Keywords
Last Name

Christopher W. Akey, PhD

TitleProfessor
InstitutionBoston University School of Medicine
DepartmentPhysiology & Biophysics
Address700 Albany St
Boston MA 02118
Phone(617) 638-4051
ORCID ORCID Icon0000-0002-3059-3121
Other Positions
TitleGraduate Faculty (Primary Mentor of Grad Students)
InstitutionBoston University School of Medicine, Graduate Medical Sciences

 Research Expertise & Professional Interests
We are studying the structure and function of macromolecular machines and chaperones. In our work, we use biochemistry and molecular biology coupled with structural electron microscopy, X-ray crystallography and molecular modeling. This hybrid approach allows us to create snapshots of these large assemblies and thus, to infer function from form.

 Publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Park E, Ménétret JF, Gumbart JC, Ludtke SJ, Li W, Whynot A, Rapoport TA, Akey CW. Structure of the SecY channel during initiation of protein translocation. Nature. 2014 Feb 6; 506(7486):102-6.
    View in: PubMed
  2. D'Brot A, Chen P, Vaishnav M, Yuan S, Akey CW, Abrams JM. Tango7 directs cellular remodeling by the Drosophila apoptosome. Genes Dev. 2013 Aug 1; 27(15):1650-5.
    View in: PubMed
  3. Farelli JD, Gumbart JC, Akey IV, Hempstead A, Amyot W, Head JF, McKnight CJ, Isberg RR, Akey CW. IcmQ in the Type 4b secretion system contains an NAD+ binding domain. Structure. 2013 Aug 6; 21(8):1361-73.
    View in: PubMed
  4. Yuan S, Akey CW. Apoptosome structure, assembly, and procaspase activation. Structure. 2013 Apr 2; 21(4):501-15.
    View in: PubMed
  5. Yuan S, Topf M, Reubold TF, Eschenburg S, Akey CW. Changes in Apaf-1 conformation that drive apoptosome assembly. Biochemistry. 2013 Apr 2; 52(13):2319-27.
    View in: PubMed
  6. Platonova O, Akey IV, Head JF, Akey CW. Crystal structure and function of human nucleoplasmin (npm2): a histone chaperone in oocytes and embryos. Biochemistry. 2011 Sep 20; 50(37):8078-89.
    View in: PubMed
  7. Yuan S, Yu X, Asara JM, Heuser JE, Ludtke SJ, Akey CW. The holo-apoptosome: activation of procaspase-9 and interactions with caspase-3. Structure. 2011 Aug 10; 19(8):1084-96.
    View in: PubMed
  8. Yuan S, Yu X, Topf M, Dorstyn L, Kumar S, Ludtke SJ, Akey CW. Structure of the Drosophila apoptosome at 6.9 å resolution. Structure. 2011 Jan 12; 19(1):128-40.
    View in: PubMed
  9. Akey CW. The NPC-transporter, a ghost in the machine. Structure. 2010 Oct 13; 18(10):1230-2.
    View in: PubMed
  10. Yuan S, Yu X, Topf M, Ludtke SJ, Wang X, Akey CW. Structure of an apoptosome-procaspase-9 CARD complex. Structure. 2010 May 12; 18(5):571-83.
    View in: PubMed
  11. Raychaudhury S, Farelli JD, Montminy TP, Matthews M, Ménétret JF, Duménil G, Roy CR, Head JF, Isberg RR, Akey CW. Structure and function of interacting IcmR-IcmQ domains from a type IVb secretion system in Legionella pneumophila. Structure. 2009 Apr 15; 17(4):590-601.
    View in: PubMed
  12. Ménétret JF, Hegde RS, Aguiar M, Gygi SP, Park E, Rapoport TA, Akey CW. Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome. Structure. 2008 Jul; 16(7):1126-37.
    View in: PubMed
  13. Chandramouli P, Topf M, Ménétret JF, Eswar N, Cannone JJ, Gutell RR, Sali A, Akey CW. Structure of the mammalian 80S ribosome at 8.7 A resolution. Structure. 2008 Apr; 16(4):535-48.
    View in: PubMed
  14. Ménétret JF, Schaletzky J, Clemons WM, Osborne AR, Skånland SS, Denison C, Gygi SP, Kirkpatrick DS, Park E, Ludtke SJ, Rapoport TA, Akey CW. Ribosome binding of a single copy of the SecY complex: implications for protein translocation. Mol Cell. 2007 Dec 28; 28(6):1083-92.
    View in: PubMed
  15. Yu X, Wang L, Acehan D, Wang X, Akey CW. Three-dimensional structure of a double apoptosome formed by the Drosophila Apaf-1 related killer. J Mol Biol. 2006 Jan 20; 355(3):577-89.
    View in: PubMed
  16. Yu X, Acehan D, Ménétret JF, Booth CR, Ludtke SJ, Riedl SJ, Shi Y, Wang X, Akey CW. A structure of the human apoptosome at 12.8 A resolution provides insights into this cell death platform. Structure. 2005 Nov; 13(11):1725-35.
    View in: PubMed
  17. Ménétret JF, Hegde RS, Heinrich SU, Chandramouli P, Ludtke SJ, Rapoport TA, Akey CW. Architecture of the ribosome-channel complex derived from native membranes. J Mol Biol. 2005 Apr 29; 348(2):445-57.
    View in: PubMed
  18. Namboodiri VM, Akey IV, Schmidt-Zachmann MS, Head JF, Akey CW. The structure and function of Xenopus NO38-core, a histone chaperone in the nucleolus. Structure. 2004 Dec; 12(12):2149-60.
    View in: PubMed
  19. Namboodiri VM, Schmidt-Zachmann MS, Head JF, Akey CW. Purification, crystallization and preliminary X-ray analysis of the N-terminal domain of NO38, a nucleolar protein from Xenopus laevis. Acta Crystallogr D Biol Crystallogr. 2004 Dec; 60(Pt 12 Pt 2):2325-7.
    View in: PubMed
  20. Clemons WM, Ménétret JF, Akey CW, Rapoport TA. Structural insight into the protein translocation channel. Curr Opin Struct Biol. 2004 Aug; 14(4):390-6.
    View in: PubMed
  21. Akey CW, Luger K. Histone chaperones and nucleosome assembly. Curr Opin Struct Biol. 2003 Feb; 13(1):6-14.
    View in: PubMed
  22. Namboodiri VM, Dutta S, Akey IV, Head JF, Akey CW. The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding. Structure. 2003 Feb; 11(2):175-86.
    View in: PubMed
  23. Morgan DG, Ménétret JF, Neuhof A, Rapoport TA, Akey CW. Structure of the mammalian ribosome-channel complex at 17A resolution. J Mol Biol. 2002 Dec 6; 324(4):871-86.
    View in: PubMed
  24. Acehan D, Jiang X, Morgan DG, Heuser JE, Wang X, Akey CW. Three-dimensional structure of the apoptosome: implications for assembly, procaspase-9 binding, and activation. Mol Cell. 2002 Feb; 9(2):423-32.
    View in: PubMed
  25. Dutta S, Akey IV, Dingwall C, Hartman KL, Laue T, Nolte RT, Head JF, Akey CW. The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly. Mol Cell. 2001 Oct; 8(4):841-53.
    View in: PubMed
  26. Menetret JF, Neuhof A, Morgan DG, Plath K, Radermacher M, Rapoport TA, Akey CW. The structure of ribosome-channel complexes engaged in protein translocation. Mol Cell. 2000 Nov; 6(5):1219-32.
    View in: PubMed
  27. Morgan DG, Ménétret JF, Radermacher M, Neuhof A, Akey IV, Rapoport TA, Akey CW. A comparison of the yeast and rabbit 80 S ribosome reveals the topology of the nascent chain exit tunnel, inter-subunit bridges and mammalian rRNA expansion segments. J Mol Biol. 2000 Aug 11; 301(2):301-21.
    View in: PubMed
  28. Meyer TH, Ménétret JF, Breitling R, Miller KR, Akey CW, Rapoport TA. The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex. J Mol Biol. 1999 Jan 29; 285(4):1789-800.
    View in: PubMed
  29. Kiseleva E, Goldberg MW, Allen TD, Akey CW. Active nuclear pore complexes in Chironomus: visualization of transporter configurations related to mRNP export. J Cell Sci. 1998 Jan; 111 ( Pt 2):223-36.
    View in: PubMed
  30. Yang Q, Rout MP, Akey CW. Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell. 1998 Jan; 1(2):223-34.
    View in: PubMed
  31. Bullitt E, Rout MP, Kilmartin JV, Akey CW. The yeast spindle pole body is assembled around a central crystal of Spc42p. Cell. 1997 Jun 27; 89(7):1077-86.
    View in: PubMed
  32. Hanein D, Matlack KE, Jungnickel B, Plath K, Kalies KU, Miller KR, Rapoport TA, Akey CW. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation. Cell. 1996 Nov 15; 87(4):721-32.
    View in: PubMed
  33. Akey CW. Structural plasticity of the nuclear pore complex. J Mol Biol. 1995 Apr 28; 248(2):273-93.
    View in: PubMed
  34. Akey CW, Radermacher M. Architecture of the Xenopus nuclear pore complex revealed by three-dimensional cryo-electron microscopy. J Cell Biol. 1993 Jul; 122(1):1-19.
    View in: PubMed
  35. Akey CW. Probing the structure and function of the nuclear pore complex. Semin Cell Biol. 1991 Jun; 2(3):167-77.
    View in: PubMed
  36. Akey CW. Visualization of transport-related configurations of the nuclear pore transporter. Biophys J. 1990 Aug; 58(2):341-55.
    View in: PubMed
  37. Akey CW. Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy. J Cell Biol. 1989 Sep; 109(3):955-70.
    View in: PubMed
  38. Akey CW, Goldfarb DS. Protein import through the nuclear pore complex is a multistep process. J Cell Biol. 1989 Sep; 109(3):971-82.
    View in: PubMed
  39. Akey CW, Crepeau RH, Edelstein SJ. The innermost chorionic layer of Drosophila. II. Three-dimensional structure determination of the 90 degrees crystal form by electron microscopy. J Mol Biol. 1987 Feb 20; 193(4):685-92.
    View in: PubMed
  40. Akey CW, Edelstein SJ. The innermost chorionic layer of Drosophila. I. The role of chorin octamers in the formation of a family of interdigitating crystalline plates. J Mol Biol. 1987 Feb 20; 193(4):673-83.
    View in: PubMed
  41. Akey CW, Dunn SD, Spitsberg V, Edelstein SJ. Electron microscopy of single molecules and crystals of F1-ATPases. Methods Enzymol. 1986; 126:434-46.
    View in: PubMed
  42. Akey CW, Szalay M, Edelstein SJ. Trigonal catalase crystals: a new molecular packing assignment obtained from sections preserved with tannic acid. Ultramicroscopy. 1984; 13(1-2):103-11.
    View in: PubMed
  43. Akey CW, Spitsberg V, Edelstein SJ. Electron microscopy of beef heart F1-ATPase crystals. J Biol Chem. 1983 Mar 10; 258(5):3222-9.
    View in: PubMed
  44. Akey CW, Edelstein SJ. Equivalence of the projected structure of thin catalase crystals preserved for electron microscopy by negative stain, glucose or embedding in the presence of tannic acid. J Mol Biol. 1983 Feb 5; 163(4):575-612.
    View in: PubMed
  45. Akey CW, Crepeau RH, Dunn SD, McCarty RE, Edelstein SJ. Electron microscopy and single molecule averaging of subunit-deficient F1-ATPases from Escherichia coli and spinach chloroplasts. EMBO J. 1983; 2(8):1409-15.
    View in: PubMed
  46. Akey CW, Moffat K, Wharton DC, Edelstein SJ. Characterization of crystals of a cytochrome oxidase (nitrite reductase) from Pseudomonas aeruginosa by x-ray diffraction and electron microscopy. J Mol Biol. 1980 Jan 5; 136(1):19-43.
    View in: PubMed
  47. Lykins LF, Akey CW, Christian EG, Duval GE, Topham RW. Dissociation and reconstitution of human ferroxidase II. Biochemistry. 1977 Feb 22; 16(4):693-8.
    View in: PubMed
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