Keywords
Last Name

Lucia Rameh Plant, PhD

TitleResearch Associate Professor
InstitutionBoston University School of Medicine
DepartmentMedicine
DivisionEndocrinology, Diabetes & Nutrition
Address650 Albany St Evans Biomed Research Ctr
Boston MA 02118
Phone(617) 638-6012
ORCID ORCID Icon0000-0003-4257-542X
 Research Expertise & Professional Interests
Studies in the Rameh Lab (http://sites.bu.edu/rameh-lab/) are aimed at understanding how phosphoinositide lipids affect different aspects of the diabetes/obesity cycle. As regulator of many cellular processes, such as vesicle trafficking and hormone signaling, phosphoinositide kinases are promising targets in the treatment of these diseases. The Rameh Lab is particularly interested in determining the role of the phosphoinositide PtdIns-5-P in pancreatic beta cell function. Recent data from the Lab show that PtdIns-5-P 4-kinases can regulate nutrient-independent TORC1 signaling that activates basal protein translation. They are currently investigating how these can impact basal insulin production and secretion in the pre-diabetic state. The Rameh Lab uses state-of-the art high performance liquid chromatography (HPLC) coupled to flow scintillation analysis to measure cellular phosphoinositides in cultured cells. They have recently developed a unique method for PtdIns-5-P detection that will aid future research on the cellular function of this lipid.

 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Rameh LE, Deeney JT. Phosphoinositide signalling in type 2 diabetes: a ß-cell perspective. Biochem Soc Trans. 2016 Feb; 44(1):293-8. PMID: 26862218.
    View in: PubMed
  2. Sarkes DA, Rameh LE. Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions. Methods Mol Biol. 2016; 1376:213-27. PMID: 26552687.
    View in: PubMed
  3. Toker A, Rameh L. PIPPing on AKT1: How Many Phosphatases Does It Take to Turn off PI3K? Cancer Cell. 2015 Aug 10; 28(2):143-5. PMID: 26267528.
    View in: PubMed
  4. Mackey AM, Sarkes DA, Bettencourt I, Asara JM, Rameh LE. PIP4k? is a substrate for mTORC1 that maintains basal mTORC1 signaling during starvation. Sci Signal. 2014 Nov 04; 7(350):ra104. PMID: 25372051.
    View in: PubMed
  5. McNamara CW, Lee MC, Lim CS, Lim SH, Roland J, Nagle A, Simon O, Yeung BK, Chatterjee AK, McCormack SL, Manary MJ, Zeeman AM, Dechering KJ, Kumar TR, Henrich PP, Gagaring K, Ibanez M, Kato N, Kuhen KL, Fischli C, Rottmann M, Plouffe DM, Bursulaya B, Meister S, Rameh L, Trappe J, Haasen D, Timmerman M, Sauerwein RW, Suwanarusk R, Russell B, Renia L, Nosten F, Tully DC, Kocken CH, Glynne RJ, Bodenreider C, Fidock DA, Diagana TT, Winzeler EA. Targeting Plasmodium PI(4)K to eliminate malaria. Nature. 2013 Dec 12; 504(7479):248-53. PMID: 24284631.
    View in: PubMed
  6. Emerling BM, Hurov JB, Poulogiannis G, Tsukazawa KS, Choo-Wing R, Wulf GM, Bell EL, Shim HS, Lamia KA, Rameh LE, Bellinger G, Sasaki AT, Asara JM, Yuan X, Bullock A, Denicola GM, Song J, Brown V, Signoretti S, Cantley LC. Depletion of a putatively druggable class of phosphatidylinositol kinases inhibits growth of p53-null tumors. Cell. 2013 Nov 7; 155(4):844-57. PMID: 24209622.
    View in: PubMed
  7. Er EE, Mendoza MC, Mackey AM, Rameh LE, Blenis J. AKT facilitates EGFR trafficking and degradation by phosphorylating and activating PIKfyve. Sci Signal. 2013 Jun 11; 6(279):ra45. PMID: 23757022.
    View in: PubMed
  8. Oppelt A, Lobert VH, Haglund K, Mackey AM, Rameh LE, Liestøl K, Schink KO, Pedersen NM, Wenzel EM, Haugsten EM, Brech A, Rusten TE, Stenmark H, Wesche J. Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration. EMBO Rep. 2013 Jan; 14(1):57-64. PMID: 23154468.
    View in: PubMed
  9. Garcia-Cao I, Song MS, Hobbs RM, Laurent G, Giorgi C, de Boer VC, Anastasiou D, Ito K, Sasaki AT, Rameh L, Carracedo A, Vander Heiden MG, Cantley LC, Pinton P, Haigis MC, Pandolfi PP. Systemic elevation of PTEN induces a tumor-suppressive metabolic state. Cell. 2012 Mar 30; 149(1):49-62. PMID: 22401813.
    View in: PubMed
  10. Berger SB, Romero X, Ma C, Wang G, Faubion WA, Liao G, Compeer E, Keszei M, Rameh L, Wang N, Boes M, Regueiro JR, Reinecker HC, Terhorst C. SLAM is a microbial sensor that regulates bacterial phagosome functions in macrophages. Nat Immunol. 2010 Oct; 11(10):920-7. PMID: 20818396.
    View in: PubMed
  11. Furuya T, Kim M, Lipinski M, Li J, Kim D, Lu T, Shen Y, Rameh L, Yankner B, Tsai LH, Yuan J. Negative regulation of Vps34 by Cdk mediated phosphorylation. Mol Cell. 2010 May 28; 38(4):500-11. PMID: 20513426.
    View in: PubMed
  12. Sarkes D, Rameh LE. A novel HPLC-based approach makes possible the spatial characterization of cellular PtdIns5P and other phosphoinositides. Biochem J. 2010 May 27; 428(3):375-84. PMID: 20370717.
    View in: PubMed
  13. Poliseno L, Salmena L, Riccardi L, Fornari A, Song MS, Hobbs RM, Sportoletti P, Varmeh S, Egia A, Fedele G, Rameh L, Loda M, Pandolfi PP. Identification of the miR-106b~25 microRNA cluster as a proto-oncogenic PTEN-targeting intron that cooperates with its host gene MCM7 in transformation. Sci Signal. 2010 Apr 13; 3(117):ra29. PMID: 20388916.
    View in: PubMed
  14. Gewinner C, Wang ZC, Richardson A, Teruya-Feldstein J, Etemadmoghadam D, Bowtell D, Barretina J, Lin WM, Rameh L, Salmena L, Pandolfi PP, Cantley LC. Evidence that inositol polyphosphate 4-phosphatase type II is a tumor suppressor that inhibits PI3K signaling. Cancer Cell. 2009 Aug 4; 16(2):115-25. PMID: 19647222.
    View in: PubMed
  15. Demian DJ, Clugston SL, Foster MM, Rameh L, Sarkes D, Townson SA, Yang L, Zhang M, Charlton ME. High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases. J Biomol Screen. 2009 Aug; 14(7):838-44. PMID: 19641220.
    View in: PubMed
  16. Vasudevan KM, Barbie DA, Davies MA, Rabinovsky R, McNear CJ, Kim JJ, Hennessy BT, Tseng H, Pochanard P, Kim SY, Dunn IF, Schinzel AC, Sandy P, Hoersch S, Sheng Q, Gupta PB, Boehm JS, Reiling JH, Silver S, Lu Y, Stemke-Hale K, Dutta B, Joy C, Sahin AA, Gonzalez-Angulo AM, Lluch A, Rameh LE, Jacks T, Root DE, Lander ES, Mills GB, Hahn WC, Sellers WR, Garraway LA. AKT-independent signaling downstream of oncogenic PIK3CA mutations in human cancer. Cancer Cell. 2009 Jul 7; 16(1):21-32. PMID: 19573809.
    View in: PubMed
  17. Mallo GV, Espina M, Smith AC, Terebiznik MR, Alemán A, Finlay BB, Rameh LE, Grinstein S, Brumell JH. SopB promotes phosphatidylinositol 3-phosphate formation on Salmonella vacuoles by recruiting Rab5 and Vps34. J Cell Biol. 2008 Aug 25; 182(4):741-52. PMID: 18725540.
    View in: PubMed
  18. Mandl A, Sarkes D, Carricaburu V, Jung V, Rameh L. Serum withdrawal-induced accumulation of phosphoinositide 3-kinase lipids in differentiating 3T3-L6 myoblasts: distinct roles for Ship2 and PTEN. Mol Cell Biol. 2007 Dec; 27(23):8098-112. PMID: 17893321.
    View in: PubMed
  19. Mason D, Mallo GV, Terebiznik MR, Payrastre B, Finlay BB, Brumell JH, Rameh L, Grinstein S. Alteration of epithelial structure and function associated with PtdIns(4,5)P2 degradation by a bacterial phosphatase. J Gen Physiol. 2007 Apr; 129(4):267-83. PMID: 17389247.
    View in: PubMed
  20. Mitra P, Zhang Y, Rameh LE, Ivshina MP, McCollum D, Nunnari JJ, Hendricks GM, Kerr ML, Field SJ, Cantley LC, Ross AH. A novel phosphatidylinositol(3,4,5)P3 pathway in fission yeast. J Cell Biol. 2004 Jul 19; 166(2):205-11. PMID: 15249580.
    View in: PubMed
  21. Lamia KA, Peroni OD, Kim YB, Rameh LE, Kahn BB, Cantley LC. Increased insulin sensitivity and reduced adiposity in phosphatidylinositol 5-phosphate 4-kinase beta-/- mice. Mol Cell Biol. 2004 Jun; 24(11):5080-7. PMID: 15143198.
    View in: PubMed
  22. Rufo PA, Lin PW, Andrade A, Jiang L, Rameh L, Flexner C, Alper SL, Lencer WI. Diarrhea-associated HIV-1 APIs potentiate muscarinic activation of Cl- secretion by T84 cells via prolongation of cytosolic Ca2+ signaling. Am J Physiol Cell Physiol. 2004 May; 286(5):C998-C1008. PMID: 15075198.
    View in: PubMed
  23. Chang JD, Field SJ, Rameh LE, Carpenter CL, Cantley LC. Identification and characterization of a phosphoinositide phosphate kinase homolog. J Biol Chem. 2004 Mar 19; 279(12):11672-9. PMID: 14701839.
    View in: PubMed
  24. Carricaburu V, Lamia KA, Lo E, Favereaux L, Payrastre B, Cantley LC, Rameh LE. The phosphatidylinositol (PI)-5-phosphate 4-kinase type II enzyme controls insulin signaling by regulating PI-3,4,5-trisphosphate degradation. Proc Natl Acad Sci U S A. 2003 Aug 19; 100(17):9867-72. PMID: 12897244.
    View in: PubMed
  25. Field SJ, Lamia KA, Rameh LE, Cantley LC. Lipid research picks up speed on the slopes of Taos. Dev Cell. 2002 Apr; 2(4):407-10. PMID: 11970891.
    View in: PubMed
  26. Vieira OV, Botelho RJ, Rameh L, Brachmann SM, Matsuo T, Davidson HW, Schreiber A, Backer JM, Cantley LC, Grinstein S. Distinct roles of class I and class III phosphatidylinositol 3-kinases in phagosome formation and maturation. J Cell Biol. 2001 Oct 1; 155(1):19-25. PMID: 11581283.
    View in: PubMed
  27. Fruman DA, Rameh LE, Cantley LC. Phosphoinositide binding domains: embracing 3-phosphate. Cell. 1999 Jun 25; 97(7):817-20. PMID: 10399908.
    View in: PubMed
  28. Rameh LE, Cantley LC. The role of phosphoinositide 3-kinase lipid products in cell function. J Biol Chem. 1999 Mar 26; 274(13):8347-50. PMID: 10085060.
    View in: PubMed
  29. Rameh LE, Rhee SG, Spokes K, Kazlauskas A, Cantley LC, Cantley LG. Phosphoinositide 3-kinase regulates phospholipase Cgamma-mediated calcium signaling. J Biol Chem. 1998 Sep 11; 273(37):23750-7. PMID: 9726983.
    View in: PubMed
  30. Tolias KF, Rameh LE, Ishihara H, Shibasaki Y, Chen J, Prestwich GD, Cantley LC, Carpenter CL. Type I phosphatidylinositol-4-phosphate 5-kinases synthesize the novel lipids phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 5-phosphate. J Biol Chem. 1998 Jul 17; 273(29):18040-6. PMID: 9660759.
    View in: PubMed
  31. Klarlund JK, Rameh LE, Cantley LC, Buxton JM, Holik JJ, Sakelis C, Patki V, Corvera S, Czech MP. Regulation of GRP1-catalyzed ADP ribosylation factor guanine nucleotide exchange by phosphatidylinositol 3,4,5-trisphosphate. J Biol Chem. 1998 Jan 23; 273(4):1859-62. PMID: 9442017.
    View in: PubMed
  32. Rameh LE, Tolias KF, Duckworth BC, Cantley LC. A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate. Nature. 1997 Nov 13; 390(6656):192-6. PMID: 9367159.
    View in: PubMed
  33. Rameh LE, Arvidsson Ak, Carraway KL, Couvillon AD, Rathbun G, Crompton A, VanRenterghem B, Czech MP, Ravichandran KS, Burakoff SJ, Wang DS, Chen CS, Cantley LC. A comparative analysis of the phosphoinositide binding specificity of pleckstrin homology domains. J Biol Chem. 1997 Aug 29; 272(35):22059-66. PMID: 9268346.
    View in: PubMed
  34. Volker JL, Rameh LE, Zhu Q, DeCaprio J, Hansen U. Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity. Genes Dev. 1997 Jun 1; 11(11):1435-46. PMID: 9192871.
    View in: PubMed
  35. Bachelot C, Rameh L, Parsons T, Cantley LC. Association of phosphatidylinositol 3-kinase, via the SH2 domains of p85, with focal adhesion kinase in polyoma middle t-transformed fibroblasts. Biochim Biophys Acta. 1996 Mar 27; 1311(1):45-52. PMID: 8603102.
    View in: PubMed
  36. Rameh LE, Chen CS, Cantley LC. Phosphatidylinositol (3,4,5)P3 interacts with SH2 domains and modulates PI 3-kinase association with tyrosine-phosphorylated proteins. Cell. 1995 Dec 1; 83(5):821-30. PMID: 8521499.
    View in: PubMed
  37. Yeh JI, Gulve EA, Rameh L, Birnbaum MJ. The effects of wortmannin on rat skeletal muscle. Dissociation of signaling pathways for insulin- and contraction-activated hexose transport. J Biol Chem. 1995 Feb 3; 270(5):2107-11. PMID: 7836438.
    View in: PubMed
  38. Jhun BH, Rose DW, Seely BL, Rameh L, Cantley L, Saltiel AR, Olefsky JM. Microinjection of the SH2 domain of the 85-kilodalton subunit of phosphatidylinositol 3-kinase inhibits insulin-induced DNA synthesis and c-fos expression. Mol Cell Biol. 1994 Nov; 14(11):7466-75. PMID: 7935461.
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  39. Rameh LE, Armelin MC. Downregulation of JE and KC genes by glucocorticoids does not prevent the G0----G1 transition in BALB/3T3 cells. Mol Cell Biol. 1992 Oct; 12(10):4612-21. PMID: 1406651.
    View in: PubMed
  40. Rameh LE, Armelin MC. T antigens' role in polyomavirus transformation: c-myc but not c-fos or c-jun expression is a target for middle T. Oncogene. 1991 Jun; 6(6):1049-56. PMID: 1648700.
    View in: PubMed
  41. Costanzi E, Rameh LE, Joazeiro CA, Armelin MC. Generation of cell lines to study the role played by oncogenes and anti-oncogenes in cell proliferation control. Braz J Med Biol Res. 1990; 23(9):795-9. PMID: 2101319.
    View in: PubMed
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