Keywords
Last Name

Suryaram Gummuluru, PhD

TitleAssociate Professor
InstitutionBoston University School of Medicine
DepartmentMicrobiology
Address72 E. Concord St Housman (R)
Boston MA 02118
Phone(617) 414-8075
ORCID ORCID Icon0000-0002-8606-8481
Other Positions
TitleGraduate Faculty (Primary Mentor of Grad Students)
InstitutionBoston University School of Medicine, Graduate Medical Sciences

 Research Expertise & Professional Interests
Our research is broadly focused on the role of dendritic cells (DCs) in the initiation and propagation of HIV-1 replication. Since dendritic cells are believed to be the first immune competent cells to encounter virus in the genital mucosa, a thorough understanding of the HIV-DC interactions is of paramount importance. DCs can capture virus particles independently of CD4 and co-receptor complexes, and retain them in an infectious state for an extended period of time. These virus-bearing DCs may then facilitate a more efficient spread of virus to replication-permissive CD4+ T cells. Our recent work has identified a novel glycosphingolipid dependent mechanism of virus attachment to DCs. The fate of the virus particle post-attachment in DCs remains unclear. Virion trafficking within the DC bypasses conventional endocytic organelles, i.e., endosomes and lysosomes. Virus localization within this novel vesicular compartment not only has the potential to protect the invading HIV from being degraded, but also creates a latent reservoir of virus, which could present a major challenge for eradication by antiretroviral therapy. Furthermore, the mechanism of subsequent return of infectious virus particles to the cell surface and the method of subsequent transmission to T cells remains unclear. Current studies utilizing biochemical and microscopic approaches are underway to monitor HIV-1 trafficking and localization in the DC and its subsequent transfer to T cells.

 Self-Described Keywords
  • dendritic cells
  • glycosphingolipids
  • HIV pathogenesis
  • Virological synapse
  • virus attachment, trafficking, and cell-mediated virus transfer
 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Colvin TA, Gabai VL, Gong J, Calderwood SK, Li H, Gummuluru S, Matchuk ON, Smirnova SG, Orlova NV, Zamulaeva IA, Garcia-Marcos M, Li X, Young ZT, Rauch JN, Gestwicki JE, Takayama S, Sherman MY. Hsp70-bag3 interactions regulate cancer-related signaling networks. Cancer Res. 2014 Sep 1; 74(17):4731-40.
    View in: PubMed
  2. Yu X, Feizpour A, Ramirez NG, Wu L, Akiyama H, Xu F, Gummuluru S, Reinhard BM. Glycosphingolipid-functionalized nanoparticles recapitulate CD169-dependent HIV-1 uptake and trafficking in dendritic cells. Nat Commun. 2014; 5:4136.
    View in: PubMed
  3. Akiyama H, Miller C, Patel HV, Hatch SC, Archer J, Ramirez NG, Gummuluru S. Virus Particle Release from Glycosphingolipid-Enriched Microdomains Is Essential for Dendritic Cell-Mediated Capture and Transfer of HIV-1 and Henipavirus. J Virol. 2014 Aug 15; 88(16):8813-25.
    View in: PubMed
  4. Puryear WB, Akiyama H, Geer SD, Ramirez NP, Yu X, Reinhard BM, Gummuluru S. Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169. PLoS Pathog. 2013; 9(4):e1003291.
    View in: PubMed
  5. Puryear WB, Gummuluru S. Role of glycosphingolipids in dendritic cell-mediated HIV-1 trans-infection. Adv Exp Med Biol. 2013; 762:131-53.
    View in: PubMed
  6. Schiralli Lester GM, Akiyama H, Evans E, Singh J, Gummuluru S, Henderson AJ. Interleukin 2-inducible T cell kinase (ITK) facilitates efficient egress of HIV-1 by coordinating Gag distribution and actin organization. Virology. 2013 Feb 5; 436(1):235-43.
    View in: PubMed
  7. Waheed AA, Brass AL, Gummuluru S, Tachedjian G. Host-pathogen interactions of retroviruses. Mol Biol Int. 2012; 2012:648512.
    View in: PubMed
  8. Puryear WB, Yu X, Ramirez NP, Reinhard BM, Gummuluru S. HIV-1 incorporation of host-cell-derived glycosphingolipid GM3 allows for capture by mature dendritic cells. Proc Natl Acad Sci U S A. 2012 May 8; 109(19):7475-80.
    View in: PubMed
  9. Sagar M, Akiyama H, Etemad B, Ramirez N, Freitas I, Gummuluru S. Transmembrane domain membrane proximal external region but not surface unit-directed broadly neutralizing HIV-1 antibodies can restrict dendritic cell-mediated HIV-1 trans-infection. J Infect Dis. 2012 Apr 15; 205(8):1248-57.
    View in: PubMed
  10. Hanley TM, Blay Puryear W, Gummuluru S, Viglianti GA. PPARgamma and LXR signaling inhibit dendritic cell-mediated HIV-1 capture and trans-infection. PLoS Pathog. 2010; 6:e1000981.
    View in: PubMed
  11. Hatch SC, Archer J, Gummuluru S. Glycosphingolipid composition of human immunodeficiency virus type 1 (HIV-1) particles is a crucial determinant for dendritic cell-mediated HIV-1 trans-infection. J Virol. 2009 Apr; 83(8):3496-506.
    View in: PubMed
  12. Izquierdo-Useros N, Naranjo-Gómez M, Archer J, Hatch SC, Erkizia I, Blanco J, Borràs FE, Puertas MC, Connor JH, Fernández-Figueras MT, Moore L, Clotet B, Gummuluru S, Martinez-Picado J. Capture and transfer of HIV-1 particles by mature dendritic cells converges with the exosome-dissemination pathway. Blood. 2009 Mar 19; 113(12):2732-41.
    View in: PubMed
  13. Nguyen DH, Gummuluru S, Hu J. Deamination-independent inhibition of hepatitis B virus reverse transcription by APOBEC3G. J Virol. 2007 May; 81(9):4465-72.
    View in: PubMed
  14. Wiley RD, Gummuluru S. Immature dendritic cell-derived exosomes can mediate HIV-1 trans infection. Proc Natl Acad Sci U S A. 2006 Jan 17; 103(3):738-43.
    View in: PubMed
  15. Gummuluru S, Rogel M, Stamatatos L, Emerman M. Binding of human immunodeficiency virus type 1 to immature dendritic cells can occur independently of DC-SIGN and mannose binding C-type lectin receptors via a cholesterol-dependent pathway. J Virol. 2003 Dec; 77(23):12865-74.
    View in: PubMed
  16. Gummuluru S, KewalRamani VN, Emerman M. Dendritic cell-mediated viral transfer to T cells is required for human immunodeficiency virus type 1 persistence in the face of rapid cell turnover. J Virol. 2002 Nov; 76(21):10692-701.
    View in: PubMed
  17. Gummuluru S, Emerman M. Advances in HIV molecular biology. AIDS. 2002; 16 Suppl 4:S17-23.
    View in: PubMed
  18. Gummuluru S, Kinsey CM, Emerman M. An in vitro rapid-turnover assay for human immunodeficiency virus type 1 replication selects for cell-to-cell spread of virus. J Virol. 2000 Dec; 74(23):10882-91.
    View in: PubMed
  19. Gummuluru S, Emerman M. Cell cycle- and Vpr-mediated regulation of human immunodeficiency virus type 1 expression in primary and transformed T-cell lines. J Virol. 1999 Jul; 73(7):5422-30.
    View in: PubMed
  20. Whetter L, Novembre FJ, Saucier M, Gummuluru S, Dewhurst S. Costimulatory pathways in lymphocyte proliferation induced by the simian immunodeficiency virus SIVsmmPBj14. J Virol. 1998 Jul; 72(7):6155-8.
    View in: PubMed
  21. Krajewski S, James HJ, Ross J, Blumberg BM, Epstein LG, Gendelman HE, Gummuluru S, Dewhurst S, Sharer LR, Reed JC, Gelbard HA. Expression of pro- and anti-apoptosis gene products in brains from paediatric patients with HIV-1 encephalitis. Neuropathol Appl Neurobiol. 1997 Jun; 23(3):242-53.
    View in: PubMed
  22. Gummuluru S, Novembre FJ, Lewis M, Gelbard HA, Dewhurst S. Apoptosis correlates with immune activation in intestinal lymphoid tissue from macaques acutely infected by a highly enteropathic simian immunodeficiency virus, SIVsmmPBj14. Virology. 1996 Nov 1; 225(1):21-32.
    View in: PubMed
  23. Gummuluru S, Novembre FJ, Seshi B, Dewhurst S. SIVsmmPBj14 induces expression of a mucosal integrin on macaque lymphocytes. Virology. 1996 Jan 1; 215(1):97-100.
    View in: PubMed
  24. van Loon NM, Gummuluru S, Sherwood DJ, Marentes R, Hall CB, Dewhurst S. Direct sequence analysis of human herpesvirus 6 (HHV-6) sequences from infants and comparison of HHV-6 sequences from mother/infant pairs. Clin Infect Dis. 1995 Oct; 21(4):1017-9.
    View in: PubMed
  25. Talley AK, Dewhurst S, Perry SW, Dollard SC, Gummuluru S, Fine SM, New D, Epstein LG, Gendelman HE, Gelbard HA. Tumor necrosis factor alpha-induced apoptosis in human neuronal cells: protection by the antioxidant N-acetylcysteine and the genes bcl-2 and crmA. Mol Cell Biol. 1995 May; 15(5):2359-66.
    View in: PubMed
  26. Dollard SC, Gummuluru S, Tsang S, Fultz PN, Dewhurst S. Enhanced responsiveness to nuclear factor kappa B contributes to the unique phenotype of simian immunodeficiency virus variant SIVsmmPBj14. J Virol. 1994 Dec; 68(12):7800-9.
    View in: PubMed
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