscle: a comparison with normal and dehydrated-malnourished controls. Experientia. 1975 Nov 15; 31(11):1302-3.
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  72. Roesing TG, Toselli PA, Crowell RL. Elution and uncoating of Coxsackievirus B3 by isolated HeLa cell plasma membranes. J Virol. 1975 Mar; 15(3):654-7.
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  73. Toselli PA, Pepe FA. The fine structure of the ventral intersegmental abdominal muscles of the insect Rhodnius prolixus during the molting cycle. II. Muscle changes in preparation for molting. J Cell Biol. 1968 May; 37(2):462-81.
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  74. Toselli PA, Pepe FA. The fine structure of the ventral intersegmental abdominal muscles of the insect Rhodnius prolixus during the molting cycle. I. Muscle structure at molting. J Cell Biol. 1968 May; 37(2):445-61.
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