Clint Makino | Profiles RNS

Clint Makino, PhD

Associate Professor

Boston University Chobanian & Avedisian School of Medicine

Pharmacology, Physiology & Biophysics

PhD, Florida State University - Tallahassee
BS, Duke University

Websites

	Pharmacology, Physiology & Biophysics Faculty
	LinkedIn
	ResearchGate
	Loop Frontiers

ORCID : 0000-0002-6005-9069

Dr. Clint Makino completed his PhD at Florida State University and postdoctoral studies at Stanford University School of Medicine. He is currently an Associate Professor of Physiology and Biophysics at Chobanian and Avedisian School of Medicine. He has published more than 50 journal papers and book chapters and serves on the editorial board of Frontiers in Molecular Neuroscience.

Graduate Faculty (Primary Mentor of Grad Students)
Boston University Chobanian & Avedisian School of Medicine, Graduate Medical Sciences

BU Research
NIH RePORTER Research

Rod outer segment structure: determinants and its effect on the photon response
09/01/2020 - 06/30/2024 (PI)
NIH/National Eye Institute
5R01EY031702-04

Bridging across scales to model cone phototransduction
08/01/2018 - 07/31/2021 (Subcontract PI)
Vanderbilt University National Science Fdn
DMS-1812601

Modulation of Visual Transduction and Retinal Disease by Bicarbonate
05/01/2016 - 03/31/2019 (PI)
NIH/National Eye Institute
5R01EY023980-05

Title

Yr	Title	Project-Sub Proj	Pubs
2023	Rod outer segment structure: determinants and its effect on the photon response	5R01EY031702-04
2022	Rod outer segment structure: determinants and its effect on the photon response	3R01EY031702-02S1
2022	Rod outer segment structure: determinants and its effect on the photon response	5R01EY031702-03
2021	Rod outer segment structure: determinants and its effect on the photon response	5R01EY031702-02
2020	Rod outer segment structure: determinants and its effect on the photon response	1R01EY031702-01
2017	Modulation of Visual Transduction and Retinal Disease by Bicarbonate	5R01EY023980-05	5
2016	Modulation of Visual Transduction and Retinal Disease by Bicarbonate	5R01EY023980-04	5
2015	Modulation of Visual Transduction and Retinal Disease by Bicarbonate	7R01EY023980-03	5
2015	Modulation of Visual Transduction and Retinal Disease by Bicarbonate	5R01EY023980-02	5
2014	Modulation of Visual Transduction and Retinal Disease by Bicarbonate	1R01EY023980-01A1	5

Showing 10 of 30 results. Show All Results

Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.

iCite Analysis Copy PMIDs To Clipboard

Adhikari RD, Kossoff AM, Cornwall MC, Makino CL. Bicarbonate boosts flash response amplitude to augment absolute sensitivity and extend dynamic range in murine retinal rods. Front Mol Neurosci. 2023; 16:1125006.View Related Profiles.

Makino, Clint
Cornwall, M.

PMID: 37122625; PMCID: PMC10140344; DOI: 10.3389/fnmol.2023.1125006;

Sharma RK, Pandey KN, Duda T, Makino CL. Editorial: Multi-limbed membrane guanylate cyclase cellular signaling pathways. Front Mol Neurosci. 2023; 16:1185637. PMID: 37122624; PMCID: PMC10130573; DOI: 10.3389/fnmol.2023.1185637;

Caruso G, Klaus C, Hamm HE, Gurevich VV, Bisegna P, Andreucci D, DiBenedetto E, Makino CL. Pepperberg plot: Modeling flash response saturation in retinal rods of mouse. Front Mol Neurosci. 2022; 15:1054449. PMID: 36710929; PMCID: PMC9880052; DOI: 10.3389/fnmol.2022.1054449;

Geva P, Caruso G, Klaus C, Hamm HE, Gurevich VV, DiBenedetto E, Makino CL. Effects of cell size and bicarbonate on single photon response variability in retinal rods. Front Mol Neurosci. 2022; 15:1050545.View Related Profiles.

Makino, Clint
Geva, Polina

PMID: 36590910; PMCID: PMC9796569; DOI: 10.3389/fnmol.2022.1050545;

Klaus C, Caruso G, Gurevich VV, Hamm HE, Makino CL, DiBenedetto E. Phototransduction in retinal cones: Analysis of parameter importance. PLoS One. 2021; 16(10):e0258721. PMID: 34710119; PMCID: PMC8553137; DOI: 10.1371/journal.pone.0258721;

Caruso G, Klaus CJ, Hamm HE, Gurevich VV, Makino CL, DiBenedetto E. Position of rhodopsin photoisomerization on the disk surface confers variability to the rising phase of the single photon response in vertebrate rod photoreceptors. PLoS One. 2020; 15(10):e0240527. PMID: 33052986; PMCID: PMC7556485; DOI: 10.1371/journal.pone.0240527;

Caruso G, Gurevich VV, Klaus C, Hamm H, Makino CL, DiBenedetto E. Local, nonlinear effects of cGMP and Ca2+ reduce single photon response variability in retinal rods. PLoS One. 2019; 14(12):e0225948. PMID: 31805112; PMCID: PMC6894879; DOI: 10.1371/journal.pone.0225948;

Makino CL, Duda T, Pertzev A, Isayama T, Geva P, Sandberg MA, Sharma RK. Modes of Accessing Bicarbonate for the Regulation of Membrane Guanylate Cyclase (ROS-GC) in Retinal Rods and Cones. eNeuro. 2019 Jan-Feb; 6(1).View Related Profiles.

Makino, Clint
Geva, Polina

PMID: 30783616; PMCID: PMC6378327; DOI: 10.1523/ENEURO.0393-18.2019;

Makino CL, Duda T, Pertzev A, Sharma RK. Experimental Approaches for Defining the Role of the Ca2+-Modulated ROS-GC System in Retinal Rods of Mouse. Methods Mol Biol. 2018; 1753:129-158. PMID: 29564786

Sharma RK, Duda T, Makino CL. Integrative Signaling Networks of Membrane Guanylate Cyclases: Biochemistry and Physiology. Front Mol Neurosci. 2016; 9:83. PMID: 27695398; PMCID: PMC5023690; DOI: 10.3389/fnmol.2016.00083;

Showing 10 of 58 results. Show More

Duda T, Pertzev A, Makino CL, Sharma RK. Bicarbonate and Ca(2+) Sensing Modulators Activate Photoreceptor ROS-GC1 Synergistically. Front Mol Neurosci. 2016; 9:5. PMID: 26858600; PMCID: PMC4729890; DOI: 10.3389/fnmol.2016.00005;

Duda T, Wen XH, Isayama T, Sharma RK, Makino CL. Bicarbonate Modulates Photoreceptor Guanylate Cyclase (ROS-GC) Catalytic Activity. J Biol Chem. 2015 Apr 24; 290(17):11052-60. PMID: 25767116; PMCID: PMC4409265; DOI: 10.1074/jbc.M115.650408;

Sharma RK, Baehr W, Makino CL, Duda T. Ca(2+) and Ca(2+)-interlocked membrane guanylate cyclase signal modulation of neuronal and cardiovascular signal transduction. Front Mol Neurosci. 2015; 8:7. PMID: 25798085; PMCID: PMC4351612; DOI: 10.3389/fnmol.2015.00007;

Isayama T, Chen Y, Kono M, Fabre E, Slavsky M, DeGrip WJ, Ma JX, Crouch RK, Makino CL. Coexpression of three opsins in cone photoreceptors of the salamander Ambystoma tigrinum. J Comp Neurol. 2014 Jul 01; 522(10):2249-65. PMID: 24374736; PMCID: PMC3997598; DOI: 10.1002/cne.23531;

Wen XH, Dizhoor AM, Makino CL. Membrane guanylyl cyclase complexes shape the photoresponses of retinal rods and cones. Front Mol Neurosci. 2014; 7:45. PMID: 24917784; PMCID: PMC4040495; DOI: 10.3389/fnmol.2014.00045;

Sharma RK, Makino CL, Hicks D, Duda T. ROS-GC interlocked Ca(2+)-sensor S100B protein signaling in cone photoreceptors: review. Front Mol Neurosci. 2014; 7:21. PMID: 24723847; PMCID: PMC3972482; DOI: 10.3389/fnmol.2014.00021;

Makino CL, Wen XH, Olshevskaya EV, Peshenko IV, Savchenko AB, Dizhoor AM. Enzymatic relay mechanism stimulates cyclic GMP synthesis in rod photoresponse: biochemical and physiological study in guanylyl cyclase activating protein 1 knockout mice. PLoS One. 2012; 7(10):e47637. PMID: 23082185; PMCID: PMC3474714; DOI: 10.1371/journal.pone.0047637;

Makino CL. Easy does it when bleaching isolated mouse rods. J Physiol. 2012 Jun 01; 590(11):2551-2. PMID: 22787167; PMCID: PMC3424712; DOI: 10.1113/jphysiol.2012.233643;

Makino CL, Wen XH, Michaud NA, Covington HI, DiBenedetto E, Hamm HE, Lem J, Caruso G. Rhodopsin expression level affects rod outer segment morphology and photoresponse kinetics. PLoS One. 2012; 7(5):e37832. PMID: 22662234; PMCID: PMC3360601; DOI: 10.1371/journal.pone.0037832;

Wen XH, Duda T, Pertzev A, Venkataraman V, Makino CL, Sharma RK. S100B serves as a Ca(2+) sensor for ROS-GC1 guanylate cyclase in cones but not in rods of the murine retina. Cell Physiol Biochem. 2012; 29(3-4):417-30. PMID: 22508049; PMCID: PMC3434333; DOI: 10.1159/000338496;

Miyazono S, Isayama T, Delori FC, Makino CL. Vitamin A activates rhodopsin and sensitizes it to ultraviolet light. Vis Neurosci. 2011 Nov; 28(6):485-97. PMID: 22192505; PMCID: PMC3601037; DOI: 10.1017/S0952523811000423;

Makino CL, Riley CK, Looney J, Crouch RK, Okada T. Binding of more than one retinoid to visual opsins. Biophys J. 2010 Oct 06; 99(7):2366-73. PMID: 20923672; PMCID: PMC3042582; DOI: 10.1016/j.bpj.2010.08.003;

Liu X, Pawlyk BS, Adamian M, Olshevskaya EV, Dizhoor AM, Makino CL, Li T. Increased light exposure alleviates one form of photoreceptor degeneration marked by elevated calcium in the dark. PLoS One. 2009 Dec 24; 4(12):e8438. PMID: 20041177; PMCID: PMC2793020; DOI: 10.1371/journal.pone.0008438;

Isayama T, McCabe England SL, Crouch RK, Zimmerman AL, Makino CL. Beta-ionone activates and bleaches visual pigment in salamander photoreceptors. Vis Neurosci. 2009 May-Jun; 26(3):267-74. PMID: 19500430; PMCID: PMC3220273; DOI: 10.1017/S0952523809090105;

Wen XH, Shen L, Brush RS, Michaud N, Al-Ubaidi MR, Gurevich VV, Hamm HE, Lem J, Dibenedetto E, Anderson RE, Makino CL. Overexpression of rhodopsin alters the structure and photoresponse of rod photoreceptors. Biophys J. 2009 Feb; 96(3):939-50. PMID: 19186132; PMCID: PMC2716671; DOI: 10.1016/j.bpj.2008.10.016;

Makino CL, Peshenko IV, Wen XH, Olshevskaya EV, Barrett R, Dizhoor AM. A role for GCAP2 in regulating the photoresponse. Guanylyl cyclase activation and rod electrophysiology in GUCA1B knock-out mice. J Biol Chem. 2008 Oct 24; 283(43):29135-43. PMID: 18723510; PMCID: PMC2570858; DOI: 10.1074/jbc.M804445200;

Yang J, Pawlyk B, Wen XH, Adamian M, Soloviev M, Michaud N, Zhao Y, Sandberg MA, Makino CL, Li T. Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminals. Hum Mol Genet. 2007 May 01; 16(9):1017-29. PMID: 17341488

Isayama T, Chen Y, Kono M, Degrip WJ, Ma JX, Crouch RK, Makino CL. Differences in the pharmacological activation of visual opsins. Vis Neurosci. 2006 Nov-Dec; 23(6):899-908. PMID: 17266782

Isayama T, Alexeev D, Makino CL, Washington I, Nakanishi K, Turro NJ. An accessory chromophore in red vision. Nature. 2006 Oct 12; 443(7112):649. PMID: 17035994

He Q, Alexeev D, Estevez ME, McCabe SL, Calvert PD, Ong DE, Cornwall MC, Zimmerman AL, Makino CL. Cyclic nucleotide-gated ion channels in rod photoreceptors are protected from retinoid inhibition. J Gen Physiol. 2006 Oct; 128(4):473-85.View Related Profiles.

Makino, Clint
Cornwall, M.

PMID: 17001087; PMCID: PMC2151575; DOI: 10.1085/jgp.200609619;

Makino CL, Wen XH, Michaud N, Peshenko IV, Pawlyk B, Brush RS, Soloviev M, Liu X, Woodruff ML, Calvert PD, Savchenko AB, Anderson RE, Fain GL, Li T, Sandberg MA, Dizhoor AM. Effects of low AIPL1 expression on phototransduction in rods. Invest Ophthalmol Vis Sci. 2006 May; 47(5):2185-94. PMID: 16639031

Yang J, Gao J, Adamian M, Wen XH, Pawlyk B, Zhang L, Sanderson MJ, Zuo J, Makino CL, Li T. The ciliary rootlet maintains long-term stability of sensory cilia. Mol Cell Biol. 2005 May; 25(10):4129-37. PMID: 15870283; PMCID: PMC1087714; DOI: 10.1128/MCB.25.10.4129-4137.2005;

Wang Z, Wen XH, Ablonczy Z, Crouch RK, Makino CL, Lem J. Enhanced shutoff of phototransduction in transgenic mice expressing palmitoylation-deficient rhodopsin. J Biol Chem. 2005 Jul 01; 280(26):24293-300. PMID: 15851469; PMCID: PMC2247473; DOI: 10.1074/jbc.M502588200;

Liu X, Bulgakov OV, Wen XH, Woodruff ML, Pawlyk B, Yang J, Fain GL, Sandberg MA, Makino CL, Li T. AIPL1, the protein that is defective in Leber congenital amaurosis, is essential for the biosynthesis of retinal rod cGMP phosphodiesterase. Proc Natl Acad Sci U S A. 2004 Sep 21; 101(38):13903-8. PMID: 15365173; PMCID: PMC518851

Olshevskaya EV, Calvert PD, Woodruff ML, Peshenko IV, Savchenko AB, Makino CL, Ho YS, Fain GL, Dizhoor AM. The Y99C mutation in guanylyl cyclase-activating protein 1 increases intracellular Ca2+ and causes photoreceptor degeneration in transgenic mice. J Neurosci. 2004 Jul 07; 24(27):6078-85. PMID: 15240799

Makino CL, Dodd RL, Chen J, Burns ME, Roca A, Simon MI, Baylor DA. Recoverin regulates light-dependent phosphodiesterase activity in retinal rods. J Gen Physiol. 2004 Jun; 123(6):729-41. PMID: 15173221; PMCID: PMC2234569

Makino CL, Wen XH, Lem J. Piecing together the timetable for visual transduction with transgenic animals. Curr Opin Neurobiol. 2003 Aug; 13(4):404-12. PMID: 12965286

Calvert PD, Govardovskii VI, Arshavsky VY, Makino CL. Two temporal phases of light adaptation in retinal rods. J Gen Physiol. 2002 Feb; 119(2):129-45. PMID: 11815664; PMCID: PMC2233805

Calvert PD, Makino CL. The time course of light adaptation in vertebrate retinal rods. Adv Exp Med Biol. 2002; 514:37-60. PMID: 12596914

Ma J, Znoiko S, Othersen KL, Ryan JC, Das J, Isayama T, Kono M, Oprian DD, Corson DW, Cornwall MC, Cameron DA, Harosi FI, Makino CL, Crouch RK. A visual pigment expressed in both rod and cone photoreceptors. Neuron. 2001 Nov 8; 32(3):451-61.View Related Profiles.

Makino, Clint
Cornwall, M.

PMID: 11709156

Sieving PA, Fowler ML, Bush RA, Machida S, Calvert PD, Green DG, Makino CL, McHenry CL. Constitutive "light" adaptation in rods from G90D rhodopsin: a mechanism for human congenital nightblindness without rod cell loss. J Neurosci. 2001 Aug 01; 21(15):5449-60. PMID: 11466416

Calvert PD, Govardovskii VI, Krasnoperova N, Anderson RE, Lem J, Makino CL. Membrane protein diffusion sets the speed of rod phototransduction. Nature. 2001 May 03; 411(6833):90-4. PMID: 11333983

Calvert PD, Krasnoperova NV, Lyubarsky AL, Isayama T, Nicoló M, Kosaras B, Wong G, Gannon KS, Margolskee RF, Sidman RL, Pugh EN, Makino CL, Lem J. Phototransduction in transgenic mice after targeted deletion of the rod transducin alpha -subunit. Proc Natl Acad Sci U S A. 2000 Dec 05; 97(25):13913-8. PMID: 11095744; PMCID: PMC17675

Makino CL, Groesbeek M, Lugtenburg J, Baylor DA. Spectral tuning in salamander visual pigments studied with dihydroretinal chromophores. Biophys J. 1999 Aug; 77(2):1024-35. PMID: 10423447; PMCID: PMC1300393

Lem J, Krasnoperova NV, Calvert PD, Kosaras B, Cameron DA, Nicolò M, Makino CL, Sidman RL. Morphological, physiological, and biochemical changes in rhodopsin knockout mice. Proc Natl Acad Sci U S A. 1999 Jan 19; 96(2):736-41. PMID: 9892703; PMCID: PMC15206

Xu J, Dodd RL, Makino CL, Simon MI, Baylor DA, Chen J. Prolonged photoresponses in transgenic mouse rods lacking arrestin. Nature. 1997 Oct 02; 389(6650):505-9. PMID: 9333241

Lem J, Makino CL. Phototransduction in transgenic mice. Curr Opin Neurobiol. 1996 Aug; 6(4):453-8. PMID: 8794096

Makino CL, Dodd RL. Multiple visual pigments in a photoreceptor of the salamander retina. J Gen Physiol. 1996 Jul; 108(1):27-34. PMID: 8817382; PMCID: PMC2229295

Chen J, Makino CL, Peachey NS, Baylor DA, Simon MI. Mechanisms of rhodopsin inactivation in vivo as revealed by a COOH-terminal truncation mutant. Science. 1995 Jan 20; 267(5196):374-7. PMID: 7824934

Sung CH, Makino C, Baylor D, Nathans J. A rhodopsin gene mutation responsible for autosomal dominant retinitis pigmentosa results in a protein that is defective in localization to the photoreceptor outer segment. J Neurosci. 1994 Oct; 14(10):5818-33. PMID: 7523628

Raport CJ, Lem J, Makino C, Chen CK, Fitch CL, Hobson A, Baylor D, Simon MI, Hurley JB. Downregulation of cGMP phosphodiesterase induced by expression of GTPase-deficient cone transducin in mouse rod photoreceptors. Invest Ophthalmol Vis Sci. 1994 Jun; 35(7):2932-47. PMID: 8206711

Makino CL, Taylor WR, Baylor DA. Rapid charge movements and photosensitivity of visual pigments in salamander rods and cones. J Physiol. 1991 Oct; 442:761-80. PMID: 1818565; PMCID: PMC1179914

Makino CL, Howard LN, Williams TP. Axial gradients of rhodopsin in light-exposed retinal rods of the toad. J Gen Physiol. 1990 Dec; 96(6):1199-220. PMID: 2126801; PMCID: PMC2229032

Makino CL, Kraft TW, Mathies RA, Lugtenburg J, Miley ME, van der Steen R, Baylor DA. Effects of modified chromophores on the spectral sensitivity of salamander, squirrel and macaque cones. J Physiol. 1990 May; 424:545-60. PMID: 2391661; PMCID: PMC1189828

Kraft TW, Makino CL, Mathies RA, Lugtenburg J, Schnapf JL, Baylor DA. Cone excitations and color vision. Cold Spring Harb Symp Quant Biol. 1990; 55:635-41. PMID: 2132843

Makino CL, Howard LN, Williams TP. Intracellular topography of rhodopsin bleaching. Science. 1987 Dec 18; 238(4834):1716-7. PMID: 3686009

Thompson JM, Makino CL, Whitaker JR, Joseph JA. Age-related decrease in apomorphine modulation of acetylcholine release from rat striatal slices. Brain Res. 1984 May 07; 299(1):169-73. PMID: 6722565

Schiffman SS, Lindley MG, Clark TB, Makino C. Molecular mechanism of sweet taste: relationship of hydrogen bonding to taste sensitivity for both young and elderly. Neurobiol Aging. 1981; 2(3):173-85. PMID: 7312095

This graph shows the total number of publications by year, by first, middle/unknown, or last author.

Bar chart showing 58 publications over 32 distinct years, with a maximum of 4 publications in 2006 and 2012

Bar chart showing 58 publications over 32 distinct years, with a maximum of 4 publications in 2006 and 2012

Year	Publications
1981	1
1984	1
1987	1
1990	3
1991	1
1994	2
1995	1
1996	2
1997	1
1999	2
2000	1
2001	3
2002	2
2003	1
2004	3
2005	2
2006	4
2007	1
2008	1
2009	3
2010	1
2011	1
2012	4
2014	3
2015	2
2016	2
2018	1
2019	2
2020	1
2021	1
2022	1
2023	3

1998 Florida State University: Distinguished Alumnus Award

1989 FASEB Young Investigator Award

1982-1987 Patricia Roberts Harris Fellowship

Contact for Mentoring:

700 Albany Street
Boston MA 02118
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