Keywords
Last Name

Phillips Wesley Robbins, PhD

TitleProfessor
InstitutionBoston University Goldman School of Dental Medicine
DepartmentMolecular & Cell Biology
Address75 E. Newton St
Boston MA 02118
Phone(617) 414-1047
ORCID ORCID Icon0000-0002-5121-1524
Other Positions
TitleGraduate Faculty (Primary Mentor of Grad Students)
InstitutionBoston University School of Medicine
DepartmentGraduate Medical Sciences, Division of

 Research Expertise & Professional Interests
Expertise in Chitin Synthesis:

In Saccharomyces cerevisiae most chitin is synthesized by Chs3p, which deposits chitin in the lateral cell wall and in the bud-neck region during cell division. We have recently found that addition of glucosamine (GlcN) to the growth medium leads to a three- to fourfold increase in cell wall chitin levels. We compared this result to the increases in cellular chitin levels associated with cell wall stress and with treatment of yeast with mating pheromone. Since all three phenomena lead to increases in precursors of chitin, we hypothesized that chitin synthesis is at least in part directly regulated by the size of this pool. This hypothesis was strengthened by our finding that addition of GlcN to the growth medium causes a rapid increase in chitin synthesis without any pronounced change in the expression of more than 6,000 genes monitored with Affymetrix gene expression chips. In other studies we found that the specific activity of Chs3p is higher in the total membrane fractions from cells grown in GlcN and from mutants with weakened cell walls. Sucrose gradient analysis shows that Chs3p is present in an inactive form in what may be Golgi compartments but as an active enzyme in other intracellular membrane-bound vesicles, as well as in the plasma membrane. We conclude that Chs3p-dependent chitin synthesis in S. cerevisiae is regulated both by the levels of intermediates of the UDP-GlcNAc biosynthetic pathway and by an increase in the activity of the enzyme in the plasma membrane.

Expertise in N-Linked Glycosylation in Protists (collaboration with John Samuelson):

The vast majority of eukaryotes (fungi, plants, animals, slime mold, and euglena) synthesize Asn-linked glycans (Alg) by means of a lipid-linked precursor dolichol-PP-GlcNAc2Man9Glc3. Knowledge of this pathway is important because defects in the glycosyltransferases (Alg1-Alg12 and others not yet identified), which make dolichol-PP-glycans, lead to numerous congenital disorders of glycosylation. We used bioinformatics and experimental methods to characterize Alg glycosyltransferases and dolichol-PP-glycans of diverse protists, including many human pathogens, with the following major conclusions. First, it is demonstrated that common ancestry is a useful method of predicting the Alg glycosyltransferase inventory of each eukaryote. Second, in the vast majority of cases, this inventory accurately predicts the dolichol-PP-glycans observed. Third, Alg glycosyltransferases are missing in sets from each organism (e.g., all of the glycosyltransferases that add glucose and mannose are absent from Giardia and Plasmodium). Fourth, dolichol-PP-GlcNAc2Man5 (present in Entamoeba and Trichomonas) and dolichol-PP- and N-linked GlcNAc2 (present in Giardia) have not been identified previously in wild-type organisms. Finally, the present diversity of protist and fungal dolichol-PP-linked glycans appears to result from secondary loss of glycosyltransferases from a common ancestor that contained the complete set of Alg glycosyltransferases.

 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Samuelson J, Bushkin GG, Chatterjee A, Robbins PW. Strategies to discover the structural components of cyst and oocyst walls. Eukaryot Cell. 2013 Dec; 12(12):1578-87.
    View in: PubMed
  2. Bushkin GG, Motari E, Carpentieri A, Dubey JP, Costello CE, Robbins PW, Samuelson J. Evidence for a structural role for acid-fast lipids in oocyst walls of Cryptosporidium, Toxoplasma, and Eimeria. MBio. 2013; 4(5):e00387-13.
    View in: PubMed
  3. Bushkin GG, Motari E, Magnelli P, Gubbels MJ, Dubey JP, Miska KB, Bullitt E, Costello CE, Robbins PW, Samuelson J. ß-1,3-glucan, which can be targeted by drugs, forms a trabecular scaffold in the oocyst walls of Toxoplasma and Eimeria. MBio. 2012; 3(5).
    View in: PubMed
  4. Samuelson J, Robbins P. A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia. Trends Parasitol. 2011 Jan; 27(1):17-22.
    View in: PubMed
  5. Robbins P. Stents, statins, and other dumb luck. J Biol Chem. 2011 Jan 21; 286(3):1669-74.
    View in: PubMed
  6. Carpentieri A, Ratner DM, Ghosh SK, Banerjee S, Bushkin GG, Cui J, Lubrano M, Steffen M, Costello CE, O'Keefe B, Robbins PW, Samuelson J. The antiretroviral lectin cyanovirin-N targets well-known and novel targets on the surface of Entamoeba histolytica trophozoites. Eukaryot Cell. 2010 Nov; 9(11):1661-8.
    View in: PubMed
  7. Chatterjee A, Carpentieri A, Ratner DM, Bullitt E, Costello CE, Robbins PW, Samuelson J. Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer. PLoS Pathog. 2010; 6(8):e1001059.
    View in: PubMed
  8. Ghosh SK, Van Dellen KL, Chatterjee A, Dey T, Haque R, Robbins PW, Samuelson J. The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic. PLoS Negl Trop Dis. 2010; 4(7):e750.
    View in: PubMed
  9. Mitra S, Cui J, Robbins PW, Samuelson J. A deeply divergent phosphoglucomutase (PGM) of Giardia lamblia has both PGM and phosphomannomutase activities. Glycobiology. 2010 Oct; 20(10):1233-40.
    View in: PubMed
  10. Grabinska KA, Cui J, Chatterjee A, Guan Z, Raetz CR, Robbins PW, Samuelson J. Molecular characterization of the cis-prenyltransferase of Giardia lamblia. Glycobiology. 2010 Jul; 20(7):824-32.
    View in: PubMed
  11. Chatterjee A, Banerjee S, Steffen M, O'Connor RM, Ward HD, Robbins PW, Samuelson J. Evidence for mucin-like glycoproteins that tether sporozoites of Cryptosporidium parvum to the inner surface of the oocyst wall. Eukaryot Cell. 2010 Jan; 9(1):84-96.
    View in: PubMed
  12. Bushkin GG, Ratner DM, Cui J, Banerjee S, Duraisingh MT, Jennings CV, Dvorin JD, Gubbels MJ, Robertson SD, Steffen M, O'Keefe BR, Robbins PW, Samuelson J. Suggestive evidence for Darwinian Selection against asparagine-linked glycans of Plasmodium falciparum and Toxoplasma gondii. Eukaryot Cell. 2010 Feb; 9(2):228-41.
    View in: PubMed
  13. Cui J, Smith T, Robbins PW, Samuelson J. Darwinian selection for sites of Asn-linked glycosylation in phylogenetically disparate eukaryotes and viruses. Proc Natl Acad Sci U S A. 2009 Aug 11; 106(32):13421-6.
    View in: PubMed
  14. Chatterjee A, Ghosh SK, Jang K, Bullitt E, Moore L, Robbins PW, Samuelson J. Evidence for a "wattle and daub" model of the cyst wall of entamoeba. PLoS Pathog. 2009 Jul; 5(7):e1000498.
    View in: PubMed
  15. Banerjee S, Robbins PW, Samuelson J. Molecular characterization of nucleocytosolic O-GlcNAc transferases of Giardia lamblia and Cryptosporidium parvum. Glycobiology. 2009 Apr; 19(4):331-6.
    View in: PubMed
  16. Ratner DM, Cui J, Steffen M, Moore LL, Robbins PW, Samuelson J. Changes in the N-glycome, glycoproteins with Asn-linked glycans, of Giardia lamblia with differentiation from trophozoites to cysts. Eukaryot Cell. 2008 Nov; 7(11):1930-40.
    View in: PubMed
  17. Grabinska KA, Ghosh SK, Guan Z, Cui J, Raetz CR, Robbins PW, Samuelson J. Dolichyl-phosphate-glucose is used to make O-glycans on glycoproteins of Trichomonas vaginalis. Eukaryot Cell. 2008 Aug; 7(8):1344-51.
    View in: PubMed
  18. Magnelli P, Cipollo JF, Ratner DM, Cui J, Kelleher D, Gilmore R, Costello CE, Robbins PW, Samuelson J. Unique Asn-linked oligosaccharides of the human pathogen Entamoeba histolytica. J Biol Chem. 2008 Jun 27; 283(26):18355-64.
    View in: PubMed
  19. Banerjee S, Cui J, Robbins PW, Samuelson J. Use of Giardia, which appears to have a single nucleotide-sugar transporter for UDP-GlcNAc, to identify the UDP-Glc transporter of Entamoeba. Mol Biochem Parasitol. 2008 May; 159(1):44-53.
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  20. Banerjee S, Vishwanath P, Cui J, Kelleher DJ, Gilmore R, Robbins PW, Samuelson J. The evolution of N-glycan-dependent endoplasmic reticulum quality control factors for glycoprotein folding and degradation. Proc Natl Acad Sci U S A. 2007 Jul 10; 104(28):11676-81.
    View in: PubMed
  21. Grabinska KA, Magnelli P, Robbins PW. Prenylation of Saccharomyces cerevisiae Chs4p Affects Chitin Synthase III activity and chitin chain length. Eukaryot Cell. 2007 Feb; 6(2):328-36.
    View in: PubMed
  22. Prakash S, Paul WE, Robbins PW. Fibrosin, a novel fibrogenic cytokine, modulates expression of myofibroblasts. Exp Mol Pathol. 2007 Feb; 82(1):42-8.
    View in: PubMed
  23. Van Dellen KL, Chatterjee A, Ratner DM, Magnelli PE, Cipollo JF, Steffen M, Robbins PW, Samuelson J. Unique posttranslational modifications of chitin-binding lectins of Entamoeba invadens cyst walls. Eukaryot Cell. 2006 May; 5(5):836-48.
    View in: PubMed
  24. Das S, Van Dellen K, Bulik D, Magnelli P, Cui J, Head J, Robbins PW, Samuelson J. The cyst wall of Entamoeba invadens contains chitosan (deacetylated chitin). Mol Biochem Parasitol. 2006 Jul; 148(1):86-92.
    View in: PubMed
  25. Van Dellen KL, Bulik DA, Specht CA, Robbins PW, Samuelson JC. Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae. Eukaryot Cell. 2006 Jan; 5(1):203-6.
    View in: PubMed
  26. Robbins PW, Samuelson J. Asparagine linked glycosylation in Giardia. Glycobiology. 2005 Jun; 15(6):15G-16G.
    View in: PubMed
  27. Samuelson J, Banerjee S, Magnelli P, Cui J, Kelleher DJ, Gilmore R, Robbins PW. The diversity of dolichol-linked precursors to Asn-linked glycans likely results from secondary loss of sets of glycosyltransferases. Proc Natl Acad Sci U S A. 2005 Feb 1; 102(5):1548-53.
    View in: PubMed
  28. Magnelli PE, Cipollo JF, Robbins PW. A glucanase-driven fractionation allows redefinition of Schizosaccharomyces pombe cell wall composition and structure: assignment of diglucan. Anal Biochem. 2005 Jan 15; 336(2):202-12.
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  29. Lucero HA, Robbins PW. Lipid rafts-protein association and the regulation of protein activity. Arch Biochem Biophys. 2004 Jun 15; 426(2):208-24.
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  30. Cipollo JF, Awad A, Costello CE, Robbins PW, Hirschberg CB. Biosynthesis in vitro of Caenorhabditis elegans phosphorylcholine oligosaccharides. Proc Natl Acad Sci U S A. 2004 Mar 9; 101(10):3404-8.
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  31. Bulik DA, Olczak M, Lucero HA, Osmond BC, Robbins PW, Specht CA. Chitin synthesis in Saccharomyces cerevisiae in response to supplementation of growth medium with glucosamine and cell wall stress. Eukaryot Cell. 2003 Oct; 2(5):886-900.
    View in: PubMed
  32. Bulik DA, Robbins PW. The Caenorhabditis elegans sqv genes and functions of proteoglycans in development. Biochim Biophys Acta. 2002 Dec 19; 1573(3):247-57.
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  33. Van Dellen K, Ghosh SK, Robbins PW, Loftus B, Samuelson J. Entamoeba histolytica lectins contain unique 6-Cys or 8-Cys chitin-binding domains. Infect Immun. 2002 Jun; 70(6):3259-63.
    View in: PubMed
  34. Bulik DA, Wei G, Toyoda H, Kinoshita-Toyoda A, Waldrip WR, Esko JD, Robbins PW, Selleck SB. sqv-3, -7, and -8, a set of genes affecting morphogenesis in Caenorhabditis elegans, encode enzymes required for glycosaminoglycan biosynthesis. Proc Natl Acad Sci U S A. 2000 Sep 26; 97(20):10838-43.
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  35. Prakash S, Robbins PW. Glycotyping of prostate specific antigen. Glycobiology. 2000 Feb; 10(2):173-6.
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  36. Southard SB, Specht CA, Mishra C, Chen-Weiner J, Robbins PW. Molecular analysis of the Candida albicans homolog of Saccharomyces cerevisiae MNN9, required for glycosylation of cell wall mannoproteins. J Bacteriol. 1999 Dec; 181(24):7439-48.
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  37. Osmond BC, Specht CA, Robbins PW. Chitin synthase III: synthetic lethal mutants and "stress related" chitin synthesis that bypasses the CSD3/CHS6 localization pathway. Proc Natl Acad Sci U S A. 1999 Sep 28; 96(20):11206-10.
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  38. Robbins PW. Yeast glycosylation - foundations and building blocks. Biochim Biophys Acta. 1999 Jan 6; 1426(2):225-6.
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  39. Rodríguez-Medina JR, Cruz JA, Robbins PW, Bi E, Pringle JR. Elevated expression of chitinase 1 and chitin synthesis in myosin II-deficient Saccharomyces cerevisiae. Cell Mol Biol (Noisy-le-grand). 1998 Sep; 44(6):919-25.
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  40. de la Vega H, Specht CA, Liu Y, Robbins PW. Chitinases are a multi-gene family in Aedes, Anopheles and Drosophila. Insect Mol Biol. 1998 Aug; 7(3):233-9.
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  41. Mark BL, Wasney GA, Salo TJ, Khan AR, Cao Z, Robbins PW, James MN, Triggs-Raine BL. Structural and functional characterization of Streptomyces plicatus beta-N-acetylhexosaminidase by comparative molecular modeling and site-directed mutagenesis. J Biol Chem. 1998 Jul 31; 273(31):19618-24.
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  42. Wysong DR, Christin L, Sugar AM, Robbins PW, Diamond RD. Cloning and sequencing of a Candida albicans catalase gene and effects of disruption of this gene. Infect Immun. 1998 May; 66(5):1953-61.
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  43. Hirschberg CB, Robbins PW, Abeijon C. Transporters of nucleotide sugars, ATP, and nucleotide sulfate in the endoplasmic reticulum and Golgi apparatus. Annu Rev Biochem. 1998; 67:49-69.
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  44. Xoconostle-Cázares B, Specht CA, Robbins PW, Liu Y, León C, Ruiz-Herrera J. Umchs5, a gene coding for a class IV chitin synthase in Ustilago maydis. Fungal Genet Biol. 1997 Dec; 22(3):199-208.
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  45. Lussier M, White AM, Sheraton J, di Paolo T, Treadwell J, Southard SB, Horenstein CI, Chen-Weiner J, Ram AF, Kapteyn JC, Roemer TW, Vo DH, Bondoc DC, Hall J, Zhong WW, Sdicu AM, Davies J, Klis FM, Robbins PW, Bussey H. Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics. 1997 Oct; 147(2):435-50.
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  46. Bakkers J, Semino CE, Stroband H, Kijne JW, Robbins PW, Spaink HP. An important developmental role for oligosaccharides during early embryogenesis of cyprinid fish. Proc Natl Acad Sci U S A. 1997 Jul 22; 94(15):7982-6.
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  47. de la Vega H, Specht CA, Semino CE, Robbins PW, Eichinger D, Caplivski D, Ghosh S, Samuelson J. Cloning and expression of chitinases of Entamoebae. Mol Biochem Parasitol. 1997 Apr; 85(2):139-47.
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  48. Mishra C, Semino CE, McCreath KJ, de la Vega H, Jones BJ, Specht CA, Robbins PW. Cloning and expression of two chitin deacetylase genes of Saccharomyces cerevisiae. Yeast. 1997 Mar 30; 13(4):327-36.
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  49. de la Vega H, Specht CA, Chau A, Semino CE, Robbins PW, Eichinger D, Caplivski D, Ghosh S, Samuelson J. Cyst-specific exochitinases of Entamoebae contain unique hydrophilic repeats at their amino termini. Arch Med Res. 1997; 28 Spec No:143-6.
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  50. Samuelson J, Caplivski D, Sturm-Ramirez K, Kretzinger K, Descoteaux S, Hernández G, Vargas G, Mammo R, Newton O, Santos J, de la Vega H, Robbins P, Ganguly C, Lohia A. A proposal for a molecular biologic system for classifying isolates of Entamoeba histolytica and Entamoeba dispar. Arch Med Res. 1997; 28 Spec No:274-5.
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  51. Southworth MW, Fuhrman JA, Robbins PW, Beauregard K, Perler FB. Gene cloning and production of active recombinant Brugia malayi microfilarial chitinase. Gene. 1996 Oct 24; 177(1-2):55-8.
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  52. Mellado E, Specht CA, Robbins PW, Holden DW. Cloning and characterization of chsD, a chitin synthase-like gene of Aspergillus fumigatus. FEMS Microbiol Lett. 1996 Sep 15; 143(1):69-76.
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  53. Reiss G, te Heesen S, Zimmerman J, Robbins PW, Aebi M. Isolation of the ALG6 locus of Saccharomyces cerevisiae required for glucosylation in the N-linked glycosylation pathway. Glycobiology. 1996 Jul; 6(5):493-8.
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  54. Zimmerman JW, Specht CA, Cazares BX, Robbins PW. The isolation of a Dol-P-Man synthase from Ustilago maydis that functions in Saccharomyces cerevisiae. Yeast. 1996 Jun 30; 12(8):765-71.
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  55. Abeijon C, Robbins PW, Hirschberg CB. Molecular cloning of the Golgi apparatus uridine diphosphate-N-acetylglucosamine transporter from Kluyveromyces lactis. Proc Natl Acad Sci U S A. 1996 Jun 11; 93(12):5963-8.
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  56. Specht CA, Liu Y, Robbins PW, Bulawa CE, Iartchouk N, Winter KR, Riggle PJ, Rhodes JC, Dodge CL, Culp DW, Borgia PT. The chsD and chsE genes of Aspergillus nidulans and their roles in chitin synthesis. Fungal Genet Biol. 1996 Jun; 20(2):153-67.
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  57. Semino CE, Specht CA, Raimondi A, Robbins PW. Homologs of the Xenopus developmental gene DG42 are present in zebrafish and mouse and are involved in the synthesis of Nod-like chitin oligosaccharides during early embryogenesis. Proc Natl Acad Sci U S A. 1996 May 14; 93(10):4548-53.
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  58. Abeijon C, Mandon EC, Robbins PW, Hirschberg CB. A mutant yeast deficient in Golgi transport of uridine diphosphate N-acetylglucosamine. J Biol Chem. 1996 Apr 12; 271(15):8851-4.
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  59. McCreath KJ, Specht CA, Liu Y, Robbins PW. Molecular cloning of a third chitinase gene (CHT1) from Candida albicans. Yeast. 1996 Apr; 12(5):501-4.
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  60. Din AB, Specht CA, Robbins PW, Yarden O. chs-4, a class IV chitin synthase gene from Neurospora crassa. Mol Gen Genet. 1996 Feb 5; 250(2):214-22.
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  61. Mishra C, Robbins PW. Specific beta glucanases as tools for polysaccharide structure determination. Glycobiology. 1995 Oct; 5(7):645-54.
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  62. Semino CE, Robbins PW. Synthesis of "Nod"-like chitin oligosaccharides by the Xenopus developmental protein DG42. Proc Natl Acad Sci U S A. 1995 Apr 11; 92(8):3498-501.
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  63. McCreath KJ, Specht CA, Robbins PW. Molecular cloning and characterization of chitinase genes from Candida albicans. Proc Natl Acad Sci U S A. 1995 Mar 28; 92(7):2544-8.
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  64. Prakash S, Robbins PW, Wyler DJ. Cloning and analysis of murine cDNA that encodes a fibrogenic lymphokine, fibrosin. Proc Natl Acad Sci U S A. 1995 Mar 14; 92(6):2154-8.
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  65. Mellado E, Aufauvre-Brown A, Specht CA, Robbins PW, Holden DW. A multigene family related to chitin synthase genes of yeast in the opportunistic pathogen Aspergillus fumigatus. Mol Gen Genet. 1995 Feb 6; 246(3):353-9.
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  66. Kollár R, Petráková E, Ashwell G, Robbins PW, Cabib E. Architecture of the yeast cell wall. The linkage between chitin and beta(1-->3)-glucan. J Biol Chem. 1995 Jan 20; 270(3):1170-8.
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  67. Gow NA, Robbins PW, Lester JW, Brown AJ, Fonzi WA, Chapman T, Kinsman OS. A hyphal-specific chitin synthase gene (CHS2) is not essential for growth, dimorphism, or virulence of Candida albicans. Proc Natl Acad Sci U S A. 1994 Jun 21; 91(13):6216-20.
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  68. Zimmerman JW, Robbins PW. The hydrophobic domain of dolichyl-phosphate-mannose synthase is not essential for enzyme activity or growth in Saccharomyces cerevisiae. J Biol Chem. 1993 Aug 5; 268(22):16746-53.
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  69. Abeijon C, Yanagisawa K, Mandon EC, Häusler A, Moremen K, Hirschberg CB, Robbins PW. Guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae. J Cell Biol. 1993 Jul; 122(2):307-23.
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  70. Häusler A, Ballou L, Ballou CE, Robbins PW. Yeast glycoprotein biosynthesis: MNT1 encodes an alpha-1,2-mannosyltransferase involved in O-glycosylation. Proc Natl Acad Sci U S A. 1992 Aug 1; 89(15):6846-50.
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  71. Robbins PW, Overbye K, Albright C, Benfield B, Pero J. Cloning and high-level expression of chitinase-encoding gene of Streptomyces plicatus. Gene. 1992 Feb 1; 111(1):69-76.
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  72. Häusler A, Robbins PW. Glycosylation in Saccharomyces cerevisiae: cloning and characterization of an alpha-1,2-mannosyltransferase structural gene. Glycobiology. 1992 Feb; 2(1):77-84.
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  73. Chen-Wu JL, Zwicker J, Bowen AR, Robbins PW. Expression of chitin synthase genes during yeast and hyphal growth phases of Candida albicans. Mol Microbiol. 1992 Feb; 6(4):497-502.
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  74. Bowen AR, Chen-Wu JL, Momany M, Young R, Szaniszlo PJ, Robbins PW. Classification of fungal chitin synthases. Proc Natl Acad Sci U S A. 1992 Jan 15; 89(2):519-23.
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  75. Moremen KW, Robbins PW. Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans. J Cell Biol. 1991 Dec; 115(6):1521-34.
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  76. Preuss D, Mulholland J, Kaiser CA, Orlean P, Albright C, Rose MD, Robbins PW, Botstein D. Structure of the yeast endoplasmic reticulum: localization of ER proteins using immunofluorescence and immunoelectron microscopy. Yeast. 1991 Dec; 7(9):891-911.
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  77. Kuranda MJ, Robbins PW. Chitinase is required for cell separation during growth of Saccharomyces cerevisiae. J Biol Chem. 1991 Oct 15; 266(29):19758-67.
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  78. Moremen KW, Touster O, Robbins PW. Novel purification of the catalytic domain of Golgi alpha-mannosidase II. Characterization and comparison with the intact enzyme. J Biol Chem. 1991 Sep 5; 266(25):16876-85.
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  79. Robbins PW. Genetic regulation of asparagine-linked oligosaccharide synthesis. Biochem Soc Trans. 1991 Aug; 19(3):642-5.
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  80. Yanagisawa K, Resnick D, Abeijon C, Robbins PW, Hirschberg CB. A guanosine diphosphatase enriched in Golgi vesicles of Saccharomyces cerevisiae. Purification and characterization. J Biol Chem. 1990 Nov 5; 265(31):19351-5.
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  81. Beck PJ, Orlean P, Albright C, Robbins PW, Gething MJ, Sambrook JF. The Saccharomyces cerevisiae DPM1 gene encoding dolichol-phosphate-mannose synthase is able to complement a glycosylation-defective mammalian cell line. Mol Cell Biol. 1990 Sep; 10(9):4612-22.
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  82. Au-Young J, Robbins PW. Isolation of a chitin synthase gene (CHS1) from Candida albicans by expression in Saccharomyces cerevisiae. Mol Microbiol. 1990 Feb; 4(2):197-207.
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  83. Albright CF, Orlean P, Robbins PW. A 13-amino acid peptide in three yeast glycosyltransferases may be involved in dolichol recognition. Proc Natl Acad Sci U S A. 1989 Oct; 86(19):7366-9.
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  84. Jackson BJ, Warren CD, Bugge B, Robbins PW. Synthesis of lipid-linked oligosaccharides in Saccharomyces cerevisiae: Man2GlcNAc2 and Man1GlcNAc2 are transferred from dolichol to protein in vivo. Arch Biochem Biophys. 1989 Jul; 272(1):203-9.
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  85. Orlean P, Albright C, Robbins PW. Cloning and sequencing of the yeast gene for dolichol phosphate mannose synthase, an essential protein. J Biol Chem. 1988 Nov 25; 263(33):17499-507.
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  86. Robbins PW, Albright C, Benfield B. Cloning and expression of a Streptomyces plicatus chitinase (chitinase-63) in Escherichia coli. J Biol Chem. 1988 Jan 5; 263(1):443-7.
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  87. Lu MC, Lien MH, Becker RE, Heine HC, Buggs AM, Lipovsek D, Gupta R, Robbins PW, Grosskinsky CM, Hubbard SC, et al. Genes for immunodominant protein antigens are highly homologous in Mycobacterium tuberculosis, Mycobacterium africanum, and the vaccine strain Mycobacterium bovis BCG. Infect Immun. 1987 Oct; 55(10):2378-82.
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  88. Bergh ML, Cepko CL, Wolf D, Robbins PW. Expression of the Saccharomyces cerevisiae glycoprotein invertase in mouse fibroblasts: glycosylation, secretion, and enzymatic activity. Proc Natl Acad Sci U S A. 1987 Jun; 84(11):3570-4.
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  89. Kuranda MJ, Robbins PW. Cloning and heterologous expression of glycosidase genes from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1987 May; 84(9):2585-9.
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  90. Kukuruzinska MA, Robbins PW. Protein glycosylation in yeast: transcript heterogeneity of the ALG7 gene. Proc Natl Acad Sci U S A. 1987 Apr; 84(8):2145-9.
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  91. Runge KW, Robbins PW. A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. Formation of a novel asparagine-linked oligosaccharide containing two glucose residues. J Biol Chem. 1986 Nov 25; 261(33):15582-90.
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  92. Bulawa CE, Slater M, Cabib E, Au-Young J, Sburlati A, Adair WL, Robbins PW. The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo. Cell. 1986 Jul 18; 46(2):213-25.
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  93. Mercurio AM, Robbins PW. Activation of mouse peritoneal macrophages alters the structure and surface expression of protein-bound lactosaminoglycans. J Immunol. 1985 Aug; 135(2):1305-12.
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  94. Kang MS, Elango N, Mattia E, Au-Young J, Robbins PW, Cabib E. Isolation of chitin synthetase from Saccharomyces cerevisiae. Purification of an enzyme by entrapment in the reaction product. J Biol Chem. 1984 Dec 10; 259(23):14966-72.
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  95. Hsieh P, Robbins PW. Regulation of asparagine-linked oligosaccharide processing. Oligosaccharide processing in Aedes albopictus mosquito cells. J Biol Chem. 1984 Feb 25; 259(4):2375-82.
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  96. Couto JR, Huffaker TC, Robbins PW. Cloning and expression in Escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway. J Biol Chem. 1984 Jan 10; 259(1):378-82.
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  97. Huffaker TC, Robbins PW. Yeast mutants deficient in protein glycosylation. Proc Natl Acad Sci U S A. 1983 Dec; 80(24):7466-70.
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  98. Sweeley CC, Ledonne NC, Robbins PW. Post-translational processing reactions involved in the biosynthesis of lysosomal alpha-N-acetylgalactosaminidase in cultured human fibroblasts. Arch Biochem Biophys. 1983 May; 223(1):158-65.
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  99. Hsieh P, Rosner MR, Robbins PW. Host-dependent variation of asparagine-linked oligosaccharides at individual glycosylation sites of Sindbis virus glycoproteins. J Biol Chem. 1983 Feb 25; 258(4):2548-54.
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  100. Snider MD, Huffaker TC, Couto JR, Robbins PW. Genetic and biochemical studies of asparagine-linked oligosaccharide assembly. Philos Trans R Soc Lond B Biol Sci. 1982 Dec 24; 300(1099):207-23.
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  101. Snider MD, Robbins PW. Transmembrane organization of protein glycosylation. Mature oligosaccharide-lipid is located on the luminal side of microsomes from Chinese hamster ovary cells. J Biol Chem. 1982 Jun 25; 257(12):6796-801.
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  102. Tulsiani DR, Hubbard SC, Robbins PW, Touster O. alpha-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes converting Man9 precursors to Man5 intermediates. J Biol Chem. 1982 Apr 10; 257(7):3660-8.
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  103. Huffaker TC, Robbins PW. Temperature-sensitive yeast mutants deficient in asparagine-linked glycosylation. J Biol Chem. 1982 Mar 25; 257(6):3203-10.
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  104. Rosner MR, Hubbard SC, Ivatt RJ, Robbins PW. N-asparagine-linked oligosaccharides: biosynthesis of the lipid-linked oligosaccharides. Methods Enzymol. 1982; 83:399-408.
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  105. Robbins PW, Wirth DF, Hering C. Expression of the Streptomyces enzyme endoglycosidase H in Escherichia coli. J Biol Chem. 1981 Oct 25; 256(20):10640-4.
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  106. Datema R, Lezica RP, Robbins PW, Schwarz RT. Deoxyglucose inhibition of protein glycosylation: effects of nucleotide deoxysugars on the formation of glucosylated lipid intermediates. Arch Biochem Biophys. 1981 Jan; 206(1):65-71.
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  107. Snider MD, Robbins PW. Synthesis and processing of asparagine-linked oligosaccharides of glycoproteins. Methods Cell Biol. 1981; 23:89-100.
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  108. Hubbard SC, Robbins PW. Synthesis of the N-linked oligosaccharides of glycoproteins. Assembly of the lipid-linked precursor oligosaccharide and its relation to protein synthesis in vivo. J Biol Chem. 1980 Dec 25; 255(24):11782-93.
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  109. Ivatt RJ, Hubbard SC, Robbins PW. Processing of cell surface glycoproteins in normal and transformed fibroblasts. Prog Clin Biol Res. 1980; 41:857-72.
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  110. Rosner MR, Grinna LS, Robbins PW. Differences in glycosylation patterns of closely related murine leukemia viruses. Proc Natl Acad Sci U S A. 1980 Jan; 77(1):67-71.
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  111. Grinna LS, Robbins PW. Glycoprotein biosynthesis. Rat liver microsomal glucosidases which process oligosaccharides. J Biol Chem. 1979 Sep 25; 254(18):8814-8.
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  112. Wirth DF, Lodish HF, Robbins PW. Requirements for the insertion of the Sindbis envelope glycoproteins into the endoplasmic reticulum membrane. J Cell Biol. 1979 Apr; 81(1):154-62.
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  113. Turco SJ, Robbins PW. Synthesis and function of carbohydrate units of cell surface glycoproteins. Ann N Y Acad Sci. 1978 Jun 20; 312:392-8.
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  114. Robbins PW, Hubbard SC, Turco SJ, Wirth DF. Proposal for a common oligosaccharide intermediate in the synthesis of membrane glycoproteins. Cell. 1977 Dec; 12(4):893-900.
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  115. Sakiyama H, Robbins PW. The effect of dibutyryl adenosine 3':5'-cyclic monophosphate on the synthesis of glycolipids by normal and transformed Nil cells. Arch Biochem Biophys. 1973 Jan; 154(1):407-14.
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  116. Dankert M, Wright A, Kelley WS, Robbins PW. Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesis. Arch Biochem Biophys. 1966 Sep 26; 116(1):425-35.
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